AT1G07270

cell division control

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002119
Physical Location & Seq
Reverse (-)
2229444 .. 2233017
3574 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G07270.2

Sequence Viewer

Length: 1518 bp
ATGCCTACCAACGCCGGGACAAGTTTGTCTTCTTACAAGCATATTGTAGCCATCGGAACGACGGTGAAATCCGAGAGCCTTGAATCTGCCGCATATGAGATTCCGCGGAAGAGAAAGATGAGATCGGATTCAGCGGCGGTGTCTGGTAATTCAGTCTCTACGCCGAAGAAATTGAAATCTCATCTACCAAGTTCTGTTCCCAATCCCGGAATGTCAGAAAAGGAAGTTGTGGAAGATTCTAATGAAATTCTGAGATATCCAGTGAATTTGGCTGTGTCTGATTGTTTAGGCACTAAATCTAAGTGGAGTCCTAGAGATGAAGAGCAAATGAGAGCTGTGAAAGAGGCATTGCACGTGTCCAAAGCACCATCAACAATTCTTTGCCGTGAGGACGAACAAATTCGGATTTTCGAGTTTGTGAAAGGCTGCATAGATCAGCAAAAGGCTGGGAGTTTATACATATGTGGTTGTCCTGGAACTGGAAAATCACTGTCCATGGAGAAAGTAGTACAACAAGTTGGTGATTGGTCAACTCAGGCAGGTTTGCCACCAGTGGATACATTGTCTGTGAATTGCACGTCGCTGAGTAAAACAACAGATATTTTCAGCAAGATTCTTGGTGAAATCAAGCCTGGCAAGAATGCTAACACCAACTCTTCACCTCTACAACATCTTCAGAATTTGTTTTCTCAAAAACAAGAATCTTCCAGCTCAAGAATGATGTTAATAATTGCAGATGAGATGGATTACTTAATCACAAAAGACCGAGGAGTCCTTTATGATCTTTTCATGCTTACAACTTTGCCATTTTCAAGGTGTATACTTATAGGTGTAGCAAATGCAATAGACCTTGCAGATCGTTTCCTTCCAAAATTGAAGTCCCTTAATTGCAAACCTATGGTTATAACATTCCGTGCTTATTCTAAAGATCAGATCCTTAGGATACTACAGGAGAGGCTTAGGGTTCTTTCATATGTTGCATTTCAACCAAAAGCCTTGGAGCTCTGTGCCAGAAAAGTTGCTGCAGCATCAGGTGACATGAGAAAGGCACTATGTGTCTGCAGAAGTGCTCTTGAAATCTTAGAAATAGAAACAAGAGGATCAACTGGTCCAGAATCACAAGGTCCTACTCCAGATGATTCAGTGGTGAGGATGGATCATATGGCTGCTGCATTATCTAAGACTTTTAAATCTCCTGTAGTTGAGACAATTCAGTCTCTTCCTCAACACCAACAAATCATAATATGTGCTGCTGCAAAGGCTTTCAGAGGGAGCAAAAAGGATGCAACCGTTGGAGAGCTTAATAAGTTATATCTAGAAATCTGTAAATCTTGGATGATTTCTCCAGCTGGAATCACAGAGTTCACTAACATGTGTACAGTGCTAAACGATCAGGGGATATTAAAAGTCGGTCAAGCTCGTAGGGATAAGCTAAAGAGAGTTAGCCTGAGAGTAGACGAATCAGACATCACGTTTGCTCTGCAGGAGATACGATTTTTCCGCAACTGTCTACTGTGA

Protein Analysis

505

Amino Acids

55.72

Weight (kDa)

9.01

Isoelectric Point (pI)

