AT4G20340

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
4
OG0001364
Physical Location & Seq
Forward (+)
10984618 .. 10988008
3391 bp
Loading structure...
UTR
Exon/CDS
Intron
AT4G20340.2

Sequence Viewer

Length: 1428 bp
ATGGACAAATCAATCACGGTGGTGCGGAAAACCGTTGTGCTCGAGCCGTTTGTCAAGCTGGTGAGGCTTTTAGTAAGGATTTTCTATGACAATTATACTCCGGAAAGCGATAATCAGCAGAAATCTGTGAAAAATGTTAAAGGTAGTGCTGTTATCGTGCTGGACGCGCTTACGAGGCGGCAATGGGTTAGAGAAGAAGACTTGGCAAAAGAGGTGAAGCGGAATGCCAAGGAGCTTCGAAAATTAATAAGACATTTTGAAGAACAAAAATTTGTCATGCGTTACCACAGGAAAGAGACTGCAAAGCGTGCGAAGATGTATAGTTATGCAGTGGGTGGTACAACTGATGGCCGAGCAGAGGACAATGTTAAGTTCCACACGCACTCGTATTGCTGTCTTGATTATGCACAGATATATGATATTGTTCGTTATAAACTGCACCGGCTCAAGAAAAAGTTCAAAGATGAACTAGAAGACAGAAATACTGTTCAGGAGTATGGCTGTCCTAACTGCAAAAGAAAATATAATGCACTGGATGCCCTGAGATTAATTTCTATGGAAGACGATTCTTTTCATTGTGAAAATTGCAATGGTGAACTTGTTATGGAATGTAACAAGCTAATTTCCGAAGAAGTAGTAGATAGAGGTGACAATGCAAGGAGACGGCAGCGGGAGAAAGTAAAAGTTTGGCTTCAGGATTTGGAGGGTGAACTGAAACCCCTGATGGAACTAATAAATAGAGTAAAAGACCTACCGTTCCCTGCCTTTGAACCTTTTCCGGCATGGGAAGCTCGTGCAGCTAAGGCTGCTCGTGAAAATGGTGATTTTAACCCTGATGATCCTTCAAGGTCTTTGGGCGGATATGGTTCAACACCAATGCCATTTCTGGGAGAGACAAAGGTTGAGGTTAACCTTGGTGAAGGAAATGAAGATGTCACATCTACAGGTGGAGATTCTAGCCTGAAAATGTTGCCTCCATGGATGATCAAGCAAGGCATGAAGCTGACCGAGGAACAAAGAGGAGAGATGAGACAGGAAGCAAATGTTGATGGAGAAGCAGCAAAACTTTCAGATGACAAGAAATCAGTCATGGAAAATGGCGATGATAATAAAGATTTGAAGGATGAGTATCTCAAAGCTTACTACGCCGCTATAATGGAGCAGCAAAAGCTTGCTGCAAAGCTGAATGAGCAAGAATCCGCAGGCGAATCAACAACCACTGACATTGAATCGGCTACTACATACTCAGATCGTCAAGTTGGTATGAAGTCCAAACGCGAAGAGGAAGAAGAAGATGTTGAATGGGAAGAAGGAGCTTCTGTTGCAGCAAATGGAAACTACAAGGTGGATTTGAATGTGGAAGCAGAAGAGGCAGAAGAAAAAGAAGATGGAGACGAAGATGACGACATCGACTGGGAAGAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

475

Amino Acids

54.58

Weight (kDa)

4.95

Isoelectric Point (pI)

