RLG00000004967

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001364
Physical Location & Seq
Forward (+)
64060750 .. 64064176
3427 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004967

Sequence Viewer

Length: 807 bp
ATGAGCATCGAGGTTTTCAACAGGTTTGTGAGGCTTGCGGCCAGAGCGTTCTACGATGACGTCACGCTGGAAAGTGATAATAAGCAGCCCAAGACTGGCCGGAGCGACAATAAGGGAATCGCCGTCGTGGTGCTCGACGCGCTATCGAGAATGATGTGGATTAGGTCTACAGCTAAGGGTGTGAAGATTTTCAGTGCTGCGGTTGCTACCACAGCCAATGGGCAACAACACAGAAAAGAAGGCGAAGAGAAAATTAAGCTGCAGACTAACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCTTTTGAAGAAAGAACTGAAAGAAGAACTGGAGGGGAAGCACGCAGTGGAGGAATATGCATGCCATAACTGCAGGAAAAGATACAACGCATTGGATGCACTGCAACTGGTTTCTATGGAGGATGAGTACTTCCACTGCGAAAGTTGCAATGGGATACTTGTGGCCGAGAGTGTCAAGCTAGATTCTCAAAACACAGCAGGCCGAGACTGCAAAGCAATGCTTCAAAATATGGAGGTTGAAGTTGTGTTTTCTAAGAATGAGGACAAGGGTAAGGATATAAAATCTCAAGCTGATAATACAGACCTAAAAGTTGTACCACCTTGGATGATCAAGCAAGAGATGACTCTTACAAGGGAACAGTGTGGAGAGGTCAGGCAAATGGAATCCTCACAAATGCACGTCTCTAGTAATGCAAATGAAAATTACAAGGTCAATGACTTGAATGTCGAAGCAGATGCTACCGCAGAGGATAAGGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

269

Amino Acids

30.45

Weight (kDa)

5.65

Isoelectric Point (pI)