46.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_22 PF13401 147 - 268 4.1e-14 AAA domain
Cdc6-ORC-like_ATPase_lid PF22606 307 - 364 1.9e-10 Cdc6/ORC-like, ATPase lid domain
AAA_lid_10 PF17872 328 - 370 2.3e-06 AAA lid domain
WHD_Cdc6 PF09079 402 - 464 1.8e-14 CDC6, C terminal winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AasI GACNNNNNNGTC 3 cut(s) 25, 770, 1213
Acc36I ACCTGC 1 cut(s) 530
AccI GTMKAC 3 cut(s) 820, 1455, 1510
AccII CGCG 1 cut(s) 106
AciI CCGC 6 cut(s) 90, 104, 106, 134, 137, 1501
AclWI GGATC 3 cut(s) 928, 1108, 1164
AcsI RAATTY 4 cut(s) 246, 265, 399, 679
AcuI CTGAAG 1 cut(s) 659
AcvI CACGTG 1 cut(s) 355
AdeI CACNNNGTG 1 cut(s) 1055
AfaI GTAC 2 cut(s) 510, 1378
AfiI CCNNNNNNNGG 3 cut(s) 15, 206, 479
AflIII ACRYGT 2 cut(s) 354, 1371
AgsI TTSAA 6 cut(s) 83, 175, 813, 877, 986, 1076
AjiI CACGTC 1 cut(s) 579
AjnI CCWGG 2 cut(s) 472, 631
AluBI AGCT 7 cut(s) 335, 711, 1003, 1300, 1349, 1418, 1432
AluI AGCT 7 cut(s) 335, 711, 1003, 1300, 1349, 1418, 1432
Alw21I GWGCWC 2 cut(s) 1005, 1072
Alw26I GTCTC 3 cut(s) 160, 1199, 1221
AlwI GGATC 3 cut(s) 928, 1108, 1164
ApeKI GCWGC 7 cut(s) 426, 1022, 1025, 1166, 1169, 1250, 1253
ApoI RAATTY 4 cut(s) 246, 265, 399, 679
ArsI GACNNNNNNTTYG 4 cut(s) 863, 895, 1457, 1489
Asp700I GAANNNNTTC 1 cut(s) 399
AspS9I GGNCC 2 cut(s) 1109, 1124
AsuC2I CCSGG 2 cut(s) 16, 207
AsuHPI GGTGA 6 cut(s) 76, 533, 632, 651, 1046, 1159
AvaII GGWCC 2 cut(s) 1109, 1124
AxyI CCTNAGG 1 cut(s) 938
BanII GRGCYC 1 cut(s) 1005
BbrPI CACGTG 1 cut(s) 355
BbsI GAAGAC 1 cut(s) 21
Bbv12I GWGCWC 2 cut(s) 1005, 1072
BbvI GCAGC 7 cut(s) 413, 1009, 1037, 1153, 1156, 1237, 1240
BccI CCATC 4 cut(s) 59, 376, 736, 1147
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 2 cut(s) 474, 633
BciVI GTATCC 2 cut(s) 550, 936
BcnI CCSGG 2 cut(s) 16, 207
BcoDI GTCTC 3 cut(s) 160, 1199, 1221
BfaI CTAG 2 cut(s) 312, 1316
BfmI CTRYAG 5 cut(s) 947, 1023, 1060, 1197, 1481
BfuAI ACCTGC 1 cut(s) 530
BfuI GTATCC 2 cut(s) 550, 936
BisI GCNGC 9 cut(s) 90, 135, 427, 1023, 1026, 1167, 1170, 1251, 1254
BlsI GCNGC 9 cut(s) 91, 136, 428, 1024, 1027, 1168, 1171, 1252, 1255
Bme1390I CCNGG 4 cut(s) 16, 207, 474, 633
Bme18I GGWCC 2 cut(s) 1109, 1124
BmgBI CACGTC 1 cut(s) 579
BmgT120I GGNCC 2 cut(s) 1109, 1124
