FvH4_7g21490

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0001364
Physical Location & Seq
Forward (+)
17183617 .. 17184770
1154 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g21490.t1

Sequence Viewer

Length: 1062 bp
ATGACGAGTATCGAGCCTTTCAACAGGTTGGTGAGGCTTGTGGCCGGAGCGTTCTACGATGATGATATTTCATCGAAAACGGATAATTCTCAGCCCAAGAGTGGCCGGAGCGTCGACAATAGAGGAATCGGGGTGGTTGTGCTTGAAGCGCTCTCGAGAAGACAATGGGTAAGAGAAGAAGATTTGGCCAAGGACTTGAATCTCAACTCGAAGCAACTTCGTCGTACTTTGCGATTTTTCGAGGAGGAAAAGCTGGTCACCCGAGACCATAGGAAAGAACGACATGCAAAGAAAGAAGGGGAAGAGAAAATCAAGATGTATACGTACTCTTACTGCTGTCTAGACTATGCTCAGATGTATGATGTAGTGAGGTACAGACTGCATAGAATGAAGAAAAAGTTGAAAGAGAAATTGGAGGACAAGAACACAGTACAACAATACTTGTGCAAGAATTGTGGGAGAAGATATGACGCGTTGGATGCACTGAGATTGATTTCGATGGAGGATGGTGACTTTCACTGTGAAGAATGCAAAGGGAAACTTGTGGCGGTTGAGACGGACAATGATGTAGCTTCTCCACAAATAGGAGATGGAGAAAGCAGTATTATTTCAAGGAGACAAATGCTTGGAACACTCCGAGCTTGGGAAGCGCGAGCTAAATCTTCTAGTGGGCTAGGGCAGGATGTTGGAGCTACAGAGGTTGAGGTTATTACTTGGGTTAAAGAAGACAAGGGAGACGATACTAAATCGGAATTAGATGATACTACTACAAGGGTGGAGAAAGTATATCCACCCTGGTTGAGCAAGCCAGGGATGAACCTTCCAAATTACGAACAACAGGGAGAGTTTTCAGATGACATAAAGGAAAAAGCTCATGAAGATAATGAGAGCAAGAGTAGTACTTTCCAGGTGGAGTGGTTAAGAACTTATTATGCTGCTCTTTTACAAAAACATGAAGAAGAAGAAGAAAGAAATACTCGCGAGGTTGGCATGAAGTCAAAACGTGATCAGAAAGAAGAAGAAGAAGATGAAGATGAAGAAGATATCTGGGAGGAAGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

354

Amino Acids

41.17

Weight (kDa)

5.1

Isoelectric Point (pI)

