FvH4_5g12750

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0001364
Physical Location & Seq
Forward (+)
7194169 .. 7202751
8583 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g12750.t1

Sequence Viewer

Length: 1506 bp
ATGGTAACCGAAGAGTATATAAAGGCCGGCAGTTTCATAAACACAACAGAATCCCCAGAATCTCCCTCGCTCTCTGGCAGAAGGGGAAGTGACCCACTACGTAAAACCCATTTCGAAAACGAAATTGGCATTGCCTCCAAAACCATGAGCATCGACGTTTTCAACAGGTTCGTGAGGCTTGCCGCCAGAGCTTTCTACGATGACATCACGCCGGAAGGTGATAATAAGCAGCCCAGGACTGGCCGGATTAGCGACAATAGGGGAATCGCCGTCGTGGTGCTCGACGCGCTCTCAAGGCGACAATGGGTGAGAGAAGAAGATTTGGCAAAGGACTTGAAACTGCACCCTAAGCAGCTTCGTTGGGTTTTGCAATTTTTGATGGAGGAAAGACTGGTGATCAGAGAACATAGGAAGGAGACAGCTAAAGGTGTGAAGATTTTCAGTGCTGCAGTTGCTGCCACAGCCAATGGGCAACAACATAGAAAAGAAGGCGATGAGAAAACTAAGCTGCAGACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCGTATGAGGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCCAACTGTGGGAAAAGATACAATGCTTTGGATGCGCTACAACTCCTTTCCTTGGAGGATGAGTACTTCCACTGCGAGAGTTGCAATGGGGAGCTTGTGGCTGAGAGTGATAAGCTAGCTTCTCAAGAAGTAGGAGATGGAGATAGCAATGCAAGGAGGCAAAGGCGGGAAAAATTAAGAGACATGCTTCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGGGTGAAAGACTTACCTCCTCCTGAATTTGGCACTCTCCAAGCTTGGGAAGCGCGAGCACATGCTCAACGACGTGCCGAGAATGGTGAATCCAGTGGTGATCCCTCCAAATCTTCACATGGGTATGGTGGAACACCCATGCCTTTTCTTGGAGATACAAAAGTTGAAGTTGATTTTGATAATGAAGGCAAGGGTAACGATGTCAAATCAGAATCTGATACTACAGGCCAAAAAGTTTTGCCACCTTGGATGATAAAGCAAGGGATGAACCTTACAAAGGAACAGCGTGGAGAGGTAAAGCAGGAGGCAAAGATGGATGGTACTGTAGCGGCTCCAGAGTATTCAGATGACAAAAAGGCAAATGCTGAAGATAATGAGAAGGCTTTCCAGGATGAGTATCTCAAAGCTTATTATGCTGCTATAATGAAAAGACAAGAAGAAGAATCAGCAAAGAGTCGTCAGGTTGGCATGAAGTCAAAACGTGATCCAAATGAAGGAGATGAAGCAGAAGAAAACATTTGGGAAGAGGCTCCAGTTGCAGGTGATGCCAACGGAAATTACAAGGTCAATGACTTGAATGTCGAAGCCGATGCTACTGCAGAAGATGAGGATGACATAGACTGGGAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

502

Amino Acids

56.95

Weight (kDa)

5.12

Isoelectric Point (pI)

