MD03G1072700.v1.1

Cell division control protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002119
Physical Location & Seq
Reverse (-)
5899713 .. 5903813
4101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1072700.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGCCTGCCATCGCCAAACGCAGCCTCGAGCTCAACGACGACAGCATGCCCGTCCAATGGGAGAGTACGCCCGATGCTGTCAGATCCGCCGGAGATTCGACACCTCCGAGGCGGAGGCTCAGATCCAACTCGTCGCCGATGAAGCTGAGCCCGATCTCCAAGCCCATGAAATTGGAATCGCCCCGAAAGCGGCTAAGTGCTTGCGCCAACACTCAGGCAAATGGAATTGAGAAGTATTTCACCCAGAAGAAGGTTCAGACTGTGTGCAAACCAGTCGAAAAATCAGTTGAGAAACTGAAATGGAACCCGAAAGATGGCAAGCAAATCAGCGAGGTGAAGGAGGCATTGCATGTTTCAACCGTGCCGCCAACTGTTTCATGCCGGGAAGATGAGCAGAAGAGGGTTTTGGAGTTCTTGAAGCAATGCATAGAGCAAGAGAAAGCTGGGAGTTTGTACGTGTGCGGGTGTCCCGGGACCGGAAAATCGCTGTCCATTGAGAAAGTGAAGCAGGCTTTGGTTGATTGGGCGGGGGAGGCCGGATTTGAGGAACCGGATGTGTTGGCTTTGAATTGTTCTTCTCTAACAAAGACTTCTGATATTTTCACTAAGATACTTGGTAAAGACCAACCGCCGAAGAAAGTTAAAAGCAAAACCTCATCCCTGCAGCTTCTACAAAATTTGTACTCCCAGAAGCCTTCGTCTTCTAGAAGGAAGATGACGCTGGTAATAGCTGATGAATTGGACTTCTTAATTACTAAAGACCGGGCGGTGCTTCATGATCTTTTTATGCTCACAACGTACCAATTTTCGAGATGTATACTCATAGGAGTGGCAAATGCCATAGACCTAGCCGATCGTTTTCTTCCAAGACTTCAGTCACTAAATTGCAAACCTATCGTTATTACTTTTCGGGCGTACTCTTATGACCAGATTCTCAGGATTCTTCAGCAGAGGCTAATAACACTACCTTACATCGTCTTTCAACCACCAGCATTGGAACTTTGTGCCAGAAAAGTTGCGGCTGCATCTGGAGATATGAGGAAAGCACTTTGTATTTGCAGGAGTGCAATTGAGATTCTGGAAGCTGAGGTGAAAGAATCTACTGATAACTTGAATTCATCACCAGTAGTGGACGCATTCGTTGAGCAGCAGACGGCTCCTGCTCCAGCTCCTGTTGGGAAAAAACAGGAGATTGATGTTGTGACAATTGGTCATATGGCTTCTGCTTTGTCAAAGACATTCAAATCACCAGTAGTGGACACCATACAAAGTCTTCCGCAACATCAACAGATTATACTTTGCTCTGCCGTGAGGCTTTTCCGTGGAAAAAAGAAGGATTCAACTGTAGCACAGCTAAATAAATCTTATATAGACATCTGCAAATCAACACTAATCCCACCAGTCGGAATTTTTGAATTTTCAAGTATGTGCAGAGTGCTAAATGACCAGGGGCTCCTGAAATTGGGCGGTCAATCTCGAGATGAAAAACTTAAAAGAGTGACAATGAATGCAGCAGATGAAGCAGACATCACTTTTGCATTACAGGGAATTCGTTTCTTTCGGAACTGTCTTCAGTAG

Protein Analysis

526

Amino Acids

58.36

Weight (kDa)

9.27

Isoelectric Point (pI)

54.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AAA_16 PF13191 126 - 262 1e-05 AAA ATPase domain
AAA_22 PF13401 147 - 266 7.4e-12 AAA domain