44.59

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 770
AatII GACGTC 1 cut(s) 63
AccI GTMKAC 1 cut(s) 167
AccII CGCG 1 cut(s) 140
AciI CCGC 3 cut(s) 38, 200, 789
AcoI YGGCCR 3 cut(s) 39, 97, 489
AcyI GRCGYC 1 cut(s) 60
AdeI CACNNNGTG 1 cut(s) 373
AfaI GTAC 3 cut(s) 317, 455, 642
AfiI CCNNNNNNNGG 1 cut(s) 95
AgsI TTSAA 5 cut(s) 19, 334, 551, 566, 769
AjiI CACGTC 1 cut(s) 727
AluBI AGCT 4 cut(s) 173, 259, 505, 617
AluI AGCT 4 cut(s) 173, 259, 505, 617
Alw21I GWGCWC 1 cut(s) 135
Alw26I GTCTC 2 cut(s) 525, 733
AoxI GGCC 4 cut(s) 39, 97, 489, 526
ApeKI GCWGC 3 cut(s) 85, 197, 259
Asp700I GAANNNNTTC 1 cut(s) 188
AspLEI GCGC 1 cut(s) 142
Bbv12I GWGCWC 1 cut(s) 135
BbvI GCAGC 3 cut(s) 97, 184, 246
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 764, 798
BciVI GTATCC 1 cut(s) 474
BclI TGATCA 1 cut(s) 654
BcoDI GTCTC 2 cut(s) 525, 733
BfaI CTAG 2 cut(s) 506, 732
BfmI CTRYAG 3 cut(s) 168, 260, 397
BfuI GTATCC 1 cut(s) 474
BisI GCNGC 4 cut(s) 39, 86, 198, 260
BlsI GCNGC 4 cut(s) 40, 87, 199, 261
BmcAI AGTACT 1 cut(s) 455
BmgBI CACGTC 1 cut(s) 727
BmsI GCATC 3 cut(s) 15, 412, 772
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 597
BsaHI GRCGYC 1 cut(s) 60
BsaJI CCNNGG 1 cut(s) 647
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 1 cut(s) 95
Bse1I ACTGG 3 cut(s) 100, 360, 438
Bse3DI GCAATG 2 cut(s) 481, 549
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 3 cut(s) 427, 454, 657
BseLI CCNNNNNNNGG 1 cut(s) 95
BseMI GCAATG 2 cut(s) 481, 549
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 3 cut(s) 100, 360, 438
BseXI GCAGC 3 cut(s) 97, 184, 246
BsgI GTGCAG 1 cut(s) 308
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 4 cut(s) 41, 99, 491, 528
BsiHKAI GWGCWC 1 cut(s) 135
BsiSI CCGG 1 cut(s) 100
BslI CCNNNNNNNGG 1 cut(s) 95
BsmAI GTCTC 2 cut(s) 525, 733
BsmBI CGTCTC 1 cut(s) 733
BsnI GGCC 4 cut(s) 41, 99, 491, 528
Bsp1286I GDGCHC 1 cut(s) 135
Bsp143I GATC 1 cut(s) 654
BspACI CCGC 3 cut(s) 38, 200, 789
BspANI GGCC 4 cut(s) 41, 99, 491, 528
BspCNI CTCAG 1 cut(s) 307
BspFNI CGCG 1 cut(s) 140
BspMAI CTGCAG 2 cut(s) 264, 401
BsrDI GCAATG 2 cut(s) 481, 549
BsrI ACTGG 3 cut(s) 100, 360, 438
BssECI CCNNGG 1 cut(s) 647
BssMI GATC 1 cut(s) 654
BssNI GRCGYC 1 cut(s) 60
BssT1I CCWWGG 1 cut(s) 647
Bst4CI ACNGT 1 cut(s) 687
Bst6I CTCTTC 1 cut(s) 240
BstACI GRCGYC 1 cut(s) 60
BstAPI GCANNNNNTGC 1 cut(s) 422
BstC8I GCNNGC 4 cut(s) 36, 369, 388, 526
BstDEI CTNAG 3 cut(s) 174, 294, 579
BstF5I GGATG 3 cut(s) 427, 454, 657
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 1 cut(s) 657
BstMAI GTCTC 2 cut(s) 525, 733
BstMBI GATC 1 cut(s) 654
BstMWI GCNNNNNNNGC 8 cut(s) 44, 139, 203, 212, 396, 422, 471, 534
BstNSI RCATGY 1 cut(s) 390
BstSFI CTRYAG 3 cut(s) 168, 260, 397
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 3 cut(s) 97, 184, 246
BsuI GTATCC 1 cut(s) 474
BsuRI GGCC 4 cut(s) 41, 99, 491, 528
BtrI CACGTC 1 cut(s) 727
BtsCI GGATG 3 cut(s) 427, 454, 657
BtsI GCAGTG 3 cut(s) 378, 425, 460
BtsIMutI CAGTG 5 cut(s) 199, 378, 425, 460, 692
Cac8I GCNNGC 4 cut(s) 36, 369, 388, 526
CfoI GCGC 1 cut(s) 142
CseI GACGC 1 cut(s) 146
Csp6I GTAC 3 cut(s) 316, 454, 641
CviAII CATG 1 cut(s) 387
CviQI GTAC 3 cut(s) 316, 454, 641
DdeI CTNAG 3 cut(s) 174, 294, 579
DpnI GATC 1 cut(s) 656
DpnII GATC 1 cut(s) 654
DraIII CACNNNGTG 1 cut(s) 373
DrdI GACNNNNNNGTC 1 cut(s) 770
DseDI GACNNNNNNGTC 1 cut(s) 770
EaeI YGGCCR 3 cut(s) 39, 97, 489
Eam1104I CTCTTC 1 cut(s) 240
EarI CTCTTC 1 cut(s) 240
Eco130I CCWWGG 1 cut(s) 647
