Rh7DG068000

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0001364
Physical Location & Seq
Reverse (-)
5217989 .. 5225148
7160 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG068000.1

Sequence Viewer

Length: 1146 bp
ATGTATATCTATTCCTTACATCGCTTGATCTTTTTTTTGCAGACAGCTAAGGGTGTGAAGATTTTCAGTGCTGCGGTTGCTGCCACAGCCAATGGGCAACAACACAGAAAAGAAGGCGAAGAGAAAATTAAGCTGCAGACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCGTATGAGGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAATGCTTTGGATGCACTGCAACTCATCTCTTTGGAGGATGAGTACTTCCACTGCGAGAGTTGCAATGGGGAGCTTGTGGCTGAGAGTGACAAGCTAGCTTCTCAAGATGTAGGAGATGGAGATAGCAATGCAAGGAGGCAAAGGCGTGAAAAACTGAAAGACATGCTTCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGGGTGAAAGACTTACCTGCTCCTGAATTTGGAACTCTCCAAGCTTGGGAAGCACGAGCACATGCTCAACGACGTGCAGAGAATGGAGAATCTAGTGCCATGGATCCCTCCAAATCTTCACATGGGCTGGGGTACGGTGGAACGCCCATGCCTTTTCTTGGAGATACAAAAGTTGAAGTTGATTTTGGGAATGAAGGCAAGGGTAACGATGTCAAATCAGAAGCTGATATTACAGGCCAGAAAATTTTGCCACCTTGGATGATAAAGCAAGGGATGAACCTGACAAAGGAACAGCGTGGAGAGGTAAAGCAGGAGGCAAAGATGGATGGCACGTTAGCAGCTCCAGAGTATTCAGATGACAAAAAGTCAAATGCTGAAGATAATGATAAGGCTTTCCAGGATGAGTATTTCAAAGCCTATTACGCTGCTTTAATAAAAAGACAAGAAGAAGAAGCAGCAAAGAGTCGTCAGGTTGGCATGAAGTCAAAACGTGATCTGAATGAAGGAGATGAAGGAGAAGATAATGTTGACTGGCAAGAGGCTCCAGTTGCAGGTGATGCCAATGGAAATTACAAGGTCAATGACTTAAACGTCGAAGCCGATGCTACTGCAGAGGATGAAGATGACATAGACTGGGAGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

381

Amino Acids

43.09

Weight (kDa)

4.9

Isoelectric Point (pI)