45.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 45 - 111 1.2e-07 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 432
AccII CGCG 2 cut(s) 167, 1278
AccIII TCCGGA 1 cut(s) 100
AciI CCGC 7 cut(s) 25, 178, 220, 670, 858, 1149, 1200
AclWI GGATC 1 cut(s) 833
AcoI YGGCCR 1 cut(s) 349
AcsI RAATTY 1 cut(s) 269
AcuI CTGAAG 1 cut(s) 677
AfaI GTAC 1 cut(s) 340
AfiI CCNNNNNNNGG 2 cut(s) 358, 887
AgsI TTSAA 9 cut(s) 260, 460, 770, 846, 870, 1120, 1229, 1301, 1354
AleI CACNNNNGTG 1 cut(s) 20
Alw21I GWGCWC 1 cut(s) 42
Alw26I GTCTC 5 cut(s) 290, 655, 887, 1024, 1386
AlwI GGATC 1 cut(s) 833
Ama87I CYCGRG 1 cut(s) 41
Aor13HI TCCGGA 1 cut(s) 100
AoxI GGCC 1 cut(s) 349
ApeKI GCWGC 7 cut(s) 667, 797, 806, 1058, 1162, 1175, 1325
ApoI RAATTY 1 cut(s) 269
AseI ATTAAT 2 cut(s) 245, 548
Asp700I GAANNNNTTC 2 cut(s) 455, 774
AspLEI GCGC 1 cut(s) 169
AsuHPI GGTGA 7 cut(s) 73, 226, 605, 659, 719, 833, 929
AsuII TTCGAA 1 cut(s) 238
AvaI CYCGRG 1 cut(s) 41
BauI CACGAG 2 cut(s) 792, 810
BbsI GAAGAC 3 cut(s) 204, 480, 567
Bbv12I GWGCWC 1 cut(s) 42
BbvI GCAGC 7 cut(s) 679, 793, 809, 1070, 1162, 1174, 1337
BccI CCATC 4 cut(s) 341, 718, 1043, 1382
BceAI ACGGC 2 cut(s) 31, 680
BclI TGATCA 1 cut(s) 984
BcoDI GTCTC 5 cut(s) 290, 655, 887, 1024, 1386
BfaI CTAG 2 cut(s) 470, 957
BfmI CTRYAG 1 cut(s) 942
BisI GCNGC 9 cut(s) 179, 668, 798, 807, 1059, 1149, 1163, 1176, 1326
BlsI GCNGC 9 cut(s) 180, 669, 799, 808, 1060, 1150, 1164, 1177, 1327
BmeT110I CYCGRG 1 cut(s) 41
BmrI ACTGGG 1 cut(s) 1423
BmsI GCATC 1 cut(s) 526
BmuI ACTGGG 1 cut(s) 1423
BpiI GAAGAC 3 cut(s) 204, 480, 567
Bpu10I CCTNAGC 1 cut(s) 801
Bpu14I TTCGAA 1 cut(s) 238
BpuEI CTTGAG 1 cut(s) 431
BsaBI GATNNNNATC 1 cut(s) 1128
BsaJI CCNNGG 4 cut(s) 228, 913, 977, 1008
BsaWI WCCGGW 1 cut(s) 100
BsaXI ACNNNNNCTCC 2 cut(s) 1305, 1335
Bsc4I CCNNNNNNNGG 2 cut(s) 358, 887
Bse118I RCCGGY 1 cut(s) 441
Bse1I ACTGG 2 cut(s) 537, 1418
Bse3DI GCAATG 2 cut(s) 188, 595
Bse8I GATNNNNATC 1 cut(s) 1128
BseAI TCCGGA 1 cut(s) 100
BseDI CCNNGG 4 cut(s) 228, 913, 977, 1008
BseGI GGATG 3 cut(s) 541, 987, 1129
BseJI GATNNNNATC 1 cut(s) 1128
BseLI CCNNNNNNNGG 2 cut(s) 358, 887
BseMI GCAATG 2 cut(s) 188, 595