54.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 45 - 141 4.2e-12 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 114, 320
AccII CGCG 3 cut(s) 473, 652, 981
AciI CCGC 1 cut(s) 548
AcoI YGGCCR 3 cut(s) 42, 103, 186
AfaI GTAC 5 cut(s) 226, 326, 374, 432, 901
AfeI AGCGCT 1 cut(s) 150
AfiI CCNNNNNNNGG 3 cut(s) 101, 584, 643
AflIII ACRYGT 1 cut(s) 471
AgsI TTSAA 5 cut(s) 22, 146, 199, 403, 612
AjnI CCWGG 3 cut(s) 794, 808, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 395, 427
AluBI AGCT 6 cut(s) 253, 572, 641, 656, 692, 872
AluI AGCT 6 cut(s) 253, 572, 641, 656, 692, 872
Alw26I GTCTC 4 cut(s) 258, 548, 610, 729
Ama87I CYCGRG 2 cut(s) 154, 261
Aor51HI AGCGCT 1 cut(s) 150
AoxI GGCC 3 cut(s) 42, 103, 186
ApeKI GCWGC 1 cut(s) 935
AspLEI GCGC 2 cut(s) 151, 652
AsuHPI GGTGA 3 cut(s) 43, 250, 521
AvaI CYCGRG 2 cut(s) 154, 261
BalI TGGCCA 1 cut(s) 188
BbsI GAAGAC 2 cut(s) 166, 732
BbvI GCAGC 1 cut(s) 922
BccI CCATC 3 cut(s) 493, 500, 584
BcgI CGANNNNNNTGC 2 cut(s) 203, 237
BciT130I CCWGG 3 cut(s) 796, 810, 908
BclI TGATCA 1 cut(s) 1006
BcoDI GTCTC 4 cut(s) 258, 548, 610, 729
BfaI CTAG 3 cut(s) 341, 666, 674
BfmI CTRYAG 1 cut(s) 693
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 1 cut(s) 936
BlsI GCNGC 1 cut(s) 937
BmcAI AGTACT 1 cut(s) 901
Bme1390I CCNGG 3 cut(s) 796, 810, 908
BmeT110I CYCGRG 2 cut(s) 154, 261
BmrFI CCNGG 3 cut(s) 796, 810, 908
BmsI GCATC 1 cut(s) 469
BpiI GAAGAC 2 cut(s) 166, 732
BsaAI YACGTR 1 cut(s) 324
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 189, 794, 809
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 584, 643
BseBI CCWGG 3 cut(s) 796, 810, 908
BseDI CCNNGG 3 cut(s) 189, 794, 809
BseGI GGATG 4 cut(s) 484, 511, 688, 819
BseLI CCNNNNNNNGG 3 cut(s) 101, 584, 643
BseMII CTCAG 3 cut(s) 104, 365, 476
BseRI GAGGAG 1 cut(s) 257
BseXI GCAGC 1 cut(s) 922
Bsh1236I CGCG 3 cut(s) 473, 652, 981
BshFI GGCC 3 cut(s) 44, 105, 188
BsiHKCI CYCGRG 2 cut(s) 154, 261
BsiSI CCGG 2 cut(s) 45, 106
BslI CCNNNNNNNGG 3 cut(s) 101, 584, 643
BsmAI GTCTC 4 cut(s) 258, 548, 610, 729
BsmBI CGTCTC 2 cut(s) 548, 729
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 3 cut(s) 44, 105, 188
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 2 cut(s) 154, 261
Bsp143I GATC 1 cut(s) 1006
Bsp68I TCGCGA 1 cut(s) 981
BspACI CCGC 1 cut(s) 548
BspANI GGCC 3 cut(s) 44, 105, 188
BspCNI CTCAG 3 cut(s) 103, 364, 477
BspFNI CGCG 3 cut(s) 473, 652, 981
BspHI TCATGA 1 cut(s) 874
BspTNI GGTCTC 1 cut(s) 258
BssECI CCNNGG 3 cut(s) 189, 794, 809
BssMI GATC 1 cut(s) 1006
BssNAI GTATAC 1 cut(s) 321
BssT1I CCWWGG 1 cut(s) 189
Bst1107I GTATAC 1 cut(s) 321
Bst2UI CCWGG 3 cut(s) 796, 810, 908
Bst4CI ACNGT 2 cut(s) 430, 521
Bst6I CTCTTC 1 cut(s) 297
BstBAI YACGTR 1 cut(s) 324
