pycom06g18820

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr6
OG0001364
Physical Location & Seq
Reverse (-)
22399236 .. 22404042
4807 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom06g18820.7

Sequence Viewer

Length: 1413 bp
ATGAGCATCGAGCCGTTCAACAAGCTGGTGAAGCTTACTGCGAGGGCGTTTTACGATGACATACCGACGAAAGGGGAGAATCAGCCCAAGACCGGACGGAGCGATAATCGGGGGATTGCGGTGGTGGTGCTGGATGCTCTATCGAGACGACAATGGGTAAGGGAAGAAGATTTGGCAAAGGACTTGAAACTGCACTCGAAGCAACTTCGTCGTACTTTGCGATTTTTTGAGGAGGAAAAACTGGTCACCAGAGATCATAGGAGAGAGACAGCTAAGGGTGCAAAGGTGCACAATGCAGCTGTTGCTGCCACAGGAGATGGTCCACCTGTAAAAGAAGGGGAAGAGAAAGTTAAGATGCACACTCACTCGTACTGCTGTCTGGATTATGCACAGATATATGATGTGGTAAGGTACAGACTGCACCGTTTGCGGAAAAAACTGAAAGATGAGTTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCCAACTGTGGGAAAAGATACAACGCTTTAGATGCGCTGCAACTGGTGTCTATGGAGGATGAGTACTTCCATTGTGAAAGTTGCAATGGAGAACTTGTGGCTGAGAGTGACAAGCTAGCTTCGCAAGAAGTAGTAGGAGATGGAGACGACAATGCAAGGAGGCGGCGACGTGAAAAATTAAAAGACTTGCTTCAAAAGATGGAGGTACAGCTGAAGCCTCTAATGGACCAAATAAATAGAGTTAAAGACTTATCTCCTCCTGAATTTGGAAGTCTCCAAGCTTGGGAAGCGCGAGCAAGTGCTGCTAGACGTGCGGAGAATGGTGAGTCTAGTGCCATGGATCCCTCCAAATCTTCTCATGGGATGGGATACGGTGGAACGCCAATGCCCTTTCTTGGAGATACAAAGGTTGAAGTTGATTTCTCTGGGGTTGAAGGTAAAGGAAAGGATATTAAATCTGAAGCTGATGGTACAAGCCTGAAAGTTTTGCCACCTTGGATGATCAAGCAAGGCATGAACCTTACAAAAGAACAACGTGGAGAGGTCAAGCAGGAGCAAAAGATGGATGCCATTGGAAGTGTAGCAGCTCCAGAGTTCTCAGAAGACAAAAAATTCAGTACCGAGAATAATGAGAAAAATATACAGGATGAGTATTTAAAAGCATATTATGCTGCTCTAATAAAAAGACAGCAAGAAGAAGAAGCTAAGAAAGCACAGGAAACACAGATCTCTGATGGTCCCTCCGCAATGTCTCCTAATCGTCGGGTTGGCATGAAGTCAAAGCGCGAGGATGAAGGAGAAGGTAATGTAGACTGGGAAGAGGCTCCAGTAACAGGTAATGCAGAAAATTATAAGGTCCATGACTTGAACGTCGAAGCTGATGCTATAGCGGACGATGAGGATGATATAGACTGGGAGGAAGGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

471

Amino Acids

53.15

Weight (kDa)

5.34

Isoelectric Point (pI)