47.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 90 - 144 2.6e-07 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1403
AasI GACNNNNNNGTC 1 cut(s) 1451
Acc36I ACCTGC 1 cut(s) 1403
AccII CGCG 2 cut(s) 287, 928
AciI CCGC 3 cut(s) 183, 805, 1202
AclWI GGATC 2 cut(s) 968, 1352
AcoI YGGCCR 1 cut(s) 241
AcsI RAATTY 1 cut(s) 899
AcuI CTGAAG 2 cut(s) 869, 1260
AfaI GTAC 4 cut(s) 566, 704, 843, 1195
AgsI TTSAA 5 cut(s) 163, 337, 830, 1040, 1450
AjiI CACGTC 1 cut(s) 947
AjnI CCWGG 2 cut(s) 233, 1260
AjuI GAANNNNNNNTTGG 4 cut(s) 108, 140, 587, 619
Alw21I GWGCWC 2 cut(s) 282, 934
Alw26I GTCTC 2 cut(s) 410, 813
AlwI GGATC 2 cut(s) 968, 1352
AlwNI CAGNNNCTG 2 cut(s) 455, 1088
AoxI GGCC 3 cut(s) 24, 241, 1099
ApeKI GCWGC 6 cut(s) 229, 352, 446, 455, 508, 1289
ApoI RAATTY 1 cut(s) 899
AseI ATTAAT 1 cut(s) 870
Asp700I GAANNNNTTC 2 cut(s) 437, 1256
AspLEI GCGC 3 cut(s) 289, 676, 928
AsuHPI GGTGA 7 cut(s) 230, 319, 406, 889, 971, 983, 1427
AsuII TTCGAA 1 cut(s) 114
AsuNHI GCTAGC 1 cut(s) 754
BaeI ACNNNNGTAYC 2 cut(s) 1020, 1053
Bbv12I GWGCWC 2 cut(s) 282, 934
BbvI GCAGC 6 cut(s) 241, 364, 433, 442, 495, 1276
BccI CCATC 4 cut(s) 373, 770, 1180, 1184
BceAI ACGGC 1 cut(s) 254
BcgI CGANNNNNNTGC 4 cut(s) 1445, 1451, 1479, 1485
BciT130I CCWGG 2 cut(s) 235, 1262
BclI TGATCA 1 cut(s) 396
BcoDI GTCTC 2 cut(s) 410, 813
BfaI CTAG 1 cut(s) 755
BfmI CTRYAG 5 cut(s) 447, 509, 1095, 1197, 1470
BfuAI ACCTGC 1 cut(s) 1403
BisI GCNGC 8 cut(s) 183, 230, 353, 447, 456, 509, 1203, 1290
BlsI GCNGC 8 cut(s) 184, 231, 354, 448, 457, 510, 1204, 1291
BmcAI AGTACT 1 cut(s) 704
Bme1390I CCNGG 2 cut(s) 235, 1262
BmgBI CACGTC 1 cut(s) 947
BmiI GGNNCC 2 cut(s) 1206, 1404
BmrFI CCNGG 2 cut(s) 235, 1262
BmrI ACTGGG 1 cut(s) 1504
BmsI GCATC 4 cut(s) 159, 661, 1408, 1453
BmtI GCTAGC 1 cut(s) 758
BmuI ACTGGG 1 cut(s) 1504
BpmI CTGGAG 2 cut(s) 1191, 1389
Bpu10I CCTNAGC 1 cut(s) 348
Bpu14I TTCGAA 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 277, 747
BsaAI YACGTR 1 cut(s) 101
BsaBI GATNNNNATC 1 cut(s) 1269
BsaJI CCNNGG 3 cut(s) 233, 690, 1118
BsaXI ACNNNNNCTCC 2 cut(s) 686, 716
Bse118I RCCGGY 1 cut(s) 26
Bse1I ACTGG 5 cut(s) 244, 396, 966, 1406, 1499
Bse3DI GCAATG 3 cut(s) 129, 730, 793
Bse8I GATNNNNATC 1 cut(s) 1269
BseBI CCWGG 2 cut(s) 235, 1262
BseDI CCNNGG 3 cut(s) 233, 690, 1118
BseGI GGATG 7 cut(s) 676, 703, 1128, 1143, 1195, 1270, 1489
BseJI GATNNNNATC 1 cut(s) 1269
BseMI GCAATG 3 cut(s) 129, 730, 793
BseMII CTCAG 2 cut(s) 557, 732
BseNI ACTGG 5 cut(s) 244, 396, 966, 1406, 1499
BseRI GAGGAG 1 cut(s) 882
