Rh7AG079200

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0001364
Physical Location & Seq
Forward (+)
5691278 .. 5694489
3212 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG079200.1

Sequence Viewer

Length: 975 bp
ATGAGCATCGAGGTTTTCAACAGGTTTGTGAGGCTTGCGGCCAGAGCGTTCTACGATGACGTCACGCCGGAAAGTGATAATAAGCAGCCCAAGACTGGCCGGAGCGACAATAGGGGAATCGCCGTCGTGACAGCTAAGGGTGTGAAGATTTTCAGTGCTGCGGTTGCTGCCACAGCCAATGGGCAACAACACAGAAAAGAAGGCGAAGAGAAAATTAAGCTGCAGACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCTTTTGAAGAAAGAACTGAAAGATGAACTGGAGGGGAAGCACGCAGTGGAGGAATATGTATGCCATAACTGCAGGAAAAGATACAACGCATTGGATGCACTGCAACTGGTTTCTATGGAGGATGAATACTTCCACTGCGAAAGTTGCAATGGGATACTTGTGGCTGAGAGTGACAAGCTAGATTCTCAAACCACAGCAGGCCGAGACTGCAAAGCAATGCTCCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGAGTTAAAGATTTACCATTTCCTGAATTTGGAAGTCTCCAAGCTTGGGAAGCCAGAGCAAGTGCTCCACGACTTGCAGAGAATAGTGAATCTAGTGCCATTGATCCCTCCATATCTTCTCATGGGCTGGGGTATGGTGGGATGCCCATGCCTTACGTTGGAGATACAAAGGTTGAAGTTGTGTTTTCTAAGAATGAGGGCAAGGGTAAGGATATAAAATCTCAAGCTGATAATACAGACCTAAAAGTTATACCACCTTGGATGATCAAGCAAGAGATGACTCTTACAAGGGAACAGTGTGGAGAGGTCAGGCAAATGAAATCCTCACAAATGCACGTCTCTAGTCATCAGGTTCGTCGGGTTGGCATGAAGTCAGAACGGGATGAGGATGAAGGAGATGATAATATTGACTGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

324

Amino Acids

36.64

Weight (kDa)

5.7

Isoelectric Point (pI)

