Rw7G005610

General transcription factor IIE subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0001364
Physical Location & Seq
Reverse (-)
4712748 .. 4716108
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G005610.1

Sequence Viewer

Length: 1305 bp
ATGATAAAATTCTCGTTAAAATTACCCGGAGCGTATAAAATGGCTGGCAGTTTCATAAACGTAACAGAATCCCCCACCTCTCTCTCTTTCCGGAGCGGAAGTAAACACGAAAGTGACCGACTACGTAAAACCATTGAAGAGCAAAACAATGAGCATCGAGGTTTTCAACAGGTACCCCCTTCTTCAATTCCCAGGGTTTCTCCACTGGTTTTGTTTGTGAGGCTTGCGGCCAGAGCGTTCTACGATGACGTCACGCCGGAAAGTGATAATAAGCAGCCCAAGACTGGCCGGAGCGACAATAGGGGAATCGCCGTCGTGGTGATCGACGCGCTATCAAGGCGACAATGGGTGAGAGAAGAAGATCTGGCGAAGGACCTGAAACTGCACCCCAAGCAACTTCGTCGTGTTTTGCAAATGTTGATGGAGGAAAAGTTGGTGATCAGAGAACATAGGAAAGAGACAGCTAAGGGTGTGCAGATTTTCAGTGCTGCGGTTGCTGCCACAGCCAATGGGCAACAACACAGAAAAGAAGGCGAAGAGAAAATTAAGCTGCAGACTCACTCTTACTGCTGTCTGGACTATGCTCAGATATATGATGTAGTAAGGTACAGACTGCACCTTTTGAAGAAAGAACTGAAAGATGAACTGGAGGGGAAGCACGCAGTGGAGGAATATGTATGCCATAACTGCAGGAAAAGATACAACGCATTGGATGCACTGCAACTGGTTTCTATGGAGGATGAGTACTTCCACTGCGAAAGTTGCAATGGGATACTTGTGGCCGAGAGTGACAAGCTAGATTCTCAAACCACAGCAGGCCGAGACTGCAAAGCAATGCTTCAAAATATGGAGGTACAGCTGAAGCCTTTAATGGAACAAATTAATAGAGTTAAAGATTTACCATTTCCTGAATTTGGAAGTCTCCAAGCTTGGGAAGCCAGAGCAAGTGCTCCACGACTTGCAGAGAATAGTGAATCTAGTGCCATTGATCCCTCCATATCTTCTCATGGGCTGGGGTATGGTGGGACGCCCATGCCTTACGTTGGAGATACAAAGGTTGAAGTTGTGTTTTCTAAGAATGAGGACAAGGGTAAGGATATAAAATCTCAAGCTGATAATACAGACCTAAAAGTTATACCACCTTGGATGATCAAGCAAGAGATGACTCTTACAAGGGAACAGTGTGGAGAGGTCAGGCAAATGGAATCCTCACAAATGCACGTCTCCAGTCATCAGGTTCGTCGGGTTGGCATGAAGTCAGAACGGGATGAGGATGAAGGAGATGATAATATTGACTGGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03022 ko05169 ko05203 map03022 map05169 map05203
Pfam Domains
Protein Families
TFIIE_alpha

Protein Analysis

434

Amino Acids

49.45

Weight (kDa)

6.27

Isoelectric Point (pI)

