RLG00000004968

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0001364
Physical Location & Seq
Forward (+)
64064896 .. 64070056
5161 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004968

Sequence Viewer

Length: 1071 bp
ATGAGTATCGAGCCTTTCAATAGGTTGGTGAAGCTCGCGGCGAGGGCGTTCTACGATGACATAACCACGAAAGGGGATAATCAGCCCAAGACTGGCCGCAGCGACAATCGGGGAATTGCCGTGGTTGTGCTTGACGCGCTCTCAAGGAAGAATTTGCGAGCTGTTTGTTCCACAGCTAAGGGTGCAAAGATATTTAGTGCTGCAGTTGCGGCAACAGCTGACGGGCAACAACCTACAAAAGAAGGGGAAGAGAAAATTAAGTTGCACACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCGTATGAGGAAAAAACTGAAAGATGAATTGGAGGACAAGAACACAGTTCAGGAATATGTATGCCCTAACTGTGGGAAAAGATACAATGCTTTGGATGCACTGCAACTCATTTCTTTGGAGGATGAGTACTTCCACTGCGAGAGTTGCAATGGGGAGCTTGTGGCTGAGAGTGACAAGCTAGCTTCTCAAGATGTAGGAGATGGAGATAGCAATGCAAGGAGGCAAAGGCGAGAAAAACTGAAAGACATGCTTCAAAATATGGAGGTTGAAGTTGATTTTGGGAATGAAGGCAAGGGTAACGATGTCAAATCAGAAGCTGATATTACAGGCCAGAAAATTTTGCCACCTTGGATGATAAAGCAAGGGATGAACCTTACAAAGGAACAGCGTGGAGAGGTAAAGCAGGAGGCAAAGATGGATGGCACTTTAGCAGCTCCAGAGTATTCAGATGACAAAAAGTCAAATGCTGAAGATAATGAGAAGGCTTTCCAGGATGAGTATTTCAAAGCCTATTACGCTGCTTTAATAAAAAGACAAGAAGAAGAAGCAGCAAAGAGTCGTCAGGTTGGCATGAAGTCAAAACGTGATCCGAATGAAGGAGATGAAGGAGAAGATAATGTTGACTGGCAAGAGGCTCCAGTTGCAGGTGATGCCAATGGAAATTACAAGGTCAATGACTTAAACGTCGAAGCCGATGCTACTGCAGAGGATGAAGATGACATAGACTGGGAGGAAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

357

Amino Acids

40.03

Weight (kDa)

4.81

Isoelectric Point (pI)

