RLG00000027395

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0001364
Physical Location & Seq
Reverse (-)
11209046 .. 11210200
1155 bp
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UTR
Exon/CDS
Intron
RLM00000027395

Sequence Viewer

Length: 969 bp
ATGACGAGTATCGAGCCTTTCAACAGGTTGGTGAAGCTTGCGGCCAGAGCGTTCTACGATGATGATATAGCAACGTATTCATCAAAAGGGGCTAATTCTCAGCTCAAGAGTGGCCGGAGCGTCGAAAATAGAGGAATCGGCGTGGTTGTGCTGGAAGCGCTCTCGAGAAGACAATGGATGTACGATGTAGTGAGGTACAGACTGCATCGCATGAAGAAAAGACTGAAAGATGCATTGGAGGACAAGAACACAGTACAACAATACTTATGCCACAATTGTGGGAACAGATACAACGCTTTGGATGCGCTGCGATTGATTTCGATGGAGGATGATGAGTACTTTCACTGTGAAAGATGCAATGGGAAACTTATGGTGGTTGAGACTAACAATGAGCTAGCTTCTCCACAAGTAGGAGATGAAGAAAGCAATATTTCAAGGAGACAAAGGCGTGAGAAATCGAAAGTCATGCTTCGAAATATGGAGGAACAACTGAAGCCTTTAATGGAACAAATTAATAGGGTTAAGGATTTACCTGTTCCTCAGTTTGGAACACTCCGAGATTGGGAAGCGCGAGCTAAATCTTCTGATGGACATGATTTTGGAGGTACAAAGGTTGAAGTTGAAGTTACTAGGGTTAAAGAAGACAAGGGCGACGATATTAAATCGGAATTAGATGATCCTACTACAAGTTTGGGGAAAACTGTGCCACCTTGGATGAACAAGCAAGGGATGAATAACCTTCCAAATCACGAACAACATGGACATTTTTCAGATGACATAAAGGCAAACACTCATGAAGATAATGAGAGCAAGAGTACTTTACAGGAGGAGTATTATAAAGCTTATTATCGTTTTCTAATACAAAGACAAGAAGAAGAAGAAGCAAATATTCGCGAGGTTGGCATGAAGTCAAAATGTGATCAGAAAGAAGGAGAGGATGAAGATGATATTGACTGGCAGGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

323

Amino Acids

37.45

Weight (kDa)

5.39

Isoelectric Point (pI)

