Rh1AG333300

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0001364
Physical Location & Seq
Forward (+)
56810801 .. 56812114
1314 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG333300.1

Sequence Viewer

Length: 1155 bp
ATGACGAGTATCGAGCCTTTCAACAGGTTGGTGAAGCTTGCGGCCAGAGCGTTCTACGATGATGATATAGCAACGTATTCATCGAAAGGGGCTAATTCTCAACTCAAGAGTGGCCGGAGCGTCGAAAATAGAGGAATCGGCGTGGTTGTGCTGGAAGCGCTCTCGAGAAGACAATGGGTAAGAGAAGAAGATTTGGCCAAGGAGTTGAAACTCAACTCGAAGCAACTTCGTCGTACTTTGCAATTTTTCGAGGAGGAAAAACTGGTCACTCGAGACCATAGGAAAGAACGACATGCAAAGGAAGAAGGGGAAGAGAAAATAAAGTTGCACACTTACTCTTATTGCTGTCTGGACTATGCTCAGATGTACGATGTAGTGAGGTACAGACTGCATCGCATGAAGAAAAGACTGAAAGATGCATTGGAGGACAAGAACACAGTACAACAATACTTATGCCACAATTGTGGGAACAGATACAACGCTTTGGATGCAATGCGATTGATTTCGATGGAGGATGATGAGTACTTTCACTGTGAAAGATGCAATGGGAAACTTGTGGTGGTTGACACTAACAATGAGCTAGCTTCTCCACAAGTAGGAGATGAAGAAAGCAATATTTCAAGGAGACAAAGGCGTGAGAAATCGAAAGTCATGCTTCGAAATATGGAGGAACAACTGAAGCCTTTAATGGAACAACTTAATAGGGTTAAGGATTTACCTGTTCTTCAGTTTAGAACACTCCGAGATTGGGAAGCGCGAGCTAAATCTTCTGATGGACATGATTTTGGAGGTACAAAGGTTGAAGTTGAAGTTACTTGGGTTAAAGAAGACAAGGGCGACGATATTAAATCGGAATTAGATGATCCTACTACAAGTTTGGGGAAAACTGTGCCACCTTGGATGAACAAGCAAGGGATGAATAACCTTCCAAATCACGAACAACATGGACATTTTTCAGATGACATAAAGGCAAATACTCATGAAGATACTGAGAGCAAGAGTACTTTACAGGAGGAGTATTATAAAGCTTATTATCGTGCTCTAATACAAAGACAACAACAAGAAGAAGCAAATATTCACGAGGTTGGCATGAAGTCAAAATGTGATCAGAAAGAAGAAGACGATGAAGATGATATTGACTGGCAGGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

384

Amino Acids

45.1

Weight (kDa)

5.68

Isoelectric Point (pI)