48.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TFIIE_alpha PF02002 104 - 158 5.4e-08 TFIIE alpha subunit
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 252
Acc65I GGTACC 1 cut(s) 172
AccB1I GGYRCC 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 96
AccII CGCG 1 cut(s) 329
AccIII TCCGGA 1 cut(s) 90
AciI CCGC 3 cut(s) 96, 227, 491
AclWI GGATC 1 cut(s) 983
AcoI YGGCCR 3 cut(s) 228, 286, 780
AcsI RAATTY 2 cut(s) 8, 911
AcuI CTGAAG 1 cut(s) 881
AcyI GRCGYC 2 cut(s) 249, 1028
AdeI CACNNNGTG 1 cut(s) 664
AfaI GTAC 4 cut(s) 174, 608, 746, 855
AfiI CCNNNNNNNGG 4 cut(s) 284, 914, 931, 1043
AgsI TTSAA 6 cut(s) 137, 167, 186, 625, 842, 1061
AjiI CACGTC 1 cut(s) 1222
AjnI CCWGG 1 cut(s) 191
AleI CACNNNNGTG 1 cut(s) 111
AluBI AGCT 6 cut(s) 464, 550, 796, 859, 929, 1112
AluI AGCT 6 cut(s) 464, 550, 796, 859, 929, 1112
Alw21I GWGCWC 1 cut(s) 952
Alw26I GTCTC 4 cut(s) 452, 816, 926, 1228
AlwI GGATC 1 cut(s) 983
Aor13HI TCCGGA 1 cut(s) 90
AoxI GGCC 4 cut(s) 228, 286, 780, 817
ApeKI GCWGC 4 cut(s) 274, 488, 497, 550
ApoI RAATTY 2 cut(s) 8, 911
AseI ATTAAT 1 cut(s) 882
Asp718I GGTACC 1 cut(s) 172
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 373
AsuC2I CCSGG 1 cut(s) 27
AsuHPI GGTGA 3 cut(s) 331, 361, 448
AvaII GGWCC 1 cut(s) 373
BaeI ACNNNNGTAYC 2 cut(s) 1041, 1074
BanI GGYRCC 1 cut(s) 172
Bbv12I GWGCWC 1 cut(s) 952
BbvI GCAGC 4 cut(s) 286, 475, 484, 537
BccI CCATC 1 cut(s) 415
BceAI ACGGC 1 cut(s) 296
BcgI CGANNNNNNTGC 2 cut(s) 383, 417
BciT130I CCWGG 1 cut(s) 193
BciVI GTATCC 1 cut(s) 765
BclI TGATCA 2 cut(s) 438, 1149
BcnI CCSGG 1 cut(s) 27
BcoDI GTCTC 4 cut(s) 452, 816, 926, 1228
BfaI CTAG 2 cut(s) 797, 978
BfmI CTRYAG 2 cut(s) 551, 688
BfuI GTATCC 1 cut(s) 765
BglII AGATCT 1 cut(s) 361
BisI GCNGC 5 cut(s) 228, 275, 489, 498, 551
BlsI GCNGC 5 cut(s) 229, 276, 490, 499, 552
BmcAI AGTACT 1 cut(s) 746
Bme1390I CCNGG 2 cut(s) 27, 193
Bme18I GGWCC 1 cut(s) 373
BmgBI CACGTC 1 cut(s) 1222
BmgT120I GGNCC 1 cut(s) 373
BmiI GGNNCC 1 cut(s) 174
BmrFI CCNGG 2 cut(s) 27, 193
BmsI GCATC 2 cut(s) 163, 703
BpmI CTGGAG 2 cut(s) 668, 1210
Bpu10I CCTNAGC 1 cut(s) 465
BpuEI CTTGAG 1 cut(s) 1092
BpuMI CCSGG 1 cut(s) 27
BsaAI YACGTR 1 cut(s) 125
BsaHI GRCGYC 2 cut(s) 249, 1028
BsaJI CCNNGG 3 cut(s) 191, 192, 1142
BsaWI WCCGGW 1 cut(s) 90
BsaXI ACNNNNNCTCC 6 cut(s) 85, 115, 416, 446, 728, 758
Bsc4I CCNNNNNNNGG 4 cut(s) 284, 914, 931, 1043
Bse1I ACTGG 6 cut(s) 210, 289, 651, 729, 1227, 1301
Bse3DI GCAATG 2 cut(s) 772, 840
BseAI TCCGGA 1 cut(s) 90
BseBI CCWGG 1 cut(s) 193
BseDI CCNNGG 3 cut(s) 191, 192, 1142
BseGI GGATG 5 cut(s) 718, 745, 1152, 1273, 1279