50.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 837
AccII CGCG 2 cut(s) 571, 894
AciI CCGC 1 cut(s) 41
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 2 cut(s) 42, 112
AcuI CTGAAG 1 cut(s) 512
AfaI GTAC 6 cut(s) 182, 197, 255, 338, 607, 817
AfeI AGCGCT 1 cut(s) 159
AfiI CCNNNNNNNGG 3 cut(s) 410, 545, 562
AgsI TTSAA 4 cut(s) 22, 435, 617, 623
AjuI GAANNNNNNNTTGG 2 cut(s) 218, 250
AleI CACNNNNGTG 1 cut(s) 276
AluBI AGCT 6 cut(s) 37, 103, 394, 398, 575, 842
AluI AGCT 6 cut(s) 37, 103, 394, 398, 575, 842
Alw26I GTCTC 2 cut(s) 374, 433
AlwI GGATC 1 cut(s) 671
Ama87I CYCGRG 1 cut(s) 163
Aor51HI AGCGCT 1 cut(s) 159
AoxI GGCC 2 cut(s) 42, 112
ApeKI GCWGC 1 cut(s) 307
AseI ATTAAT 1 cut(s) 513
AspLEI GCGC 3 cut(s) 160, 307, 571
AsuHPI GGTGA 1 cut(s) 43
AsuII TTCGAA 1 cut(s) 472
AsuNHI GCTAGC 1 cut(s) 394
AvaI CYCGRG 1 cut(s) 163
BaeI ACNNNNGTAYC 2 cut(s) 597, 630
BbsI GAAGAC 2 cut(s) 175, 648
BbvI GCAGC 1 cut(s) 294
BccI CCATC 2 cut(s) 316, 581
BcgI CGANNNNNNTGC 2 cut(s) 448, 482
BclI TGATCA 1 cut(s) 919
BcoDI GTCTC 2 cut(s) 374, 433
BfaI CTAG 2 cut(s) 395, 630
BfoI RGCGCY 1 cut(s) 161
BisI GCNGC 2 cut(s) 42, 308
BlsI GCNGC 2 cut(s) 43, 309
BmcAI AGTACT 2 cut(s) 338, 817
BmeT110I CYCGRG 1 cut(s) 163
BmsI GCATC 4 cut(s) 214, 220, 292, 344
BmtI GCTAGC 1 cut(s) 398
BpiI GAAGAC 2 cut(s) 175, 648
Bpu14I TTCGAA 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 89
BsaJI CCNNGG 1 cut(s) 710
Bsc4I CCNNNNNNNGG 3 cut(s) 410, 545, 562
Bse1I ACTGG 1 cut(s) 959
Bse3DI GCAATG 1 cut(s) 364
BseDI CCNNGG 1 cut(s) 710
BseGI GGATG 6 cut(s) 183, 307, 334, 720, 735, 943
BseLI CCNNNNNNNGG 3 cut(s) 410, 545, 562
BseMI GCAATG 1 cut(s) 364
BseMII CTCAG 2 cut(s) 113, 554
BseNI ACTGG 1 cut(s) 959
BseRI GAGGAG 1 cut(s) 842
BseXI GCAGC 1 cut(s) 294
Bsh1236I CGCG 2 cut(s) 571, 894
BshFI GGCC 2 cut(s) 44, 114
BsiHKCI CYCGRG 1 cut(s) 163
BsiSI CCGG 1 cut(s) 115
BslI CCNNNNNNNGG 3 cut(s) 410, 545, 562
BsmAI GTCTC 2 cut(s) 374, 433
BsnI GGCC 2 cut(s) 44, 114
BsoBI CYCGRG 1 cut(s) 163
Bsp119I TTCGAA 1 cut(s) 472
Bsp143I GATC 2 cut(s) 676, 919
Bsp68I TCGCGA 1 cut(s) 894
BspACI CCGC 1 cut(s) 41
BspANI GGCC 2 cut(s) 44, 114
BspCNI CTCAG 2 cut(s) 112, 553
BspFNI CGCG 2 cut(s) 571, 894
BspHI TCATGA 1 cut(s) 793
BspOI GCTAGC 1 cut(s) 398
BspPI GGATC 1 cut(s) 671
BspT104I TTCGAA 1 cut(s) 472
BsrDI GCAATG 1 cut(s) 364
BsrI ACTGG 1 cut(s) 959
BssECI CCNNGG 1 cut(s) 710
