MD14G1221400.v1.1

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0001364
Physical Location & Seq
Reverse (-)
30310183 .. 30316052
5870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1221400.v1.1.491

Sequence Viewer

Length: 1413 bp
ATGAGCATCGAGCCGTTTAACAAGTTGGTGAAGCTTACAGCGAGGGCGTTCTACGATGACATACCGGCGAAAGGGGAGAATCAGCCCAAGACCGGCCGGAGCGACAATCGGGGGATTGCGGTGGTGGTGCTCGATGCGCTGTCGAGACGACAATGGGTAAGAGAAGAAGATTTGGCAAAGGACTTGAAACTGCACTCGAAGCAGCTTCGTCGTACTTTGCGATTCTTTGAGGAGGAAAAACTGGTCACCAGAGATCATAGGAGAGAGACAGCTAAGGGTGCGAAGATGCATAATGCTGCTGTCGCTGCCGCAGGAGATGGGCCACCTGTAAAAGAAGGGGAAGAGAAAGTTAAGATGCACACTCACTCTTACTGCTGTCTGGACTATGCACAGATATACGATGTAGTAAGGTACAGACTGCACCGTTTGCGGAAAAAATTGAAAGATGAGTTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAACGCTTTAGATGCTCTGCAACTGGTTTCTATGGAGGATGAGTACTTCCATTGTGAAAATTGCAATGGAGAACTTGTGGCTGAGAGTGACAAACTAGCTTCGCAAGAAGTGGTAGGAGATGGAGATGACAATGCAAGGAGGCGGCGGCGTGAAAAATTAAAGGACTTGCTTCAAAAGATGGAGGTACAGCTGAAGCCTCTAATGGAACAAATTAATAGAGTTAAAGACTTATCTCCTCCTGAATTTGGAAGTCTCCAAGCTTGGGAAGCGCGAGCAAGTGCTGCCAGACGTGCAGAGAACGGTGAGTCTAGTGCCATGGATCCCTCCAAATCTTCGCATGGGATGGGATATGGTGGAACGCCAATGCCCTTTCTTGGAGATACAAAGGTTGAAGTTGATTTCTCTGGGGTTGAAGGAAAGGGCAAGGATATAAAATCTGAAGCTGATGGTACAAGCCTGAAAGTTTTGCCACCTTGGATGATCAAGCAAGGCATGAACCTTACGAAAGAACAACGTGGAGAGGTCAAGCAGGAGCAAAAGATGGATGGCAGTGGAAGTGTAGCAGCTCCAGAGTTCTCAGAAGACAAAAAATTCAGTACTGAAAATAATGAGAAAAATATACAGGATGAGTATTTAAAAGCATATTATGCTGCTCTAATAAAAAGACAGCAAGAAGAAGAAGCAAAGAAAGAACAGGAAACACACATCTCTGATGGTCCCTCTGCAGTGTCTCCTAATCGTCAGGTTGGCATGAAGTCAAAGCGCGAGGATGAAGGAGAAGATAACGTAGACTGGGAAGAGGCTCTAGTTACAGGTAATGCAGAAAATTATAAGGTCCATGACTTGAACGTTGAGGCTGATGCTACAGCAGACGATGAGGACGATATAGACTGGGAGGAAGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

471

Amino Acids

53.19

Weight (kDa)

5.25

Isoelectric Point (pI)

