AT4G20810

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001364
Physical Location & Seq
Reverse (-)
11141427 .. 11143597
2171 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20810.1

Sequence Viewer

Length: 1251 bp
ATGGTGAAGCTTGTAGCGAAAACTTTCTATGATAATTATACTCCGAAAAACAATAATCAGAAGAAATCTGCAAAAAATGGCAGCGGAGGAATTGCCGTTTTGGTGCTGGACGCGCTTACAAGGCGGCAATGGGTTCGAGAAGAAGACTTGGCAAAAGAGTTGAAGTTAAACACCAAGCAACTTCGTACGATATTAAGATACTTTGAAGAACAACAATTTATCATGCGTGTTCACAGGAAAGAGAAGTCGAGTGCTACAACTAACGGCCGAGGAGAGGACAAAGTTAAGGTCCACATGTACTCGTATTGCTGTCTTGATTATTCACAGATATATGATGTTATTCGTTACAAACTGCACCGGATGAAGAAAGAATTCAAAGATGTACTAGAAGATAAAGATAACGTTCAGGAGTATGGCTGCCCTAACTGCAAAAGGAAGATTTTTTTTCATTGTGAAAATTGCAATGGTGAACTTGTTATGGAATGTAACAAGCTAACTTCCGAAGAAGTAGTAGTAGATGGAAGTGACAATCCAAGGAGTCGGCGGGATCATCTAAAAGATTTGCTTCAGAATATGGAGGTTCGACTGAAACCTCTGATGGATCATATAAACAGAATAAAAGACCTACCGGTTCCTAGCTTTGAATCTTTTCCGGCATGGGAGACTCGTGTAGCTAAGGCTGCTCGTGAAAATGGTGACCTTAACCCTGATGATACTTTGAGGCCACAGGGTGGGTATGGTTCAACACCGATGCCATTTCTCGGAGAGACAGAGATTGAGGTTAACCTTGGTGAAGAAAACGAAGATGTCAAATCTGATGAAGTTGGAGATTCTTCTAGAAGGAAACTCACGCCTTCATGGTTGATCAAGAAAGGAATGAATCTGTCCGATGAACAAAGAGGAGAAATAAGACATGAAGCAAAGGCTGATGATGGTGGCTCATCTATGGAGAATGGTGATGATGATAGGAATTTGAAGGATGAGTATCTCAAAGCTTACTACGCGGCTATACTGGAGGAGCAAGAGCTAGCTGAAAAGCTGAATCAGCAAGAATCCGCAGGCAAAGTAACAACTGACATTGAATTGGCTACTTCATCCTCAGATCGTCAGGTTGGTATGAAGTCCAAACGTGAAGAGGAAGAAGAAGAAGAAGCTTCTGTTGCAGCAAATGGAAACTACAAGGTGGACTTGAATGTGGAAGCAGAAGAAGCAGAACAAGACGAAAACGATGTCGATTGGCAAGAATGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

416

Amino Acids

47.8

Weight (kDa)

4.99

Isoelectric Point (pI)