BseLI CCNNNNNNNGG 4 cut(s) 284, 914, 931, 1043
BseMI GCAATG 2 cut(s) 772, 840
BseMII CTCAG 1 cut(s) 599
BseNI ACTGG 6 cut(s) 210, 289, 651, 729, 1227, 1301
BseXI GCAGC 4 cut(s) 286, 475, 484, 537
BseYI CCCAGC 1 cut(s) 1012
BsgI GTGCAG 3 cut(s) 368, 494, 599
Bsh1236I CGCG 1 cut(s) 329
BshFI GGCC 4 cut(s) 230, 288, 782, 819
BshNI GGYRCC 1 cut(s) 172
BsiHKAI GWGCWC 1 cut(s) 952
BsiSI CCGG 4 cut(s) 27, 91, 257, 289
BslFI GGGAC 1 cut(s) 1039
BslI CCNNNNNNNGG 4 cut(s) 284, 914, 931, 1043
BsmAI GTCTC 4 cut(s) 452, 816, 926, 1228
BsmBI CGTCTC 1 cut(s) 1228
BsmFI GGGAC 1 cut(s) 1039
BsnI GGCC 4 cut(s) 230, 288, 782, 819
Bsp1286I GDGCHC 1 cut(s) 952
Bsp13I TCCGGA 1 cut(s) 90
Bsp143I GATC 5 cut(s) 321, 361, 438, 988, 1149
BspACI CCGC 3 cut(s) 96, 227, 491
BspANI GGCC 4 cut(s) 230, 288, 782, 819
BspCNI CTCAG 1 cut(s) 598
BspEI TCCGGA 1 cut(s) 90
BspFNI CGCG 1 cut(s) 329
BspLI GGNNCC 1 cut(s) 174
BspMAI CTGCAG 2 cut(s) 555, 692
BspPI GGATC 1 cut(s) 983
BspQI GCTCTTC 1 cut(s) 132
BspT107I GGYRCC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 96
BsrDI GCAATG 2 cut(s) 772, 840
BsrI ACTGG 6 cut(s) 210, 289, 651, 729, 1227, 1301
BssECI CCNNGG 3 cut(s) 191, 192, 1142
BssMI GATC 5 cut(s) 321, 361, 438, 988, 1149
BssNI GRCGYC 2 cut(s) 249, 1028
BssT1I CCWWGG 1 cut(s) 1142
Bst2UI CCWGG 1 cut(s) 193
Bst4CI ACNGT 1 cut(s) 1182
Bst6I CTCTTC 2 cut(s) 132, 531
BstACI GRCGYC 2 cut(s) 249, 1028
BstAPI GCANNNNNTGC 1 cut(s) 713
BstBAI YACGTR 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 46, 225, 660, 817
BstDEI CTNAG 3 cut(s) 465, 585, 1074
BstF5I GGATG 5 cut(s) 718, 745, 1152, 1273, 1279
BstFNI CGCG 1 cut(s) 329
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 5 cut(s) 324, 364, 441, 991, 1152
BstMAI GTCTC 4 cut(s) 452, 816, 926, 1228
BstMBI GATC 5 cut(s) 321, 361, 438, 988, 1149
BstNI CCWGG 1 cut(s) 193
BstSCI CCNGG 2 cut(s) 25, 191
BstSFI CTRYAG 2 cut(s) 551, 688
BstSNI TACGTA 1 cut(s) 125
BstUI CGCG 1 cut(s) 329
BstV1I GCAGC 4 cut(s) 286, 475, 484, 537
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuI GTATCC 1 cut(s) 765
BsuRI GGCC 4 cut(s) 230, 288, 782, 819
BtrI CACGTC 1 cut(s) 1222
BtsCI GGATG 5 cut(s) 718, 745, 1152, 1273, 1279
BtsI GCAGTG 3 cut(s) 669, 716, 751
BtsIMutI CAGTG 6 cut(s) 203, 490, 669, 716, 751, 1187
Cac8I GCNNGC 4 cut(s) 46, 225, 660, 817
CfoI GCGC 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 373
CseI GACGC 2 cut(s) 335, 1036
Csp6I GTAC 4 cut(s) 173, 607, 745, 854
CspCI CAANNNNNGTGG 2 cut(s) 192, 227
CviAII CATG 3 cut(s) 1007, 1033, 1252
CviQI GTAC 4 cut(s) 173, 607, 745, 854
DdeI CTNAG 3 cut(s) 465, 585, 1074