BssMI GATC 2 cut(s) 676, 919
BssT1I CCWWGG 1 cut(s) 710
Bst4CI ACNGT 3 cut(s) 253, 347, 703
BstBI TTCGAA 1 cut(s) 472
BstC8I GCNNGC 3 cut(s) 39, 396, 573
BstDEI CTNAG 2 cut(s) 99, 540
BstF5I GGATG 6 cut(s) 183, 307, 334, 720, 735, 943
BstFNI CGCG 2 cut(s) 571, 894
BstH2I RGCGCY 1 cut(s) 161
BstHHI GCGC 3 cut(s) 160, 307, 571
BstKTI GATC 2 cut(s) 679, 922
BstMAI GTCTC 2 cut(s) 374, 433
BstMBI GATC 2 cut(s) 676, 919
BstMWI GCNNNNNNNGC 4 cut(s) 47, 157, 302, 900
BstUI CGCG 2 cut(s) 571, 894
BstV1I GCAGC 1 cut(s) 294
BstV2I GAAGAC 2 cut(s) 175, 648
BstXI CCANNNNNNTGG 1 cut(s) 278
BsuRI GGCC 2 cut(s) 44, 114
BtgZI GCGATG 1 cut(s) 191
BtsCI GGATG 6 cut(s) 183, 307, 334, 720, 735, 943
BtsIMutI CAGTG 1 cut(s) 343
BtuMI TCGCGA 1 cut(s) 894
Cac8I GCNNGC 3 cut(s) 39, 396, 573
CciI TCATGA 1 cut(s) 793
CfoI GCGC 3 cut(s) 160, 307, 571
CseI GACGC 1 cut(s) 109
Csp6I GTAC 6 cut(s) 181, 196, 254, 337, 606, 816
CviAII CATG 6 cut(s) 211, 466, 593, 758, 794, 904
CviQI GTAC 6 cut(s) 181, 196, 254, 337, 606, 816
DdeI CTNAG 2 cut(s) 99, 540
DpnI GATC 2 cut(s) 678, 921
DpnII GATC 2 cut(s) 676, 919
EaeI YGGCCR 2 cut(s) 42, 112
Eco130I CCWWGG 1 cut(s) 710
Eco47III AGCGCT 1 cut(s) 159
Eco57I CTGAAG 1 cut(s) 512
Eco88I CYCGRG 1 cut(s) 163
EcoT14I CCWWGG 1 cut(s) 710
EcoT22I ATGCAT 1 cut(s) 235
ErhI CCWWGG 1 cut(s) 710
FaeI CATG 6 cut(s) 214, 469, 596, 761, 797, 907
FalI AAGNNNNNCTT 2 cut(s) 453, 485
FatI CATG 6 cut(s) 210, 465, 592, 757, 793, 903
FbaI TGATCA 1 cut(s) 919
Fnu4HI GCNGC 2 cut(s) 42, 308
FokI GGATG 6 cut(s) 190, 314, 341, 727, 742, 950
Fsp4HI GCNGC 2 cut(s) 42, 308
FspBI CTAG 2 cut(s) 395, 630
GlaI GCGC 3 cut(s) 159, 306, 570
GluI GCNGC 2 cut(s) 42, 308
HaeII RGCGCY 1 cut(s) 161
HaeIII GGCC 2 cut(s) 44, 114
HapII CCGG 1 cut(s) 115
HgaI GACGC 1 cut(s) 109
HhaI GCGC 3 cut(s) 160, 307, 571
Hin1II CATG 6 cut(s) 214, 469, 596, 761, 797, 907
Hin6I GCGC 3 cut(s) 158, 305, 569
HinP1I GCGC 3 cut(s) 158, 305, 569
HindIII AAGCTT 2 cut(s) 35, 840
HinfI GANTC 1 cut(s) 135
HpaII CCGG 1 cut(s) 115
HphI GGTGA 1 cut(s) 43
Hpy188I TCNGA 5 cut(s) 557, 586, 667, 772, 924
Hpy188III TCNNGA 6 cut(s) 106, 163, 165, 749, 794, 893
Hpy99I CGWCG 2 cut(s) 125, 656
HpyAV CCTTC 3 cut(s) 749, 923, 956
HpyCH4III ACNGT 3 cut(s) 253, 347, 703
HpyCH4IV ACGT 1 cut(s) 74
HpyCH4V TGCA 3 cut(s) 205, 233, 357
HpyF10VI GCNNNNNNNGC 4 cut(s) 47, 157, 302, 900
HpyF3I CTNAG 2 cut(s) 99, 540
