Rroxscaffold_7G00188130

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0001364
Physical Location & Seq
Forward (+)
27633574 .. 27635626
2053 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00188130.1

Sequence Viewer

Length: 678 bp
ATGGTTGAGGGTGTTCTACATGGTTTTGTTTACAGTATCTTCGTTGGGACTTTTAGGGATAAAGCTATGTCTCCGAGCTTGAGAAGCCGAGCAAGTGCTCCACAACTTGCAGAGAATGGTGAGTCAAGTGCCATTGATGCCTCCACATCTTCTCATGGGGTGGGGTATGGTGGGACACCCATGCCTTTCGTTAGAGATACTAAGTTTTTCCGATCAAAAGCCCAATCTTTAGAGCCGAGTAAGTTCGGTTCACCTATCCGCTTGTTTCGGATCTTTTCCGGCTTAGCTTGGTCAACGCTCTCCCCTTCCCTCACCGGAGGTAGCTGCTGTCACCGGAGGAGCTCCCGAACCCCAAAGTTCCGAGAACAGACTCAAAATCGATGCCTTGCTCGCTCCTCCGTCCAAGGCCGTGACTCCACGGAGGCGAGCATGCTTCGAGCGGGGTGTCGGTTCTGCTATCGAATCGGTGCTAGGAAAGACAGCATCATTAAGTTCGGACGTGTCTTCATTTACGGCAGTTTCAAGTCTCACGGGGACAATGTTTGGATGAGGGTGTTGCCTCCTAACGCAGCTATGGTACATGTAAGCCAACCGAGGTTCTCAAAACTTGAGGAAAGGCGTCCAGCGGCTACTTCGAAGGAGAAAAGCTACGGAGTAGCTTTGGTTGAAATCGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

225

Amino Acids

24.86

Weight (kDa)

10.31

Isoelectric Point (pI)