49.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 48 - 144 6.5e-12 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1023
AccII CGCG 1 cut(s) 757
AciI CCGC 1 cut(s) 41
AclWI GGATC 1 cut(s) 857
AcoI YGGCCR 3 cut(s) 42, 112, 195
AcuI CTGAAG 2 cut(s) 698, 710
AfaI GTAC 7 cut(s) 235, 368, 383, 441, 524, 793, 1003
AfeI AGCGCT 1 cut(s) 159
AfiI CCNNNNNNNGG 2 cut(s) 596, 748
AgsI TTSAA 5 cut(s) 22, 208, 621, 803, 809
AjuI GAANNNNNNNTTGG 2 cut(s) 404, 436
AleI CACNNNNGTG 1 cut(s) 462
AluBI AGCT 5 cut(s) 37, 580, 584, 761, 1028
AluI AGCT 5 cut(s) 37, 580, 584, 761, 1028
Alw21I GWGCWC 1 cut(s) 1042
Alw26I GTCTC 2 cut(s) 267, 619
AlwI GGATC 1 cut(s) 857
Ama87I CYCGRG 2 cut(s) 163, 270
Aor51HI AGCGCT 1 cut(s) 159
AoxI GGCC 3 cut(s) 42, 112, 195
AspLEI GCGC 2 cut(s) 160, 757
AsuHPI GGTGA 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 658
AsuNHI GCTAGC 1 cut(s) 580
AvaI CYCGRG 2 cut(s) 163, 270
BaeI ACNNNNGTAYC 2 cut(s) 783, 816
BalI TGGCCA 1 cut(s) 197
BauI CACGAG 1 cut(s) 1079
BbsI GAAGAC 3 cut(s) 175, 834, 1125
Bbv12I GWGCWC 1 cut(s) 1042
BccI CCATC 2 cut(s) 502, 767
BcgI CGANNNNNNTGC 4 cut(s) 212, 246, 634, 668
BclI TGATCA 1 cut(s) 1105
BcoDI GTCTC 2 cut(s) 267, 619
BfaI CTAG 1 cut(s) 581
BfoI RGCGCY 1 cut(s) 161
BisI GCNGC 1 cut(s) 42
BlsI GCNGC 1 cut(s) 43
BmcAI AGTACT 2 cut(s) 524, 1003
BmeT110I CYCGRG 2 cut(s) 163, 270
BmsI GCATC 4 cut(s) 400, 406, 478, 530
BmtI GCTAGC 1 cut(s) 584
BpiI GAAGAC 3 cut(s) 175, 834, 1125
Bpu14I TTCGAA 1 cut(s) 658
BpuEI CTTGAG 1 cut(s) 89
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 2 cut(s) 198, 896
Bsc4I CCNNNNNNNGG 2 cut(s) 596, 748
Bse1I ACTGG 2 cut(s) 267, 1145
Bse3DI GCAATG 2 cut(s) 498, 550
BseDI CCNNGG 2 cut(s) 198, 896
BseGI GGATG 4 cut(s) 493, 520, 906, 921
BseLI CCNNNNNNNGG 2 cut(s) 596, 748
BseMI GCAATG 2 cut(s) 498, 550
BseMII CTCAG 2 cut(s) 374, 981
BseNI ACTGG 2 cut(s) 267, 1145
BseRI GAGGAG 2 cut(s) 266, 1028
Bsh1236I CGCG 1 cut(s) 757
BshFI GGCC 3 cut(s) 44, 114, 197
BsiHKAI GWGCWC 1 cut(s) 1042
BsiHKCI CYCGRG 2 cut(s) 163, 270
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 2 cut(s) 596, 748
BsmAI GTCTC 2 cut(s) 267, 619
BsnI GGCC 3 cut(s) 44, 114, 197
Bso31I GGTCTC 1 cut(s) 267
BsoBI CYCGRG 2 cut(s) 163, 270
Bsp119I TTCGAA 1 cut(s) 658
Bsp1286I GDGCHC 1 cut(s) 1042
Bsp143I GATC 2 cut(s) 862, 1105
BspACI CCGC 1 cut(s) 41
BspANI GGCC 3 cut(s) 44, 114, 197
BspCNI CTCAG 2 cut(s) 373, 982
BspFNI CGCG 1 cut(s) 757
BspHI TCATGA 1 cut(s) 979
BspOI GCTAGC 1 cut(s) 584
BspPI GGATC 1 cut(s) 857
BspT104I TTCGAA 1 cut(s) 658
BspTNI GGTCTC 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 498, 550
BsrI ACTGG 2 cut(s) 267, 1145