53.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1338
AasI GACNNNNNNGTC 1 cut(s) 1355
AccI GTMKAC 1 cut(s) 1296
AccII CGCG 2 cut(s) 778, 1272
AciI CCGC 6 cut(s) 119, 430, 649, 800, 1230, 1376
AclWI GGATC 2 cut(s) 821, 834
AcsI RAATTY 2 cut(s) 749, 1097
AcuI CTGAAG 2 cut(s) 719, 966
AfaI GTAC 7 cut(s) 214, 371, 413, 551, 693, 958, 1105
AfiI CCNNNNNNNGG 8 cut(s) 71, 92, 494, 495, 752, 769, 881, 1319
AgsI TTSAA 6 cut(s) 19, 187, 680, 899, 920, 1354
AjiI CACGTC 2 cut(s) 656, 797
AjuI GAANNNNNNNTTGG 2 cut(s) 434, 466
Alw21I GWGCWC 1 cut(s) 291
Alw26I GTCTC 5 cut(s) 139, 260, 624, 764, 1242
Alw44I GTGCAC 1 cut(s) 287
AlwI GGATC 2 cut(s) 821, 834
ApaLI GTGCAC 1 cut(s) 287
ApeKI GCWGC 6 cut(s) 296, 305, 523, 788, 1070, 1157
ApoI RAATTY 2 cut(s) 749, 1097
AspLEI GCGC 3 cut(s) 523, 778, 1272
AspS9I GGNCC 4 cut(s) 320, 712, 1223, 1342
AsuHPI GGTGA 3 cut(s) 40, 238, 821
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 4 cut(s) 320, 712, 1223, 1342
BaeGI GKGCMC 1 cut(s) 291
BaeI ACNNNNGTAYC 2 cut(s) 879, 912
BamHI GGATCC 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 1 cut(s) 291
BbvI GCAGC 6 cut(s) 292, 308, 510, 775, 1082, 1144
BccI CCATC 7 cut(s) 311, 620, 679, 844, 947, 1042, 1214
BcgI CGANNNNNNTGC 4 cut(s) 191, 225, 1349, 1383
BciVI GTATCC 1 cut(s) 848
BclI TGATCA 1 cut(s) 987
BcoDI GTCTC 5 cut(s) 139, 260, 624, 764, 1242
BfaI CTAG 4 cut(s) 602, 792, 816, 1411
BfmI CTRYAG 1 cut(s) 1371
BfuI GTATCC 1 cut(s) 848
BglII AGATCT 1 cut(s) 1212
BisI GCNGC 7 cut(s) 297, 306, 524, 650, 789, 1071, 1158
BlsI GCNGC 7 cut(s) 298, 307, 525, 651, 790, 1072, 1159
BmcAI AGTACT 1 cut(s) 551
Bme18I GGWCC 4 cut(s) 320, 712, 1223, 1342
BmgBI CACGTC 2 cut(s) 656, 797
BmgT120I GGNCC 4 cut(s) 320, 712, 1223, 1342
BmiI GGNNCC 3 cut(s) 828, 1225, 1311
BmrI ACTGGG 2 cut(s) 1309, 1408
BmsI GCATC 6 cut(s) 15, 124, 345, 508, 1042, 1357
BmtI GCTAGC 1 cut(s) 605
BmuI ACTGGG 2 cut(s) 1309, 1408
BpiI GAAGAC 1 cut(s) 1095
BpmI CTGGAG 2 cut(s) 1059, 1296
Bpu10I CCTNAGC 1 cut(s) 273
BsaJI CCNNGG 2 cut(s) 822, 980
BsaWI WCCGGW 1 cut(s) 92
BsaXI ACNNNNNCTCC 4 cut(s) 533, 563, 567, 597
Bsc4I CCNNNNNNNGG 8 cut(s) 71, 92, 494, 495, 752, 769, 881, 1319
Bse1I ACTGG 5 cut(s) 246, 534, 1304, 1313, 1403
Bse3DI GCAATG 2 cut(s) 577, 1239
BseDI CCNNGG 2 cut(s) 822, 980
BseGI GGATG 8 cut(s) 139, 550, 855, 990, 1057, 1138, 1282, 1393