38.86

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1040
AasI GACNNNNNNGTC 1 cut(s) 1088
Acc36I ACCTGC 2 cut(s) 523, 1040
AciI CCGC 1 cut(s) 74
AclWI GGATC 2 cut(s) 596, 609
AcsI RAATTY 2 cut(s) 524, 741
AcuI CTGAAG 2 cut(s) 494, 894
AfaI GTAC 4 cut(s) 191, 329, 468, 632
AfiI CCNNNNNNNGG 6 cut(s) 272, 527, 544, 656, 784, 1049
AgsI TTSAA 3 cut(s) 455, 674, 910
AhdI GACNNNNNGTC 1 cut(s) 862
AjiI CACGTC 1 cut(s) 572
AjnI CCWGG 1 cut(s) 894
AjuI GAANNNNNNNTTGG 4 cut(s) 212, 244, 666, 698
AluBI AGCT 9 cut(s) 47, 133, 358, 379, 383, 472, 542, 722, 839
AluI AGCT 9 cut(s) 47, 133, 358, 379, 383, 472, 542, 722, 839
Alw21I GWGCWC 1 cut(s) 559
AlwI GGATC 2 cut(s) 596, 609
AlwNI CAGNNNCTG 1 cut(s) 722
AoxI GGCC 1 cut(s) 733
ApeKI GCWGC 6 cut(s) 71, 80, 133, 836, 923, 953
ApoI RAATTY 2 cut(s) 524, 741
AseI ATTAAT 1 cut(s) 495
Asp700I GAANNNNTTC 1 cut(s) 62
AsuHPI GGTGA 2 cut(s) 514, 1064
AsuNHI GCTAGC 1 cut(s) 379
BaeI ACNNNNGTAYC 2 cut(s) 654, 687
BamHI GGATCC 1 cut(s) 601
BauI CACGAG 1 cut(s) 552
Bbv12I GWGCWC 1 cut(s) 559
BbvI GCAGC 6 cut(s) 58, 67, 120, 848, 910, 965
BccI CCATC 3 cut(s) 395, 814, 818
BcgI CGANNNNNNTGC 4 cut(s) 1082, 1088, 1116, 1122
BciT130I CCWGG 1 cut(s) 896
BfaI CTAG 2 cut(s) 380, 591
BfmI CTRYAG 2 cut(s) 134, 1107
BfuAI ACCTGC 2 cut(s) 523, 1040
BisI GCNGC 6 cut(s) 72, 81, 134, 837, 924, 954
BlsI GCNGC 6 cut(s) 73, 82, 135, 838, 925, 955
BmcAI AGTACT 1 cut(s) 329
Bme1390I CCNGG 1 cut(s) 896
BmeRI GACNNNNNGTC 1 cut(s) 862
BmgBI CACGTC 1 cut(s) 572
BmiI GGNNCC 2 cut(s) 603, 1041
BmrFI CCNGG 1 cut(s) 896
BmrI ACTGGG 1 cut(s) 1141
BmsI GCATC 3 cut(s) 286, 1045, 1090
BmtI GCTAGC 1 cut(s) 383
BmuI ACTGGG 1 cut(s) 1141
BpmI CTGGAG 2 cut(s) 825, 1026
Bpu10I CCTNAGC 1 cut(s) 48
BpuEI CTTGAG 1 cut(s) 372
BsaJI CCNNGG 2 cut(s) 597, 752
BsaXI ACNNNNNCTCC 2 cut(s) 311, 341
Bsc4I CCNNNNNNNGG 6 cut(s) 272, 527, 544, 656, 784, 1049
Bse1I ACTGG 3 cut(s) 1034, 1043, 1136
Bse3DI GCAATG 2 cut(s) 355, 418
BseBI CCWGG 1 cut(s) 896
BseDI CCNNGG 2 cut(s) 597, 752
BseGI GGATG 7 cut(s) 301, 328, 762, 777, 829, 904, 1120
BseLI CCNNNNNNNGG 6 cut(s) 272, 527, 544, 656, 784, 1049
BseMI GCAATG 2 cut(s) 355, 418
BseMII CTCAG 2 cut(s) 182, 357
BseNI ACTGG 3 cut(s) 1034, 1043, 1136
BseXI GCAGC 6 cut(s) 58, 67, 120, 848, 910, 965
BseYI CCCAGC 1 cut(s) 625
BsgI GTGCAG 2 cut(s) 182, 594
BshFI GGCC 1 cut(s) 735
BsiHKAI GWGCWC 1 cut(s) 559
BslI CCNNNNNNNGG 6 cut(s) 272, 527, 544, 656, 784, 1049
BsnI GGCC 1 cut(s) 735
Bsp1286I GDGCHC 1 cut(s) 559
Bsp143I GATC 3 cut(s) 27, 601, 991
Bsp19I CCATGG 1 cut(s) 597
BspACI CCGC 1 cut(s) 74
BspANI GGCC 1 cut(s) 735
BspCNI CTCAG 2 cut(s) 181, 358
BspLI GGNNCC 2 cut(s) 603, 1041
BspMAI CTGCAG 2 cut(s) 138, 1111
BspMI ACCTGC 2 cut(s) 523, 1040
BspOI GCTAGC 1 cut(s) 383
BspPI GGATC 2 cut(s) 596, 609
BsrDI GCAATG 2 cut(s) 355, 418
BsrI ACTGG 3 cut(s) 1034, 1043, 1136
