Rw1G029580

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0001364
Physical Location & Seq
Forward (+)
57276922 .. 57280016
3095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G029580.1

Sequence Viewer

Length: 1131 bp
ATGGAGGAAGAGAGTCCCTATATCGAGCCTTTCAACAGGTTGGTGAAGCTTGCGGCCAGAGCGTTCTACGATGATGATATAGCAACGTATTCATCGAAAGGGGCTAATTCTCAACTCAAGAGTGGCCGGAGCGTCGAAAATAGAGGAATCGGCGTGGTTGTGCTGGAAGCGCTCTCGAGAAGACAATGGGTAAGAGAAGAAGATTTGGCCAAGGAGTTGAAACTCAACTCGAAGCAACTTCGTCGTACTTTGCAATTTTTCGAGGAGGAAAAACTGGTCACTCGAGACCATAGGAAAGAACGACATGCAAAGGAAGAAGGGGAAGAGAAAATAAAGTTGCACACTTACTCTTATTGCTGTCTGGACTATGCTCAGATGTACGATGTAGTGAGGTACAGACTGCATCGCATGAAGAAAAGACTGAAAGATGCATTGGAGGACAAGAACACAGTACAACAATACTTATGCCACAATTGTGGGAACAGATACAACGCTTTGGATGCAATGCGATTGATTTCGATGGAGGATGATGAGTACTTTCACTGTGAAAGATGCAATGGGAAACTTGTGGTGGTTGACACTAACAATGAGCTAGCTTCTCCACAAGTAGGAGATGAAGAAAGCAATATTTCAAGGAGACAAAGGCGTGAGAAATCGAAAGTCATGCTTCGAAATATGGAGGAACAACTGAAGCCTTTAATGGAACAACTTAATAGGGTTAAGGATTTACCTGTTCCTCAGTTTAGAACACTCCGAGATTGGGAAGCGCGAGCTAAATCTTCTGATGGACATGATTTTGGAGGTACAAAGGGCGACGATATTAAATCGGAATTAGATGATCCTACTACAAGTTTGGGGAAAACTGTGCCACCTTGGATGAACAAGCAAGGGATGAATAACCTTCCAAATCACGAACAACATGGACATTTTTCAGATGACATAAAGGCAAATACTCATGAAGATACTGAGAGCAAGAGTACTTTACAGGAGGAGTATTATAAAGCTTATTATCGTGCTCTAATACAAAGACAACAACAAGAAGAAGCAAATATTCACGAGGTTGGCATGAAGTCAAAATGTGATCAGAAAGAAGAAGACGATGAAGATGATATTGACTGGCAGGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

376

Amino Acids

44.19

Weight (kDa)