43.85

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 517
AciI CCGC 2 cut(s) 38, 161
AclWI GGATC 1 cut(s) 653
AcoI YGGCCR 2 cut(s) 39, 97
AcsI RAATTY 1 cut(s) 581
AcuI CTGAAG 1 cut(s) 551
AcyI GRCGYC 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 334
AfaI GTAC 2 cut(s) 278, 525
AfiI CCNNNNNNNGG 5 cut(s) 95, 517, 584, 601, 713
AgsI TTSAA 3 cut(s) 19, 295, 731
AjiI CACGTC 1 cut(s) 892
AluBI AGCT 6 cut(s) 134, 220, 466, 529, 599, 782
AluI AGCT 6 cut(s) 134, 220, 466, 529, 599, 782
Alw21I GWGCWC 1 cut(s) 622
Alw26I GTCTC 3 cut(s) 486, 596, 898
AlwI GGATC 1 cut(s) 653
AoxI GGCC 3 cut(s) 39, 97, 487
ApeKI GCWGC 4 cut(s) 85, 158, 167, 220
ApoI RAATTY 1 cut(s) 581
AseI ATTAAT 1 cut(s) 552
Asp700I GAANNNNTTC 1 cut(s) 149
BaeI ACNNNNGTAYC 2 cut(s) 711, 744
Bbv12I GWGCWC 1 cut(s) 622
BbvI GCAGC 4 cut(s) 97, 145, 154, 207
BceAI ACGGC 1 cut(s) 107
BciVI GTATCC 1 cut(s) 435
BclI TGATCA 1 cut(s) 819
BcoDI GTCTC 3 cut(s) 486, 596, 898
BfaI CTAG 3 cut(s) 467, 648, 897
BfmI CTRYAG 2 cut(s) 221, 358
BfuI GTATCC 1 cut(s) 435
BisI GCNGC 5 cut(s) 39, 86, 159, 168, 221
BlsI GCNGC 5 cut(s) 40, 87, 160, 169, 222
BmgBI CACGTC 1 cut(s) 892
BmsI GCATC 3 cut(s) 15, 373, 687
BpmI CTGGAG 1 cut(s) 338
Bpu10I CCTNAGC 1 cut(s) 135
BpuEI CTTGAG 1 cut(s) 762
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 812
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 517, 584, 601, 713
Bse1I ACTGG 4 cut(s) 100, 321, 399, 971
Bse3DI GCAATG 2 cut(s) 442, 510
BseDI CCNNGG 1 cut(s) 812
BseGI GGATG 6 cut(s) 388, 415, 702, 822, 943, 949
BseLI CCNNNNNNNGG 5 cut(s) 95, 517, 584, 601, 713
BseMI GCAATG 2 cut(s) 442, 510
BseMII CTCAG 2 cut(s) 269, 444
BseNI ACTGG 4 cut(s) 100, 321, 399, 971
BseXI GCAGC 4 cut(s) 97, 145, 154, 207
BseYI CCCAGC 1 cut(s) 682
BsgI GTGCAG 1 cut(s) 269
BshFI GGCC 3 cut(s) 41, 99, 489
BsiHKAI GWGCWC 1 cut(s) 622
BsiSI CCGG 2 cut(s) 68, 100
BslI CCNNNNNNNGG 5 cut(s) 95, 517, 584, 601, 713
BsmAI GTCTC 3 cut(s) 486, 596, 898
BsmBI CGTCTC 1 cut(s) 898
BsnI GGCC 3 cut(s) 41, 99, 489
Bsp1286I GDGCHC 1 cut(s) 622
Bsp143I GATC 2 cut(s) 658, 819
BspACI CCGC 2 cut(s) 38, 161
BspANI GGCC 3 cut(s) 41, 99, 489
BspCNI CTCAG 2 cut(s) 268, 445
BspMAI CTGCAG 2 cut(s) 225, 362
BspPI GGATC 1 cut(s) 653
BsrDI GCAATG 2 cut(s) 442, 510
BsrI ACTGG 4 cut(s) 100, 321, 399, 971
BssECI CCNNGG 1 cut(s) 812
BssMI GATC 2 cut(s) 658, 819
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 812
Bst4CI ACNGT 1 cut(s) 852
Bst6I CTCTTC 1 cut(s) 201
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 3 cut(s) 36, 330, 487
BstDEI CTNAG 4 cut(s) 135, 255, 453, 744
BstF5I GGATG 6 cut(s) 388, 415, 702, 822, 943, 949
BstKTI GATC 2 cut(s) 661, 822
BstMAI GTCTC 3 cut(s) 486, 596, 898
BstMBI GATC 2 cut(s) 658, 819
BstMWI GCNNNNNNNGC 9 cut(s) 44, 164, 167, 173, 357, 383, 432, 495, 605
BstSFI CTRYAG 2 cut(s) 221, 358
BstV1I GCAGC 4 cut(s) 97, 145, 154, 207
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 3 cut(s) 41, 99, 489
BtrI CACGTC 1 cut(s) 892
BtsCI GGATG 6 cut(s) 388, 415, 702, 822, 943, 949
BtsI GCAGTG 3 cut(s) 339, 386, 421
BtsIMutI CAGTG 5 cut(s) 160, 339, 386, 421, 857
Cac8I GCNNGC 3 cut(s) 36, 330, 487
Csp6I GTAC 2 cut(s) 277, 524
CviAII CATG 3 cut(s) 677, 703, 922
CviQI GTAC 2 cut(s) 277, 524
DdeI CTNAG 4 cut(s) 135, 255, 453, 744
DpnI GATC 2 cut(s) 660, 821
DpnII GATC 2 cut(s) 658, 819
DraIII CACNNNGTG 1 cut(s) 334
EaeI YGGCCR 2 cut(s) 39, 97
Eam1104I CTCTTC 1 cut(s) 201
EarI CTCTTC 1 cut(s) 201
Eco130I CCWWGG 1 cut(s) 812
Eco57I CTGAAG 1 cut(s) 551