BssECI CCNNGG 2 cut(s) 597, 752
BssMI GATC 3 cut(s) 27, 601, 991
BssSI CACGAG 1 cut(s) 552
BssT1I CCWWGG 2 cut(s) 597, 752
Bst2BI CACGAG 1 cut(s) 552
Bst2UI CCWGG 1 cut(s) 896
Bst4CI ACNGT 4 cut(s) 203, 247, 272, 635
Bst6I CTCTTC 1 cut(s) 114
BstAPI GCANNNNNTGC 1 cut(s) 1055
BstC8I GCNNGC 1 cut(s) 381
BstDEI CTNAG 3 cut(s) 48, 168, 366
BstDSI CCRYGG 1 cut(s) 597
BstENI CCTNNNNNAGG 1 cut(s) 782
BstF5I GGATG 7 cut(s) 301, 328, 762, 777, 829, 904, 1120
BstKTI GATC 3 cut(s) 30, 604, 994
BstMBI GATC 3 cut(s) 27, 601, 991
BstMWI GCNNNNNNNGC 9 cut(s) 77, 80, 86, 296, 345, 548, 920, 1046, 1055
BstNI CCWGG 1 cut(s) 896
BstNSI RCATGY 2 cut(s) 451, 563
BstSCI CCNGG 1 cut(s) 894
BstSFI CTRYAG 2 cut(s) 134, 1107
BstV1I GCAGC 6 cut(s) 58, 67, 120, 848, 910, 965
BstX2I RGATCY 1 cut(s) 601
BstYI RGATCY 1 cut(s) 601
BsuRI GGCC 1 cut(s) 735
BtgI CCRYGG 1 cut(s) 597
BtgZI GCGATG 1 cut(s) 5
BtrI CACGTC 1 cut(s) 572
BtsCI GGATG 7 cut(s) 301, 328, 762, 777, 829, 904, 1120
BtsI GCAGTG 2 cut(s) 299, 334
BtsIMutI CAGTG 3 cut(s) 73, 299, 334
BveI ACCTGC 2 cut(s) 523, 1040
Cac8I GCNNGC 1 cut(s) 381
CaiI CAGNNNCTG 1 cut(s) 722
Csp6I GTAC 4 cut(s) 190, 328, 467, 631
CviAII CATG 6 cut(s) 448, 560, 598, 620, 646, 976
CviQI GTAC 4 cut(s) 190, 328, 467, 631
DdeI CTNAG 3 cut(s) 48, 168, 366
DpnI GATC 3 cut(s) 29, 603, 993
DpnII GATC 3 cut(s) 27, 601, 991
DrdI GACNNNNNNGTC 1 cut(s) 1088
DriI GACNNNNNGTC 1 cut(s) 862
DseDI GACNNNNNNGTC 1 cut(s) 1088
Eam1104I CTCTTC 1 cut(s) 114
Eam1105I GACNNNNNGTC 1 cut(s) 862
EarI CTCTTC 1 cut(s) 114
Eco130I CCWWGG 2 cut(s) 597, 752
Eco57I CTGAAG 2 cut(s) 494, 894
EcoNI CCTNNNNNAGG 1 cut(s) 782
EcoRII CCWGG 1 cut(s) 894
EcoT14I CCWWGG 2 cut(s) 597, 752
ErhI CCWWGG 2 cut(s) 597, 752
FaeI CATG 6 cut(s) 451, 563, 601, 623, 649, 979
FalI AAGNNNNNCTT 2 cut(s) 435, 467
FatI CATG 6 cut(s) 447, 559, 597, 619, 645, 975
Fnu4HI GCNGC 6 cut(s) 72, 81, 134, 837, 924, 954
FokI GGATG 7 cut(s) 308, 335, 769, 784, 836, 911, 1127
Fsp4HI GCNGC 6 cut(s) 72, 81, 134, 837, 924, 954
FspBI CTAG 2 cut(s) 380, 591
GluI GCNGC 6 cut(s) 72, 81, 134, 837, 924, 954
GsaI CCCAGC 1 cut(s) 629
GsuI CTGGAG 2 cut(s) 825, 1026
HaeIII GGCC 1 cut(s) 735
Hin1II CATG 6 cut(s) 451, 563, 601, 623, 649, 979
HincII GTYRAC 1 cut(s) 1027
HindII GTYRAC 1 cut(s) 1027
HindIII AAGCTT 1 cut(s) 540
HinfI GANTC 3 cut(s) 139, 587, 961
HphI GGTGA 2 cut(s) 514, 1064
Hpy166II GTNNAC 1 cut(s) 1027
Hpy188I TCNGA 4 cut(s) 171, 718, 853, 996
Hpy188III TCNNGA 5 cut(s) 158, 251, 389, 521, 842
Hpy8I GTNNAC 1 cut(s) 1027
Hpy99I CGWCG 2 cut(s) 573, 1094
HpyAV CCTTC 4 cut(s) 107, 686, 995, 1004
HpyCH4III ACNGT 4 cut(s) 203, 247, 272, 635
HpyCH4IV ACGT 4 cut(s) 571, 830, 988, 1089
HpyF10VI GCNNNNNNNGC 9 cut(s) 77, 80, 86, 296, 345, 548, 920, 1046, 1055
HpyF3I CTNAG 3 cut(s) 48, 168, 366
HpySE526I ACGT 4 cut(s) 571, 830, 988, 1089