5.6

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 52 - 148 6.3e-12 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 999
AccII CGCG 1 cut(s) 769
AciI CCGC 1 cut(s) 53
AclWI GGATC 1 cut(s) 833
AcoI YGGCCR 3 cut(s) 54, 124, 207
AcuI CTGAAG 1 cut(s) 710
AfaI GTAC 7 cut(s) 247, 380, 395, 453, 536, 805, 979
AfeI AGCGCT 1 cut(s) 171
AfiI CCNNNNNNNGG 2 cut(s) 608, 760
AgsI TTSAA 3 cut(s) 34, 220, 633
AjuI GAANNNNNNNTTGG 2 cut(s) 416, 448
AleI CACNNNNGTG 1 cut(s) 474
AluBI AGCT 5 cut(s) 49, 592, 596, 773, 1004
AluI AGCT 5 cut(s) 49, 592, 596, 773, 1004
Alw21I GWGCWC 1 cut(s) 1018
Alw26I GTCTC 2 cut(s) 279, 631
AlwI GGATC 1 cut(s) 833
Ama87I CYCGRG 2 cut(s) 175, 282
Aor51HI AGCGCT 1 cut(s) 171
AoxI GGCC 3 cut(s) 54, 124, 207
AspLEI GCGC 2 cut(s) 172, 769
AsuHPI GGTGA 1 cut(s) 55
AsuII TTCGAA 1 cut(s) 670
AsuNHI GCTAGC 1 cut(s) 592
AvaI CYCGRG 2 cut(s) 175, 282
BalI TGGCCA 1 cut(s) 209
BauI CACGAG 1 cut(s) 1055
BbsI GAAGAC 2 cut(s) 187, 1101
Bbv12I GWGCWC 1 cut(s) 1018
BccI CCATC 2 cut(s) 514, 779
BcgI CGANNNNNNTGC 4 cut(s) 224, 258, 646, 680
BclI TGATCA 1 cut(s) 1081
BcoDI GTCTC 2 cut(s) 279, 631
BfaI CTAG 1 cut(s) 593
BfoI RGCGCY 1 cut(s) 173
BisI GCNGC 1 cut(s) 54
BlsI GCNGC 1 cut(s) 55
BmcAI AGTACT 2 cut(s) 536, 979
BmeT110I CYCGRG 2 cut(s) 175, 282
BmsI GCATC 4 cut(s) 412, 418, 490, 542
BmtI GCTAGC 1 cut(s) 596
BpiI GAAGAC 2 cut(s) 187, 1101
Bpu14I TTCGAA 1 cut(s) 670
BpuEI CTTGAG 1 cut(s) 101
BsaI GGTCTC 1 cut(s) 279
BsaJI CCNNGG 2 cut(s) 210, 872
Bsc4I CCNNNNNNNGG 2 cut(s) 608, 760
Bse1I ACTGG 2 cut(s) 279, 1121
Bse3DI GCAATG 2 cut(s) 510, 562
BseDI CCNNGG 2 cut(s) 210, 872
BseGI GGATG 4 cut(s) 505, 532, 882, 897
BseLI CCNNNNNNNGG 2 cut(s) 608, 760
BseMI GCAATG 2 cut(s) 510, 562
BseMII CTCAG 3 cut(s) 386, 752, 957
BseNI ACTGG 2 cut(s) 279, 1121
BseRI GAGGAG 2 cut(s) 278, 1004
Bsh1236I CGCG 1 cut(s) 769
BshFI GGCC 3 cut(s) 56, 126, 209
BsiHKAI GWGCWC 1 cut(s) 1018
BsiHKCI CYCGRG 2 cut(s) 175, 282
BsiSI CCGG 1 cut(s) 127
BslI CCNNNNNNNGG 2 cut(s) 608, 760
BsmAI GTCTC 2 cut(s) 279, 631
BsnI GGCC 3 cut(s) 56, 126, 209
Bso31I GGTCTC 1 cut(s) 279
BsoBI CYCGRG 2 cut(s) 175, 282
Bsp119I TTCGAA 1 cut(s) 670
Bsp1286I GDGCHC 1 cut(s) 1018
Bsp143I GATC 2 cut(s) 838, 1081
BspACI CCGC 1 cut(s) 53
BspANI GGCC 3 cut(s) 56, 126, 209
BspCNI CTCAG 3 cut(s) 385, 751, 958
BspFNI CGCG 1 cut(s) 769
BspHI TCATGA 1 cut(s) 955
BspOI GCTAGC 1 cut(s) 596
BspPI GGATC 1 cut(s) 833
BspT104I TTCGAA 1 cut(s) 670
BspTNI GGTCTC 1 cut(s) 279
BsrDI GCAATG 2 cut(s) 510, 562
BsrI ACTGG 2 cut(s) 279, 1121
BssECI CCNNGG 2 cut(s) 210, 872
BssMI GATC 2 cut(s) 838, 1081
BssSI CACGAG 1 cut(s) 1055
BssT1I CCWWGG 2 cut(s) 210, 872