BmrFI CCNGG 4 cut(s) 16, 207, 474, 633
BmsI GCATC 2 cut(s) 1037, 1273
BpiI GAAGAC 1 cut(s) 21
BpmI CTGGAG 2 cut(s) 1116, 1329
Bpu10I CCTNAGC 1 cut(s) 959
BpuEI CTTGAG 1 cut(s) 697
BpuMI CCSGG 2 cut(s) 16, 207
BsaAI YACGTR 1 cut(s) 355
BsaJI CCNNGG 4 cut(s) 104, 495, 766, 996
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 206, 479
Bse1I ACTGG 4 cut(s) 260, 484, 551, 1111
Bse21I CCTNAGG 1 cut(s) 938
Bse3DI GCAATG 1 cut(s) 347
BseBI CCWGG 2 cut(s) 474, 633
BseDI CCNNGG 4 cut(s) 104, 495, 766, 996
BseGI GGATG 3 cut(s) 1158, 1288, 1341
BseLI CCNNNNNNNGG 3 cut(s) 15, 206, 479
BseMI GCAATG 1 cut(s) 347
BseMII CTCAG 4 cut(s) 242, 548, 575, 1439
BseNI ACTGG 4 cut(s) 260, 484, 551, 1111
BseRI GAGGAG 1 cut(s) 783
BseXI GCAGC 7 cut(s) 413, 1009, 1037, 1153, 1156, 1237, 1240
BseYI CCCAGC 1 cut(s) 446
Bsh1236I CGCG 1 cut(s) 106
BsiHKAI GWGCWC 2 cut(s) 1005, 1072
BsiSI CCGG 2 cut(s) 15, 207
BslFI GGGAC 2 cut(s) 31, 865
BslI CCNNNNNNNGG 3 cut(s) 15, 206, 479
BsmAI GTCTC 3 cut(s) 160, 1199, 1221
BsmFI GGGAC 2 cut(s) 31, 865
BsmI GAATGC 1 cut(s) 646
Bsp1286I GDGCHC 2 cut(s) 1005, 1072
Bsp1407I TGTACA 1 cut(s) 1376
Bsp143I GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 6 cut(s) 90, 104, 106, 134, 137, 1501
BspCNI CTCAG 4 cut(s) 243, 547, 576, 1440
BspFNI CGCG 1 cut(s) 106
BspMAI CTGCAG 3 cut(s) 1027, 1064, 1485
BspMI ACCTGC 1 cut(s) 530
BspPI GGATC 3 cut(s) 928, 1108, 1164
BspQI GCTCTTC 1 cut(s) 315
BsrDI GCAATG 1 cut(s) 347
BsrGI TGTACA 1 cut(s) 1376
BsrI ACTGG 4 cut(s) 260, 484, 551, 1111
BssECI CCNNGG 4 cut(s) 104, 495, 766, 996
BssMI GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
BssNAI GTATAC 1 cut(s) 821
BssT1I CCWWGG 2 cut(s) 495, 996
Bst1107I GTATAC 1 cut(s) 821
Bst2UI CCWGG 2 cut(s) 474, 633
Bst4CI ACNGT 6 cut(s) 64, 492, 1291, 1381, 1508, 1515
Bst6I CTCTTC 4 cut(s) 104, 315, 661, 1224
BstAUI TGTACA 1 cut(s) 1376
BstBAI YACGTR 1 cut(s) 355
BstDEI CTNAG 9 cut(s) 251, 300, 534, 584, 938, 959, 1081, 1179, 1448
BstDSI CCRYGG 2 cut(s) 104, 495
BstF5I GGATG 3 cut(s) 1158, 1288, 1341
BstFNI CGCG 1 cut(s) 106
BstKTI GATC 9 cut(s) 125, 436, 784, 859, 931, 936, 1103, 1159, 1393
BstMAI GTCTC 3 cut(s) 160, 1199, 1221
BstMBI GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
BstMWI GCNNNNNNNGC 1 cut(s) 1259