54.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 40 - 93 4.1e-06 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1338
AccI GTMKAC 1 cut(s) 1296
AccII CGCG 2 cut(s) 778, 1272
AciI CCGC 5 cut(s) 119, 309, 430, 649, 652
AclI AACGTT 1 cut(s) 1356
AclWI GGATC 2 cut(s) 821, 834
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 749, 1097
AcuI CTGAAG 2 cut(s) 719, 966
AfaI GTAC 6 cut(s) 214, 413, 551, 693, 958, 1105
AfiI CCNNNNNNNGG 6 cut(s) 71, 92, 494, 752, 769, 881
AgsI TTSAA 6 cut(s) 187, 442, 680, 899, 920, 1354
AjiI CACGTC 1 cut(s) 797
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
AluBI AGCT 8 cut(s) 34, 205, 272, 605, 697, 767, 950, 1073
AluI AGCT 8 cut(s) 34, 205, 272, 605, 697, 767, 950, 1073
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 4 cut(s) 139, 260, 764, 1242
AlwI GGATC 2 cut(s) 821, 834
AoxI GGCC 2 cut(s) 94, 320
ApeKI GCWGC 6 cut(s) 202, 296, 305, 788, 1070, 1157
ApoI RAATTY 2 cut(s) 749, 1097
AseI ATTAAT 1 cut(s) 720
AspLEI GCGC 3 cut(s) 139, 778, 1272
AspS9I GGNCC 3 cut(s) 320, 1223, 1342
AsuHPI GGTGA 3 cut(s) 40, 238, 821
AvaII GGWCC 2 cut(s) 1223, 1342
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BamHI GGATCC 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 6 cut(s) 214, 283, 292, 775, 1082, 1144
BccI CCATC 8 cut(s) 311, 620, 679, 844, 947, 1042, 1046, 1214
BcgI CGANNNNNNTGC 2 cut(s) 191, 225
BclI TGATCA 1 cut(s) 987
BcoDI GTCTC 4 cut(s) 139, 260, 764, 1242
BfaI CTAG 3 cut(s) 602, 816, 1313
BfmI CTRYAG 2 cut(s) 1230, 1371
BisI GCNGC 9 cut(s) 203, 297, 306, 309, 650, 653, 789, 1071, 1158
BlsI GCNGC 9 cut(s) 204, 298, 307, 310, 651, 654, 790, 1072, 1159
BmcAI AGTACT 2 cut(s) 551, 1105
Bme18I GGWCC 2 cut(s) 1223, 1342
BmgBI CACGTC 1 cut(s) 797
BmgT120I GGNCC 3 cut(s) 320, 1223, 1342
BmiI GGNNCC 2 cut(s) 828, 1225
BmrI ACTGGG 2 cut(s) 1309, 1408
BmsI GCATC 6 cut(s) 15, 124, 276, 345, 508, 1357
BmuI ACTGGG 2 cut(s) 1309, 1408
BpiI GAAGAC 1 cut(s) 1095
BpmI CTGGAG 1 cut(s) 1059
Bpu10I CCTNAGC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 822, 980
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 567, 597
Bsc4I CCNNNNNNNGG 6 cut(s) 71, 92, 494, 752, 769, 881
Bse118I RCCGGY 2 cut(s) 64, 92
Bse1I ACTGG 4 cut(s) 246, 534, 1304, 1403
Bse3DI GCAATG 1 cut(s) 577
BseDI CCNNGG 2 cut(s) 822, 980
BseGI GGATG 6 cut(s) 550, 855, 990, 1057, 1138, 1282
BseLI CCNNNNNNNGG 6 cut(s) 71, 92, 494, 752, 769, 881