BssECI CCNNGG 2 cut(s) 198, 896
BssMI GATC 2 cut(s) 862, 1105
BssSI CACGAG 1 cut(s) 1079
BssT1I CCWWGG 2 cut(s) 198, 896
Bst2BI CACGAG 1 cut(s) 1079
Bst4CI ACNGT 3 cut(s) 439, 533, 889
Bst6I CTCTTC 1 cut(s) 306
BstBI TTCGAA 1 cut(s) 658
BstC8I GCNNGC 3 cut(s) 39, 582, 759
BstDEI CTNAG 2 cut(s) 360, 990
BstF5I GGATG 4 cut(s) 493, 520, 906, 921
BstFNI CGCG 1 cut(s) 757
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 2 cut(s) 160, 757
BstKTI GATC 2 cut(s) 865, 1108
BstMAI GTCTC 2 cut(s) 267, 619
BstMBI GATC 2 cut(s) 862, 1105
BstMWI GCNNNNNNNGC 3 cut(s) 47, 157, 488
BstNSI RCATGY 1 cut(s) 296
BstUI CGCG 1 cut(s) 757
BstV2I GAAGAC 3 cut(s) 175, 834, 1125
BstXI CCANNNNNNTGG 1 cut(s) 464
BsuRI GGCC 3 cut(s) 44, 114, 197
BtgZI GCGATG 1 cut(s) 377
BtsCI GGATG 4 cut(s) 493, 520, 906, 921
BtsIMutI CAGTG 1 cut(s) 529
Cac8I GCNNGC 3 cut(s) 39, 582, 759
CciI TCATGA 1 cut(s) 979
CfoI GCGC 2 cut(s) 160, 757
CseI GACGC 1 cut(s) 109
Csp6I GTAC 7 cut(s) 234, 367, 382, 440, 523, 792, 1002
CviAII CATG 7 cut(s) 293, 397, 652, 779, 944, 980, 1090
CviQI GTAC 7 cut(s) 234, 367, 382, 440, 523, 792, 1002
DdeI CTNAG 2 cut(s) 360, 990
DpnI GATC 2 cut(s) 864, 1107
DpnII GATC 2 cut(s) 862, 1105
EaeI YGGCCR 3 cut(s) 42, 112, 195
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
Eco130I CCWWGG 2 cut(s) 198, 896
Eco31I GGTCTC 1 cut(s) 267
Eco47III AGCGCT 1 cut(s) 159
Eco57I CTGAAG 2 cut(s) 698, 710
Eco88I CYCGRG 2 cut(s) 163, 270
EcoT14I CCWWGG 2 cut(s) 198, 896
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 198, 896
FaeI CATG 7 cut(s) 296, 400, 655, 782, 947, 983, 1093
FalI AAGNNNNNCTT 2 cut(s) 639, 671
FatI CATG 7 cut(s) 292, 396, 651, 778, 943, 979, 1089
FbaI TGATCA 1 cut(s) 1105
Fnu4HI GCNGC 1 cut(s) 42
FokI GGATG 4 cut(s) 500, 527, 913, 928
Fsp4HI GCNGC 1 cut(s) 42
FspBI CTAG 1 cut(s) 581
GlaI GCGC 2 cut(s) 159, 756
GluI GCNGC 1 cut(s) 42
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 3 cut(s) 44, 114, 197
HapII CCGG 1 cut(s) 115
HgaI GACGC 1 cut(s) 109
HhaI GCGC 2 cut(s) 160, 757
Hin1II CATG 7 cut(s) 296, 400, 655, 782, 947, 983, 1093
Hin6I GCGC 2 cut(s) 158, 755
HinP1I GCGC 2 cut(s) 158, 755
HincII GTYRAC 1 cut(s) 565
HindII GTYRAC 1 cut(s) 565
HindIII AAGCTT 2 cut(s) 35, 1026
HinfI GANTC 1 cut(s) 135
HpaII CCGG 1 cut(s) 115
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 1 cut(s) 565
Hpy188I TCNGA 6 cut(s) 363, 743, 772, 853, 958, 1110
Hpy188III TCNNGA 8 cut(s) 106, 163, 165, 272, 350, 935, 980, 1079
Hpy8I GTNNAC 1 cut(s) 565
Hpy99I CGWCG 3 cut(s) 125, 234, 842
HpyAV CCTTC 3 cut(s) 299, 935, 1142
HpyCH4III ACNGT 3 cut(s) 439, 533, 889