Cdc6-ORC-like_ATPase_lid PF22606 306 - 363 7.1e-11 Cdc6/ORC-like, ATPase lid domain
AAA_lid_10 PF17872 329 - 362 8.3e-06 AAA lid domain
WHD_Cdc6 PF09079 420 - 509 5.3e-18 CDC6, C terminal winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 817
AclWI GGATC 2 cut(s) 78, 117
AcsI RAATTY 5 cut(s) 676, 1114, 1407, 1415, 1548
AcuI CTGAAG 3 cut(s) 857, 929, 1556
AfaI GTAC 5 cut(s) 67, 455, 683, 800, 917
AfiI CCNNNNNNNGG 7 cut(s) 57, 189, 250, 314, 476, 1403, 1462
AflIII ACRYGT 1 cut(s) 456
AgsI TTSAA 9 cut(s) 357, 418, 568, 983, 1114, 1243, 1341, 1415, 1422
AhdI GACNNNNNGTC 1 cut(s) 1209
AjnI CCWGG 1 cut(s) 1446
AjuI GAANNNNNNNTTGG 4 cut(s) 389, 421, 497, 529
AluBI AGCT 8 cut(s) 31, 145, 443, 667, 731, 1085, 1169, 1354
AluI AGCT 8 cut(s) 31, 145, 443, 667, 731, 1085, 1169, 1354
Alw21I GWGCWC 1 cut(s) 33
AlwI GGATC 2 cut(s) 78, 117
AlwNI CAGNNNCTG 1 cut(s) 1172
Ama87I CYCGRG 3 cut(s) 26, 470, 1476
AoxI GGCC 1 cut(s) 534
ApeKI GCWGC 5 cut(s) 21, 664, 1022, 1147, 1511
ApoI RAATTY 5 cut(s) 676, 1114, 1407, 1415, 1548
ArsI GACNNNNNNTTYG 2 cut(s) 1517, 1549
Asp700I GAANNNNTTC 1 cut(s) 236
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 1 cut(s) 474
AsuC2I CCSGG 4 cut(s) 383, 471, 472, 764
AsuHPI GGTGA 5 cut(s) 232, 346, 1102, 1113, 1239
AvaI CYCGRG 3 cut(s) 26, 470, 1476
AvaII GGWCC 1 cut(s) 474
BanII GRGCYC 3 cut(s) 33, 152, 1455
BbsI GAAGAC 3 cut(s) 693, 1265, 1562
Bbv12I GWGCWC 1 cut(s) 33
BbvCI CCTCAGC 1 cut(s) 1086
BbvI GCAGC 5 cut(s) 33, 676, 1009, 1159, 1523
BccI CCATC 2 cut(s) 17, 308
BceAI ACGGC 2 cut(s) 1170, 1292
BcgI CGANNNNNNTGC 4 cut(s) 256, 290, 877, 911
BciT130I CCWGG 1 cut(s) 1448
BcnI CCSGG 4 cut(s) 383, 471, 472, 764
BfaI CTAG 2 cut(s) 705, 848
BfmI CTRYAG 2 cut(s) 662, 1344
BisI GCNGC 8 cut(s) 22, 191, 365, 665, 1020, 1023, 1148, 1512
BlpI GCTNAGC 1 cut(s) 146
BlsI GCNGC 8 cut(s) 23, 192, 366, 666, 1021, 1024, 1149, 1513
Bme1390I CCNGG 5 cut(s) 383, 471, 472, 764, 1448
Bme18I GGWCC 1 cut(s) 474
BmeRI GACNNNNNGTC 1 cut(s) 1209
BmeT110I CYCGRG 3 cut(s) 26, 470, 1476
BmgT120I GGNCC 1 cut(s) 474
BmiI GGNNCC 5 cut(s) 305, 475, 549, 1158, 1454
BmrFI CCNGG 5 cut(s) 383, 471, 472, 764, 1448
BmsI GCATC 2 cut(s) 64, 1034