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 388
ErhI CCWWGG 1 cut(s) 647
Esp3I CGTCTC 1 cut(s) 733
FaeI CATG 1 cut(s) 390
FaiI YATR 9 cut(s) 291, 301, 303, 384, 388, 393, 443, 557, 605
FalI AAGNNNNNCTT 2 cut(s) 531, 563
FatI CATG 1 cut(s) 386
FbaI TGATCA 1 cut(s) 654
FblI GTMKAC 1 cut(s) 167
Fnu4HI GCNGC 4 cut(s) 39, 86, 198, 260
FokI GGATG 3 cut(s) 434, 461, 664
Fsp4HI GCNGC 4 cut(s) 39, 86, 198, 260
FspBI CTAG 2 cut(s) 506, 732
GlaI GCGC 1 cut(s) 141
GluI GCNGC 4 cut(s) 39, 86, 198, 260
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 4 cut(s) 41, 99, 491, 528
HapII CCGG 1 cut(s) 100
HgaI GACGC 1 cut(s) 146
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 1 cut(s) 60
Hin1II CATG 1 cut(s) 390
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HinfI GANTC 4 cut(s) 117, 509, 670, 710
HpaII CCGG 1 cut(s) 100
Hpy166II GTNNAC 1 cut(s) 168
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 2 cut(s) 147, 284
Hpy8I GTNNAC 1 cut(s) 168
Hpy99I CGWCG 2 cut(s) 128, 140
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 687
HpyCH4IV ACGT 2 cut(s) 60, 726
HpyF10VI GCNNNNNNNGC 8 cut(s) 44, 139, 203, 212, 396, 422, 471, 534
HpyF3I CTNAG 3 cut(s) 174, 294, 579
HpySE526I ACGT 2 cut(s) 60, 726
Hsp92I GRCGYC 1 cut(s) 60
Hsp92II CATG 1 cut(s) 390
HspAI GCGC 1 cut(s) 140
Ksp22I TGATCA 1 cut(s) 654
Kzo9I GATC 1 cut(s) 654
LmnI GCTCC 1 cut(s) 102
Lsp1109I GCAGC 3 cut(s) 97, 184, 246
LweI GCATC 3 cut(s) 15, 412, 772
MaeI CTAG 2 cut(s) 506, 732
MaeII ACGT 2 cut(s) 60, 726
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 656
MboI GATC 1 cut(s) 654
MboII GAAGA 4 cut(s) 196, 257, 346, 362
MhlI GDGCHC 1 cut(s) 135
MluCI AATT 2 cut(s) 252, 748
MlyI GAGTC 1 cut(s) 664
Mph1103I ATGCAT 1 cut(s) 388
MroXI GAANNNNTTC 1 cut(s) 188
MseI TTAA 1 cut(s) 255
MspI CCGG 1 cut(s) 100
MvnI CGCG 1 cut(s) 140
MwoI GCNNNNNNNGC 8 cut(s) 44, 139, 203, 212, 396, 422, 471, 534
NdeII GATC 1 cut(s) 654
NlaIII CATG 1 cut(s) 390
NmeAIII GCCGAG 2 cut(s) 517, 554
NmuCI GTSAC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 388
NspI RCATGY 1 cut(s) 390
PaeI GCATGC 1 cut(s) 390
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 188
PfeI GAWTC 3 cut(s) 117, 509, 710
PkrI GCNGC 4 cut(s) 40, 87, 199, 261
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PstI CTGCAG 2 cut(s) 264, 401
RsaI GTAC 3 cut(s) 317, 455, 642
RsaNI GTAC 3 cut(s) 316, 454, 641
SaqAI TTAA 1 cut(s) 255
SatI GCNGC 4 cut(s) 39, 86, 198, 260
Sau3AI GATC 1 cut(s) 654
ScaI AGTACT 1 cut(s) 455
SchI GAGTC 1 cut(s) 664
SduI GDGCHC 1 cut(s) 135
SfaNI GCATC 3 cut(s) 15, 412, 772
SfcI CTRYAG 3 cut(s) 168, 260, 397
SmlI CTYRAG 1 cut(s) 612
SmoI CTYRAG 1 cut(s) 612
SphI GCATGC 1 cut(s) 390
Sse9I AATT 2 cut(s) 252, 748
SsiI CCGC 3 cut(s) 38, 200, 789
SspMI CTAG 2 cut(s) 506, 732
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 1 cut(s) 687
TaiI ACGT 2 cut(s) 63, 729
TaqI TCGA 4 cut(s) 9, 135, 146, 774
TasI AATT 2 cut(s) 252, 748
TatI WGTACW 1 cut(s) 453
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 3 cut(s) 117, 509, 710
Tru1I TTAA 1 cut(s) 255
Tru9I TTAA 1 cut(s) 255
TscAI CASTG 5 cut(s) 199, 378, 432, 467, 692
TseFI GTSAC 1 cut(s) 61
TseI GCWGC 3 cut(s) 85, 197, 259
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 759
TspRI CASTG 5 cut(s) 199, 378, 432, 467, 692
XceI RCATGY 1 cut(s) 390
XmiI GTMKAC 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 188
XspI CTAG 2 cut(s) 506, 732
ZraI GACGTC 1 cut(s) 61
ZrmI AGTACT 1 cut(s) 455
Zsp2I ATGCAT 1 cut(s) 388
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.