DpnI GATC 5 cut(s) 323, 363, 440, 990, 1151
DpnII GATC 5 cut(s) 321, 361, 438, 988, 1149
DraIII CACNNNGTG 1 cut(s) 664
EaeI YGGCCR 3 cut(s) 228, 286, 780
Eam1104I CTCTTC 2 cut(s) 132, 531
EarI CTCTTC 2 cut(s) 132, 531
Eco105I TACGTA 1 cut(s) 125
Eco130I CCWWGG 1 cut(s) 1142
Eco47I GGWCC 1 cut(s) 373
Eco57I CTGAAG 1 cut(s) 881
EcoO109I RGGNCCY 1 cut(s) 373
EcoRII CCWGG 1 cut(s) 191
EcoT14I CCWWGG 1 cut(s) 1142
ErhI CCWWGG 1 cut(s) 1142
Esp3I CGTCTC 1 cut(s) 1228
FaeI CATG 3 cut(s) 1010, 1036, 1255
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FaqI GGGAC 1 cut(s) 1039
FatI CATG 3 cut(s) 1006, 1032, 1251
FbaI TGATCA 2 cut(s) 438, 1149
Fnu4HI GCNGC 5 cut(s) 228, 275, 489, 498, 551
FokI GGATG 5 cut(s) 725, 752, 1159, 1280, 1286
Fsp4HI GCNGC 5 cut(s) 228, 275, 489, 498, 551
FspBI CTAG 2 cut(s) 797, 978
GlaI GCGC 1 cut(s) 330
GluI GCNGC 5 cut(s) 228, 275, 489, 498, 551
GsaI CCCAGC 1 cut(s) 1016
GsuI CTGGAG 2 cut(s) 668, 1210
HaeIII GGCC 4 cut(s) 230, 288, 782, 819
HapII CCGG 4 cut(s) 27, 91, 257, 289
HgaI GACGC 2 cut(s) 335, 1036
HhaI GCGC 1 cut(s) 331
Hin1I GRCGYC 2 cut(s) 249, 1028
Hin1II CATG 3 cut(s) 1010, 1036, 1255
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HindIII AAGCTT 1 cut(s) 927
HinfI GANTC 7 cut(s) 68, 306, 556, 800, 974, 1165, 1205
HpaII CCGG 4 cut(s) 27, 91, 257, 289
HphI GGTGA 3 cut(s) 331, 361, 448
Hpy166II GTNNAC 1 cut(s) 104
Hpy188I TCNGA 3 cut(s) 443, 588, 1261
Hpy188III TCNNGA 3 cut(s) 91, 575, 908
Hpy8I GTNNAC 1 cut(s) 104
Hpy99I CGWCG 4 cut(s) 317, 329, 405, 1245
HpyAV CCTTC 4 cut(s) 189, 364, 524, 1271
HpyCH4III ACNGT 1 cut(s) 1182
HpyCH4IV ACGT 5 cut(s) 60, 124, 249, 1041, 1221
HpyF3I CTNAG 3 cut(s) 465, 585, 1074
HpySE526I ACGT 5 cut(s) 60, 124, 249, 1041, 1221
Hsp92I GRCGYC 2 cut(s) 249, 1028
Hsp92II CATG 3 cut(s) 1010, 1036, 1255
HspAI GCGC 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 90
KpnI GGTACC 1 cut(s) 176
Ksp22I TGATCA 2 cut(s) 438, 1149
Kzo9I GATC 5 cut(s) 321, 361, 438, 988, 1149
LguI GCTCTTC 1 cut(s) 132
LmnI GCTCC 4 cut(s) 29, 93, 291, 955
Lsp1109I GCAGC 4 cut(s) 286, 475, 484, 537
LweI GCATC 2 cut(s) 163, 703
MaeI CTAG 2 cut(s) 797, 978
MaeII ACGT 5 cut(s) 60, 124, 249, 1041, 1221
MaeIII GTNAC 4 cut(s) 61, 113, 250, 788
MalI GATC 5 cut(s) 323, 363, 440, 990, 1151
MbiI CCGCTC 1 cut(s) 96
MboI GATC 5 cut(s) 321, 361, 438, 988, 1149
MboII GAAGA 7 cut(s) 149, 174, 368, 371, 548, 637, 993
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 952
MluCI AATT 6 cut(s) 8, 20, 186, 543, 879, 911
MlyI GAGTC 2 cut(s) 550, 1159
MmeI TCCRAC 1 cut(s) 1024
MroI TCCGGA 1 cut(s) 90
MseI TTAA 5 cut(s) 17, 546, 869, 882, 891