BoxI GACNNNNGTC 1 cut(s) 874
BpiI GAAGAC 3 cut(s) 693, 1265, 1562
BpmI CTGGAG 2 cut(s) 1050, 1149
Bpu10I CCTNAGC 1 cut(s) 1086
Bpu1102I GCTNAGC 1 cut(s) 146
BpuMI CCSGG 4 cut(s) 383, 471, 472, 764
BsaAI YACGTR 1 cut(s) 457
BsaJI CCNNGG 4 cut(s) 107, 470, 1321, 1447
BsaWI WCCGGW 2 cut(s) 476, 550
Bsc4I CCNNNNNNNGG 7 cut(s) 57, 189, 250, 314, 476, 1403, 1462
Bse1I ACTGG 4 cut(s) 272, 1124, 1250, 1400
Bse3DI GCAATG 2 cut(s) 344, 428
BseBI CCWGG 1 cut(s) 1448
BseDI CCNNGG 4 cut(s) 107, 470, 1321, 1447
BseGI GGATG 2 cut(s) 559, 656
BseLI CCNNNNNNNGG 7 cut(s) 57, 189, 250, 314, 476, 1403, 1462
BseMI GCAATG 2 cut(s) 344, 428
BseMII CTCAG 5 cut(s) 133, 137, 227, 949, 1077
BseNI ACTGG 4 cut(s) 272, 1124, 1250, 1400
BseXI GCAGC 5 cut(s) 33, 676, 1009, 1159, 1523
BseYI CCCAGC 1 cut(s) 443
BsgI GTGCAG 1 cut(s) 1450
Bsh1285I CGRYCG 1 cut(s) 856
BshFI GGCC 1 cut(s) 536
BsiEI CGRYCG 1 cut(s) 856
BsiHKAI GWGCWC 1 cut(s) 33
BsiHKCI CYCGRG 3 cut(s) 26, 470, 1476
BsiSI CCGG 7 cut(s) 90, 382, 471, 477, 537, 551, 763
BslFI GGGAC 2 cut(s) 453, 487
BslI CCNNNNNNNGG 7 cut(s) 57, 189, 250, 314, 476, 1403, 1462
BsmFI GGGAC 2 cut(s) 453, 487
BsmI GAATGC 2 cut(s) 1136, 1513
BsnI GGCC 1 cut(s) 536
BsoBI CYCGRG 3 cut(s) 26, 470, 1476
Bsp1286I GDGCHC 3 cut(s) 33, 152, 1455
Bsp143I GATC 5 cut(s) 83, 122, 153, 778, 853
Bsp1720I GCTNAGC 1 cut(s) 146
BspANI GGCC 1 cut(s) 536
BspCNI CTCAG 5 cut(s) 132, 138, 226, 948, 1078
BspHI TCATGA 1 cut(s) 775
BspLI GGNNCC 5 cut(s) 305, 475, 549, 1158, 1454
BspMAI CTGCAG 1 cut(s) 666
BspPI GGATC 2 cut(s) 78, 117
BsrDI GCAATG 2 cut(s) 344, 428
BsrI ACTGG 4 cut(s) 272, 1124, 1250, 1400
BssECI CCNNGG 4 cut(s) 107, 470, 1321, 1447
BssMI GATC 5 cut(s) 83, 122, 153, 778, 853
BssNAI GTATAC 1 cut(s) 818
Bst1107I GTATAC 1 cut(s) 818
Bst2UI CCWGG 1 cut(s) 1448
Bst4CI ACNGT 5 cut(s) 262, 361, 373, 1345, 1568
Bst6I CTCTTC 1 cut(s) 392
BstBAI YACGTR 1 cut(s) 457
BstC8I GCNNGC 5 cut(s) 6, 47, 202, 320, 510
BstDEI CTNAG 7 cut(s) 119, 146, 194, 213, 606, 935, 1086
BstDSI CCRYGG 1 cut(s) 1321
BstF5I GGATG 2 cut(s) 559, 656
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 5 cut(s) 86, 125, 156, 781, 856
