RchiOBHm_Chr7g0184551

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0001364
Physical Location & Seq
Reverse (-)
5052337 .. 5056021
3685 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ16467

Sequence Viewer

Length: 1116 bp
ATGAGCATCGAGGTTTTCAACAGGTTTGTGAGGCTTGCGGCCAGAGCGTTCTACGATGACGTCACGCCGGAAAGTGATAATAAGCAGCCCAAGACTGGCCGGAGCGACAATAGGGGAATCGCCGTCGTGGTGATCGACGCGCTATCAAGGCGACAATGGGTGAGAGAAGAAGATCTGGCGAAGGACCTGAAACTGCACCCCAAGCAACTTCGTCGTGTTTTGCAAATGTTGATGGAGGAAAAGTTGGTGATCAGAGAACATAGGAAAGAGACAGCTAAGGGTGTGAAGATTTTCAGTGCTGCGGTTGCTGCCACAGCCAATGGGCAACAACACAGAAAAGAAGGCGAAGAGAAAATTAAGCTGCAGACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCTTTTGAAGAAAGAACTGAAAGATGAACTGGAGGGGAAGCACGCAGTGGAGGAATATGTATGCCATAACTGCAGGAAAAGATACAACGCATTGGATGCACTGCAACTGGTTTCTATGGAGGATGAATACTTCCACTGCGAAAGTTGCAATGGGATACTTGTGGCTGAGAGTGACAAGCTAGATTCTCAAACCACAGCAGGCCGAGACTGCAAAGCAATGCTCCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGAGTTAAAGATTTACCATTTCCTGAATTTGGAAGTCTCCAAGCTTGGGAAGCCAGAGCAAGTGCTCCACGACTTGCAGAGAATAGTGAATCTAGTGCCATTGATCCCTCCATATCTTCTCATGGGCTGGGGTATGGTGGGACGCCCATGCCTTACGTTGGAGATACAAAGGTTGAAGTTGTGTTTTCTAAGAATGAGGACAAGGGTAAGGATATAAAATCTCAAGCTGATAATACAGACCTAAAAGTTATACCACCTTGGATGATCAAGCAAGAGATGACTCTTACAAGGGAACAGTGTGGAGAGGTCAGGCAAATGGAATCCTCACAAATGCACATCTCTAGTCATCAGGTTCGTCGGGTTGGCATGAAGTCAGAACGGGATGAGGATGAAGGAGATGATAATATTGACTGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

371

Amino Acids

42.5

Weight (kDa)

5.86

Isoelectric Point (pI)