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 7 cut(s) 241, 296, 328, 391, 419, 491, 543
HpyF10VI GCNNNNNNNGC 3 cut(s) 47, 157, 488
HpyF3I CTNAG 2 cut(s) 360, 990
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 7 cut(s) 296, 400, 655, 782, 947, 983, 1093
HspAI GCGC 2 cut(s) 158, 755
Ksp22I TGATCA 1 cut(s) 1105
Kzo9I GATC 2 cut(s) 862, 1105
LmnI GCTCC 1 cut(s) 117
LweI GCATC 4 cut(s) 400, 406, 478, 530
MaeI CTAG 1 cut(s) 581
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 2 cut(s) 265, 811
MalI GATC 2 cut(s) 864, 1107
MboI GATC 2 cut(s) 862, 1105
MfeI CAATTG 1 cut(s) 460
MhlI GDGCHC 1 cut(s) 1042
MlsI TGGCCA 1 cut(s) 197
MluCI AATT 4 cut(s) 94, 242, 460, 854
MluNI TGGCCA 1 cut(s) 197
Mox20I TGGCCA 1 cut(s) 197
Mph1103I ATGCAT 1 cut(s) 421
MscI TGGCCA 1 cut(s) 197
MseI TTAA 5 cut(s) 686, 699, 708, 822, 846
MslI CAYNNNNRTG 1 cut(s) 462
Msp20I TGGCCA 1 cut(s) 197
MspI CCGG 1 cut(s) 115
MunI CAATTG 1 cut(s) 460
MvnI CGCG 1 cut(s) 757
MwoI GCNNNNNNNGC 3 cut(s) 47, 157, 488
NdeII GATC 2 cut(s) 862, 1105
NheI GCTAGC 1 cut(s) 580
NlaIII CATG 7 cut(s) 296, 400, 655, 782, 947, 983, 1093
NmuCI GTSAC 1 cut(s) 265
NsiI ATGCAT 1 cut(s) 421
NspI RCATGY 1 cut(s) 296
NspV TTCGAA 1 cut(s) 658
OliI CACNNNNGTG 1 cut(s) 462
PaeR7I CTCGAG 2 cut(s) 163, 270
PagI TCATGA 1 cut(s) 979
PcsI WCGNNNNNNNCGW 1 cut(s) 80
PfeI GAWTC 1 cut(s) 135
PkrI GCNGC 1 cut(s) 43
PsiI TTATAA 1 cut(s) 1023
RsaI GTAC 7 cut(s) 235, 368, 383, 441, 524, 793, 1003
RsaNI GTAC 7 cut(s) 234, 367, 382, 440, 523, 792, 1002
RseI CAYNNNNRTG 1 cut(s) 462
SaqAI TTAA 5 cut(s) 686, 699, 708, 822, 846
SatI GCNGC 1 cut(s) 42
Sau3AI GATC 2 cut(s) 862, 1105
ScaI AGTACT 2 cut(s) 524, 1003
SduI GDGCHC 1 cut(s) 1042
SfaNI GCATC 4 cut(s) 400, 406, 478, 530
Sfr274I CTCGAG 2 cut(s) 163, 270
SfuI TTCGAA 1 cut(s) 658
SlaI CTCGAG 2 cut(s) 163, 270
SmiMI CAYNNNNRTG 1 cut(s) 462
SmlI CTYRAG 3 cut(s) 104, 163, 270
SmoI CTYRAG 3 cut(s) 104, 163, 270
Sse9I AATT 4 cut(s) 94, 242, 460, 854
SsiI CCGC 1 cut(s) 41
SspI AATATT 2 cut(s) 616, 1075
SspMI CTAG 1 cut(s) 581
StyI CCWWGG 2 cut(s) 198, 896
TaaI ACNGT 3 cut(s) 439, 533, 889
TaiI ACGT 1 cut(s) 77
TasI AATT 4 cut(s) 94, 242, 460, 854
TatI WGTACW 3 cut(s) 439, 522, 1001
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 5 cut(s) 686, 699, 708, 822, 846
Tru9I TTAA 5 cut(s) 686, 699, 708, 822, 846
TscAI CASTG 1 cut(s) 536
TseFI GTSAC 1 cut(s) 265
Tsp45I GTSAC 1 cut(s) 265
TspDTI ATGAA 8 cut(s) 69, 413, 618, 917, 932, 996, 1106, 1140
TspRI CASTG 1 cut(s) 536
XceI RCATGY 1 cut(s) 296
XhoI CTCGAG 2 cut(s) 163, 270
XspI CTAG 1 cut(s) 581
ZrmI AGTACT 2 cut(s) 524, 1003
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.