54.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 440
AciI CCGC 3 cut(s) 259, 440, 626
AclWI GGATC 1 cut(s) 278
AcyI GRCGYC 1 cut(s) 619
AfaI GTAC 1 cut(s) 579
AflIII ACRYGT 2 cut(s) 499, 580
AgsI TTSAA 2 cut(s) 523, 668
AjiI CACGTC 1 cut(s) 500
AluBI AGCT 8 cut(s) 65, 78, 287, 324, 342, 572, 648, 659
AluI AGCT 8 cut(s) 65, 78, 287, 324, 342, 572, 648, 659
Alw21I GWGCWC 2 cut(s) 100, 344
Alw26I GTCTC 2 cut(s) 75, 531
AlwI GGATC 1 cut(s) 278
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 2 cut(s) 324, 569
AsuHPI GGTGA 4 cut(s) 131, 243, 304, 323
AsuII TTCGAA 1 cut(s) 635
BanII GRGCYC 1 cut(s) 344
BbsI GAAGAC 1 cut(s) 496
Bbv12I GWGCWC 2 cut(s) 100, 344
BbvI GCAGC 2 cut(s) 311, 581
BceAI ACGGC 2 cut(s) 393, 529
BcoDI GTCTC 2 cut(s) 75, 531
BfaI CTAG 1 cut(s) 471
BisI GCNGC 3 cut(s) 325, 570, 627
BlpI GCTNAGC 1 cut(s) 283
BlsI GCNGC 3 cut(s) 326, 571, 628
BmgBI CACGTC 1 cut(s) 500
BmsI GCATC 3 cut(s) 127, 371, 492
BpiI GAAGAC 1 cut(s) 496
Bpu1102I GCTNAGC 1 cut(s) 283
Bpu14I TTCGAA 1 cut(s) 635
BpuEI CTTGAG 2 cut(s) 100, 629
Bsa29I ATCGAT 2 cut(s) 379, 672
BsaHI GRCGYC 1 cut(s) 619
BsaJI CCNNGG 3 cut(s) 403, 417, 593
BsaWI WCCGGW 2 cut(s) 314, 333
BseCI ATCGAT 2 cut(s) 379, 672
BseDI CCNNGG 3 cut(s) 403, 417, 593
BseGI GGATG 1 cut(s) 552
BseRI GAGGAG 2 cut(s) 352, 385
BseXI GCAGC 2 cut(s) 311, 581
BshFI GGCC 1 cut(s) 408
BshVI ATCGAT 2 cut(s) 379, 672
BsiHKAI GWGCWC 2 cut(s) 100, 344
BsiSI CCGG 3 cut(s) 279, 315, 334
BslFI GGGAC 3 cut(s) 61, 187, 548
BsmAI GTCTC 2 cut(s) 75, 531
BsmFI GGGAC 3 cut(s) 61, 187, 548
BsnI GGCC 1 cut(s) 408
Bsp119I TTCGAA 1 cut(s) 635
Bsp1286I GDGCHC 2 cut(s) 100, 344
Bsp143I GATC 2 cut(s) 212, 270
Bsp1720I GCTNAGC 1 cut(s) 283
BspACI CCGC 3 cut(s) 259, 440, 626
BspANI GGCC 1 cut(s) 408
BspDI ATCGAT 2 cut(s) 379, 672
BspPI GGATC 1 cut(s) 278
BspT104I TTCGAA 1 cut(s) 635
BsrBI CCGCTC 1 cut(s) 440
BssECI CCNNGG 3 cut(s) 403, 417, 593
BssMI GATC 2 cut(s) 212, 270
BssNI GRCGYC 1 cut(s) 619
BssT1I CCWWGG 1 cut(s) 403
Bst4CI ACNGT 1 cut(s) 35
BstACI GRCGYC 1 cut(s) 619
BstBI TTCGAA 1 cut(s) 635
BstC8I GCNNGC 3 cut(s) 391, 427, 431
BstDEI CTNAG 2 cut(s) 201, 283
BstDSI CCRYGG 1 cut(s) 417
BstF5I GGATG 1 cut(s) 552
BstKTI GATC 2 cut(s) 215, 273
BstMAI GTCTC 2 cut(s) 75, 531
BstMBI GATC 2 cut(s) 212, 270
BstMWI GCNNNNNNNGC 3 cut(s) 84, 137, 390
BstNSI RCATGY 2 cut(s) 433, 584
BstV1I GCAGC 2 cut(s) 311, 581
BstV2I GAAGAC 1 cut(s) 496
BstX2I RGATCY 1 cut(s) 270
BstYI RGATCY 1 cut(s) 270
Bsu15I ATCGAT 2 cut(s) 379, 672
BsuRI GGCC 1 cut(s) 408
BsuTUI ATCGAT 2 cut(s) 379, 672
BtgI CCRYGG 1 cut(s) 417
BtrI CACGTC 1 cut(s) 500
BtsCI GGATG 1 cut(s) 552
Cac8I GCNNGC 3 cut(s) 391, 427, 431
ClaI ATCGAT 2 cut(s) 379, 672
CseI GACGC 1 cut(s) 608
Csp6I GTAC 1 cut(s) 578
CviAII CATG 5 cut(s) 20, 155, 181, 430, 581
CviQI GTAC 1 cut(s) 578
DdeI CTNAG 2 cut(s) 201, 283
DpnI GATC 2 cut(s) 214, 272
DpnII GATC 2 cut(s) 212, 270
Ecl136II GAGCTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 403
Eco24I GRGCYC 1 cut(s) 344
Eco53kI GAGCTC 1 cut(s) 342
EcoICRI GAGCTC 1 cut(s) 342
EcoT14I CCWWGG 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 344
ErhI CCWWGG 1 cut(s) 403