47.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 41 - 95 4.6e-08 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 63
AccB7I CCANNNNNTGG 1 cut(s) 658
AccII CGCG 1 cut(s) 140
AciI CCGC 2 cut(s) 38, 302
AclWI GGATC 1 cut(s) 794
AcoI YGGCCR 2 cut(s) 39, 97
AcsI RAATTY 1 cut(s) 722
AcuI CTGAAG 1 cut(s) 692
AcyI GRCGYC 2 cut(s) 60, 839
AdeI CACNNNGTG 1 cut(s) 475
AfaI GTAC 2 cut(s) 419, 666
AfiI CCNNNNNNNGG 5 cut(s) 95, 658, 725, 742, 854
AgsI TTSAA 3 cut(s) 19, 436, 872
AluBI AGCT 6 cut(s) 275, 361, 607, 670, 740, 923
AluI AGCT 6 cut(s) 275, 361, 607, 670, 740, 923
Alw21I GWGCWC 1 cut(s) 763
Alw26I GTCTC 3 cut(s) 263, 627, 737
AlwI GGATC 1 cut(s) 794
AoxI GGCC 3 cut(s) 39, 97, 628
ApeKI GCWGC 4 cut(s) 85, 299, 308, 361
ApoI RAATTY 1 cut(s) 722
AseI ATTAAT 1 cut(s) 693
Asp700I GAANNNNTTC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 142
AspS9I GGNCC 1 cut(s) 184
AsuHPI GGTGA 3 cut(s) 142, 172, 259
AvaII GGWCC 1 cut(s) 184
BaeI ACNNNNGTAYC 2 cut(s) 852, 885
Bbv12I GWGCWC 1 cut(s) 763
BbvI GCAGC 4 cut(s) 97, 286, 295, 348
BccI CCATC 1 cut(s) 226
BceAI ACGGC 1 cut(s) 107
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciVI GTATCC 1 cut(s) 576
BclI TGATCA 2 cut(s) 249, 960
BcoDI GTCTC 3 cut(s) 263, 627, 737
BfaI CTAG 3 cut(s) 608, 789, 1038
BfmI CTRYAG 2 cut(s) 362, 499
BfuI GTATCC 1 cut(s) 576
BglII AGATCT 1 cut(s) 172
BisI GCNGC 5 cut(s) 39, 86, 300, 309, 362
BlsI GCNGC 5 cut(s) 40, 87, 301, 310, 363
Bme18I GGWCC 1 cut(s) 184
BmgT120I GGNCC 1 cut(s) 184
BmsI GCATC 2 cut(s) 15, 514
BpmI CTGGAG 1 cut(s) 479
Bpu10I CCTNAGC 1 cut(s) 276
BpuEI CTTGAG 1 cut(s) 903
BsaHI GRCGYC 2 cut(s) 60, 839
BsaJI CCNNGG 1 cut(s) 953
BsaXI ACNNNNNCTCC 2 cut(s) 227, 257
Bsc4I CCNNNNNNNGG 5 cut(s) 95, 658, 725, 742, 854
Bse1I ACTGG 4 cut(s) 100, 462, 540, 1112
Bse3DI GCAATG 2 cut(s) 583, 651
BseDI CCNNGG 1 cut(s) 953
BseGI GGATG 5 cut(s) 529, 556, 963, 1084, 1090
BseLI CCNNNNNNNGG 5 cut(s) 95, 658, 725, 742, 854
BseMI GCAATG 2 cut(s) 583, 651
BseMII CTCAG 2 cut(s) 410, 585
BseNI ACTGG 4 cut(s) 100, 462, 540, 1112
BseXI GCAGC 4 cut(s) 97, 286, 295, 348
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 2 cut(s) 179, 410
Bsh1236I CGCG 1 cut(s) 140
BshFI GGCC 3 cut(s) 41, 99, 630
BsiHKAI GWGCWC 1 cut(s) 763
BsiSI CCGG 2 cut(s) 68, 100
BslFI GGGAC 1 cut(s) 850
BslI CCNNNNNNNGG 5 cut(s) 95, 658, 725, 742, 854
BsmAI GTCTC 3 cut(s) 263, 627, 737
BsmFI GGGAC 1 cut(s) 850
BsnI GGCC 3 cut(s) 41, 99, 630
Bsp1286I GDGCHC 1 cut(s) 763
Bsp143I GATC 5 cut(s) 132, 172, 249, 799, 960
BspACI CCGC 2 cut(s) 38, 302
BspANI GGCC 3 cut(s) 41, 99, 630
BspCNI CTCAG 2 cut(s) 409, 586
BspFNI CGCG 1 cut(s) 140
BspMAI CTGCAG 2 cut(s) 366, 503
BspPI GGATC 1 cut(s) 794