47.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 29 - 134 5e-13 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 731
AccII CGCG 2 cut(s) 113, 1004
AciI CCGC 5 cut(s) 84, 124, 544, 1004, 1056
AclI AACGTT 1 cut(s) 402
AclWI GGATC 2 cut(s) 555, 609
AcoI YGGCCR 1 cut(s) 265
AcsI RAATTY 2 cut(s) 371, 970
AcuI CTGAAG 1 cut(s) 551
AdeI CACNNNGTG 1 cut(s) 731
AfaI GTAC 3 cut(s) 187, 299, 384
AfiI CCNNNNNNNGG 3 cut(s) 274, 731, 761
AflIII ACRYGT 1 cut(s) 294
AgeI ACCGGT 1 cut(s) 628
AgsI TTSAA 8 cut(s) 163, 206, 376, 644, 744, 976, 1082, 1192
AluBI AGCT 9 cut(s) 10, 493, 639, 674, 995, 1027, 1031, 1039, 1154
AluI AGCT 9 cut(s) 10, 493, 639, 674, 995, 1027, 1031, 1039, 1154
Alw26I GTCTC 2 cut(s) 656, 761
AlwI GGATC 2 cut(s) 555, 609
AoxI GGCC 2 cut(s) 265, 722
ApeKI GCWGC 4 cut(s) 81, 417, 680, 1163
ApoI RAATTY 2 cut(s) 371, 970
ArsI GACNNNNNNTTYG 4 cut(s) 273, 305, 1215, 1247
AsiGI ACCGGT 1 cut(s) 628
Asp700I GAANNNNTTC 3 cut(s) 23, 371, 648
AspLEI GCGC 1 cut(s) 115
AspS9I GGNCC 1 cut(s) 289
AsuHPI GGTGA 5 cut(s) 16, 479, 707, 803, 968
AsuNHI GCTAGC 1 cut(s) 1027
AvaII GGWCC 1 cut(s) 289
BauI CACGAG 2 cut(s) 666, 684
BbsI GAAGAC 1 cut(s) 150
BbvI GCAGC 4 cut(s) 93, 404, 667, 1175
BccI CCATC 3 cut(s) 512, 592, 926
BceAI ACGGC 2 cut(s) 80, 280
BcgI CGANNNNNNTGC 2 cut(s) 116, 150
BclI TGATCA 1 cut(s) 864
BcoDI GTCTC 2 cut(s) 656, 761
BfaI CTAG 4 cut(s) 386, 636, 837, 1028
BisI GCNGC 6 cut(s) 82, 125, 418, 681, 1005, 1164
BlsI GCNGC 6 cut(s) 83, 126, 419, 682, 1006, 1165
Bme18I GGWCC 1 cut(s) 289
BmgT120I GGNCC 1 cut(s) 289
BmiI GGNNCC 1 cut(s) 633
BmsI GCATC 1 cut(s) 741
BmtI GCTAGC 1 cut(s) 1031
BpiI GAAGAC 1 cut(s) 150
BpmI CTGGAG 1 cut(s) 1034
Bpu10I CCTNAGC 1 cut(s) 675
BsaBI GATNNNNATC 1 cut(s) 984
BsaJI CCNNGG 3 cut(s) 268, 533, 787
BsaWI WCCGGW 2 cut(s) 357, 628
Bsc4I CCNNNNNNNGG 3 cut(s) 274, 731, 761
Bse118I RCCGGY 1 cut(s) 628
Bse1I ACTGG 1 cut(s) 1017
Bse3DI GCAATG 2 cut(s) 134, 469
Bse8I GATNNNNATC 1 cut(s) 984
BseDI CCNNGG 3 cut(s) 268, 533, 787
BseGI GGATG 3 cut(s) 366, 985, 1094
BseJI GATNNNNATC 1 cut(s) 984
BseLI CCNNNNNNNGG 3 cut(s) 274, 731, 761
BseMI GCAATG 2 cut(s) 134, 469
BseMII CTCAG 1 cut(s) 1113
BseNI ACTGG 1 cut(s) 1017