BseMII CTCAG 2 cut(s) 533, 1260
BseNI ACTGG 2 cut(s) 537, 1418
BseRI GAGGAG 1 cut(s) 1035
BseXI GCAGC 7 cut(s) 679, 793, 809, 1070, 1162, 1174, 1337
BsgI GTGCAG 2 cut(s) 422, 816
Bsh1236I CGCG 2 cut(s) 167, 1278
BshFI GGCC 1 cut(s) 351
BsiHKAI GWGCWC 1 cut(s) 42
BsiHKCI CYCGRG 1 cut(s) 41
BsiSI CCGG 3 cut(s) 101, 442, 779
BslI CCNNNNNNNGG 2 cut(s) 358, 887
BsmAI GTCTC 5 cut(s) 290, 655, 887, 1024, 1386
BsmBI CGTCTC 2 cut(s) 655, 1386
BsmI GAATGC 1 cut(s) 229
BsnI GGCC 1 cut(s) 351
BsoBI CYCGRG 1 cut(s) 41
Bsp119I TTCGAA 1 cut(s) 238
Bsp1286I GDGCHC 1 cut(s) 42
Bsp13I TCCGGA 1 cut(s) 100
Bsp143I GATC 3 cut(s) 838, 984, 1249
Bsp19I CCATGG 1 cut(s) 977
BspACI CCGC 7 cut(s) 25, 178, 220, 670, 858, 1149, 1200
BspANI GGCC 1 cut(s) 351
BspCNI CTCAG 2 cut(s) 534, 1259
BspEI TCCGGA 1 cut(s) 100
BspFNI CGCG 2 cut(s) 167, 1278
BspPI GGATC 1 cut(s) 833
BspT104I TTCGAA 1 cut(s) 238
BsrDI GCAATG 2 cut(s) 188, 595
BsrFI RCCGGY 1 cut(s) 441
BsrI ACTGG 2 cut(s) 537, 1418
BssAI RCCGGY 1 cut(s) 441
BssECI CCNNGG 4 cut(s) 228, 913, 977, 1008
BssMI GATC 3 cut(s) 838, 984, 1249
BssSI CACGAG 2 cut(s) 792, 810
BssT1I CCWWGG 3 cut(s) 228, 913, 977
Bst2BI CACGAG 2 cut(s) 792, 810
Bst4CI ACNGT 4 cut(s) 19, 34, 487, 756
Bst6I CTCTTC 2 cut(s) 1275, 1362
BstAPI GCANNNNNTGC 2 cut(s) 308, 536
BstBI TTCGAA 1 cut(s) 238
BstC8I GCNNGC 3 cut(s) 309, 1173, 1204
BstDEI CTNAG 3 cut(s) 542, 801, 1246
BstDSI CCRYGG 1 cut(s) 977
BstF5I GGATG 3 cut(s) 541, 987, 1129
BstFNI CGCG 2 cut(s) 167, 1278
BstHHI GCGC 1 cut(s) 169
BstKTI GATC 3 cut(s) 841, 987, 1252
BstMAI GTCTC 5 cut(s) 290, 655, 887, 1024, 1386
BstMBI GATC 3 cut(s) 838, 984, 1249
BstSFI CTRYAG 1 cut(s) 942
BstUI CGCG 2 cut(s) 167, 1278
BstV1I GCAGC 7 cut(s) 679, 793, 809, 1070, 1162, 1174, 1337
BstV2I GAAGAC 3 cut(s) 204, 480, 567
BsuRI GGCC 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 977
BtgZI GCGATG 1 cut(s) 1116
BtsCI GGATG 3 cut(s) 541, 987, 1129
BtsI GCAGTG 1 cut(s) 336
BtsIMutI CAGTG 3 cut(s) 336, 530, 1218
Cac8I GCNNGC 3 cut(s) 309, 1173, 1204
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 441
CseI GACGC 1 cut(s) 173
Csp6I GTAC 1 cut(s) 339
CspCI CAANNNNNGTGG 3 cut(s) 35, 1207, 1242