BseMI GCAATG 1 cut(s) 577
BseMII CTCAG 2 cut(s) 579, 1098
BseNI ACTGG 4 cut(s) 246, 534, 1304, 1403
BseRI GAGGAG 2 cut(s) 245, 732
BseX3I CGGCCG 1 cut(s) 94
BseXI GCAGC 6 cut(s) 214, 283, 292, 775, 1082, 1144
BsgI GTGCAG 3 cut(s) 176, 404, 819
Bsh1236I CGCG 2 cut(s) 778, 1272
Bsh1285I CGRYCG 1 cut(s) 97
BshFI GGCC 2 cut(s) 96, 322
BsiEI CGRYCG 1 cut(s) 97
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 3 cut(s) 65, 93, 97
BslFI GGGAC 1 cut(s) 1209
BslI CCNNNNNNNGG 6 cut(s) 71, 92, 494, 752, 769, 881
BsmAI GTCTC 4 cut(s) 139, 260, 764, 1242
BsmBI CGTCTC 1 cut(s) 139
BsmFI GGGAC 1 cut(s) 1209
BsnI GGCC 2 cut(s) 96, 322
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 3 cut(s) 253, 826, 987
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 5 cut(s) 119, 309, 430, 649, 652
BspANI GGCC 2 cut(s) 96, 322
BspCNI CTCAG 2 cut(s) 580, 1097
BspFNI CGCG 2 cut(s) 778, 1272
BspLI GGNNCC 2 cut(s) 828, 1225
BspMAI CTGCAG 1 cut(s) 1234
BspPI GGATC 2 cut(s) 821, 834
BsrDI GCAATG 1 cut(s) 577
BsrFI RCCGGY 2 cut(s) 64, 92
BsrI ACTGG 4 cut(s) 246, 534, 1304, 1403
BssAI RCCGGY 2 cut(s) 64, 92
BssECI CCNNGG 2 cut(s) 822, 980
BssMI GATC 3 cut(s) 253, 826, 987
BssT1I CCWWGG 2 cut(s) 822, 980
Bst4CI ACNGT 4 cut(s) 425, 469, 494, 809
Bst6I CTCTTC 2 cut(s) 336, 1299
BstAPI GCANNNNNTGC 3 cut(s) 427, 788, 1154
BstC8I GCNNGC 1 cut(s) 780
BstDEI CTNAG 3 cut(s) 273, 588, 1084
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 6 cut(s) 550, 855, 990, 1057, 1138, 1282
BstFNI CGCG 2 cut(s) 778, 1272
BstHHI GCGC 3 cut(s) 139, 778, 1272
BstKTI GATC 3 cut(s) 256, 829, 990
BstMAI GTCTC 4 cut(s) 139, 260, 764, 1242
BstMBI GATC 3 cut(s) 253, 826, 987
BstMCI CGRYCG 1 cut(s) 97
BstPI GGTNACC 1 cut(s) 244
BstSFI CTRYAG 2 cut(s) 1230, 1371
BstUI CGCG 2 cut(s) 778, 1272
BstV1I GCAGC 6 cut(s) 214, 283, 292, 775, 1082, 1144
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 1 cut(s) 826
BstYI RGATCY 1 cut(s) 826
BstZI CGGCCG 1 cut(s) 94
BsuRI GGCC 2 cut(s) 96, 322
BtgI CCRYGG 1 cut(s) 822
BtrI CACGTC 1 cut(s) 797
BtsCI GGATG 6 cut(s) 550, 855, 990, 1057, 1138, 1282
BtsI GCAGTG 2 cut(s) 1063, 1239
BtsIMutI CAGTG 2 cut(s) 1063, 1239
Cac8I GCNNGC 1 cut(s) 780
CfoI GCGC 3 cut(s) 139, 778, 1272
Cfr10I RCCGGY 2 cut(s) 64, 92
Cfr13I GGNCC 3 cut(s) 320, 1223, 1342
Csp6I GTAC 6 cut(s) 213, 412, 550, 692, 957, 1104
CviAII CATG 5 cut(s) 823, 845, 1000, 1258, 1346