Bst2BI CACGAG 1 cut(s) 1055
Bst4CI ACNGT 3 cut(s) 451, 545, 865
Bst6I CTCTTC 2 cut(s) 3, 318
BstBI TTCGAA 1 cut(s) 670
BstC8I GCNNGC 3 cut(s) 51, 594, 771
BstDEI CTNAG 3 cut(s) 372, 738, 966
BstF5I GGATG 4 cut(s) 505, 532, 882, 897
BstFNI CGCG 1 cut(s) 769
BstH2I RGCGCY 1 cut(s) 173
BstHHI GCGC 2 cut(s) 172, 769
BstKTI GATC 2 cut(s) 841, 1084
BstMAI GTCTC 2 cut(s) 279, 631
BstMBI GATC 2 cut(s) 838, 1081
BstMWI GCNNNNNNNGC 3 cut(s) 59, 169, 500
BstNSI RCATGY 1 cut(s) 308
BstUI CGCG 1 cut(s) 769
BstV2I GAAGAC 2 cut(s) 187, 1101
BstXI CCANNNNNNTGG 1 cut(s) 476
BsuRI GGCC 3 cut(s) 56, 126, 209
BtgZI GCGATG 1 cut(s) 389
BtsCI GGATG 4 cut(s) 505, 532, 882, 897
BtsIMutI CAGTG 1 cut(s) 541
Cac8I GCNNGC 3 cut(s) 51, 594, 771
CciI TCATGA 1 cut(s) 955
CfoI GCGC 2 cut(s) 172, 769
CseI GACGC 1 cut(s) 121
Csp6I GTAC 7 cut(s) 246, 379, 394, 452, 535, 804, 978
CviAII CATG 7 cut(s) 305, 409, 664, 791, 920, 956, 1066
CviQI GTAC 7 cut(s) 246, 379, 394, 452, 535, 804, 978
DdeI CTNAG 3 cut(s) 372, 738, 966
DpnI GATC 2 cut(s) 840, 1083
DpnII GATC 2 cut(s) 838, 1081
EaeI YGGCCR 3 cut(s) 54, 124, 207
Eam1104I CTCTTC 2 cut(s) 3, 318
EarI CTCTTC 2 cut(s) 3, 318
Eco130I CCWWGG 2 cut(s) 210, 872
Eco31I GGTCTC 1 cut(s) 279
Eco47III AGCGCT 1 cut(s) 171
Eco57I CTGAAG 1 cut(s) 710
Eco88I CYCGRG 2 cut(s) 175, 282
EcoT14I CCWWGG 2 cut(s) 210, 872
EcoT22I ATGCAT 1 cut(s) 433
ErhI CCWWGG 2 cut(s) 210, 872
FaeI CATG 7 cut(s) 308, 412, 667, 794, 923, 959, 1069
FalI AAGNNNNNCTT 2 cut(s) 651, 683
FatI CATG 7 cut(s) 304, 408, 663, 790, 919, 955, 1065
FbaI TGATCA 1 cut(s) 1081
Fnu4HI GCNGC 1 cut(s) 54
FokI GGATG 4 cut(s) 512, 539, 889, 904
Fsp4HI GCNGC 1 cut(s) 54
FspBI CTAG 1 cut(s) 593
GlaI GCGC 2 cut(s) 171, 768
GluI GCNGC 1 cut(s) 54
HaeII RGCGCY 1 cut(s) 173
HaeIII GGCC 3 cut(s) 56, 126, 209
HapII CCGG 1 cut(s) 127
HgaI GACGC 1 cut(s) 121
HhaI GCGC 2 cut(s) 172, 769
Hin1II CATG 7 cut(s) 308, 412, 667, 794, 923, 959, 1069
Hin6I GCGC 2 cut(s) 170, 767
HinP1I GCGC 2 cut(s) 170, 767
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 2 cut(s) 47, 1002
HinfI GANTC 2 cut(s) 13, 147
HpaII CCGG 1 cut(s) 127
HphI GGTGA 1 cut(s) 55
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 6 cut(s) 375, 755, 784, 829, 934, 1086
Hpy188III TCNNGA 8 cut(s) 118, 175, 177, 284, 362, 911, 956, 1055
Hpy8I GTNNAC 1 cut(s) 577
Hpy99I CGWCG 3 cut(s) 137, 246, 818
HpyAV CCTTC 3 cut(s) 311, 911, 1118
HpyCH4III ACNGT 3 cut(s) 451, 545, 865
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 7 cut(s) 253, 308, 340, 403, 431, 503, 555
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 169, 500
HpyF3I CTNAG 3 cut(s) 372, 738, 966