BseLI CCNNNNNNNGG 8 cut(s) 71, 92, 494, 495, 752, 769, 881, 1319
BseMI GCAATG 2 cut(s) 577, 1239
BseMII CTCAG 2 cut(s) 579, 1098
BseNI ACTGG 5 cut(s) 246, 534, 1304, 1313, 1403
BseRI GAGGAG 2 cut(s) 245, 732
BseSI GKGCMC 1 cut(s) 291
BseXI GCAGC 6 cut(s) 292, 308, 510, 775, 1082, 1144
BsgI GTGCAG 2 cut(s) 176, 404
Bsh1236I CGCG 2 cut(s) 778, 1272
BsiHKAI GWGCWC 1 cut(s) 291
BsiSI CCGG 1 cut(s) 93
BslFI GGGAC 1 cut(s) 1209
BslI CCNNNNNNNGG 8 cut(s) 71, 92, 494, 495, 752, 769, 881, 1319
BsmAI GTCTC 5 cut(s) 139, 260, 624, 764, 1242
BsmBI CGTCTC 2 cut(s) 139, 624
BsmFI GGGAC 1 cut(s) 1209
Bsp1286I GDGCHC 1 cut(s) 291
Bsp143I GATC 4 cut(s) 253, 826, 987, 1212
Bsp19I CCATGG 1 cut(s) 822
BspACI CCGC 6 cut(s) 119, 430, 649, 800, 1230, 1376
BspCNI CTCAG 2 cut(s) 580, 1097
BspFNI CGCG 2 cut(s) 778, 1272
BspLI GGNNCC 3 cut(s) 828, 1225, 1311
BspOI GCTAGC 1 cut(s) 605
BspPI GGATC 2 cut(s) 821, 834
BsrDI GCAATG 2 cut(s) 577, 1239
BsrI ACTGG 5 cut(s) 246, 534, 1304, 1313, 1403
BssECI CCNNGG 2 cut(s) 822, 980
BssMI GATC 4 cut(s) 253, 826, 987, 1212
BssT1I CCWWGG 2 cut(s) 822, 980
Bst4CI ACNGT 4 cut(s) 425, 469, 494, 860
Bst6I CTCTTC 2 cut(s) 336, 1299
BstAPI GCANNNNNTGC 4 cut(s) 302, 427, 788, 1154
BstC8I GCNNGC 2 cut(s) 603, 780
BstDEI CTNAG 4 cut(s) 273, 588, 1084, 1191
BstDSI CCRYGG 1 cut(s) 822
BstEII GGTNACC 1 cut(s) 244
BstF5I GGATG 8 cut(s) 139, 550, 855, 990, 1057, 1138, 1282, 1393
BstFNI CGCG 2 cut(s) 778, 1272
BstHHI GCGC 3 cut(s) 523, 778, 1272
BstKTI GATC 4 cut(s) 256, 829, 990, 1215
BstMAI GTCTC 5 cut(s) 139, 260, 624, 764, 1242
BstMBI GATC 4 cut(s) 253, 826, 987, 1212
BstPI GGTNACC 1 cut(s) 244
BstSFI CTRYAG 1 cut(s) 1371
BstSLI GKGCMC 1 cut(s) 291
BstUI CGCG 2 cut(s) 778, 1272
BstV1I GCAGC 6 cut(s) 292, 308, 510, 775, 1082, 1144
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 2 cut(s) 826, 1212
BstYI RGATCY 2 cut(s) 826, 1212
BsuI GTATCC 1 cut(s) 848
BtgI CCRYGG 1 cut(s) 822
BtrI CACGTC 2 cut(s) 656, 797
BtsCI GGATG 8 cut(s) 139, 550, 855, 990, 1057, 1138, 1282, 1393
Cac8I GCNNGC 2 cut(s) 603, 780
CfoI GCGC 3 cut(s) 523, 778, 1272
Cfr13I GGNCC 4 cut(s) 320, 712, 1223, 1342
Csp6I GTAC 7 cut(s) 213, 370, 412, 550, 692, 957, 1104
CviAII CATG 5 cut(s) 823, 845, 1000, 1258, 1346
CviQI GTAC 7 cut(s) 213, 370, 412, 550, 692, 957, 1104