BstMBI GATC 5 cut(s) 83, 122, 153, 778, 853
BstMCI CGRYCG 1 cut(s) 856
BstMWI GCNNNNNNNGC 4 cut(s) 142, 187, 533, 1520
BstNI CCWGG 1 cut(s) 1448
BstNSI RCATGY 2 cut(s) 49, 353
BstPAI GACNNNNGTC 1 cut(s) 874
BstSCI CCNGG 5 cut(s) 381, 469, 470, 762, 1446
BstSFI CTRYAG 2 cut(s) 662, 1344
BstV1I GCAGC 5 cut(s) 33, 676, 1009, 1159, 1523
BstV2I GAAGAC 3 cut(s) 693, 1265, 1562
BstX2I RGATCY 2 cut(s) 83, 122
BstXI CCANNNNNNTGG 1 cut(s) 172
BstYI RGATCY 2 cut(s) 83, 122
BstZ17I GTATAC 1 cut(s) 818
BsuRI GGCC 1 cut(s) 536
BtgI CCRYGG 1 cut(s) 1321
BtsCI GGATG 2 cut(s) 559, 656
Cac8I GCNNGC 5 cut(s) 6, 47, 202, 320, 510
CaiI CAGNNNCTG 1 cut(s) 1172
CciI TCATGA 1 cut(s) 775
CfoI GCGC 1 cut(s) 206
Cfr13I GGNCC 1 cut(s) 474
Cfr9I CCCGGG 1 cut(s) 470
CseI GACGC 2 cut(s) 727, 1142
Csp6I GTAC 5 cut(s) 66, 454, 682, 799, 916
CspCI CAANNNNNGTGG 2 cut(s) 975, 1010
CviAII CATG 5 cut(s) 46, 166, 350, 378, 776
CviQI GTAC 5 cut(s) 66, 454, 682, 799, 916
DdeI CTNAG 7 cut(s) 119, 146, 194, 213, 606, 935, 1086
DpnI GATC 5 cut(s) 85, 124, 155, 780, 855
DpnII GATC 5 cut(s) 83, 122, 153, 778, 853
DriI GACNNNNNGTC 1 cut(s) 1209
Eam1104I CTCTTC 1 cut(s) 392
Eam1105I GACNNNNNGTC 1 cut(s) 1209
EarI CTCTTC 1 cut(s) 392
EciI GGCGGA 2 cut(s) 76, 127
Ecl136II GAGCTC 1 cut(s) 31
Eco24I GRGCYC 3 cut(s) 33, 152, 1455
Eco47I GGWCC 1 cut(s) 474
Eco53kI GAGCTC 1 cut(s) 31
Eco57I CTGAAG 3 cut(s) 857, 929, 1556
Eco88I CYCGRG 3 cut(s) 26, 470, 1476
EcoICRI GAGCTC 1 cut(s) 31
EcoRI GAATTC 2 cut(s) 1114, 1548
EcoRII CCWGG 1 cut(s) 1446
EcoT22I ATGCAT 1 cut(s) 428
EcoT38I GRGCYC 3 cut(s) 33, 152, 1455
FaeI CATG 5 cut(s) 49, 169, 353, 381, 779
FaqI GGGAC 2 cut(s) 453, 487
FatI CATG 5 cut(s) 45, 165, 349, 377, 775
FauI CCCGC 2 cut(s) 455, 520
FauNDI CATATG 1 cut(s) 1215
FblI GTMKAC 1 cut(s) 817
Fnu4HI GCNGC 8 cut(s) 22, 191, 365, 665, 1020, 1023, 1148, 1512
FokI GGATG 2 cut(s) 566, 643
FriOI GRGCYC 3 cut(s) 33, 152, 1455
Fsp4HI GCNGC 8 cut(s) 22, 191, 365, 665, 1020, 1023, 1148, 1512
FspBI CTAG 2 cut(s) 705, 848
GlaI GCGC 1 cut(s) 205
GluI GCNGC 8 cut(s) 22, 191, 365, 665, 1020, 1023, 1148, 1512
GsaI CCCAGC 1 cut(s) 447
