Rh7AG079300

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001364
Physical Location & Seq
Forward (+)
5694857 .. 5699593
4737 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG079300.1

Sequence Viewer

Length: 1107 bp
ATGACAGCTAAGGGTGCAAAGATATTTAGTGCTGCAGTTGCGGCAACAGCTGACGGGCAACAACATACAAAAGAAGGGGAAGAGAAAATTAAGTTGCACACTCACTCATACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCGTATGAGGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAATGCTTTGGATGCACTGCAACTCATCTCTTTGGAGGATGAGTACTTCCACTGCGAGAGTTGCAATGGGGAGCTTGTGGCTGAGAGTGACAAGCTAGCTTCTCAAGATGTAGGAGATGGAGATAGCAATGCAAGGAGGCAAAGGCGTGAAAAACTGAAAGACATGCTTCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGGGTGAAAGACTTACCTGCTCCTGAATTTGGAACTCTCCAAGCTTGGGAAGCACGAGCACATGCTCAACGACGTGCAGAGAATGGAGAATCTAGTGCCATGGATCCCTCCAAATCTTCACATGGGCTGGGGTACGGTGGAACGCCCATGCCTTTTCTTGGAGATACAAAAGTTGAAGTTGATTTTGGGAATGAAGGCAAGGGTAACGATGTCAAATCAGAAGCTGATATTACAGGCCAGAAAATTTTGCCACCTTGGATGATAAAGCAAGGGATGAACCTGACAAAGGAACAGCGTGGAGAGGTAAAGCAGGAGGCAAAGATGGATGGCACGTTAGCAGCTCCAGAGTATTCAGATGACAAAAAGTCAAATGCTGAAGATAATGATAAGGCTTTCCAGGATGAGTATTTCAAAGCCTATTACGCTGCTTTAATAAAAAGACAAGAAGAAGAAGCAGCAAAGAGTCGTCAGGTTGGCATGAAGTCAAAACGTGATATGAATGAAGGAGATGAAGGAGAAGATAATGTTGACTGGCAAGAGGCTCCAGTTGCAGGTGATGCCAATGGAAATTACAAGGTCAATGACTTAAACGTCGAAGCCGATGCTACTGCAGAGGATGAAGATGACATAGACTGGGAGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

368

Amino Acids

41.34

Weight (kDa)

4.8

Isoelectric Point (pI)