BstNI CCWGG 2 cut(s) 474, 633
BstNSI RCATGY 1 cut(s) 1375
BstSCI CCNGG 4 cut(s) 14, 205, 472, 631
BstSFI CTRYAG 5 cut(s) 947, 1023, 1060, 1197, 1481
BstUI CGCG 1 cut(s) 106
BstV1I GCAGC 7 cut(s) 413, 1009, 1037, 1153, 1156, 1237, 1240
BstV2I GAAGAC 1 cut(s) 21
BstX2I RGATCY 1 cut(s) 933
BstYI RGATCY 1 cut(s) 933
BstZ17I GTATAC 1 cut(s) 821
Bsu36I CCTNAGG 1 cut(s) 938
BsuI GTATCC 2 cut(s) 550, 936
BtgI CCRYGG 2 cut(s) 104, 495
BtrI CACGTC 1 cut(s) 579
BtsCI GGATG 3 cut(s) 1158, 1288, 1341
BtsIMutI CAGTG 5 cut(s) 267, 488, 558, 1149, 1386
BveI ACCTGC 1 cut(s) 530
Cfr13I GGNCC 2 cut(s) 1109, 1124
Cfr42I CCGCGG 1 cut(s) 107
Csp6I GTAC 2 cut(s) 509, 1377
CviAII CATG 4 cut(s) 496, 790, 1039, 1372
CviQI GTAC 2 cut(s) 509, 1377
DdeI CTNAG 9 cut(s) 251, 300, 534, 584, 938, 959, 1081, 1179, 1448
DpnI GATC 9 cut(s) 124, 435, 783, 858, 930, 935, 1102, 1158, 1392
DpnII GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
DraI TTTAAA 1 cut(s) 1189
DraIII CACNNNGTG 1 cut(s) 1055
DrdI GACNNNNNNGTC 3 cut(s) 25, 770, 1213
DseDI GACNNNNNNGTC 3 cut(s) 25, 770, 1213
Eam1104I CTCTTC 4 cut(s) 104, 315, 661, 1224
EarI CTCTTC 4 cut(s) 104, 315, 661, 1224
Ecl136II GAGCTC 1 cut(s) 1003
Eco130I CCWWGG 2 cut(s) 495, 996
Eco24I GRGCYC 1 cut(s) 1005
Eco32I GATATC 1 cut(s) 257
Eco47I GGWCC 2 cut(s) 1109, 1124
Eco53kI GAGCTC 1 cut(s) 1003
Eco57I CTGAAG 1 cut(s) 659
Eco72I CACGTG 1 cut(s) 355
Eco81I CCTNAGG 1 cut(s) 938
EcoICRI GAGCTC 1 cut(s) 1003
EcoO109I RGGNCCY 1 cut(s) 1124
EcoRII CCWGG 2 cut(s) 472, 631
EcoRV GATATC 1 cut(s) 257
EcoT14I CCWWGG 2 cut(s) 495, 996
EcoT38I GRGCYC 1 cut(s) 1005
ErhI CCWWGG 2 cut(s) 495, 996
FaeI CATG 4 cut(s) 499, 793, 1042, 1375
FalI AAGNNNNNCTT 2 cut(s) 13, 45
FaqI GGGAC 2 cut(s) 31, 865
FatI CATG 4 cut(s) 495, 789, 1038, 1371
FauNDI CATATG 4 cut(s) 94, 461, 973, 1161
FblI GTMKAC 3 cut(s) 820, 1455, 1510
Fnu4HI GCNGC 9 cut(s) 90, 135, 427, 1023, 1026, 1167, 1170, 1251, 1254
FokI GGATG 3 cut(s) 1165, 1295, 1348
FriOI GRGCYC 1 cut(s) 1005
Fsp4HI GCNGC 9 cut(s) 90, 135, 427, 1023, 1026, 1167, 1170, 1251, 1254
FspBI CTAG 2 cut(s) 312, 1316
GluI GCNGC 9 cut(s) 90, 135, 427, 1023, 1026, 1167, 1170, 1251, 1254
GsaI CCCAGC 1 cut(s) 450
GsuI CTGGAG 2 cut(s) 1116, 1329
HapII CCGG 2 cut(s) 15, 207