HpySE526I ACGT 1 cut(s) 74
Hsp92II CATG 6 cut(s) 214, 469, 596, 761, 797, 907
HspAI GCGC 3 cut(s) 158, 305, 569
Ksp22I TGATCA 1 cut(s) 919
Kzo9I GATC 2 cut(s) 676, 919
LmnI GCTCC 1 cut(s) 117
LpnPI CCDG 8 cut(s) 10, 58, 128, 137, 546, 809, 940, 944
Lsp1109I GCAGC 1 cut(s) 294
LweI GCATC 4 cut(s) 214, 220, 292, 344
MaeI CTAG 2 cut(s) 395, 630
MaeII ACGT 1 cut(s) 74
MaeIII GTNAC 1 cut(s) 625
MalI GATC 2 cut(s) 678, 921
MboI GATC 2 cut(s) 676, 919
MfeI CAATTG 1 cut(s) 274
MluCI AATT 4 cut(s) 94, 274, 510, 668
Mph1103I ATGCAT 1 cut(s) 235
MseI TTAA 5 cut(s) 500, 513, 522, 636, 660
MslI CAYNNNNRTG 1 cut(s) 276
MspI CCGG 1 cut(s) 115
MunI CAATTG 1 cut(s) 274
MvnI CGCG 2 cut(s) 571, 894
MwoI GCNNNNNNNGC 4 cut(s) 47, 157, 302, 900
NdeII GATC 2 cut(s) 676, 919
NheI GCTAGC 1 cut(s) 394
NlaIII CATG 6 cut(s) 214, 469, 596, 761, 797, 907
NruI TCGCGA 1 cut(s) 894
NsiI ATGCAT 1 cut(s) 235
NspV TTCGAA 1 cut(s) 472
OliI CACNNNNGTG 1 cut(s) 276
PaeR7I CTCGAG 1 cut(s) 163
PagI TCATGA 1 cut(s) 793
PfeI GAWTC 1 cut(s) 135
PkrI GCNGC 2 cut(s) 43, 309
PshBI ATTAAT 1 cut(s) 513
PsiI TTATAA 1 cut(s) 837
RruI TCGCGA 1 cut(s) 894
RsaI GTAC 6 cut(s) 182, 197, 255, 338, 607, 817
RsaNI GTAC 6 cut(s) 181, 196, 254, 337, 606, 816
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 5 cut(s) 500, 513, 522, 636, 660
SatI GCNGC 2 cut(s) 42, 308
Sau3AI GATC 2 cut(s) 676, 919
ScaI AGTACT 2 cut(s) 338, 817
SfaNI GCATC 4 cut(s) 214, 220, 292, 344
Sfr274I CTCGAG 1 cut(s) 163
SfuI TTCGAA 1 cut(s) 472
SlaI CTCGAG 1 cut(s) 163
SmiMI CAYNNNNRTG 1 cut(s) 276
SmlI CTYRAG 2 cut(s) 104, 163
SmoI CTYRAG 2 cut(s) 104, 163
Sse9I AATT 4 cut(s) 94, 274, 510, 668
SsiI CCGC 1 cut(s) 41
SspI AATATT 2 cut(s) 430, 889
SspMI CTAG 2 cut(s) 395, 630
StyI CCWWGG 1 cut(s) 710
TaaI ACNGT 3 cut(s) 253, 347, 703
TaiI ACGT 1 cut(s) 77
TaqI TCGA 6 cut(s) 12, 123, 164, 320, 458, 472
TasI AATT 4 cut(s) 94, 274, 510, 668
TatI WGTACW 3 cut(s) 253, 336, 815
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 135
Tru1I TTAA 5 cut(s) 500, 513, 522, 636, 660
Tru9I TTAA 5 cut(s) 500, 513, 522, 636, 660
TscAI CASTG 1 cut(s) 350
TseI GCWGC 1 cut(s) 307
TspDTI ATGAA 8 cut(s) 69, 227, 432, 731, 746, 810, 920, 954
TspRI CASTG 1 cut(s) 350
VspI ATTAAT 1 cut(s) 513
XhoI CTCGAG 1 cut(s) 163
XspI CTAG 2 cut(s) 395, 630
ZrmI AGTACT 2 cut(s) 338, 817
Zsp2I ATGCAT 1 cut(s) 235
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.