BsrDI GCAATG 2 cut(s) 583, 651
BsrI ACTGG 4 cut(s) 100, 462, 540, 1112
BssECI CCNNGG 1 cut(s) 953
BssMI GATC 5 cut(s) 132, 172, 249, 799, 960
BssNI GRCGYC 2 cut(s) 60, 839
BssT1I CCWWGG 1 cut(s) 953
Bst4CI ACNGT 1 cut(s) 993
Bst6I CTCTTC 1 cut(s) 342
BstACI GRCGYC 2 cut(s) 60, 839
BstAPI GCANNNNNTGC 1 cut(s) 524
BstC8I GCNNGC 3 cut(s) 36, 471, 628
BstDEI CTNAG 4 cut(s) 276, 396, 594, 885
BstF5I GGATG 5 cut(s) 529, 556, 963, 1084, 1090
BstFNI CGCG 1 cut(s) 140
BstHHI GCGC 1 cut(s) 142
BstKTI GATC 5 cut(s) 135, 175, 252, 802, 963
BstMAI GTCTC 3 cut(s) 263, 627, 737
BstMBI GATC 5 cut(s) 132, 172, 249, 799, 960
BstSFI CTRYAG 2 cut(s) 362, 499
BstUI CGCG 1 cut(s) 140
BstV1I GCAGC 4 cut(s) 97, 286, 295, 348
BstX2I RGATCY 1 cut(s) 172
BstYI RGATCY 1 cut(s) 172
BsuI GTATCC 1 cut(s) 576
BsuRI GGCC 3 cut(s) 41, 99, 630
BtsCI GGATG 5 cut(s) 529, 556, 963, 1084, 1090
BtsI GCAGTG 3 cut(s) 480, 527, 562
BtsIMutI CAGTG 5 cut(s) 301, 480, 527, 562, 998
Cac8I GCNNGC 3 cut(s) 36, 471, 628
CfoI GCGC 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 184
CseI GACGC 2 cut(s) 146, 847
Csp6I GTAC 2 cut(s) 418, 665
CviAII CATG 3 cut(s) 818, 844, 1063
CviQI GTAC 2 cut(s) 418, 665
DdeI CTNAG 4 cut(s) 276, 396, 594, 885
DpnI GATC 5 cut(s) 134, 174, 251, 801, 962
DpnII GATC 5 cut(s) 132, 172, 249, 799, 960
DraIII CACNNNGTG 1 cut(s) 475
EaeI YGGCCR 2 cut(s) 39, 97
Eam1104I CTCTTC 1 cut(s) 342
EarI CTCTTC 1 cut(s) 342
Eco130I CCWWGG 1 cut(s) 953
Eco47I GGWCC 1 cut(s) 184
Eco57I CTGAAG 1 cut(s) 692
EcoO109I RGGNCCY 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 953
ErhI CCWWGG 1 cut(s) 953
FaeI CATG 3 cut(s) 821, 847, 1066
FaqI GGGAC 1 cut(s) 850
FatI CATG 3 cut(s) 817, 843, 1062
FbaI TGATCA 2 cut(s) 249, 960
Fnu4HI GCNGC 5 cut(s) 39, 86, 300, 309, 362
FokI GGATG 5 cut(s) 536, 563, 970, 1091, 1097
Fsp4HI GCNGC 5 cut(s) 39, 86, 300, 309, 362
FspBI CTAG 3 cut(s) 608, 789, 1038
GlaI GCGC 1 cut(s) 141
GluI GCNGC 5 cut(s) 39, 86, 300, 309, 362
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 1 cut(s) 479
HaeIII GGCC 3 cut(s) 41, 99, 630
HapII CCGG 2 cut(s) 68, 100
HgaI GACGC 2 cut(s) 146, 847
HhaI GCGC 1 cut(s) 142
Hin1I GRCGYC 2 cut(s) 60, 839
Hin1II CATG 3 cut(s) 821, 847, 1066
Hin6I GCGC 1 cut(s) 140
HinP1I GCGC 1 cut(s) 140
HindIII AAGCTT 1 cut(s) 738
HinfI GANTC 6 cut(s) 117, 367, 611, 785, 976, 1016
HpaII CCGG 2 cut(s) 68, 100
HphI GGTGA 3 cut(s) 142, 172, 259
Hpy188I TCNGA 3 cut(s) 254, 399, 1072
Hpy188III TCNNGA 2 cut(s) 386, 719
Hpy99I CGWCG 4 cut(s) 128, 140, 216, 1056
HpyAV CCTTC 3 cut(s) 175, 335, 1082
HpyCH4III ACNGT 1 cut(s) 993
HpyCH4IV ACGT 2 cut(s) 60, 852
HpyF3I CTNAG 4 cut(s) 276, 396, 594, 885
HpySE526I ACGT 2 cut(s) 60, 852