BstC8I GCNNGC 2 cut(s) 654, 806
BstDEI CTNAG 3 cut(s) 90, 351, 485
BstEII GGTNACC 1 cut(s) 256
BstF5I GGATG 4 cut(s) 484, 511, 688, 819
BstFNI CGCG 3 cut(s) 473, 652, 981
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 2 cut(s) 151, 652
BstKTI GATC 1 cut(s) 1009
BstMAI GTCTC 4 cut(s) 258, 548, 610, 729
BstMBI GATC 1 cut(s) 1006
BstMWI GCNNNNNNNGC 4 cut(s) 148, 479, 647, 987
BstNI CCWGG 3 cut(s) 796, 810, 908
BstNSI RCATGY 1 cut(s) 287
BstPI GGTNACC 1 cut(s) 256
BstSCI CCNGG 3 cut(s) 794, 808, 906
BstSFI CTRYAG 1 cut(s) 693
BstSNI TACGTA 1 cut(s) 324
BstUI CGCG 3 cut(s) 473, 652, 981
BstV1I GCAGC 1 cut(s) 922
BstV2I GAAGAC 2 cut(s) 166, 732
BstZ17I GTATAC 1 cut(s) 321
BsuRI GGCC 3 cut(s) 44, 105, 188
BtsCI GGATG 4 cut(s) 484, 511, 688, 819
BtsIMutI CAGTG 2 cut(s) 482, 517
BtuMI TCGCGA 1 cut(s) 981
Cac8I GCNNGC 2 cut(s) 654, 806
CciI TCATGA 1 cut(s) 874
CfoI GCGC 2 cut(s) 151, 652
CseI GACGC 2 cut(s) 100, 479
Csp6I GTAC 5 cut(s) 225, 325, 373, 431, 900
CspCI CAANNNNNGTGG 4 cut(s) 436, 471, 780, 815
CviAII CATG 4 cut(s) 284, 875, 953, 991
CviQI GTAC 5 cut(s) 225, 325, 373, 431, 900
DdeI CTNAG 3 cut(s) 90, 351, 485
DpnI GATC 1 cut(s) 1008
DpnII GATC 1 cut(s) 1006
EaeI YGGCCR 3 cut(s) 42, 103, 186
Eam1104I CTCTTC 1 cut(s) 297
EarI CTCTTC 1 cut(s) 297
Eco105I TACGTA 1 cut(s) 324
Eco130I CCWWGG 1 cut(s) 189
Eco31I GGTCTC 1 cut(s) 258
Eco32I GATATC 1 cut(s) 1045
Eco47III AGCGCT 1 cut(s) 150
Eco88I CYCGRG 2 cut(s) 154, 261
Eco91I GGTNACC 1 cut(s) 256
EcoO65I GGTNACC 1 cut(s) 256
EcoRII CCWGG 3 cut(s) 794, 808, 906
EcoRV GATATC 1 cut(s) 1045
EcoT14I CCWWGG 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 2 cut(s) 548, 729
FaeI CATG 4 cut(s) 287, 878, 956, 994
FalI AAGNNNNNCTT 2 cut(s) 525, 557
FatI CATG 4 cut(s) 283, 874, 952, 990
FbaI TGATCA 1 cut(s) 1006
FblI GTMKAC 2 cut(s) 114, 320
Fnu4HI GCNGC 1 cut(s) 936
FokI GGATG 4 cut(s) 491, 518, 695, 826
Fsp4HI GCNGC 1 cut(s) 936
FspBI CTAG 3 cut(s) 341, 666, 674
GlaI GCGC 2 cut(s) 150, 651
GluI GCNGC 1 cut(s) 936
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 3 cut(s) 44, 105, 188
HapII CCGG 2 cut(s) 45, 106
HgaI GACGC 2 cut(s) 100, 479
HhaI GCGC 2 cut(s) 151, 652
Hin1II CATG 4 cut(s) 287, 878, 956, 994
Hin6I GCGC 2 cut(s) 149, 650
HinP1I GCGC 2 cut(s) 149, 650
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 2 cut(s) 126, 199
HpaII CCGG 2 cut(s) 45, 106
HphI GGTGA 3 cut(s) 43, 250, 521
Hpy166II GTNNAC 2 cut(s) 115, 321
Hpy188I TCNGA 5 cut(s) 354, 638, 751, 853, 1011
Hpy188III TCNNGA 6 cut(s) 154, 156, 313, 341, 875, 980
Hpy8I GTNNAC 2 cut(s) 115, 321
Hpy99I CGWCG 2 cut(s) 116, 225
HpyAV CCTTC 3 cut(s) 290, 830, 1049
HpyCH4III ACNGT 2 cut(s) 430, 521
HpyCH4IV ACGT 2 cut(s) 323, 1003
HpyCH4V TGCA 5 cut(s) 287, 382, 447, 482, 531