BseRI GAGGAG 3 cut(s) 285, 915, 1031
BseX3I CGGCCG 1 cut(s) 265
BseXI GCAGC 4 cut(s) 93, 404, 667, 1175
BsgI GTGCAG 1 cut(s) 338
Bsh1236I CGCG 2 cut(s) 113, 1004
Bsh1285I CGRYCG 1 cut(s) 268
BshFI GGCC 2 cut(s) 267, 724
BshTI ACCGGT 1 cut(s) 628
BsiEI CGRYCG 1 cut(s) 268
BsiSI CCGG 3 cut(s) 358, 629, 653
BsiWI CGTACG 1 cut(s) 185
BslI CCNNNNNNNGG 3 cut(s) 274, 731, 761
BsmAI GTCTC 2 cut(s) 656, 761
BsmI GAATGC 1 cut(s) 1250
BsnI GGCC 2 cut(s) 267, 724
Bsp143I GATC 4 cut(s) 547, 601, 864, 1102
BspACI CCGC 5 cut(s) 84, 124, 544, 1004, 1056
BspANI GGCC 2 cut(s) 267, 724
BspCNI CTCAG 1 cut(s) 1112
BspFNI CGCG 2 cut(s) 113, 1004
BspLI GGNNCC 1 cut(s) 633
BspOI GCTAGC 1 cut(s) 1031
BspPI GGATC 2 cut(s) 555, 609
BsrDI GCAATG 2 cut(s) 134, 469
BsrFI RCCGGY 1 cut(s) 628
BsrI ACTGG 1 cut(s) 1017
BssAI RCCGGY 1 cut(s) 628
BssECI CCNNGG 3 cut(s) 268, 533, 787
BssMI GATC 4 cut(s) 547, 601, 864, 1102
BssSI CACGAG 2 cut(s) 666, 684
BssT1I CCWWGG 2 cut(s) 533, 787
Bst2BI CACGAG 2 cut(s) 666, 684
Bst6I CTCTTC 1 cut(s) 1128
BstC8I GCNNGC 2 cut(s) 1029, 1060
BstDEI CTNAG 2 cut(s) 675, 1099
BstEII GGTNACC 1 cut(s) 695
BstF5I GGATG 3 cut(s) 366, 985, 1094
BstFNI CGCG 2 cut(s) 113, 1004
BstHHI GCGC 1 cut(s) 115
BstKTI GATC 4 cut(s) 550, 604, 867, 1105
BstMAI GTCTC 2 cut(s) 656, 761
BstMBI GATC 4 cut(s) 547, 601, 864, 1102
BstMCI CGRYCG 1 cut(s) 268
BstMWI GCNNNNNNNGC 8 cut(s) 112, 121, 426, 680, 1001, 1045, 1160, 1208
BstNSI RCATGY 1 cut(s) 298
BstPI GGTNACC 1 cut(s) 695
BstUI CGCG 2 cut(s) 113, 1004
BstV1I GCAGC 4 cut(s) 93, 404, 667, 1175
BstV2I GAAGAC 1 cut(s) 150
BstZI CGGCCG 1 cut(s) 265
BsuRI GGCC 2 cut(s) 267, 724
BtsCI GGATG 3 cut(s) 366, 985, 1094
Cac8I GCNNGC 2 cut(s) 1029, 1060
CfoI GCGC 1 cut(s) 115
Cfr10I RCCGGY 1 cut(s) 628
Cfr13I GGNCC 1 cut(s) 289
CseI GACGC 1 cut(s) 119
Csp6I GTAC 3 cut(s) 186, 298, 383
CspAI ACCGGT 1 cut(s) 628
CviAII CATG 5 cut(s) 223, 295, 657, 858, 914
CviQI GTAC 3 cut(s) 186, 298, 383
DdeI CTNAG 2 cut(s) 675, 1099
DpnI GATC 4 cut(s) 549, 603, 866, 1104
DpnII GATC 4 cut(s) 547, 601, 864, 1102
DraIII CACNNNGTG 1 cut(s) 731
EaeI YGGCCR 1 cut(s) 265
EagI CGGCCG 1 cut(s) 265
Eam1104I CTCTTC 1 cut(s) 1128
EarI CTCTTC 1 cut(s) 1128
EclXI CGGCCG 1 cut(s) 265
