Rroxscaffold_7G00188140

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001364
Physical Location & Seq
Forward (+)
27635643 .. 27639085
3443 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188140.1

Sequence Viewer

Length: 384 bp
ATGGAGGTCTGTGGGGATGAAGTGGACGGTGAGAAAGTTGAGTTGTGCGGCCTTCCAAGGAGATCAATTCCGAGCTTGGCCACCGTTTTGCAAGCAACTCAACCGGTCTCCGAGCTTGAGAAGCCGCAGCAAGTGCTCCACAACTTGCGAGAGAATGGTGAGTCAAGTGCCATTGATGCCTCCACATCTTCTCATGGGGTGGGGTATGGTGGGACACCCATGCCTTTAGTTAGAGATACTAAGTTGAATATGTTTGGGGACAACGGCAAGGACATCAAATCCGAAGCTGATAATATAGAGGCTGATCAAACAAGGGATGAATCTTACAAGGGAACAATATGGAGAGGTCAGGCAAGTGGAAGCCTCACAAATGCCCGTCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

127

Amino Acids

13.67

Weight (kDa)

4.76

Isoelectric Point (pI)

33.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 48, 125
AcoI YGGCCR 1 cut(s) 78
AgeI ACCGGT 1 cut(s) 103
AgsI TTSAA 1 cut(s) 247
AluBI AGCT 3 cut(s) 75, 115, 287
AluI AGCT 3 cut(s) 75, 115, 287
Alw21I GWGCWC 1 cut(s) 138
Alw26I GTCTC 1 cut(s) 112
AoxI GGCC 2 cut(s) 49, 78
ApeKI GCWGC 1 cut(s) 127
AsiGI ACCGGT 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 41, 170
BalI TGGCCA 1 cut(s) 80
Bbv12I GWGCWC 1 cut(s) 138
BbvI GCAGC 1 cut(s) 139
BceAI ACGGC 1 cut(s) 280
BclI TGATCA 1 cut(s) 304
BcoDI GTCTC 1 cut(s) 112
BfaI CTAG 1 cut(s) 382
BisI GCNGC 3 cut(s) 49, 125, 128
BlsI GCNGC 3 cut(s) 50, 126, 129
BmsI GCATC 1 cut(s) 166
BpuEI CTTGAG 1 cut(s) 137
BsaI GGTCTC 1 cut(s) 112
BsaJI CCNNGG 1 cut(s) 56
BsaWI WCCGGW 1 cut(s) 103
Bse118I RCCGGY 1 cut(s) 103
BseDI CCNNGG 1 cut(s) 56
BseGI GGATG 2 cut(s) 22, 322
BseXI GCAGC 1 cut(s) 139
BshFI GGCC 2 cut(s) 51, 80
BshTI ACCGGT 1 cut(s) 103
BsiHKAI GWGCWC 1 cut(s) 138
BsiSI CCGG 1 cut(s) 104
BslFI GGGAC 2 cut(s) 226, 272
BsmAI GTCTC 1 cut(s) 112
BsmFI GGGAC 2 cut(s) 226, 272
BsnI GGCC 2 cut(s) 51, 80
Bso31I GGTCTC 1 cut(s) 112
Bsp1286I GDGCHC 1 cut(s) 138
Bsp143I GATC 2 cut(s) 62, 304
BspACI CCGC 2 cut(s) 48, 125
BspANI GGCC 2 cut(s) 51, 80
BspTNI GGTCTC 1 cut(s) 112
BsrFI RCCGGY 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 1 cut(s) 56
BssMI GATC 2 cut(s) 62, 304
BssT1I CCWWGG 1 cut(s) 56
Bst4CI ACNGT 2 cut(s) 29, 85
BstAPI GCANNNNNTGC 1 cut(s) 133
BstC8I GCNNGC 1 cut(s) 93
BstDEI CTNAG 1 cut(s) 240
BstF5I GGATG 2 cut(s) 22, 322
BstKTI GATC 2 cut(s) 65, 307
BstMAI GTCTC 1 cut(s) 112
BstMBI GATC 2 cut(s) 62, 304
BstMWI GCNNNNNNNGC 3 cut(s) 121, 133, 176
BstV1I GCAGC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 51, 80
BtsCI GGATG 2 cut(s) 22, 322
Cac8I GCNNGC 1 cut(s) 93