HpySE526I ACGT 1 cut(s) 86
Hsp92II CATG 7 cut(s) 308, 412, 667, 794, 923, 959, 1069
HspAI GCGC 2 cut(s) 170, 767
Ksp22I TGATCA 1 cut(s) 1081
Kzo9I GATC 2 cut(s) 838, 1081
LmnI GCTCC 1 cut(s) 129
LweI GCATC 4 cut(s) 412, 418, 490, 542
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 86
MaeIII GTNAC 1 cut(s) 277
MalI GATC 2 cut(s) 840, 1083
MboI GATC 2 cut(s) 838, 1081
MfeI CAATTG 1 cut(s) 472
MhlI GDGCHC 1 cut(s) 1018
MlsI TGGCCA 1 cut(s) 209
MluCI AATT 4 cut(s) 106, 254, 472, 830
MluNI TGGCCA 1 cut(s) 209
MlyI GAGTC 1 cut(s) 22
Mox20I TGGCCA 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 433
MscI TGGCCA 1 cut(s) 209
MseI TTAA 4 cut(s) 698, 711, 720, 822
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 209
MspI CCGG 1 cut(s) 127
MunI CAATTG 1 cut(s) 472
MvnI CGCG 1 cut(s) 769
MwoI GCNNNNNNNGC 3 cut(s) 59, 169, 500
NdeII GATC 2 cut(s) 838, 1081
NheI GCTAGC 1 cut(s) 592
NlaIII CATG 7 cut(s) 308, 412, 667, 794, 923, 959, 1069
NmuCI GTSAC 1 cut(s) 277
NsiI ATGCAT 1 cut(s) 433
NspI RCATGY 1 cut(s) 308
NspV TTCGAA 1 cut(s) 670
OliI CACNNNNGTG 1 cut(s) 474
PaeR7I CTCGAG 2 cut(s) 175, 282
PagI TCATGA 1 cut(s) 955
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PfeI GAWTC 1 cut(s) 147
PkrI GCNGC 1 cut(s) 55
PleI GAGTC 1 cut(s) 21
PpsI GAGTC 1 cut(s) 21
PsiI TTATAA 1 cut(s) 999
RsaI GTAC 7 cut(s) 247, 380, 395, 453, 536, 805, 979
RsaNI GTAC 7 cut(s) 246, 379, 394, 452, 535, 804, 978
RseI CAYNNNNRTG 1 cut(s) 474
SaqAI TTAA 4 cut(s) 698, 711, 720, 822
SatI GCNGC 1 cut(s) 54
Sau3AI GATC 2 cut(s) 838, 1081
ScaI AGTACT 2 cut(s) 536, 979
SchI GAGTC 1 cut(s) 22
SduI GDGCHC 1 cut(s) 1018
SfaNI GCATC 4 cut(s) 412, 418, 490, 542
Sfr274I CTCGAG 2 cut(s) 175, 282
SfuI TTCGAA 1 cut(s) 670
SlaI CTCGAG 2 cut(s) 175, 282
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 3 cut(s) 116, 175, 282
SmoI CTYRAG 3 cut(s) 116, 175, 282
Sse9I AATT 4 cut(s) 106, 254, 472, 830
SsiI CCGC 1 cut(s) 53
SspI AATATT 2 cut(s) 628, 1051
SspMI CTAG 1 cut(s) 593
StyI CCWWGG 2 cut(s) 210, 872
TaaI ACNGT 3 cut(s) 451, 545, 865
TaiI ACGT 1 cut(s) 89
TasI AATT 4 cut(s) 106, 254, 472, 830
TatI WGTACW 3 cut(s) 451, 534, 977
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 1 cut(s) 147
Tru1I TTAA 4 cut(s) 698, 711, 720, 822
Tru9I TTAA 4 cut(s) 698, 711, 720, 822
TscAI CASTG 1 cut(s) 548
TseFI GTSAC 1 cut(s) 277
Tsp45I GTSAC 1 cut(s) 277
TspDTI ATGAA 8 cut(s) 81, 425, 630, 893, 908, 972, 1082, 1116
TspRI CASTG 1 cut(s) 548
XceI RCATGY 1 cut(s) 308
XhoI CTCGAG 2 cut(s) 175, 282
XspI CTAG 1 cut(s) 593
ZrmI AGTACT 2 cut(s) 536, 979
Zsp2I ATGCAT 1 cut(s) 433
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.