Eco130I CCWWGG 2 cut(s) 533, 787
Eco47I GGWCC 1 cut(s) 289
Eco52I CGGCCG 1 cut(s) 265
Eco57I CTGAAG 1 cut(s) 551
Eco91I GGTNACC 1 cut(s) 695
EcoO65I GGTNACC 1 cut(s) 695
EcoRI GAATTC 1 cut(s) 371
EcoT14I CCWWGG 2 cut(s) 533, 787
ErhI CCWWGG 2 cut(s) 533, 787
FaeI CATG 5 cut(s) 226, 298, 660, 861, 917
FalI AAGNNNNNCTT 4 cut(s) 549, 581, 1172, 1204
FatI CATG 5 cut(s) 222, 294, 656, 857, 913
FauI CCCGC 1 cut(s) 537
FbaI TGATCA 1 cut(s) 864
Fnu4HI GCNGC 6 cut(s) 82, 125, 418, 681, 1005, 1164
FokI GGATG 3 cut(s) 373, 992, 1081
Fsp4HI GCNGC 6 cut(s) 82, 125, 418, 681, 1005, 1164
FspBI CTAG 4 cut(s) 386, 636, 837, 1028
GlaI GCGC 1 cut(s) 114
GluI GCNGC 6 cut(s) 82, 125, 418, 681, 1005, 1164
GsuI CTGGAG 1 cut(s) 1034
HaeIII GGCC 2 cut(s) 267, 724
HapII CCGG 3 cut(s) 358, 629, 653
HgaI GACGC 1 cut(s) 119
HhaI GCGC 1 cut(s) 115
Hin1II CATG 5 cut(s) 226, 298, 660, 861, 917
Hin6I GCGC 1 cut(s) 113
HinP1I GCGC 1 cut(s) 113
HincII GTYRAC 1 cut(s) 784
HindII GTYRAC 1 cut(s) 784
HindIII AAGCTT 3 cut(s) 8, 993, 1152
HinfI GANTC 7 cut(s) 538, 644, 664, 830, 880, 1042, 1052
HpaI GTTAAC 1 cut(s) 784
HpaII CCGG 3 cut(s) 358, 629, 653
HphI GGTGA 5 cut(s) 16, 479, 707, 803, 968
Hpy166II GTNNAC 5 cut(s) 232, 292, 470, 784, 1186
Hpy188I TCNGA 9 cut(s) 45, 60, 502, 570, 597, 764, 817, 889, 1102
Hpy188III TCNNGA 6 cut(s) 137, 314, 407, 686, 837, 868
Hpy8I GTNNAC 5 cut(s) 232, 292, 470, 784, 1186
HpyAV CCTTC 3 cut(s) 834, 864, 970
HpyCH4IV ACGT 2 cut(s) 402, 1129
HpyCH4V TGCA 5 cut(s) 71, 355, 429, 462, 1163
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 121, 426, 680, 1001, 1045, 1160, 1208
HpyF3I CTNAG 2 cut(s) 675, 1099
HpySE526I ACGT 2 cut(s) 402, 1129
Hsp92II CATG 5 cut(s) 226, 298, 660, 861, 917
HspAI GCGC 1 cut(s) 113
Ksp22I TGATCA 1 cut(s) 864
KspAI GTTAAC 1 cut(s) 784
Kzo9I GATC 4 cut(s) 547, 601, 864, 1102
LmnI GCTCC 1 cut(s) 1018
Lsp1109I GCAGC 4 cut(s) 93, 404, 667, 1175
LweI GCATC 1 cut(s) 741
MaeI CTAG 4 cut(s) 386, 636, 837, 1028
MaeII ACGT 2 cut(s) 402, 1129
MaeIII GTNAC 5 cut(s) 344, 485, 524, 695, 1066
MalI GATC 4 cut(s) 549, 603, 866, 1104
MboI GATC 4 cut(s) 547, 601, 864, 1102
MluCI AATT 7 cut(s) 34, 90, 215, 371, 457, 970, 1082
MlyI GAGTC 2 cut(s) 547, 658
MmeI TCCRAC 1 cut(s) 805