37.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1001
AasI GACNNNNNNGTC 1 cut(s) 1049
Acc36I ACCTGC 2 cut(s) 484, 1001
AciI CCGC 1 cut(s) 41
AclWI GGATC 2 cut(s) 557, 570
AcsI RAATTY 2 cut(s) 485, 702
AcuI CTGAAG 2 cut(s) 455, 855
AfaI GTAC 4 cut(s) 152, 290, 429, 593
AfiI CCNNNNNNNGG 6 cut(s) 233, 488, 505, 617, 745, 1010
AgsI TTSAA 3 cut(s) 416, 635, 871
AhdI GACNNNNNGTC 1 cut(s) 823
AjiI CACGTC 1 cut(s) 533
AjnI CCWGG 1 cut(s) 855
AjuI GAANNNNNNNTTGG 4 cut(s) 173, 205, 627, 659
AluBI AGCT 9 cut(s) 8, 50, 319, 340, 344, 433, 503, 683, 800
AluI AGCT 9 cut(s) 8, 50, 319, 340, 344, 433, 503, 683, 800
Alw21I GWGCWC 1 cut(s) 520
AlwI GGATC 2 cut(s) 557, 570
AlwNI CAGNNNCTG 1 cut(s) 683
AoxI GGCC 1 cut(s) 694
ApeKI GCWGC 4 cut(s) 32, 797, 884, 914
ApoI RAATTY 2 cut(s) 485, 702
AseI ATTAAT 1 cut(s) 456
AsuHPI GGTGA 2 cut(s) 475, 1025
AsuNHI GCTAGC 1 cut(s) 340
BaeI ACNNNNGTAYC 2 cut(s) 615, 648
BamHI GGATCC 1 cut(s) 562
BauI CACGAG 1 cut(s) 513
Bbv12I GWGCWC 1 cut(s) 520
BbvI GCAGC 4 cut(s) 19, 809, 871, 926
BccI CCATC 3 cut(s) 356, 775, 779
BcgI CGANNNNNNTGC 4 cut(s) 1043, 1049, 1077, 1083
BciT130I CCWGG 1 cut(s) 857
BfaI CTAG 2 cut(s) 341, 552
BfmI CTRYAG 2 cut(s) 33, 1068
BfuAI ACCTGC 2 cut(s) 484, 1001
BisI GCNGC 5 cut(s) 33, 42, 798, 885, 915
BlsI GCNGC 5 cut(s) 34, 43, 799, 886, 916
BmcAI AGTACT 1 cut(s) 290
Bme1390I CCNGG 1 cut(s) 857
BmeRI GACNNNNNGTC 1 cut(s) 823
BmgBI CACGTC 1 cut(s) 533
BmiI GGNNCC 2 cut(s) 564, 1002
BmrFI CCNGG 1 cut(s) 857
BmrI ACTGGG 1 cut(s) 1102
BmsI GCATC 3 cut(s) 247, 1006, 1051
BmtI GCTAGC 1 cut(s) 344
BmuI ACTGGG 1 cut(s) 1102
BpmI CTGGAG 2 cut(s) 786, 987
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 558, 713
BsaXI ACNNNNNCTCC 2 cut(s) 272, 302
Bsc4I CCNNNNNNNGG 6 cut(s) 233, 488, 505, 617, 745, 1010
Bse1I ACTGG 3 cut(s) 995, 1004, 1097
Bse3DI GCAATG 2 cut(s) 316, 379
BseBI CCWGG 1 cut(s) 857
BseDI CCNNGG 2 cut(s) 558, 713
BseGI GGATG 7 cut(s) 262, 289, 723, 738, 790, 865, 1081
BseLI CCNNNNNNNGG 6 cut(s) 233, 488, 505, 617, 745, 1010
BseMI GCAATG 2 cut(s) 316, 379
BseMII CTCAG 2 cut(s) 143, 318
BseNI ACTGG 3 cut(s) 995, 1004, 1097
BseXI GCAGC 4 cut(s) 19, 809, 871, 926
BseYI CCCAGC 1 cut(s) 586
BsgI GTGCAG 2 cut(s) 143, 555
BshFI GGCC 1 cut(s) 696
BsiHKAI GWGCWC 1 cut(s) 520
BslI CCNNNNNNNGG 6 cut(s) 233, 488, 505, 617, 745, 1010
BsnI GGCC 1 cut(s) 696
Bsp1286I GDGCHC 1 cut(s) 520
Bsp143I GATC 1 cut(s) 562
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 1 cut(s) 41
BspANI GGCC 1 cut(s) 696
BspCNI CTCAG 2 cut(s) 142, 319
BspLI GGNNCC 2 cut(s) 564, 1002
BspMAI CTGCAG 2 cut(s) 37, 1072
BspMI ACCTGC 2 cut(s) 484, 1001
BspOI GCTAGC 1 cut(s) 344
BspPI GGATC 2 cut(s) 557, 570