Hsp92II CATG 6 cut(s) 451, 563, 601, 623, 649, 979
Kzo9I GATC 3 cut(s) 27, 601, 991
LmnI GCTCC 4 cut(s) 355, 523, 844, 1045
Lsp1109I GCAGC 6 cut(s) 58, 67, 120, 848, 910, 965
LweI GCATC 3 cut(s) 286, 1045, 1090
MaeI CTAG 2 cut(s) 380, 591
MaeII ACGT 4 cut(s) 571, 830, 988, 1089
MaeIII GTNAC 2 cut(s) 371, 701
MalI GATC 3 cut(s) 29, 603, 993
MboI GATC 3 cut(s) 27, 601, 991
MboII GAAGA 8 cut(s) 70, 131, 606, 887, 956, 959, 1028, 1130
MflI RGATCY 1 cut(s) 601
MhlI GDGCHC 1 cut(s) 559
MluCI AATT 6 cut(s) 126, 227, 492, 524, 741, 1066
MlyI GAGTC 2 cut(s) 133, 970
MroXI GAANNNNTTC 1 cut(s) 62
MseI TTAA 5 cut(s) 129, 482, 495, 929, 1085
MspA1I CMGCKG 1 cut(s) 472
MspR9I CCNGG 1 cut(s) 896
MvaI CCWGG 1 cut(s) 896
MwoI GCNNNNNNNGC 9 cut(s) 77, 80, 86, 296, 345, 548, 920, 1046, 1055
NcoI CCATGG 1 cut(s) 597
NdeII GATC 3 cut(s) 27, 601, 991
NheI GCTAGC 1 cut(s) 379
NlaIII CATG 6 cut(s) 451, 563, 601, 623, 649, 979
NlaIV GGNNCC 2 cut(s) 603, 1041
NmuCI GTSAC 1 cut(s) 371
NspI RCATGY 2 cut(s) 451, 563
PaqCI CACCTGC 1 cut(s) 1040
PcsI WCGNNNNNNNCGW 1 cut(s) 1095
PdmI GAANNNNTTC 1 cut(s) 62
PfeI GAWTC 1 cut(s) 587
PfoI TCCNGGA 1 cut(s) 894
PkrI GCNGC 6 cut(s) 73, 82, 135, 838, 925, 955
PleI GAGTC 2 cut(s) 133, 969
PpsI GAGTC 2 cut(s) 133, 969
PshBI ATTAAT 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 894
PspFI CCCAGC 1 cut(s) 625
PspGI CCWGG 1 cut(s) 894
PspN4I GGNNCC 2 cut(s) 603, 1041
PstI CTGCAG 2 cut(s) 138, 1111
PstNI CAGNNNCTG 1 cut(s) 722
PsuI RGATCY 1 cut(s) 601
PvuII CAGCTG 1 cut(s) 472
RsaI GTAC 4 cut(s) 191, 329, 468, 632
RsaNI GTAC 4 cut(s) 190, 328, 467, 631
SaqAI TTAA 5 cut(s) 129, 482, 495, 929, 1085
SatI GCNGC 6 cut(s) 72, 81, 134, 837, 924, 954
Sau3AI GATC 3 cut(s) 27, 601, 991
ScaI AGTACT 1 cut(s) 329
SchI GAGTC 2 cut(s) 133, 970
ScrFI CCNGG 1 cut(s) 896
SduI GDGCHC 1 cut(s) 559
SfaNI GCATC 3 cut(s) 286, 1045, 1090
SfcI CTRYAG 2 cut(s) 134, 1107
SmlI CTYRAG 1 cut(s) 387
SmoI CTYRAG 1 cut(s) 387
Sse9I AATT 6 cut(s) 126, 227, 492, 524, 741, 1066
SsiI CCGC 1 cut(s) 74
SspMI CTAG 2 cut(s) 380, 591
StyD4I CCNGG 1 cut(s) 894
StyI CCWWGG 2 cut(s) 597, 752
TaaI ACNGT 4 cut(s) 203, 247, 272, 635
TaiI ACGT 4 cut(s) 574, 833, 991, 1092
TaqI TCGA 1 cut(s) 1092
TasI AATT 6 cut(s) 126, 227, 492, 524, 741, 1066
TatI WGTACW 1 cut(s) 327
TfiI GAWTC 1 cut(s) 587
Tru1I TTAA 5 cut(s) 129, 482, 495, 929, 1085
Tru9I TTAA 5 cut(s) 129, 482, 495, 929, 1085
TscAI CASTG 3 cut(s) 73, 306, 341
TseFI GTSAC 1 cut(s) 371
TseI GCWGC 6 cut(s) 71, 80, 133, 836, 923, 953
Tsp45I GTSAC 1 cut(s) 371
TspDTI ATGAA 7 cut(s) 240, 705, 788, 992, 1014, 1023, 1131
TspRI CASTG 3 cut(s) 73, 306, 341
VspI ATTAAT 1 cut(s) 495
XagI CCTNNNNNAGG 1 cut(s) 782
XapI RAATTY 2 cut(s) 524, 741
XceI RCATGY 2 cut(s) 451, 563
XmnI GAANNNNTTC 1 cut(s) 62
XspI CTAG 2 cut(s) 380, 591
ZrmI AGTACT 1 cut(s) 329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.