CviQI GTAC 6 cut(s) 213, 412, 550, 692, 957, 1104
DdeI CTNAG 3 cut(s) 273, 588, 1084
DpnI GATC 3 cut(s) 255, 828, 989
DpnII GATC 3 cut(s) 253, 826, 987
DraI TTTAAA 1 cut(s) 1143
EaeI YGGCCR 1 cut(s) 94
EagI CGGCCG 1 cut(s) 94
Eam1104I CTCTTC 2 cut(s) 336, 1299
EarI CTCTTC 2 cut(s) 336, 1299
EclXI CGGCCG 1 cut(s) 94
Eco130I CCWWGG 2 cut(s) 822, 980
Eco47I GGWCC 2 cut(s) 1223, 1342
Eco52I CGGCCG 1 cut(s) 94
Eco57I CTGAAG 2 cut(s) 719, 966
Eco91I GGTNACC 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 822, 980
EcoT22I ATGCAT 1 cut(s) 291
ErhI CCWWGG 2 cut(s) 822, 980
Esp3I CGTCTC 1 cut(s) 139
FaeI CATG 5 cut(s) 826, 848, 1003, 1261, 1349
FaqI GGGAC 1 cut(s) 1209
FatI CATG 5 cut(s) 822, 844, 999, 1257, 1345
FbaI TGATCA 1 cut(s) 987
FblI GTMKAC 1 cut(s) 1296
Fnu4HI GCNGC 9 cut(s) 203, 297, 306, 309, 650, 653, 789, 1071, 1158
FokI GGATG 6 cut(s) 557, 862, 997, 1064, 1145, 1289
Fsp4HI GCNGC 9 cut(s) 203, 297, 306, 309, 650, 653, 789, 1071, 1158
FspBI CTAG 3 cut(s) 602, 816, 1313
GlaI GCGC 3 cut(s) 138, 777, 1271
GluI GCNGC 9 cut(s) 203, 297, 306, 309, 650, 653, 789, 1071, 1158
GsuI CTGGAG 1 cut(s) 1059
HaeIII GGCC 2 cut(s) 96, 322
HapII CCGG 3 cut(s) 65, 93, 97
HhaI GCGC 3 cut(s) 139, 778, 1272
Hin1II CATG 5 cut(s) 826, 848, 1003, 1261, 1349
Hin6I GCGC 3 cut(s) 137, 776, 1270
HinP1I GCGC 3 cut(s) 137, 776, 1270
HindIII AAGCTT 2 cut(s) 32, 765
HinfI GANTC 3 cut(s) 79, 222, 812
HpaII CCGG 3 cut(s) 65, 93, 97
HphI GGTGA 3 cut(s) 40, 238, 821
Hpy166II GTNNAC 1 cut(s) 1297
Hpy188I TCNGA 3 cut(s) 946, 1087, 1219
Hpy188III TCNNGA 5 cut(s) 144, 380, 473, 746, 1076
Hpy8I GTNNAC 1 cut(s) 1297
Hpy99I CGWCG 1 cut(s) 213
HpyAV CCTTC 4 cut(s) 329, 914, 1274, 1400
HpyCH4III ACNGT 4 cut(s) 425, 469, 494, 809
HpyCH4IV ACGT 4 cut(s) 796, 1021, 1293, 1356
HpyF3I CTNAG 3 cut(s) 273, 588, 1084
HpySE526I ACGT 4 cut(s) 796, 1021, 1293, 1356
Hsp92II CATG 5 cut(s) 826, 848, 1003, 1261, 1349
HspAI GCGC 3 cut(s) 137, 776, 1270
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 3 cut(s) 253, 826, 987
LmnI GCTCC 3 cut(s) 99, 1039, 1078
Lsp1109I GCAGC 6 cut(s) 214, 283, 292, 775, 1082, 1144
LweI GCATC 6 cut(s) 15, 124, 276, 345, 508, 1357
MaeI CTAG 3 cut(s) 602, 816, 1313
MaeII ACGT 4 cut(s) 796, 1021, 1293, 1356
MaeIII GTNAC 3 cut(s) 244, 593, 1315
MalI GATC 3 cut(s) 255, 828, 989
MboI GATC 3 cut(s) 253, 826, 987
MflI RGATCY 1 cut(s) 826