Cfr10I RCCGGY 1 cut(s) 103
CspAI ACCGGT 1 cut(s) 103
CviAII CATG 2 cut(s) 194, 220
CviJI RGCY 8 cut(s) 51, 75, 80, 115, 124, 287, 302, 363
CviKI_1 RGCY 8 cut(s) 51, 75, 80, 115, 124, 287, 302, 363
DdeI CTNAG 1 cut(s) 240
DpnI GATC 2 cut(s) 64, 306
DpnII GATC 2 cut(s) 62, 304
EaeI YGGCCR 1 cut(s) 78
Eco130I CCWWGG 1 cut(s) 56
Eco31I GGTCTC 1 cut(s) 112
EcoT14I CCWWGG 1 cut(s) 56
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 2 cut(s) 197, 223
FaiI YATR 6 cut(s) 195, 207, 221, 251, 296, 340
FaqI GGGAC 2 cut(s) 226, 272
FatI CATG 2 cut(s) 193, 219
FbaI TGATCA 1 cut(s) 304
Fnu4HI GCNGC 3 cut(s) 49, 125, 128
FokI GGATG 2 cut(s) 29, 329
Fsp4HI GCNGC 3 cut(s) 49, 125, 128
FspBI CTAG 1 cut(s) 382
GluI GCNGC 3 cut(s) 49, 125, 128
HaeIII GGCC 2 cut(s) 51, 80
HapII CCGG 1 cut(s) 104
Hin1II CATG 2 cut(s) 197, 223
HinfI GANTC 2 cut(s) 161, 320
HpaII CCGG 1 cut(s) 104
HphI GGTGA 2 cut(s) 41, 170
Hpy166II GTNNAC 1 cut(s) 25
Hpy188I TCNGA 3 cut(s) 72, 112, 283
Hpy8I GTNNAC 1 cut(s) 25
HpyAV CCTTC 1 cut(s) 62
HpyCH4III ACNGT 2 cut(s) 29, 85
HpyCH4V TGCA 1 cut(s) 91
HpyF10VI GCNNNNNNNGC 3 cut(s) 121, 133, 176
HpyF3I CTNAG 1 cut(s) 240
Hsp92II CATG 2 cut(s) 197, 223
Ksp22I TGATCA 1 cut(s) 304
Kzo9I GATC 2 cut(s) 62, 304
LmnI GCTCC 1 cut(s) 141
LpnPI CCDG 2 cut(s) 117, 335
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 1 cut(s) 166
MaeI CTAG 1 cut(s) 382
MalI GATC 2 cut(s) 64, 306
MboI GATC 2 cut(s) 62, 304
MboII GAAGA 1 cut(s) 180
MhlI GDGCHC 1 cut(s) 138
MlsI TGGCCA 1 cut(s) 80
MluCI AATT 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 1 cut(s) 170
MnlI CCTC 4 cut(s) 190, 292, 338, 374
Mox20I TGGCCA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 80
Msp20I TGGCCA 1 cut(s) 80
MspI CCGG 1 cut(s) 104
MwoI GCNNNNNNNGC 3 cut(s) 121, 133, 176
NdeII GATC 2 cut(s) 62, 304
NlaIII CATG 2 cut(s) 197, 223
PfeI GAWTC 1 cut(s) 320
PinAI ACCGGT 1 cut(s) 103
PkrI GCNGC 3 cut(s) 50, 126, 129
PleI GAGTC 1 cut(s) 169
PpsI GAGTC 1 cut(s) 169
SatI GCNGC 3 cut(s) 49, 125, 128
Sau3AI GATC 2 cut(s) 62, 304
SchI GAGTC 1 cut(s) 170
SduI GDGCHC 1 cut(s) 138
SetI ASST 5 cut(s) 9, 77, 117, 289, 349
SfaNI GCATC 1 cut(s) 166
SmlI CTYRAG 1 cut(s) 116
SmoI CTYRAG 1 cut(s) 116
Sse9I AATT 1 cut(s) 66
SsiI CCGC 2 cut(s) 48, 125
SspMI CTAG 1 cut(s) 382
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 29, 85
TasI AATT 1 cut(s) 66
TauI GCSGC 2 cut(s) 51, 127
TfiI GAWTC 1 cut(s) 320
TseI GCWGC 1 cut(s) 127
TspDTI ATGAA 2 cut(s) 33, 333
XspI CTAG 1 cut(s) 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.