BseXI GCAGC 6 cut(s) 241, 364, 433, 442, 495, 1276
BsgI GTGCAG 2 cut(s) 326, 557
Bsh1236I CGCG 2 cut(s) 287, 928
BshFI GGCC 3 cut(s) 26, 243, 1101
BsiHKAI GWGCWC 2 cut(s) 282, 934
BsiSI CCGG 3 cut(s) 27, 212, 244
BsmAI GTCTC 2 cut(s) 410, 813
BsnI GGCC 3 cut(s) 26, 243, 1101
Bsp119I TTCGAA 1 cut(s) 114
Bsp1286I GDGCHC 2 cut(s) 282, 934
Bsp143I GATC 3 cut(s) 396, 973, 1357
BspACI CCGC 3 cut(s) 183, 805, 1202
BspANI GGCC 3 cut(s) 26, 243, 1101
BspCNI CTCAG 2 cut(s) 556, 733
BspFNI CGCG 2 cut(s) 287, 928
BspLI GGNNCC 2 cut(s) 1206, 1404
BspMAI CTGCAG 3 cut(s) 451, 513, 1474
BspMI ACCTGC 1 cut(s) 1403
BspOI GCTAGC 1 cut(s) 758
BspPI GGATC 2 cut(s) 968, 1352
BspT104I TTCGAA 1 cut(s) 114
BsrDI GCAATG 3 cut(s) 129, 730, 793
BsrFI RCCGGY 1 cut(s) 26
BsrI ACTGG 5 cut(s) 244, 396, 966, 1406, 1499
BssAI RCCGGY 1 cut(s) 26
BssECI CCNNGG 3 cut(s) 233, 690, 1118
BssMI GATC 3 cut(s) 396, 973, 1357
BssT1I CCWWGG 2 cut(s) 690, 1118
Bst2UI CCWGG 2 cut(s) 235, 1262
Bst4CI ACNGT 4 cut(s) 578, 622, 647, 1198
Bst6I CTCTTC 2 cut(s) 6, 1392
BstAPI GCANNNNNTGC 2 cut(s) 455, 1418
BstBAI YACGTR 1 cut(s) 101
BstBI TTCGAA 1 cut(s) 114
BstC8I GCNNGC 4 cut(s) 28, 180, 756, 930
BstDEI CTNAG 4 cut(s) 348, 504, 543, 741
BstEII GGTNACC 1 cut(s) 4
BstENI CCTNNNNNAGG 1 cut(s) 1148
BstF5I GGATG 7 cut(s) 676, 703, 1128, 1143, 1195, 1270, 1489
BstFNI CGCG 2 cut(s) 287, 928
BstHHI GCGC 3 cut(s) 289, 676, 928
BstKTI GATC 3 cut(s) 399, 976, 1360
BstMAI GTCTC 2 cut(s) 410, 813
BstMBI GATC 3 cut(s) 396, 973, 1357
BstNI CCWGG 2 cut(s) 235, 1262
BstNSI RCATGY 2 cut(s) 826, 938
BstPI GGTNACC 1 cut(s) 4
BstSCI CCNGG 2 cut(s) 233, 1260
BstSFI CTRYAG 5 cut(s) 447, 509, 1095, 1197, 1470
BstSNI TACGTA 1 cut(s) 101
BstUI CGCG 2 cut(s) 287, 928
BstV1I GCAGC 6 cut(s) 241, 364, 433, 442, 495, 1276
BsuRI GGCC 3 cut(s) 26, 243, 1101
BtgZI GCGATG 1 cut(s) 507
BtrI CACGTC 1 cut(s) 947
BtsCI GGATG 7 cut(s) 676, 703, 1128, 1143, 1195, 1270, 1489
BtsI GCAGTG 1 cut(s) 709
BtsIMutI CAGTG 3 cut(s) 448, 709, 973
BveI ACCTGC 1 cut(s) 1403
Cac8I GCNNGC 4 cut(s) 28, 180, 756, 930
CaiI CAGNNNCTG 2 cut(s) 455, 1088
CfoI GCGC 3 cut(s) 289, 676, 928
Cfr10I RCCGGY 1 cut(s) 26
CseI GACGC 1 cut(s) 293
Csp6I GTAC 4 cut(s) 565, 703, 842, 1194
CviAII CATG 6 cut(s) 145, 823, 935, 992, 1012, 1342
CviQI GTAC 4 cut(s) 565, 703, 842, 1194
DdeI CTNAG 4 cut(s) 348, 504, 543, 741
DpnI GATC 3 cut(s) 398, 975, 1359
DpnII GATC 3 cut(s) 396, 973, 1357
DrdI GACNNNNNNGTC 1 cut(s) 1451
DseDI GACNNNNNNGTC 1 cut(s) 1451
EaeI YGGCCR 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 6, 1392