BsrDI GCAATG 2 cut(s) 316, 379
BsrI ACTGG 3 cut(s) 995, 1004, 1097
BssECI CCNNGG 2 cut(s) 558, 713
BssMI GATC 1 cut(s) 562
BssSI CACGAG 1 cut(s) 513
BssT1I CCWWGG 2 cut(s) 558, 713
Bst2BI CACGAG 1 cut(s) 513
Bst2UI CCWGG 1 cut(s) 857
Bst4CI ACNGT 4 cut(s) 164, 208, 233, 596
Bst6I CTCTTC 1 cut(s) 75
BstAPI GCANNNNNTGC 1 cut(s) 1016
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 3 cut(s) 9, 129, 327
BstDSI CCRYGG 1 cut(s) 558
BstENI CCTNNNNNAGG 1 cut(s) 743
BstF5I GGATG 7 cut(s) 262, 289, 723, 738, 790, 865, 1081
BstKTI GATC 1 cut(s) 565
BstMBI GATC 1 cut(s) 562
BstNI CCWGG 1 cut(s) 857
BstNSI RCATGY 2 cut(s) 412, 524
BstSCI CCNGG 1 cut(s) 855
BstSFI CTRYAG 2 cut(s) 33, 1068
BstV1I GCAGC 4 cut(s) 19, 809, 871, 926
BstX2I RGATCY 1 cut(s) 562
BstYI RGATCY 1 cut(s) 562
BsuRI GGCC 1 cut(s) 696
BtgI CCRYGG 1 cut(s) 558
BtrI CACGTC 1 cut(s) 533
BtsCI GGATG 7 cut(s) 262, 289, 723, 738, 790, 865, 1081
BtsI GCAGTG 2 cut(s) 260, 295
BtsIMutI CAGTG 2 cut(s) 260, 295
BveI ACCTGC 2 cut(s) 484, 1001
Cac8I GCNNGC 1 cut(s) 342
CaiI CAGNNNCTG 1 cut(s) 683
Csp6I GTAC 4 cut(s) 151, 289, 428, 592
CviAII CATG 6 cut(s) 409, 521, 559, 581, 607, 937
CviQI GTAC 4 cut(s) 151, 289, 428, 592
DdeI CTNAG 3 cut(s) 9, 129, 327
DpnI GATC 1 cut(s) 564
DpnII GATC 1 cut(s) 562
DrdI GACNNNNNNGTC 1 cut(s) 1049
DriI GACNNNNNGTC 1 cut(s) 823
DseDI GACNNNNNNGTC 1 cut(s) 1049
Eam1104I CTCTTC 1 cut(s) 75
Eam1105I GACNNNNNGTC 1 cut(s) 823
EarI CTCTTC 1 cut(s) 75
Eco130I CCWWGG 2 cut(s) 558, 713
Eco57I CTGAAG 2 cut(s) 455, 855
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoRII CCWGG 1 cut(s) 855
EcoT14I CCWWGG 2 cut(s) 558, 713
ErhI CCWWGG 2 cut(s) 558, 713
FaeI CATG 6 cut(s) 412, 524, 562, 584, 610, 940
FalI AAGNNNNNCTT 2 cut(s) 396, 428
FatI CATG 6 cut(s) 408, 520, 558, 580, 606, 936
Fnu4HI GCNGC 5 cut(s) 33, 42, 798, 885, 915
FokI GGATG 7 cut(s) 269, 296, 730, 745, 797, 872, 1088
Fsp4HI GCNGC 5 cut(s) 33, 42, 798, 885, 915
FspBI CTAG 2 cut(s) 341, 552
GluI GCNGC 5 cut(s) 33, 42, 798, 885, 915
GsaI CCCAGC 1 cut(s) 590
GsuI CTGGAG 2 cut(s) 786, 987
HaeIII GGCC 1 cut(s) 696
Hin1II CATG 6 cut(s) 412, 524, 562, 584, 610, 940
HincII GTYRAC 1 cut(s) 988
HindII GTYRAC 1 cut(s) 988
HindIII AAGCTT 1 cut(s) 501
HinfI GANTC 2 cut(s) 548, 922
HphI GGTGA 2 cut(s) 475, 1025
Hpy166II GTNNAC 1 cut(s) 988
Hpy188I TCNGA 3 cut(s) 132, 679, 814
Hpy188III TCNNGA 5 cut(s) 119, 212, 350, 482, 803
Hpy8I GTNNAC 1 cut(s) 988
Hpy99I CGWCG 2 cut(s) 534, 1055
HpyAV CCTTC 4 cut(s) 68, 647, 956, 965
HpyCH4III ACNGT 4 cut(s) 164, 208, 233, 596
HpyCH4IV ACGT 4 cut(s) 532, 791, 949, 1050
HpyF3I CTNAG 3 cut(s) 9, 129, 327
HpySE526I ACGT 4 cut(s) 532, 791, 949, 1050