39.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 965
AasI GACNNNNNNGTC 1 cut(s) 1013
Acc36I ACCTGC 1 cut(s) 965
AccII CGCG 2 cut(s) 38, 137
AciI CCGC 3 cut(s) 38, 97, 209
AclWI GGATC 1 cut(s) 911
AcoI YGGCCR 1 cut(s) 94
AcsI RAATTY 2 cut(s) 151, 666
AcuI CTGAAG 1 cut(s) 819
AfaI GTAC 2 cut(s) 320, 458
AfiI CCNNNNNNNGG 6 cut(s) 72, 92, 401, 709, 926, 974
AgsI TTSAA 4 cut(s) 19, 584, 599, 835
AhdI GACNNNNNGTC 1 cut(s) 787
AjnI CCWGG 1 cut(s) 819
AjuI GAANNNNNNNTTGG 4 cut(s) 341, 373, 591, 623
AluBI AGCT 9 cut(s) 34, 161, 176, 218, 487, 508, 512, 647, 764
AluI AGCT 9 cut(s) 34, 161, 176, 218, 487, 508, 512, 647, 764
AlwI GGATC 1 cut(s) 911
AlwNI CAGNNNCTG 1 cut(s) 647
AoxI GGCC 2 cut(s) 94, 658
ApeKI GCWGC 5 cut(s) 99, 200, 761, 848, 878
ApoI RAATTY 2 cut(s) 151, 666
Asp700I GAANNNNTTC 1 cut(s) 815
AspLEI GCGC 1 cut(s) 139
AsuHPI GGTGA 2 cut(s) 40, 989
AsuNHI GCTAGC 1 cut(s) 508
BbvI GCAGC 5 cut(s) 111, 187, 773, 835, 890
BccI CCATC 3 cut(s) 524, 739, 743
BceAI ACGGC 1 cut(s) 104
BcgI CGANNNNNNTGC 4 cut(s) 1007, 1013, 1041, 1047
BciT130I CCWGG 1 cut(s) 821
BfaI CTAG 1 cut(s) 509
BfmI CTRYAG 2 cut(s) 201, 1032
BfuAI ACCTGC 1 cut(s) 965
BisI GCNGC 8 cut(s) 39, 97, 100, 201, 210, 762, 849, 879
BlsI GCNGC 8 cut(s) 40, 98, 101, 202, 211, 763, 850, 880
BmcAI AGTACT 1 cut(s) 458
Bme1390I CCNGG 1 cut(s) 821
BmeRI GACNNNNNGTC 1 cut(s) 787
BmiI GGNNCC 1 cut(s) 966
BmrFI CCNGG 1 cut(s) 821
BmrI ACTGGG 1 cut(s) 1066
BmsI GCATC 3 cut(s) 415, 970, 1015
BmtI GCTAGC 1 cut(s) 512
BmuI ACTGGG 1 cut(s) 1066
BpmI CTGGAG 2 cut(s) 750, 951
Bpu10I CCTNAGC 1 cut(s) 177
BpuEI CTTGAG 2 cut(s) 127, 501
BsaJI CCNNGG 2 cut(s) 120, 677
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 6 cut(s) 72, 92, 401, 709, 926, 974
Bse1I ACTGG 4 cut(s) 97, 959, 968, 1061
Bse3DI GCAATG 2 cut(s) 484, 547
BseBI CCWGG 1 cut(s) 821
BseDI CCNNGG 2 cut(s) 120, 677
BseGI GGATG 7 cut(s) 430, 457, 687, 702, 754, 829, 1045
BseLI CCNNNNNNNGG 6 cut(s) 72, 92, 401, 709, 926, 974
BseMI GCAATG 2 cut(s) 484, 547
BseMII CTCAG 2 cut(s) 311, 486
BseNI ACTGG 4 cut(s) 97, 959, 968, 1061
BseXI GCAGC 5 cut(s) 111, 187, 773, 835, 890
BsgI GTGCAG 1 cut(s) 311
Bsh1236I CGCG 2 cut(s) 38, 137
BshFI GGCC 2 cut(s) 96, 660
BslI CCNNNNNNNGG 6 cut(s) 72, 92, 401, 709, 926, 974
BsnI GGCC 2 cut(s) 96, 660
Bsp143I GATC 1 cut(s) 916
BspACI CCGC 3 cut(s) 38, 97, 209
BspANI GGCC 2 cut(s) 96, 660
BspCNI CTCAG 2 cut(s) 310, 487
BspFNI CGCG 2 cut(s) 38, 137
BspLI GGNNCC 1 cut(s) 966
BspMAI CTGCAG 2 cut(s) 205, 1036
BspMI ACCTGC 1 cut(s) 965
BspOI GCTAGC 1 cut(s) 512