Hsp92I GRCGYC 2 cut(s) 60, 839
Hsp92II CATG 3 cut(s) 821, 847, 1066
HspAI GCGC 1 cut(s) 140
Ksp22I TGATCA 2 cut(s) 249, 960
Kzo9I GATC 5 cut(s) 132, 172, 249, 799, 960
LmnI GCTCC 3 cut(s) 102, 654, 766
Lsp1109I GCAGC 4 cut(s) 97, 286, 295, 348
LweI GCATC 2 cut(s) 15, 514
MaeI CTAG 3 cut(s) 608, 789, 1038
MaeII ACGT 2 cut(s) 60, 852
MaeIII GTNAC 2 cut(s) 61, 599
MalI GATC 5 cut(s) 134, 174, 251, 801, 962
MboI GATC 5 cut(s) 132, 172, 249, 799, 960
MboII GAAGA 6 cut(s) 179, 182, 298, 359, 448, 804
MflI RGATCY 1 cut(s) 172
MhlI GDGCHC 1 cut(s) 763
MluCI AATT 3 cut(s) 354, 690, 722
MlyI GAGTC 2 cut(s) 361, 970
MmeI TCCRAC 1 cut(s) 835
MroXI GAANNNNTTC 1 cut(s) 290
MseI TTAA 4 cut(s) 357, 680, 693, 702
MspA1I CMGCKG 1 cut(s) 670
MspI CCGG 2 cut(s) 68, 100
MvnI CGCG 1 cut(s) 140
NdeII GATC 5 cut(s) 132, 172, 249, 799, 960
NlaIII CATG 3 cut(s) 821, 847, 1066
NmeAIII GCCGAG 1 cut(s) 656
NmuCI GTSAC 2 cut(s) 61, 599
PcsI WCGNNNNNNNCGW 1 cut(s) 132
PdmI GAANNNNTTC 1 cut(s) 290
PfeI GAWTC 4 cut(s) 117, 611, 785, 1016
PflMI CCANNNNNTGG 1 cut(s) 658
PkrI GCNGC 5 cut(s) 40, 87, 301, 310, 363
PleI GAGTC 2 cut(s) 361, 970
PpsI GAGTC 2 cut(s) 361, 970
PpuMI RGGWCCY 1 cut(s) 184
PshBI ATTAAT 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 184
PspFI CCCAGC 1 cut(s) 823
PspPI GGNCC 1 cut(s) 184
PspPPI RGGWCCY 1 cut(s) 184
PstI CTGCAG 2 cut(s) 366, 503
PsuI RGATCY 1 cut(s) 172
PvuII CAGCTG 1 cut(s) 670
RsaI GTAC 2 cut(s) 419, 666
RsaNI GTAC 2 cut(s) 418, 665
SaqAI TTAA 4 cut(s) 357, 680, 693, 702
SatI GCNGC 5 cut(s) 39, 86, 300, 309, 362
Sau3AI GATC 5 cut(s) 132, 172, 249, 799, 960
Sau96I GGNCC 1 cut(s) 184
SchI GAGTC 2 cut(s) 361, 970
SduI GDGCHC 1 cut(s) 763
SfaNI GCATC 2 cut(s) 15, 514
SfcI CTRYAG 2 cut(s) 362, 499
SinI GGWCC 1 cut(s) 184
SmlI CTYRAG 1 cut(s) 918
SmoI CTYRAG 1 cut(s) 918
Sse9I AATT 3 cut(s) 354, 690, 722
SsiI CCGC 2 cut(s) 38, 302
SspI AATATT 1 cut(s) 1102
SspMI CTAG 3 cut(s) 608, 789, 1038
StyI CCWWGG 1 cut(s) 953
TaaI ACNGT 1 cut(s) 993
TaiI ACGT 2 cut(s) 63, 855
TaqI TCGA 2 cut(s) 9, 135
TasI AATT 3 cut(s) 354, 690, 722
TauI GCSGC 1 cut(s) 41
TfiI GAWTC 4 cut(s) 117, 611, 785, 1016
Tru1I TTAA 4 cut(s) 357, 680, 693, 702
Tru9I TTAA 4 cut(s) 357, 680, 693, 702
TscAI CASTG 5 cut(s) 301, 480, 534, 569, 998
TseFI GTSAC 2 cut(s) 61, 599
TseI GCWGC 4 cut(s) 85, 299, 308, 361
Tsp45I GTSAC 2 cut(s) 61, 599
TspDTI ATGAA 4 cut(s) 468, 567, 1079, 1101
TspRI CASTG 5 cut(s) 301, 480, 534, 569, 998
Van91I CCANNNNNTGG 1 cut(s) 658
VpaK11BI GGWCC 1 cut(s) 184
VspI ATTAAT 1 cut(s) 693
XapI RAATTY 1 cut(s) 722
XmnI GAANNNNTTC 1 cut(s) 290
XspI CTAG 3 cut(s) 608, 789, 1038
ZraI GACGTC 1 cut(s) 61
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.