EarI CTCTTC 2 cut(s) 6, 1392
Eco105I TACGTA 1 cut(s) 101
Eco130I CCWWGG 2 cut(s) 690, 1118
Eco57I CTGAAG 2 cut(s) 869, 1260
Eco91I GGTNACC 1 cut(s) 4
EcoNI CCTNNNNNAGG 1 cut(s) 1148
EcoO65I GGTNACC 1 cut(s) 4
EcoRII CCWGG 2 cut(s) 233, 1260
EcoT14I CCWWGG 2 cut(s) 690, 1118
ErhI CCWWGG 2 cut(s) 690, 1118
FaeI CATG 6 cut(s) 148, 826, 938, 995, 1015, 1345
FatI CATG 6 cut(s) 144, 822, 934, 991, 1011, 1341
FauI CCCGC 1 cut(s) 798
FbaI TGATCA 1 cut(s) 396
Fnu4HI GCNGC 8 cut(s) 183, 230, 353, 447, 456, 509, 1203, 1290
FokI GGATG 7 cut(s) 683, 710, 1135, 1150, 1202, 1277, 1496
Fsp4HI GCNGC 8 cut(s) 183, 230, 353, 447, 456, 509, 1203, 1290
FspBI CTAG 1 cut(s) 755
GlaI GCGC 3 cut(s) 288, 675, 927
GluI GCNGC 8 cut(s) 183, 230, 353, 447, 456, 509, 1203, 1290
GsuI CTGGAG 2 cut(s) 1191, 1389
HaeIII GGCC 3 cut(s) 26, 243, 1101
HapII CCGG 3 cut(s) 27, 212, 244
HgaI GACGC 1 cut(s) 293
HhaI GCGC 3 cut(s) 289, 676, 928
Hin1II CATG 6 cut(s) 148, 826, 938, 995, 1015, 1345
Hin6I GCGC 3 cut(s) 287, 674, 926
HinP1I GCGC 3 cut(s) 287, 674, 926
HindIII AAGCTT 2 cut(s) 915, 1278
HinfI GANTC 8 cut(s) 50, 59, 264, 514, 962, 1085, 1316, 1327
HpaII CCGG 3 cut(s) 27, 212, 244
HphI GGTGA 7 cut(s) 230, 319, 406, 889, 971, 983, 1427
Hpy188I TCNGA 5 cut(s) 401, 546, 1084, 1090, 1219
Hpy188III TCNNGA 6 cut(s) 172, 533, 626, 764, 896, 1208
Hpy99I CGWCG 4 cut(s) 158, 275, 287, 948
HpyAV CCTTC 7 cut(s) 75, 209, 406, 482, 1052, 1246, 1361
HpyCH4III ACNGT 4 cut(s) 578, 622, 647, 1198
HpyCH4IV ACGT 4 cut(s) 100, 156, 946, 1354
HpyCH4V TGCA 9 cut(s) 343, 370, 449, 511, 574, 723, 791, 1412, 1472
HpyF3I CTNAG 4 cut(s) 348, 504, 543, 741
HpySE526I ACGT 4 cut(s) 100, 156, 946, 1354
Hsp92II CATG 6 cut(s) 148, 826, 938, 995, 1015, 1345
HspAI GCGC 3 cut(s) 287, 674, 926
KroI GCCGGC 1 cut(s) 26
KroNI GCCGGC 1 cut(s) 28
Ksp22I TGATCA 1 cut(s) 396
Kzo9I GATC 3 cut(s) 396, 973, 1357
LmnI GCTCC 3 cut(s) 730, 1210, 1408
Lsp1109I GCAGC 6 cut(s) 241, 364, 433, 442, 495, 1276
LweI GCATC 4 cut(s) 159, 661, 1408, 1453
MaeI CTAG 1 cut(s) 755
MaeII ACGT 4 cut(s) 100, 156, 946, 1354
MaeIII GTNAC 3 cut(s) 4, 89, 1067
MalI GATC 3 cut(s) 398, 975, 1359
MboI GATC 3 cut(s) 396, 973, 1357
MhlI GDGCHC 2 cut(s) 282, 934
MluCI AATT 7 cut(s) 123, 371, 602, 812, 867, 899, 1429
MlyI GAGTC 2 cut(s) 508, 1336
MroNI GCCGGC 1 cut(s) 26
MroXI GAANNNNTTC 2 cut(s) 437, 1256
MseI TTAA 3 cut(s) 815, 857, 870
MslI CAYNNNNRTG 1 cut(s) 996
MspA1I CMGCKG 1 cut(s) 847
MspI CCGG 3 cut(s) 27, 212, 244
MspR9I CCNGG 2 cut(s) 235, 1262
MvaI CCWGG 2 cut(s) 235, 1262