GsuI CTGGAG 2 cut(s) 1050, 1149
HaeIII GGCC 1 cut(s) 536
HapII CCGG 7 cut(s) 90, 382, 471, 477, 537, 551, 763
HgaI GACGC 2 cut(s) 727, 1142
HhaI GCGC 1 cut(s) 206
Hin1II CATG 5 cut(s) 49, 169, 353, 381, 779
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HinfI GANTC 7 cut(s) 95, 176, 931, 940, 1075, 1097, 1337
HpaII CCGG 7 cut(s) 90, 382, 471, 477, 537, 551, 763
HphI GGTGA 5 cut(s) 232, 346, 1102, 1113, 1239
Hpy166II GTNNAC 3 cut(s) 818, 1132, 1258
Hpy188I TCNGA 7 cut(s) 83, 108, 122, 258, 595, 1406, 1563
Hpy8I GTNNAC 3 cut(s) 818, 1132, 1258
Hpy99I CGWCG 2 cut(s) 41, 136
HpyAV CCTTC 5 cut(s) 244, 331, 702, 705, 1327
HpyCH4III ACNGT 5 cut(s) 262, 361, 373, 1345, 1568
HpyCH4IV ACGT 2 cut(s) 456, 797
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 187, 533, 1520
HpyF3I CTNAG 7 cut(s) 119, 146, 194, 213, 606, 935, 1086
HpySE526I ACGT 2 cut(s) 456, 797
Hsp92II CATG 5 cut(s) 49, 169, 353, 381, 779
HspAI GCGC 1 cut(s) 204
Kzo9I GATC 5 cut(s) 83, 122, 153, 778, 853
LmnI GCTCC 4 cut(s) 1162, 1168, 1174, 1458
Lsp1109I GCAGC 5 cut(s) 33, 676, 1009, 1159, 1523
LweI GCATC 2 cut(s) 64, 1034
MaeI CTAG 2 cut(s) 705, 848
MaeII ACGT 2 cut(s) 456, 797
MaeIII GTNAC 3 cut(s) 876, 1201, 1498
MalI GATC 5 cut(s) 85, 124, 155, 780, 855
MboI GATC 5 cut(s) 83, 122, 153, 778, 853
MfeI CAATTG 2 cut(s) 1068, 1206
MflI RGATCY 2 cut(s) 83, 122
MhlI GDGCHC 3 cut(s) 33, 152, 1455
MmeI TCCRAC 2 cut(s) 150, 1384
Mph1103I ATGCAT 1 cut(s) 428
MroXI GAANNNNTTC 1 cut(s) 236
MseI TTAA 3 cut(s) 642, 749, 1491
MslI CAYNNNNRTG 1 cut(s) 827
MspI CCGG 7 cut(s) 90, 382, 471, 477, 537, 551, 763
MspR9I CCNGG 5 cut(s) 383, 471, 472, 764, 1448
MunI CAATTG 2 cut(s) 1068, 1206
Mva1269I GAATGC 2 cut(s) 1136, 1513
MvaI CCWGG 1 cut(s) 1448
MwoI GCNNNNNNNGC 4 cut(s) 142, 187, 533, 1520
NciI CCSGG 4 cut(s) 383, 471, 472, 764
NdeI CATATG 1 cut(s) 1215
NdeII GATC 5 cut(s) 83, 122, 153, 778, 853
NlaIII CATG 5 cut(s) 49, 169, 353, 381, 779
NlaIV GGNNCC 5 cut(s) 305, 475, 549, 1158, 1454
NmuCI GTSAC 3 cut(s) 876, 1201, 1498
NsiI ATGCAT 1 cut(s) 428
NspI RCATGY 2 cut(s) 49, 353
PaeI GCATGC 1 cut(s) 49
PaeR7I CTCGAG 2 cut(s) 26, 1476