MroXI GAANNNNTTC 3 cut(s) 23, 371, 648
MseI TTAA 5 cut(s) 167, 194, 285, 702, 783
MspA1I CMGCKG 1 cut(s) 84
MspI CCGG 3 cut(s) 358, 629, 653
Mva1269I GAATGC 1 cut(s) 1250
MvnI CGCG 2 cut(s) 113, 1004
MwoI GCNNNNNNNGC 8 cut(s) 112, 121, 426, 680, 1001, 1045, 1160, 1208
NdeII GATC 4 cut(s) 547, 601, 864, 1102
NheI GCTAGC 1 cut(s) 1027
NlaIII CATG 5 cut(s) 226, 298, 660, 861, 917
NlaIV GGNNCC 1 cut(s) 633
NmeAIII GCCGAG 1 cut(s) 293
NmuCI GTSAC 2 cut(s) 524, 695
NspI RCATGY 1 cut(s) 298
PciI ACATGT 1 cut(s) 294
PctI GAATGC 1 cut(s) 1250
PdmI GAANNNNTTC 3 cut(s) 23, 371, 648
PfeI GAWTC 5 cut(s) 644, 830, 880, 1042, 1052
Pfl23II CGTACG 1 cut(s) 185
PflMI CCANNNNNTGG 1 cut(s) 731
PinAI ACCGGT 1 cut(s) 628
PkrI GCNGC 6 cut(s) 83, 126, 419, 682, 1006, 1165
PleI GAGTC 2 cut(s) 546, 658
PpsI GAGTC 2 cut(s) 546, 658
PscI ACATGT 1 cut(s) 294
Psp1406I AACGTT 1 cut(s) 402
PspEI GGTNACC 1 cut(s) 695
PspLI CGTACG 1 cut(s) 185
PspN4I GGNNCC 1 cut(s) 633
PspPI GGNCC 1 cut(s) 289
RsaI GTAC 3 cut(s) 187, 299, 384
RsaNI GTAC 3 cut(s) 186, 298, 383
SaqAI TTAA 5 cut(s) 167, 194, 285, 702, 783
SatI GCNGC 6 cut(s) 82, 125, 418, 681, 1005, 1164
Sau3AI GATC 4 cut(s) 547, 601, 864, 1102
Sau96I GGNCC 1 cut(s) 289
SchI GAGTC 2 cut(s) 547, 658
SfaNI GCATC 1 cut(s) 741
SinI GGWCC 1 cut(s) 289
Sse9I AATT 7 cut(s) 34, 90, 215, 371, 457, 970, 1082
SsiI CCGC 5 cut(s) 84, 124, 544, 1004, 1056
SspMI CTAG 4 cut(s) 386, 636, 837, 1028
StyI CCWWGG 2 cut(s) 533, 787
TaiI ACGT 2 cut(s) 405, 1132
TaqI TCGA 4 cut(s) 136, 248, 583, 1233
TasI AATT 7 cut(s) 34, 90, 215, 371, 457, 970, 1082
TatI WGTACW 2 cut(s) 297, 382
TauI GCSGC 2 cut(s) 127, 1007
TfiI GAWTC 5 cut(s) 644, 830, 880, 1042, 1052
Tru1I TTAA 5 cut(s) 167, 194, 285, 702, 783
Tru9I TTAA 5 cut(s) 167, 194, 285, 702, 783
TseFI GTSAC 2 cut(s) 524, 695
TseI GCWGC 4 cut(s) 81, 417, 680, 1163
Tsp45I GTSAC 2 cut(s) 524, 695
TspDTI ATGAA 9 cut(s) 377, 437, 834, 846, 893, 906, 930, 1083, 1133
Van91I CCANNNNNTGG 1 cut(s) 731
VpaK11BI GGWCC 1 cut(s) 289
XapI RAATTY 2 cut(s) 371, 970
XbaI TCTAGA 1 cut(s) 836
XceI RCATGY 1 cut(s) 298
XmnI GAANNNNTTC 3 cut(s) 23, 371, 648
XspI CTAG 4 cut(s) 386, 636, 837, 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.