DdeI CTNAG 4 cut(s) 273, 588, 1084, 1191
DpnI GATC 4 cut(s) 255, 828, 989, 1214
DpnII GATC 4 cut(s) 253, 826, 987, 1212
DraI TTTAAA 1 cut(s) 1143
DrdI GACNNNNNNGTC 1 cut(s) 1355
DseDI GACNNNNNNGTC 1 cut(s) 1355
Eam1104I CTCTTC 2 cut(s) 336, 1299
EarI CTCTTC 2 cut(s) 336, 1299
Eco130I CCWWGG 2 cut(s) 822, 980
Eco47I GGWCC 4 cut(s) 320, 712, 1223, 1342
Eco57I CTGAAG 2 cut(s) 719, 966
Eco91I GGTNACC 1 cut(s) 244
EcoO65I GGTNACC 1 cut(s) 244
EcoT14I CCWWGG 2 cut(s) 822, 980
ErhI CCWWGG 2 cut(s) 822, 980
Esp3I CGTCTC 2 cut(s) 139, 624
FaeI CATG 5 cut(s) 826, 848, 1003, 1261, 1349
FalI AAGNNNNNCTT 2 cut(s) 660, 692
FaqI GGGAC 1 cut(s) 1209
FatI CATG 5 cut(s) 822, 844, 999, 1257, 1345
FbaI TGATCA 1 cut(s) 987
FblI GTMKAC 1 cut(s) 1296
Fnu4HI GCNGC 7 cut(s) 297, 306, 524, 650, 789, 1071, 1158
FokI GGATG 8 cut(s) 146, 557, 862, 997, 1064, 1145, 1289, 1400
Fsp4HI GCNGC 7 cut(s) 297, 306, 524, 650, 789, 1071, 1158
FspBI CTAG 4 cut(s) 602, 792, 816, 1411
GlaI GCGC 3 cut(s) 522, 777, 1271
GluI GCNGC 7 cut(s) 297, 306, 524, 650, 789, 1071, 1158
GsuI CTGGAG 2 cut(s) 1059, 1296
HapII CCGG 1 cut(s) 93
HhaI GCGC 3 cut(s) 523, 778, 1272
Hin1II CATG 5 cut(s) 826, 848, 1003, 1261, 1349
Hin6I GCGC 3 cut(s) 521, 776, 1270
HinP1I GCGC 3 cut(s) 521, 776, 1270
HindIII AAGCTT 2 cut(s) 32, 765
HinfI GANTC 2 cut(s) 79, 812
HpaII CCGG 1 cut(s) 93
HphI GGTGA 3 cut(s) 40, 238, 821
Hpy166II GTNNAC 3 cut(s) 289, 323, 1297
Hpy188I TCNGA 3 cut(s) 946, 1087, 1219
Hpy188III TCNNGA 5 cut(s) 144, 380, 473, 746, 1076
Hpy8I GTNNAC 3 cut(s) 289, 323, 1297
Hpy99I CGWCG 5 cut(s) 70, 213, 657, 1251, 1361
HpyAV CCTTC 5 cut(s) 329, 914, 1274, 1280, 1400
HpyCH4III ACNGT 4 cut(s) 425, 469, 494, 860
HpyCH4IV ACGT 4 cut(s) 655, 796, 1021, 1356
HpyF3I CTNAG 4 cut(s) 273, 588, 1084, 1191
HpySE526I ACGT 4 cut(s) 655, 796, 1021, 1356
Hsp92II CATG 5 cut(s) 826, 848, 1003, 1261, 1349
HspAI GCGC 3 cut(s) 521, 776, 1270
Ksp22I TGATCA 1 cut(s) 987
Kzo9I GATC 4 cut(s) 253, 826, 987, 1212
LmnI GCTCC 4 cut(s) 99, 1039, 1078, 1315
Lsp1109I GCAGC 6 cut(s) 292, 308, 510, 775, 1082, 1144
LweI GCATC 6 cut(s) 15, 124, 345, 508, 1042, 1357
MaeI CTAG 4 cut(s) 602, 792, 816, 1411
MaeII ACGT 4 cut(s) 655, 796, 1021, 1356
MaeIII GTNAC 3 cut(s) 244, 593, 1315
MalI GATC 4 cut(s) 255, 828, 989, 1214
MboI GATC 4 cut(s) 253, 826, 987, 1212