FaeI CATG 5 cut(s) 23, 158, 184, 433, 584
FaiI YATR 8 cut(s) 21, 68, 156, 168, 182, 431, 575, 582
FaqI GGGAC 3 cut(s) 61, 187, 548
FatI CATG 5 cut(s) 19, 154, 180, 429, 580
FauI CCCGC 1 cut(s) 433
Fnu4HI GCNGC 3 cut(s) 325, 570, 627
FokI GGATG 1 cut(s) 559
FriOI GRGCYC 1 cut(s) 344
Fsp4HI GCNGC 3 cut(s) 325, 570, 627
FspBI CTAG 1 cut(s) 471
GluI GCNGC 3 cut(s) 325, 570, 627
HaeIII GGCC 1 cut(s) 408
HapII CCGG 3 cut(s) 279, 315, 334
HgaI GACGC 1 cut(s) 608
Hin1I GRCGYC 1 cut(s) 619
Hin1II CATG 5 cut(s) 23, 158, 184, 433, 584
HincII GTYRAC 1 cut(s) 294
HindII GTYRAC 1 cut(s) 294
HinfI GANTC 4 cut(s) 122, 370, 413, 462
HpaII CCGG 3 cut(s) 279, 315, 334
HphI GGTGA 4 cut(s) 131, 243, 304, 323
Hpy166II GTNNAC 3 cut(s) 31, 251, 294
Hpy188I TCNGA 5 cut(s) 75, 212, 270, 362, 497
Hpy188III TCNNGA 1 cut(s) 345
Hpy8I GTNNAC 3 cut(s) 31, 251, 294
HpyAV CCTTC 2 cut(s) 315, 631
HpyCH4III ACNGT 1 cut(s) 35
HpyCH4IV ACGT 1 cut(s) 499
HpyCH4V TGCA 1 cut(s) 110
HpyF10VI GCNNNNNNNGC 3 cut(s) 84, 137, 390
HpyF3I CTNAG 2 cut(s) 201, 283
HpySE526I ACGT 1 cut(s) 499
Hsp92I GRCGYC 1 cut(s) 619
Hsp92II CATG 5 cut(s) 23, 158, 184, 433, 584
Kzo9I GATC 2 cut(s) 212, 270
LmnI GCTCC 4 cut(s) 103, 339, 347, 398
LpnPI CCDG 4 cut(s) 292, 328, 347, 636
Lsp1109I GCAGC 2 cut(s) 311, 581
LweI GCATC 3 cut(s) 127, 371, 492
MaeI CTAG 1 cut(s) 471
MaeII ACGT 1 cut(s) 499
MaeIII GTNAC 2 cut(s) 329, 410
MalI GATC 2 cut(s) 214, 272
MbiI CCGCTC 1 cut(s) 440
MboI GATC 2 cut(s) 212, 270
MboII GAAGA 3 cut(s) 31, 141, 496
MflI RGATCY 1 cut(s) 270
MhlI GDGCHC 2 cut(s) 100, 344
MlyI GAGTC 3 cut(s) 131, 364, 407
MseI TTAA 1 cut(s) 489
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 3 cut(s) 279, 315, 334
MwoI GCNNNNNNNGC 3 cut(s) 84, 137, 390
NdeII GATC 2 cut(s) 212, 270
NlaIII CATG 5 cut(s) 23, 158, 184, 433, 584
NmeAIII GCCGAG 2 cut(s) 113, 261
NmuCI GTSAC 2 cut(s) 329, 410
NspI RCATGY 2 cut(s) 433, 584
NspV TTCGAA 1 cut(s) 635
PaeI GCATGC 1 cut(s) 433
PciI ACATGT 1 cut(s) 580
PfeI GAWTC 1 cut(s) 462
PkrI GCNGC 3 cut(s) 326, 571, 628
PleI GAGTC 3 cut(s) 130, 364, 407
PpsI GAGTC 3 cut(s) 130, 364, 407
PscI ACATGT 1 cut(s) 580
Psp124BI GAGCTC 1 cut(s) 344
PsrI GAACNNNNNNTAC 2 cut(s) 232, 264
PsuI RGATCY 1 cut(s) 270
RsaI GTAC 1 cut(s) 579
RsaNI GTAC 1 cut(s) 578
SacI GAGCTC 1 cut(s) 344
SaqAI TTAA 1 cut(s) 489
SatI GCNGC 3 cut(s) 325, 570, 627
Sau3AI GATC 2 cut(s) 212, 270
SchI GAGTC 3 cut(s) 131, 364, 407
SduI GDGCHC 2 cut(s) 100, 344
SfaNI GCATC 3 cut(s) 127, 371, 492
SfuI TTCGAA 1 cut(s) 635
SmlI CTYRAG 2 cut(s) 79, 608
SmoI CTYRAG 2 cut(s) 79, 608
SphI GCATGC 1 cut(s) 433
SsiI CCGC 3 cut(s) 259, 440, 626
SspMI CTAG 1 cut(s) 471
SstI GAGCTC 1 cut(s) 344
StyI CCWWGG 1 cut(s) 403
TaaI ACNGT 1 cut(s) 35
TaiI ACGT 1 cut(s) 502
TaqI TCGA 5 cut(s) 379, 436, 460, 635, 672
TauI GCSGC 1 cut(s) 629
TfiI GAWTC 1 cut(s) 462
Tru1I TTAA 1 cut(s) 489
Tru9I TTAA 1 cut(s) 489
TseFI GTSAC 2 cut(s) 329, 410
TseI GCWGC 2 cut(s) 324, 569
Tsp45I GTSAC 2 cut(s) 329, 410
TspDTI ATGAA 1 cut(s) 496
TspGWI ACGGA 3 cut(s) 388, 434, 666
XceI RCATGY 2 cut(s) 433, 584
XspI CTAG 1 cut(s) 471
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.