BspPI GGATC 1 cut(s) 911
BsrDI GCAATG 2 cut(s) 484, 547
BsrI ACTGG 4 cut(s) 97, 959, 968, 1061
BssECI CCNNGG 2 cut(s) 120, 677
BssMI GATC 1 cut(s) 916
BssT1I CCWWGG 1 cut(s) 677
Bst2UI CCWGG 1 cut(s) 821
Bst4CI ACNGT 3 cut(s) 332, 376, 401
Bst6I CTCTTC 1 cut(s) 243
BstAPI GCANNNNNTGC 1 cut(s) 980
BstC8I GCNNGC 3 cut(s) 36, 159, 510
BstDEI CTNAG 3 cut(s) 177, 297, 495
BstDSI CCRYGG 1 cut(s) 120
BstENI CCTNNNNNAGG 1 cut(s) 707
BstF5I GGATG 7 cut(s) 430, 457, 687, 702, 754, 829, 1045
BstFNI CGCG 2 cut(s) 38, 137
BstHHI GCGC 1 cut(s) 139
BstKTI GATC 1 cut(s) 919
BstMBI GATC 1 cut(s) 916
BstNI CCWGG 1 cut(s) 821
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 1 cut(s) 819
BstSFI CTRYAG 2 cut(s) 201, 1032
BstUI CGCG 2 cut(s) 38, 137
BstV1I GCAGC 5 cut(s) 111, 187, 773, 835, 890
BsuRI GGCC 2 cut(s) 96, 660
BtgI CCRYGG 1 cut(s) 120
BtsCI GGATG 7 cut(s) 430, 457, 687, 702, 754, 829, 1045
BtsI GCAGTG 2 cut(s) 428, 463
BtsIMutI CAGTG 2 cut(s) 428, 463
BveI ACCTGC 1 cut(s) 965
Cac8I GCNNGC 3 cut(s) 36, 159, 510
CaiI CAGNNNCTG 1 cut(s) 647
CfoI GCGC 1 cut(s) 139
CseI GACGC 1 cut(s) 143
Csp6I GTAC 2 cut(s) 319, 457
CviAII CATG 2 cut(s) 577, 901
CviQI GTAC 2 cut(s) 319, 457
DdeI CTNAG 3 cut(s) 177, 297, 495
DpnI GATC 1 cut(s) 918
DpnII GATC 1 cut(s) 916
DrdI GACNNNNNNGTC 1 cut(s) 1013
DriI GACNNNNNGTC 1 cut(s) 787
DseDI GACNNNNNNGTC 1 cut(s) 1013
EaeI YGGCCR 1 cut(s) 94
Eam1104I CTCTTC 1 cut(s) 243
Eam1105I GACNNNNNGTC 1 cut(s) 787
EarI CTCTTC 1 cut(s) 243
Eco130I CCWWGG 1 cut(s) 677
Eco57I CTGAAG 1 cut(s) 819
EcoNI CCTNNNNNAGG 1 cut(s) 707
EcoRII CCWGG 1 cut(s) 819
EcoT14I CCWWGG 1 cut(s) 677
ErhI CCWWGG 1 cut(s) 677
FaeI CATG 2 cut(s) 580, 904
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FatI CATG 2 cut(s) 576, 900
Fnu4HI GCNGC 8 cut(s) 39, 97, 100, 201, 210, 762, 849, 879
FokI GGATG 7 cut(s) 437, 464, 694, 709, 761, 836, 1052
Fsp4HI GCNGC 8 cut(s) 39, 97, 100, 201, 210, 762, 849, 879
FspBI CTAG 1 cut(s) 509
GlaI GCGC 1 cut(s) 138
GluI GCNGC 8 cut(s) 39, 97, 100, 201, 210, 762, 849, 879
GsuI CTGGAG 2 cut(s) 750, 951
HaeIII GGCC 2 cut(s) 96, 660
HgaI GACGC 1 cut(s) 143
HhaI GCGC 1 cut(s) 139
Hin1II CATG 2 cut(s) 580, 904
Hin6I GCGC 1 cut(s) 137
HinP1I GCGC 1 cut(s) 137
HincII GTYRAC 1 cut(s) 952
HindII GTYRAC 1 cut(s) 952
HinfI GANTC 1 cut(s) 886
HphI GGTGA 2 cut(s) 40, 989
Hpy166II GTNNAC 1 cut(s) 952
Hpy188I TCNGA 4 cut(s) 300, 643, 778, 921
Hpy188III TCNNGA 4 cut(s) 287, 380, 518, 767
Hpy8I GTNNAC 1 cut(s) 952
Hpy99I CGWCG 1 cut(s) 1019
HpyAV CCTTC 5 cut(s) 236, 611, 805, 920, 929