MboII GAAGA 8 cut(s) 176, 179, 353, 831, 1100, 1193, 1196, 1316
MflI RGATCY 2 cut(s) 826, 1212
MhlI GDGCHC 1 cut(s) 291
MluCI AATT 4 cut(s) 662, 749, 1097, 1333
MlyI GAGTC 1 cut(s) 821
MmeI TCCRAC 1 cut(s) 432
MseI TTAA 5 cut(s) 351, 665, 729, 939, 1142
MspA1I CMGCKG 2 cut(s) 299, 697
MspI CCGG 1 cut(s) 93
MvnI CGCG 2 cut(s) 778, 1272
NcoI CCATGG 1 cut(s) 822
NdeII GATC 4 cut(s) 253, 826, 987, 1212
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 5 cut(s) 826, 848, 1003, 1261, 1349
NlaIV GGNNCC 3 cut(s) 828, 1225, 1311
NmuCI GTSAC 2 cut(s) 244, 593
PcsI WCGNNNNNNNCGW 1 cut(s) 217
PfeI GAWTC 1 cut(s) 79
PkrI GCNGC 7 cut(s) 298, 307, 525, 651, 790, 1072, 1159
PleI GAGTC 1 cut(s) 820
PpsI GAGTC 1 cut(s) 820
PsiI TTATAA 1 cut(s) 1338
PspEI GGTNACC 1 cut(s) 244
PspN4I GGNNCC 3 cut(s) 828, 1225, 1311
PspPI GGNCC 4 cut(s) 320, 712, 1223, 1342
PsuI RGATCY 2 cut(s) 826, 1212
PvuII CAGCTG 2 cut(s) 299, 697
RsaI GTAC 7 cut(s) 214, 371, 413, 551, 693, 958, 1105
RsaNI GTAC 7 cut(s) 213, 370, 412, 550, 692, 957, 1104
SaqAI TTAA 5 cut(s) 351, 665, 729, 939, 1142
SatI GCNGC 7 cut(s) 297, 306, 524, 650, 789, 1071, 1158
Sau3AI GATC 4 cut(s) 253, 826, 987, 1212
Sau96I GGNCC 4 cut(s) 320, 712, 1223, 1342
ScaI AGTACT 1 cut(s) 551
SchI GAGTC 1 cut(s) 821
SduI GDGCHC 1 cut(s) 291
SfaNI GCATC 6 cut(s) 15, 124, 345, 508, 1042, 1357
SfcI CTRYAG 1 cut(s) 1371
SinI GGWCC 4 cut(s) 320, 712, 1223, 1342
Sse9I AATT 4 cut(s) 662, 749, 1097, 1333
SsiI CCGC 6 cut(s) 119, 430, 649, 800, 1230, 1376
SspMI CTAG 4 cut(s) 602, 792, 816, 1411
StyI CCWWGG 2 cut(s) 822, 980
TaaI ACNGT 4 cut(s) 425, 469, 494, 860
TaiI ACGT 4 cut(s) 658, 799, 1024, 1359
TaqI TCGA 4 cut(s) 9, 143, 197, 1359
TasI AATT 4 cut(s) 662, 749, 1097, 1333
TatI WGTACW 1 cut(s) 549
TauI GCSGC 1 cut(s) 652
TfiI GAWTC 1 cut(s) 79
Tru1I TTAA 5 cut(s) 351, 665, 729, 939, 1142
Tru9I TTAA 5 cut(s) 351, 665, 729, 939, 1142
TseFI GTSAC 2 cut(s) 244, 593
TseI GCWGC 6 cut(s) 296, 305, 523, 788, 1070, 1157
Tsp45I GTSAC 2 cut(s) 244, 593
TspDTI ATGAA 3 cut(s) 1016, 1274, 1293
TspGWI ACGGA 1 cut(s) 112
VneI GTGCAC 1 cut(s) 287
VpaK11BI GGWCC 4 cut(s) 320, 712, 1223, 1342
XapI RAATTY 2 cut(s) 749, 1097
XmiI GTMKAC 1 cut(s) 1296
XspI CTAG 4 cut(s) 602, 792, 816, 1411
ZrmI AGTACT 1 cut(s) 551
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.