CviAII CATG 5 cut(s) 277, 783, 978, 997, 1090
CviQI GTAC 1 cut(s) 339
DdeI CTNAG 3 cut(s) 542, 801, 1246
DpnI GATC 3 cut(s) 840, 986, 1251
DpnII GATC 3 cut(s) 838, 984, 1249
EaeI YGGCCR 1 cut(s) 349
Eam1104I CTCTTC 2 cut(s) 1275, 1362
EarI CTCTTC 2 cut(s) 1275, 1362
EciI GGCGGA 1 cut(s) 873
Eco130I CCWWGG 3 cut(s) 228, 913, 977
Eco57I CTGAAG 1 cut(s) 677
Eco88I CYCGRG 1 cut(s) 41
EcoT14I CCWWGG 3 cut(s) 228, 913, 977
ErhI CCWWGG 3 cut(s) 228, 913, 977
Esp3I CGTCTC 2 cut(s) 655, 1386
FaeI CATG 5 cut(s) 280, 786, 981, 1000, 1093
FalI AAGNNNNNCTT 2 cut(s) 675, 707
FatI CATG 5 cut(s) 276, 782, 977, 996, 1089
FauI CCCGC 1 cut(s) 663
FbaI TGATCA 1 cut(s) 984
Fnu4HI GCNGC 9 cut(s) 179, 668, 798, 807, 1059, 1149, 1163, 1176, 1326
FokI GGATG 3 cut(s) 548, 994, 1136
Fsp4HI GCNGC 9 cut(s) 179, 668, 798, 807, 1059, 1149, 1163, 1176, 1326
FspBI CTAG 2 cut(s) 470, 957
GlaI GCGC 1 cut(s) 168
GluI GCNGC 9 cut(s) 179, 668, 798, 807, 1059, 1149, 1163, 1176, 1326
HaeIII GGCC 1 cut(s) 351
HapII CCGG 3 cut(s) 101, 442, 779
HgaI GACGC 1 cut(s) 173
HhaI GCGC 1 cut(s) 169
Hin1II CATG 5 cut(s) 280, 786, 981, 1000, 1093
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 2 cut(s) 1137, 1169
HinfI GANTC 5 cut(s) 566, 953, 1196, 1208, 1229
HpaI GTTAAC 1 cut(s) 910
HpaII CCGG 3 cut(s) 101, 442, 779
HphI GGTGA 7 cut(s) 73, 226, 605, 659, 719, 833, 929
Hpy166II GTNNAC 3 cut(s) 596, 710, 910
Hpy188I TCNGA 3 cut(s) 628, 1072, 1249
Hpy188III TCNNGA 6 cut(s) 101, 398, 448, 491, 695, 812
Hpy8I GTNNAC 3 cut(s) 596, 710, 910
HpyAV CCTTC 5 cut(s) 852, 914, 1114, 1304, 1415
HpyCH4III ACNGT 4 cut(s) 19, 34, 487, 756
HpyF3I CTNAG 3 cut(s) 542, 801, 1246
Hsp92II CATG 5 cut(s) 280, 786, 981, 1000, 1093
HspAI GCGC 1 cut(s) 167
Kpn2I TCCGGA 1 cut(s) 100
Ksp22I TGATCA 1 cut(s) 984
KspAI GTTAAC 1 cut(s) 910
Kzo9I GATC 3 cut(s) 838, 984, 1249
LmnI GCTCC 3 cut(s) 232, 1159, 1313
Lsp1109I GCAGC 7 cut(s) 679, 793, 809, 1070, 1162, 1174, 1337
LweI GCATC 1 cut(s) 526
MaeI CTAG 2 cut(s) 470, 957
MaeIII GTNAC 4 cut(s) 281, 611, 647, 934
MalI GATC 3 cut(s) 840, 986, 1251
MboI GATC 3 cut(s) 838, 984, 1249
MhlI GDGCHC 1 cut(s) 42
MluCI AATT 6 cut(s) 91, 242, 269, 549, 583, 621