Hin1II CATG 4 cut(s) 499, 793, 1042, 1375
HincII GTYRAC 1 cut(s) 531
HindII GTYRAC 1 cut(s) 531
HpaII CCGG 2 cut(s) 15, 207
HphI GGTGA 6 cut(s) 76, 533, 632, 651, 1046, 1159
Hpy166II GTNNAC 6 cut(s) 531, 821, 1365, 1377, 1456, 1511
Hpy188III TCNNGA 5 cut(s) 714, 1073, 1112, 1133, 1316
Hpy8I GTNNAC 6 cut(s) 531, 821, 1365, 1377, 1456, 1511
Hpy99I CGWCG 2 cut(s) 64, 583
HpyAV CCTTC 1 cut(s) 875
HpyCH4III ACNGT 6 cut(s) 64, 492, 1291, 1381, 1508, 1515
HpyCH4IV ACGT 3 cut(s) 354, 578, 1472
HpyF10VI GCNNNNNNNGC 1 cut(s) 1259
HpyF3I CTNAG 9 cut(s) 251, 300, 534, 584, 938, 959, 1081, 1179, 1448
HpySE526I ACGT 3 cut(s) 354, 578, 1472
Hsp92II CATG 4 cut(s) 499, 793, 1042, 1375
KspI CCGCGG 1 cut(s) 107
Kzo9I GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
LguI GCTCTTC 1 cut(s) 315
LmnI GCTCC 2 cut(s) 1000, 1272
Lsp1109I GCAGC 7 cut(s) 413, 1009, 1037, 1153, 1156, 1237, 1240
LweI GCATC 2 cut(s) 1037, 1273
MaeI CTAG 2 cut(s) 312, 1316
MaeII ACGT 3 cut(s) 354, 578, 1472
MaeIII GTNAC 1 cut(s) 1034
MalI GATC 9 cut(s) 124, 435, 783, 858, 930, 935, 1102, 1158, 1392
MboI GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
MboII GAAGA 9 cut(s) 21, 121, 178, 245, 332, 648, 665, 696, 1211
MflI RGATCY 1 cut(s) 933
MhlI GDGCHC 2 cut(s) 1005, 1072
MlyI GAGTC 2 cut(s) 316, 780
MmeI TCCRAC 1 cut(s) 1273
MnlI CCTC 9 cut(s) 337, 382, 672, 761, 948, 1091, 1143, 1233, 1262
MroXI GAANNNNTTC 1 cut(s) 399
MseI TTAA 6 cut(s) 725, 752, 885, 1188, 1302, 1403
MslI CAYNNNNRTG 1 cut(s) 1370
MspA1I CMGCKG 3 cut(s) 106, 134, 1349
MspI CCGG 2 cut(s) 15, 207
MspR9I CCNGG 4 cut(s) 16, 207, 474, 633
Mva1269I GAATGC 1 cut(s) 646
MvaI CCWGG 2 cut(s) 474, 633
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 1259
NciI CCSGG 2 cut(s) 16, 207
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 4 cut(s) 94, 461, 973, 1161
NdeII GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
NlaIII CATG 4 cut(s) 499, 793, 1042, 1375
NmuCI GTSAC 1 cut(s) 1034
NspI RCATGY 1 cut(s) 1375
PciI ACATGT 1 cut(s) 1371
PciSI GCTCTTC 1 cut(s) 315
PctI GAATGC 1 cut(s) 646
PdmI GAANNNNTTC 1 cut(s) 399
PfoI TCCNGGA 2 cut(s) 205, 472
PkrI GCNGC 9 cut(s) 91, 136, 428, 1024, 1027, 1168, 1171, 1252, 1255
PleI GAGTC 2 cut(s) 315, 779
PmaCI CACGTG 1 cut(s) 355