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 7 cut(s) 437, 565, 662, 717, 749, 1097, 1333
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 1 cut(s) 432
Mph1103I ATGCAT 1 cut(s) 291
MseI TTAA 6 cut(s) 18, 351, 665, 720, 729, 1142
MspA1I CMGCKG 1 cut(s) 697
MspI CCGG 3 cut(s) 65, 93, 97
MvnI CGCG 2 cut(s) 778, 1272
NcoI CCATGG 1 cut(s) 822
NdeII GATC 3 cut(s) 253, 826, 987
NlaIII CATG 5 cut(s) 826, 848, 1003, 1261, 1349
NlaIV GGNNCC 2 cut(s) 828, 1225
NmuCI GTSAC 2 cut(s) 244, 593
NsiI ATGCAT 1 cut(s) 291
PcsI WCGNNNNNNNCGW 2 cut(s) 217, 1386
PfeI GAWTC 2 cut(s) 79, 222
PkrI GCNGC 9 cut(s) 204, 298, 307, 310, 651, 654, 790, 1072, 1159
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PshBI ATTAAT 1 cut(s) 720
PsiI TTATAA 1 cut(s) 1338
Psp1406I AACGTT 1 cut(s) 1356
PspEI GGTNACC 1 cut(s) 244
PspN4I GGNNCC 2 cut(s) 828, 1225
PspPI GGNCC 3 cut(s) 320, 1223, 1342
PstI CTGCAG 1 cut(s) 1234
PsuI RGATCY 1 cut(s) 826
PvuII CAGCTG 1 cut(s) 697
RsaI GTAC 6 cut(s) 214, 413, 551, 693, 958, 1105
RsaNI GTAC 6 cut(s) 213, 412, 550, 692, 957, 1104
SaqAI TTAA 6 cut(s) 18, 351, 665, 720, 729, 1142
SatI GCNGC 9 cut(s) 203, 297, 306, 309, 650, 653, 789, 1071, 1158
Sau3AI GATC 3 cut(s) 253, 826, 987
Sau96I GGNCC 3 cut(s) 320, 1223, 1342
ScaI AGTACT 2 cut(s) 551, 1105
SchI GAGTC 1 cut(s) 821
SduI GDGCHC 1 cut(s) 132
SfaNI GCATC 6 cut(s) 15, 124, 276, 345, 508, 1357
SfcI CTRYAG 2 cut(s) 1230, 1371
SinI GGWCC 2 cut(s) 1223, 1342
Sse9I AATT 7 cut(s) 437, 565, 662, 717, 749, 1097, 1333
SsiI CCGC 5 cut(s) 119, 309, 430, 649, 652
SspMI CTAG 3 cut(s) 602, 816, 1313
StyI CCWWGG 2 cut(s) 822, 980
TaaI ACNGT 4 cut(s) 425, 469, 494, 809
TaiI ACGT 4 cut(s) 799, 1024, 1296, 1359
TaqI TCGA 4 cut(s) 9, 132, 143, 197
TasI AATT 7 cut(s) 437, 565, 662, 717, 749, 1097, 1333
TatI WGTACW 2 cut(s) 549, 1103
TauI GCSGC 3 cut(s) 311, 652, 655
TfiI GAWTC 2 cut(s) 79, 222
Tru1I TTAA 6 cut(s) 18, 351, 665, 720, 729, 1142
Tru9I TTAA 6 cut(s) 18, 351, 665, 720, 729, 1142
TscAI CASTG 2 cut(s) 1063, 1239
TseFI GTSAC 2 cut(s) 244, 593
TseI GCWGC 6 cut(s) 202, 296, 305, 788, 1070, 1157
Tsp45I GTSAC 2 cut(s) 244, 593
TspDTI ATGAA 3 cut(s) 1016, 1274, 1293
TspRI CASTG 2 cut(s) 1063, 1239
VpaK11BI GGWCC 2 cut(s) 1223, 1342
VspI ATTAAT 1 cut(s) 720
XapI RAATTY 2 cut(s) 749, 1097
XmiI GTMKAC 1 cut(s) 1296
XspI CTAG 3 cut(s) 602, 816, 1313
ZrmI AGTACT 2 cut(s) 551, 1105
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.