MroI TCCGGA 1 cut(s) 100
MroXI GAANNNNTTC 2 cut(s) 455, 774
MseI TTAA 6 cut(s) 138, 245, 369, 548, 828, 909
MslI CAYNNNNRTG 1 cut(s) 20
MspA1I CMGCKG 1 cut(s) 670
MspI CCGG 3 cut(s) 101, 442, 779
Mva1269I GAATGC 1 cut(s) 229
MvnI CGCG 2 cut(s) 167, 1278
NcoI CCATGG 1 cut(s) 977
NdeII GATC 3 cut(s) 838, 984, 1249
NlaIII CATG 5 cut(s) 280, 786, 981, 1000, 1093
NmeAIII GCCGAG 1 cut(s) 377
NmuCI GTSAC 2 cut(s) 647, 934
NspV TTCGAA 1 cut(s) 238
OliI CACNNNNGTG 1 cut(s) 20
PaeR7I CTCGAG 1 cut(s) 41
PcsI WCGNNNNNNNCGW 1 cut(s) 1401
PctI GAATGC 1 cut(s) 229
PdmI GAANNNNTTC 2 cut(s) 455, 774
PfeI GAWTC 5 cut(s) 566, 953, 1196, 1208, 1229
PkrI GCNGC 9 cut(s) 180, 669, 799, 808, 1060, 1150, 1164, 1177, 1327
PshBI ATTAAT 2 cut(s) 245, 548
PsiI TTATAA 1 cut(s) 432
PspXI VCTCGAGB 1 cut(s) 41
RsaI GTAC 1 cut(s) 340
RsaNI GTAC 1 cut(s) 339
RseI CAYNNNNRTG 1 cut(s) 20
SaqAI TTAA 6 cut(s) 138, 245, 369, 548, 828, 909
SatI GCNGC 9 cut(s) 179, 668, 798, 807, 1059, 1149, 1163, 1176, 1326
Sau3AI GATC 3 cut(s) 838, 984, 1249
SduI GDGCHC 1 cut(s) 42
SfaNI GCATC 1 cut(s) 526
SfcI CTRYAG 1 cut(s) 942
Sfr274I CTCGAG 1 cut(s) 41
SfuI TTCGAA 1 cut(s) 238
SlaI CTCGAG 1 cut(s) 41
SmiMI CAYNNNNRTG 1 cut(s) 20
SmlI CTYRAG 2 cut(s) 41, 446
SmoI CTYRAG 2 cut(s) 41, 446
Sse9I AATT 6 cut(s) 91, 242, 269, 549, 583, 621
SsiI CCGC 7 cut(s) 25, 178, 220, 670, 858, 1149, 1200
SspMI CTAG 2 cut(s) 470, 957
StyI CCWWGG 3 cut(s) 228, 913, 977
TaaI ACNGT 4 cut(s) 19, 34, 487, 756
TaqI TCGA 3 cut(s) 42, 238, 1410
TaqII GACCGA 1 cut(s) 1022
TasI AATT 6 cut(s) 91, 242, 269, 549, 583, 621
TauI GCSGC 2 cut(s) 181, 1151
TfiI GAWTC 5 cut(s) 566, 953, 1196, 1208, 1229
Tru1I TTAA 6 cut(s) 138, 245, 369, 548, 828, 909
Tru9I TTAA 6 cut(s) 138, 245, 369, 548, 828, 909
TscAI CASTG 3 cut(s) 336, 537, 1225
TseFI GTSAC 2 cut(s) 647, 934
TseI GCWGC 7 cut(s) 667, 797, 806, 1058, 1162, 1175, 1325
Tsp45I GTSAC 2 cut(s) 647, 934
TspDTI ATGAA 5 cut(s) 480, 563, 942, 1013, 1280
TspRI CASTG 3 cut(s) 336, 537, 1225
VspI ATTAAT 2 cut(s) 245, 548
XapI RAATTY 1 cut(s) 269
XhoI CTCGAG 1 cut(s) 41
XmnI GAANNNNTTC 2 cut(s) 455, 774
XspI CTAG 2 cut(s) 470, 957
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.