PmlI CACGTG 1 cut(s) 355
PpsI GAGTC 2 cut(s) 315, 779
Ppu21I YACGTR 1 cut(s) 355
PpuMI RGGWCCY 1 cut(s) 1124
PscI ACATGT 1 cut(s) 1371
PsiI TTATAA 1 cut(s) 905
Psp124BI GAGCTC 1 cut(s) 1005
Psp5II RGGWCCY 1 cut(s) 1124
Psp6I CCWGG 2 cut(s) 472, 631
PspCI CACGTG 1 cut(s) 355
PspFI CCCAGC 1 cut(s) 446
PspGI CCWGG 2 cut(s) 472, 631
PspPI GGNCC 2 cut(s) 1109, 1124
PspPPI RGGWCCY 1 cut(s) 1124
PstI CTGCAG 3 cut(s) 1027, 1064, 1485
PsuI RGATCY 1 cut(s) 933
PvuII CAGCTG 1 cut(s) 1349
RsaI GTAC 2 cut(s) 510, 1378
RsaNI GTAC 2 cut(s) 509, 1377
RseI CAYNNNNRTG 1 cut(s) 1370
SacI GAGCTC 1 cut(s) 1005
SacII CCGCGG 1 cut(s) 107
SapI GCTCTTC 1 cut(s) 315
SaqAI TTAA 6 cut(s) 725, 752, 885, 1188, 1302, 1403
SatI GCNGC 9 cut(s) 90, 135, 427, 1023, 1026, 1167, 1170, 1251, 1254
Sau3AI GATC 9 cut(s) 122, 433, 781, 856, 928, 933, 1100, 1156, 1390
Sau96I GGNCC 2 cut(s) 1109, 1124
SchI GAGTC 2 cut(s) 316, 780
ScrFI CCNGG 4 cut(s) 16, 207, 474, 633
SduI GDGCHC 2 cut(s) 1005, 1072
SfaNI GCATC 2 cut(s) 1037, 1273
SfcI CTRYAG 5 cut(s) 947, 1023, 1060, 1197, 1481
Sfr303I CCGCGG 1 cut(s) 107
SgrBI CCGCGG 1 cut(s) 107
SinI GGWCC 2 cut(s) 1109, 1124
SmiMI CAYNNNNRTG 1 cut(s) 1370
SmlI CTYRAG 1 cut(s) 712
SmoI CTYRAG 1 cut(s) 712
SsiI CCGC 6 cut(s) 90, 104, 106, 134, 137, 1501
SspMI CTAG 2 cut(s) 312, 1316
SstI GAGCTC 1 cut(s) 1005
StyD4I CCNGG 4 cut(s) 14, 205, 472, 631
StyI CCWWGG 2 cut(s) 495, 996
TaaI ACNGT 6 cut(s) 64, 492, 1291, 1381, 1508, 1515
TaiI ACGT 3 cut(s) 357, 581, 1475
TaqI TCGA 1 cut(s) 411
TaqII GACCGA 2 cut(s) 780, 1400
TatI WGTACW 2 cut(s) 508, 1376
TauI GCSGC 2 cut(s) 92, 137
Tru1I TTAA 6 cut(s) 725, 752, 885, 1188, 1302, 1403
Tru9I TTAA 6 cut(s) 725, 752, 885, 1188, 1302, 1403
TscAI CASTG 5 cut(s) 267, 495, 558, 1149, 1386
TseFI GTSAC 1 cut(s) 1034
TseI GCWGC 7 cut(s) 426, 1022, 1025, 1166, 1169, 1250, 1253
Tsp45I GTSAC 1 cut(s) 1034
TspDTI ATGAA 4 cut(s) 258, 333, 778, 960
TspGWI ACGGA 1 cut(s) 902
TspRI CASTG 5 cut(s) 267, 495, 558, 1149, 1386
VpaK11BI GGWCC 2 cut(s) 1109, 1124
XapI RAATTY 4 cut(s) 246, 265, 399, 679
XbaI TCTAGA 1 cut(s) 1315
XceI RCATGY 1 cut(s) 1375
XmiI GTMKAC 3 cut(s) 820, 1455, 1510
XmnI GAANNNNTTC 1 cut(s) 399
XspI CTAG 2 cut(s) 312, 1316
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.