HpyCH4III ACNGT 3 cut(s) 332, 376, 401
HpyCH4IV ACGT 2 cut(s) 913, 1014
HpyF3I CTNAG 3 cut(s) 177, 297, 495
HpySE526I ACGT 2 cut(s) 913, 1014
Hsp92II CATG 2 cut(s) 580, 904
HspAI GCGC 1 cut(s) 137
Kzo9I GATC 1 cut(s) 916
LmnI GCTCC 3 cut(s) 484, 769, 970
Lsp1109I GCAGC 5 cut(s) 111, 187, 773, 835, 890
LweI GCATC 3 cut(s) 415, 970, 1015
MaeI CTAG 1 cut(s) 509
MaeII ACGT 2 cut(s) 913, 1014
MaeIII GTNAC 2 cut(s) 500, 626
MalI GATC 1 cut(s) 918
MboI GATC 1 cut(s) 916
MboII GAAGA 7 cut(s) 160, 260, 812, 881, 884, 953, 1055
MluCI AATT 6 cut(s) 114, 151, 255, 356, 666, 991
MlyI GAGTC 1 cut(s) 895
MroXI GAANNNNTTC 1 cut(s) 815
MseI TTAA 3 cut(s) 258, 854, 1010
MspA1I CMGCKG 1 cut(s) 218
MspR9I CCNGG 1 cut(s) 821
MvaI CCWGG 1 cut(s) 821
MvnI CGCG 2 cut(s) 38, 137
NdeII GATC 1 cut(s) 916
NheI GCTAGC 1 cut(s) 508
NlaIII CATG 2 cut(s) 580, 904
NlaIV GGNNCC 1 cut(s) 966
NmuCI GTSAC 1 cut(s) 500
NspI RCATGY 1 cut(s) 580
PaqCI CACCTGC 1 cut(s) 965
PcsI WCGNNNNNNNCGW 1 cut(s) 1020
PdmI GAANNNNTTC 1 cut(s) 815
PfoI TCCNGGA 1 cut(s) 819
PkrI GCNGC 8 cut(s) 40, 98, 101, 202, 211, 763, 850, 880
PleI GAGTC 1 cut(s) 894
PpsI GAGTC 1 cut(s) 894
Psp6I CCWGG 1 cut(s) 819
PspGI CCWGG 1 cut(s) 819
PspN4I GGNNCC 1 cut(s) 966
PstI CTGCAG 2 cut(s) 205, 1036
PstNI CAGNNNCTG 1 cut(s) 647
PvuII CAGCTG 1 cut(s) 218
RsaI GTAC 2 cut(s) 320, 458
RsaNI GTAC 2 cut(s) 319, 457
SaqAI TTAA 3 cut(s) 258, 854, 1010
SatI GCNGC 8 cut(s) 39, 97, 100, 201, 210, 762, 849, 879
Sau3AI GATC 1 cut(s) 916
ScaI AGTACT 1 cut(s) 458
SchI GAGTC 1 cut(s) 895
ScrFI CCNGG 1 cut(s) 821
SfaNI GCATC 3 cut(s) 415, 970, 1015
SfcI CTRYAG 2 cut(s) 201, 1032
SmlI CTYRAG 2 cut(s) 142, 516
SmoI CTYRAG 2 cut(s) 142, 516
Sse9I AATT 6 cut(s) 114, 151, 255, 356, 666, 991
SsiI CCGC 3 cut(s) 38, 97, 209
SspMI CTAG 1 cut(s) 509
StyD4I CCNGG 1 cut(s) 819
StyI CCWWGG 1 cut(s) 677
TaaI ACNGT 3 cut(s) 332, 376, 401
TaiI ACGT 2 cut(s) 916, 1017
TaqI TCGA 2 cut(s) 9, 1017
TasI AATT 6 cut(s) 114, 151, 255, 356, 666, 991
TatI WGTACW 1 cut(s) 456
TauI GCSGC 3 cut(s) 41, 99, 212
Tru1I TTAA 3 cut(s) 258, 854, 1010
Tru9I TTAA 3 cut(s) 258, 854, 1010
TscAI CASTG 2 cut(s) 435, 470
TseFI GTSAC 1 cut(s) 500
TseI GCWGC 5 cut(s) 99, 200, 761, 848, 878
Tsp45I GTSAC 1 cut(s) 500
TspDTI ATGAA 7 cut(s) 369, 630, 713, 917, 939, 948, 1056
TspRI CASTG 2 cut(s) 435, 470
XagI CCTNNNNNAGG 1 cut(s) 707
XapI RAATTY 2 cut(s) 151, 666
XceI RCATGY 1 cut(s) 580
XmnI GAANNNNTTC 1 cut(s) 815
XspI CTAG 1 cut(s) 509
ZrmI AGTACT 1 cut(s) 458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.