MvnI CGCG 2 cut(s) 287, 928
NaeI GCCGGC 1 cut(s) 28
NdeII GATC 3 cut(s) 396, 973, 1357
NgoMIV GCCGGC 1 cut(s) 26
NheI GCTAGC 1 cut(s) 754
NlaIII CATG 6 cut(s) 148, 826, 938, 995, 1015, 1345
NlaIV GGNNCC 2 cut(s) 1206, 1404
NmeAIII GCCGAG 1 cut(s) 976
NmuCI GTSAC 1 cut(s) 89
NspI RCATGY 2 cut(s) 826, 938
NspV TTCGAA 1 cut(s) 114
PaqCI CACCTGC 1 cut(s) 1403
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 28
PdmI GAANNNNTTC 2 cut(s) 437, 1256
PfeI GAWTC 6 cut(s) 50, 59, 264, 962, 1085, 1316
PfoI TCCNGGA 1 cut(s) 1260
PkrI GCNGC 8 cut(s) 184, 231, 354, 448, 457, 510, 1204, 1291
PleI GAGTC 2 cut(s) 508, 1335
PpsI GAGTC 2 cut(s) 508, 1335
Ppu21I YACGTR 1 cut(s) 101
PshBI ATTAAT 1 cut(s) 870
Psp6I CCWGG 2 cut(s) 233, 1260
PspEI GGTNACC 1 cut(s) 4
PspGI CCWGG 2 cut(s) 233, 1260
PspN4I GGNNCC 2 cut(s) 1206, 1404
PstI CTGCAG 3 cut(s) 451, 513, 1474
PstNI CAGNNNCTG 2 cut(s) 455, 1088
PvuII CAGCTG 1 cut(s) 847
RsaI GTAC 4 cut(s) 566, 704, 843, 1195
RsaNI GTAC 4 cut(s) 565, 703, 842, 1194
RseI CAYNNNNRTG 1 cut(s) 996
SaqAI TTAA 3 cut(s) 815, 857, 870
SatI GCNGC 8 cut(s) 183, 230, 353, 447, 456, 509, 1203, 1290
Sau3AI GATC 3 cut(s) 396, 973, 1357
ScaI AGTACT 1 cut(s) 704
SchI GAGTC 2 cut(s) 508, 1336
ScrFI CCNGG 2 cut(s) 235, 1262
SduI GDGCHC 2 cut(s) 282, 934
SfaNI GCATC 4 cut(s) 159, 661, 1408, 1453
SfcI CTRYAG 5 cut(s) 447, 509, 1095, 1197, 1470
SfuI TTCGAA 1 cut(s) 114
SmiMI CAYNNNNRTG 1 cut(s) 996
SmlI CTYRAG 2 cut(s) 292, 762
SmoI CTYRAG 2 cut(s) 292, 762
SnaBI TACGTA 1 cut(s) 101
Sse9I AATT 7 cut(s) 123, 371, 602, 812, 867, 899, 1429
SsiI CCGC 3 cut(s) 183, 805, 1202
SspMI CTAG 1 cut(s) 755
StyD4I CCNGG 2 cut(s) 233, 1260
StyI CCWWGG 2 cut(s) 690, 1118
TaaI ACNGT 4 cut(s) 578, 622, 647, 1198
TaiI ACGT 4 cut(s) 103, 159, 949, 1357
TaqI TCGA 4 cut(s) 114, 153, 282, 1455
TasI AATT 7 cut(s) 123, 371, 602, 812, 867, 899, 1429
TatI WGTACW 1 cut(s) 702
TauI GCSGC 2 cut(s) 185, 1205
TfiI GAWTC 6 cut(s) 50, 59, 264, 962, 1085, 1316
Tru1I TTAA 3 cut(s) 815, 857, 870
Tru9I TTAA 3 cut(s) 815, 857, 870
TscAI CASTG 3 cut(s) 448, 716, 973
TseFI GTSAC 1 cut(s) 89
TseI GCWGC 6 cut(s) 229, 352, 446, 455, 508, 1289
Tsp45I GTSAC 1 cut(s) 89
TspDTI ATGAA 8 cut(s) 25, 615, 1071, 1154, 1313, 1358, 1380, 1389
TspGWI ACGGA 1 cut(s) 1440
TspRI CASTG 3 cut(s) 448, 716, 973
VspI ATTAAT 1 cut(s) 870
XagI CCTNNNNNAGG 1 cut(s) 1148
XapI RAATTY 1 cut(s) 899
XceI RCATGY 2 cut(s) 826, 938
XmnI GAANNNNTTC 2 cut(s) 437, 1256
XspI CTAG 1 cut(s) 755
ZrmI AGTACT 1 cut(s) 704
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.