HpyF10VI GCNNNNNNNGC 4 cut(s) 148, 479, 647, 987
HpyF3I CTNAG 3 cut(s) 90, 351, 485
HpySE526I ACGT 2 cut(s) 323, 1003
Hsp92II CATG 4 cut(s) 287, 878, 956, 994
HspAI GCGC 2 cut(s) 149, 650
Ksp22I TGATCA 1 cut(s) 1006
Kzo9I GATC 1 cut(s) 1006
LmnI GCTCC 3 cut(s) 47, 108, 689
Lsp1109I GCAGC 1 cut(s) 922
LweI GCATC 1 cut(s) 469
MaeI CTAG 3 cut(s) 341, 666, 674
MaeII ACGT 2 cut(s) 323, 1003
MaeIII GTNAC 2 cut(s) 256, 509
MalI GATC 1 cut(s) 1008
MboI GATC 1 cut(s) 1006
MlsI TGGCCA 1 cut(s) 188
MluCI AATT 5 cut(s) 85, 410, 451, 752, 826
MluI ACGCGT 1 cut(s) 471
MluNI TGGCCA 1 cut(s) 188
MmeI TCCRAC 2 cut(s) 456, 667
Mox20I TGGCCA 1 cut(s) 188
MscI TGGCCA 1 cut(s) 188
MseI TTAA 2 cut(s) 720, 920
Msp20I TGGCCA 1 cut(s) 188
MspI CCGG 2 cut(s) 45, 106
MspR9I CCNGG 3 cut(s) 796, 810, 908
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 3 cut(s) 796, 810, 908
MvnI CGCG 3 cut(s) 473, 652, 981
MwoI GCNNNNNNNGC 4 cut(s) 148, 479, 647, 987
NdeII GATC 1 cut(s) 1006
NlaIII CATG 4 cut(s) 287, 878, 956, 994
NmuCI GTSAC 2 cut(s) 256, 509
NruI TCGCGA 1 cut(s) 981
NspI RCATGY 1 cut(s) 287
PaeR7I CTCGAG 1 cut(s) 154
PagI TCATGA 1 cut(s) 874
PcsI WCGNNNNNNNCGW 1 cut(s) 229
PctI GAATGC 1 cut(s) 533
PfeI GAWTC 2 cut(s) 126, 199
PkrI GCNGC 1 cut(s) 937
Ppu21I YACGTR 1 cut(s) 324
Psp6I CCWGG 3 cut(s) 794, 808, 906
PspEI GGTNACC 1 cut(s) 256
PspGI CCWGG 3 cut(s) 794, 808, 906
RruI TCGCGA 1 cut(s) 981
RsaI GTAC 5 cut(s) 226, 326, 374, 432, 901
RsaNI GTAC 5 cut(s) 225, 325, 373, 431, 900
SalI GTCGAC 1 cut(s) 113
SaqAI TTAA 2 cut(s) 720, 920
SatI GCNGC 1 cut(s) 936
Sau3AI GATC 1 cut(s) 1006
ScaI AGTACT 1 cut(s) 901
ScrFI CCNGG 3 cut(s) 796, 810, 908
SfaNI GCATC 1 cut(s) 469
SfcI CTRYAG 1 cut(s) 693
Sfr274I CTCGAG 1 cut(s) 154
SlaI CTCGAG 1 cut(s) 154
SmlI CTYRAG 1 cut(s) 154
SmoI CTYRAG 1 cut(s) 154
SnaBI TACGTA 1 cut(s) 324
Sse9I AATT 5 cut(s) 85, 410, 451, 752, 826
SsiI CCGC 1 cut(s) 548
SspMI CTAG 3 cut(s) 341, 666, 674
StyD4I CCNGG 3 cut(s) 794, 808, 906
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 2 cut(s) 430, 521
TaiI ACGT 2 cut(s) 326, 1006
TaqI TCGA 7 cut(s) 12, 74, 114, 155, 209, 240, 497
TasI AATT 5 cut(s) 85, 410, 451, 752, 826
TatI WGTACW 2 cut(s) 430, 899
TfiI GAWTC 2 cut(s) 126, 199
Tru1I TTAA 2 cut(s) 720, 920
Tru9I TTAA 2 cut(s) 720, 920
TscAI CASTG 2 cut(s) 489, 524
TseFI GTSAC 2 cut(s) 256, 509
TseI GCWGC 1 cut(s) 935
Tsp45I GTSAC 2 cut(s) 256, 509
TspDTI ATGAA 8 cut(s) 60, 404, 830, 891, 969, 1007, 1044, 1050
TspGWI ACGGA 2 cut(s) 95, 572
TspRI CASTG 2 cut(s) 489, 524
XbaI TCTAGA 1 cut(s) 340
XceI RCATGY 1 cut(s) 287
XhoI CTCGAG 1 cut(s) 154
XmiI GTMKAC 2 cut(s) 114, 320
XspI CTAG 3 cut(s) 341, 666, 674
ZrmI AGTACT 1 cut(s) 901
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.