EcoT14I CCWWGG 1 cut(s) 812
ErhI CCWWGG 1 cut(s) 812
Esp3I CGTCTC 1 cut(s) 898
FaeI CATG 3 cut(s) 680, 706, 925
FatI CATG 3 cut(s) 676, 702, 921
FbaI TGATCA 1 cut(s) 819
Fnu4HI GCNGC 5 cut(s) 39, 86, 159, 168, 221
FokI GGATG 6 cut(s) 395, 422, 709, 829, 950, 956
Fsp4HI GCNGC 5 cut(s) 39, 86, 159, 168, 221
FspBI CTAG 3 cut(s) 467, 648, 897
GluI GCNGC 5 cut(s) 39, 86, 159, 168, 221
GsaI CCCAGC 1 cut(s) 686
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 3 cut(s) 41, 99, 489
HapII CCGG 2 cut(s) 68, 100
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 3 cut(s) 680, 706, 925
HindIII AAGCTT 1 cut(s) 597
HinfI GANTC 5 cut(s) 117, 226, 470, 644, 835
HpaII CCGG 2 cut(s) 68, 100
Hpy188I TCNGA 2 cut(s) 258, 931
Hpy188III TCNNGA 3 cut(s) 127, 245, 578
Hpy99I CGWCG 2 cut(s) 128, 915
HpyAV CCTTC 2 cut(s) 194, 941
HpyCH4III ACNGT 1 cut(s) 852
HpyCH4IV ACGT 3 cut(s) 60, 711, 891
HpyCH4V TGCA 9 cut(s) 223, 286, 360, 386, 391, 435, 498, 632, 889
HpyF10VI GCNNNNNNNGC 9 cut(s) 44, 164, 167, 173, 357, 383, 432, 495, 605
HpyF3I CTNAG 4 cut(s) 135, 255, 453, 744
HpySE526I ACGT 3 cut(s) 60, 711, 891
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 3 cut(s) 680, 706, 925
Ksp22I TGATCA 1 cut(s) 819
Kzo9I GATC 2 cut(s) 658, 819
LmnI GCTCC 3 cut(s) 102, 513, 625
Lsp1109I GCAGC 4 cut(s) 97, 145, 154, 207
LweI GCATC 3 cut(s) 15, 373, 687
MaeI CTAG 3 cut(s) 467, 648, 897
MaeII ACGT 3 cut(s) 60, 711, 891
MaeIII GTNAC 3 cut(s) 61, 127, 458
MalI GATC 2 cut(s) 660, 821
MboI GATC 2 cut(s) 658, 819
MboII GAAGA 4 cut(s) 157, 218, 307, 663
MhlI GDGCHC 1 cut(s) 622
MluCI AATT 3 cut(s) 213, 549, 581
MlyI GAGTC 2 cut(s) 220, 829
MmeI TCCRAC 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 149
MseI TTAA 4 cut(s) 216, 539, 552, 561
MspA1I CMGCKG 1 cut(s) 529
MspI CCGG 2 cut(s) 68, 100
MwoI GCNNNNNNNGC 9 cut(s) 44, 164, 167, 173, 357, 383, 432, 495, 605
NdeII GATC 2 cut(s) 658, 819
NlaIII CATG 3 cut(s) 680, 706, 925
NmeAIII GCCGAG 1 cut(s) 515
NmuCI GTSAC 3 cut(s) 61, 127, 458
PdmI GAANNNNTTC 1 cut(s) 149
PfeI GAWTC 3 cut(s) 117, 470, 644
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 5 cut(s) 40, 87, 160, 169, 222
PleI GAGTC 2 cut(s) 220, 829
PpsI GAGTC 2 cut(s) 220, 829
PshBI ATTAAT 1 cut(s) 552
PspFI CCCAGC 1 cut(s) 682
PstI CTGCAG 2 cut(s) 225, 362
PvuII CAGCTG 1 cut(s) 529
RsaI GTAC 2 cut(s) 278, 525
RsaNI GTAC 2 cut(s) 277, 524
SaqAI TTAA 4 cut(s) 216, 539, 552, 561
SatI GCNGC 5 cut(s) 39, 86, 159, 168, 221
Sau3AI GATC 2 cut(s) 658, 819
SchI GAGTC 2 cut(s) 220, 829
SduI GDGCHC 1 cut(s) 622
SfaNI GCATC 3 cut(s) 15, 373, 687
SfcI CTRYAG 2 cut(s) 221, 358
SmlI CTYRAG 1 cut(s) 777
SmoI CTYRAG 1 cut(s) 777
Sse9I AATT 3 cut(s) 213, 549, 581
SsiI CCGC 2 cut(s) 38, 161
SspI AATATT 1 cut(s) 961
SspMI CTAG 3 cut(s) 467, 648, 897
StyI CCWWGG 1 cut(s) 812
TaaI ACNGT 1 cut(s) 852
TaiI ACGT 3 cut(s) 63, 714, 894
TaqI TCGA 1 cut(s) 9
TasI AATT 3 cut(s) 213, 549, 581
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 117, 470, 644
Tru1I TTAA 4 cut(s) 216, 539, 552, 561
Tru9I TTAA 4 cut(s) 216, 539, 552, 561
TscAI CASTG 5 cut(s) 160, 339, 393, 428, 857
TseFI GTSAC 3 cut(s) 61, 127, 458
TseI GCWGC 4 cut(s) 85, 158, 167, 220
Tsp45I GTSAC 3 cut(s) 61, 127, 458
TspDTI ATGAA 5 cut(s) 327, 426, 887, 938, 960
TspRI CASTG 5 cut(s) 160, 339, 393, 428, 857
Van91I CCANNNNNTGG 1 cut(s) 517
VspI ATTAAT 1 cut(s) 552
XapI RAATTY 1 cut(s) 581
XmnI GAANNNNTTC 1 cut(s) 149
XspI CTAG 3 cut(s) 467, 648, 897
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.