MslI CAYNNNNRTG 1 cut(s) 111
MspA1I CMGCKG 1 cut(s) 859
MspI CCGG 4 cut(s) 27, 91, 257, 289
MspR9I CCNGG 2 cut(s) 27, 193
MvaI CCWGG 1 cut(s) 193
MvnI CGCG 1 cut(s) 329
NciI CCSGG 1 cut(s) 27
NdeII GATC 5 cut(s) 321, 361, 438, 988, 1149
NlaIII CATG 3 cut(s) 1010, 1036, 1255
NlaIV GGNNCC 1 cut(s) 174
NmeAIII GCCGAG 2 cut(s) 808, 845
NmuCI GTSAC 3 cut(s) 113, 250, 788
OliI CACNNNNGTG 1 cut(s) 111
PasI CCCWGGG 1 cut(s) 192
PciSI GCTCTTC 1 cut(s) 132
PcsI WCGNNNNNNNCGW 1 cut(s) 321
PfeI GAWTC 5 cut(s) 68, 306, 800, 974, 1205
PkrI GCNGC 5 cut(s) 229, 276, 490, 499, 552
PleI GAGTC 2 cut(s) 550, 1159
PpsI GAGTC 2 cut(s) 550, 1159
Ppu21I YACGTR 1 cut(s) 125
PpuMI RGGWCCY 1 cut(s) 373
PshBI ATTAAT 1 cut(s) 882
Psp5II RGGWCCY 1 cut(s) 373
Psp6I CCWGG 1 cut(s) 191
PspFI CCCAGC 1 cut(s) 1012
PspGI CCWGG 1 cut(s) 191
PspN4I GGNNCC 1 cut(s) 174
PspPI GGNCC 1 cut(s) 373
PspPPI RGGWCCY 1 cut(s) 373
PstI CTGCAG 2 cut(s) 555, 692
PsuI RGATCY 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 859
RsaI GTAC 4 cut(s) 174, 608, 746, 855
RsaNI GTAC 4 cut(s) 173, 607, 745, 854
RseI CAYNNNNRTG 1 cut(s) 111
SapI GCTCTTC 1 cut(s) 132
SaqAI TTAA 5 cut(s) 17, 546, 869, 882, 891
SatI GCNGC 5 cut(s) 228, 275, 489, 498, 551
Sau3AI GATC 5 cut(s) 321, 361, 438, 988, 1149
Sau96I GGNCC 1 cut(s) 373
ScaI AGTACT 1 cut(s) 746
SchI GAGTC 2 cut(s) 550, 1159
ScrFI CCNGG 2 cut(s) 27, 193
SduI GDGCHC 1 cut(s) 952
SfaNI GCATC 2 cut(s) 163, 703
SfcI CTRYAG 2 cut(s) 551, 688
SinI GGWCC 1 cut(s) 373
SmiMI CAYNNNNRTG 1 cut(s) 111
SmlI CTYRAG 1 cut(s) 1107
SmoI CTYRAG 1 cut(s) 1107
SnaBI TACGTA 1 cut(s) 125
Sse9I AATT 6 cut(s) 8, 20, 186, 543, 879, 911
SsiI CCGC 3 cut(s) 96, 227, 491
SspI AATATT 1 cut(s) 1291
SspMI CTAG 2 cut(s) 797, 978
StyD4I CCNGG 2 cut(s) 25, 191
StyI CCWWGG 1 cut(s) 1142
TaaI ACNGT 1 cut(s) 1182
TaiI ACGT 5 cut(s) 63, 127, 252, 1044, 1224
TaqI TCGA 2 cut(s) 157, 324
TaqII GACCGA 1 cut(s) 132
TasI AATT 6 cut(s) 8, 20, 186, 543, 879, 911
TatI WGTACW 1 cut(s) 744
TauI GCSGC 1 cut(s) 230
TfiI GAWTC 5 cut(s) 68, 306, 800, 974, 1205
Tru1I TTAA 5 cut(s) 17, 546, 869, 882, 891
Tru9I TTAA 5 cut(s) 17, 546, 869, 882, 891
TscAI CASTG 6 cut(s) 210, 490, 669, 723, 758, 1187
TseFI GTSAC 3 cut(s) 113, 250, 788
TseI GCWGC 4 cut(s) 274, 488, 497, 550
Tsp45I GTSAC 3 cut(s) 113, 250, 788
TspDTI ATGAA 4 cut(s) 43, 657, 1268, 1290
TspRI CASTG 6 cut(s) 210, 490, 669, 723, 758, 1187
VpaK11BI GGWCC 1 cut(s) 373
VspI ATTAAT 1 cut(s) 882
XapI RAATTY 2 cut(s) 8, 911
XspI CTAG 2 cut(s) 797, 978
ZraI GACGTC 1 cut(s) 250
ZrmI AGTACT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.