Hsp92II CATG 6 cut(s) 412, 524, 562, 584, 610, 940
Kzo9I GATC 1 cut(s) 562
LmnI GCTCC 4 cut(s) 316, 484, 805, 1006
Lsp1109I GCAGC 4 cut(s) 19, 809, 871, 926
LweI GCATC 3 cut(s) 247, 1006, 1051
MaeI CTAG 2 cut(s) 341, 552
MaeII ACGT 4 cut(s) 532, 791, 949, 1050
MaeIII GTNAC 2 cut(s) 332, 662
MalI GATC 1 cut(s) 564
MboI GATC 1 cut(s) 562
MboII GAAGA 7 cut(s) 92, 567, 848, 917, 920, 989, 1091
MflI RGATCY 1 cut(s) 562
MhlI GDGCHC 1 cut(s) 520
MluCI AATT 6 cut(s) 87, 188, 453, 485, 702, 1027
MlyI GAGTC 1 cut(s) 931
MseI TTAA 5 cut(s) 90, 443, 456, 890, 1046
MspA1I CMGCKG 2 cut(s) 50, 433
MspR9I CCNGG 1 cut(s) 857
MvaI CCWGG 1 cut(s) 857
NcoI CCATGG 1 cut(s) 558
NdeII GATC 1 cut(s) 562
NheI GCTAGC 1 cut(s) 340
NlaIII CATG 6 cut(s) 412, 524, 562, 584, 610, 940
NlaIV GGNNCC 2 cut(s) 564, 1002
NmuCI GTSAC 1 cut(s) 332
NspI RCATGY 2 cut(s) 412, 524
PaqCI CACCTGC 1 cut(s) 1001
PcsI WCGNNNNNNNCGW 1 cut(s) 1056
PfeI GAWTC 1 cut(s) 548
PfoI TCCNGGA 1 cut(s) 855
PkrI GCNGC 5 cut(s) 34, 43, 799, 886, 916
PleI GAGTC 1 cut(s) 930
PpsI GAGTC 1 cut(s) 930
PshBI ATTAAT 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 855
PspFI CCCAGC 1 cut(s) 586
PspGI CCWGG 1 cut(s) 855
PspN4I GGNNCC 2 cut(s) 564, 1002
PstI CTGCAG 2 cut(s) 37, 1072
PstNI CAGNNNCTG 1 cut(s) 683
PsuI RGATCY 1 cut(s) 562
PvuII CAGCTG 2 cut(s) 50, 433
RsaI GTAC 4 cut(s) 152, 290, 429, 593
RsaNI GTAC 4 cut(s) 151, 289, 428, 592
SaqAI TTAA 5 cut(s) 90, 443, 456, 890, 1046
SatI GCNGC 5 cut(s) 33, 42, 798, 885, 915
Sau3AI GATC 1 cut(s) 562
ScaI AGTACT 1 cut(s) 290
SchI GAGTC 1 cut(s) 931
ScrFI CCNGG 1 cut(s) 857
SduI GDGCHC 1 cut(s) 520
SfaNI GCATC 3 cut(s) 247, 1006, 1051
SfcI CTRYAG 2 cut(s) 33, 1068
SmlI CTYRAG 1 cut(s) 348
SmoI CTYRAG 1 cut(s) 348
Sse9I AATT 6 cut(s) 87, 188, 453, 485, 702, 1027
SsiI CCGC 1 cut(s) 41
SspMI CTAG 2 cut(s) 341, 552
StyD4I CCNGG 1 cut(s) 855
StyI CCWWGG 2 cut(s) 558, 713
TaaI ACNGT 4 cut(s) 164, 208, 233, 596
TaiI ACGT 4 cut(s) 535, 794, 952, 1053
TaqI TCGA 1 cut(s) 1053
TasI AATT 6 cut(s) 87, 188, 453, 485, 702, 1027
TatI WGTACW 1 cut(s) 288
TauI GCSGC 1 cut(s) 44
TfiI GAWTC 1 cut(s) 548
Tru1I TTAA 5 cut(s) 90, 443, 456, 890, 1046
Tru9I TTAA 5 cut(s) 90, 443, 456, 890, 1046
TscAI CASTG 2 cut(s) 267, 302
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 4 cut(s) 32, 797, 884, 914
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 8 cut(s) 201, 666, 749, 953, 971, 975, 984, 1092
TspRI CASTG 2 cut(s) 267, 302
VspI ATTAAT 1 cut(s) 456
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 2 cut(s) 485, 702
XceI RCATGY 2 cut(s) 412, 524
XspI CTAG 2 cut(s) 341, 552
ZrmI AGTACT 1 cut(s) 290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.