PagI TCATGA 1 cut(s) 775
PcsI WCGNNNNNNNCGW 2 cut(s) 33, 104
PctI GAATGC 2 cut(s) 1136, 1513
PdmI GAANNNNTTC 1 cut(s) 236
PfeI GAWTC 7 cut(s) 95, 176, 931, 940, 1075, 1097, 1337
PkrI GCNGC 8 cut(s) 23, 192, 366, 666, 1021, 1024, 1149, 1513
Ple19I CGATCG 1 cut(s) 856
Ppu21I YACGTR 1 cut(s) 457
PshAI GACNNNNGTC 1 cut(s) 874
Psp124BI GAGCTC 1 cut(s) 33
Psp6I CCWGG 1 cut(s) 1446
PspFI CCCAGC 1 cut(s) 443
PspGI CCWGG 1 cut(s) 1446
PspN4I GGNNCC 5 cut(s) 305, 475, 549, 1158, 1454
PspPI GGNCC 1 cut(s) 474
PspXI VCTCGAGB 1 cut(s) 26
PstI CTGCAG 1 cut(s) 666
PstNI CAGNNNCTG 1 cut(s) 1172
PsuI RGATCY 2 cut(s) 83, 122
PvuI CGATCG 1 cut(s) 856
RsaI GTAC 5 cut(s) 67, 455, 683, 800, 917
RsaNI GTAC 5 cut(s) 66, 454, 682, 799, 916
RseI CAYNNNNRTG 1 cut(s) 827
SacI GAGCTC 1 cut(s) 33
SaqAI TTAA 3 cut(s) 642, 749, 1491
SatI GCNGC 8 cut(s) 22, 191, 365, 665, 1020, 1023, 1148, 1512
Sau3AI GATC 5 cut(s) 83, 122, 153, 778, 853
Sau96I GGNCC 1 cut(s) 474
ScrFI CCNGG 5 cut(s) 383, 471, 472, 764, 1448
SduI GDGCHC 3 cut(s) 33, 152, 1455
SfaNI GCATC 2 cut(s) 64, 1034
SfcI CTRYAG 2 cut(s) 662, 1344
Sfr274I CTCGAG 2 cut(s) 26, 1476
SinI GGWCC 1 cut(s) 474
SlaI CTCGAG 2 cut(s) 26, 1476
SmaI CCCGGG 1 cut(s) 472
SmiMI CAYNNNNRTG 1 cut(s) 827
SmlI CTYRAG 2 cut(s) 26, 1476
SmoI CTYRAG 2 cut(s) 26, 1476
SphI GCATGC 1 cut(s) 49
SspMI CTAG 2 cut(s) 705, 848
SstI GAGCTC 1 cut(s) 33
StyD4I CCNGG 5 cut(s) 381, 469, 470, 762, 1446
TaaI ACNGT 5 cut(s) 262, 361, 373, 1345, 1568
TaiI ACGT 2 cut(s) 459, 800
TaqI TCGA 5 cut(s) 27, 98, 276, 809, 1477
TatI WGTACW 1 cut(s) 681
TauI GCSGC 3 cut(s) 193, 367, 1022
TfiI GAWTC 7 cut(s) 95, 176, 931, 940, 1075, 1097, 1337
Tru1I TTAA 3 cut(s) 642, 749, 1491
Tru9I TTAA 3 cut(s) 642, 749, 1491
TseFI GTSAC 3 cut(s) 876, 1201, 1498
TseI GCWGC 5 cut(s) 21, 664, 1022, 1147, 1511
Tsp45I GTSAC 3 cut(s) 876, 1201, 1498
TspDTI ATGAA 9 cut(s) 155, 182, 366, 750, 764, 1107, 1497, 1520, 1533
TspGWI ACGGA 1 cut(s) 1310
TspMI CCCGGG 1 cut(s) 470
VpaK11BI GGWCC 1 cut(s) 474
XapI RAATTY 5 cut(s) 676, 1114, 1407, 1415, 1548
XbaI TCTAGA 1 cut(s) 704
XceI RCATGY 2 cut(s) 49, 353
XhoI CTCGAG 2 cut(s) 26, 1476
XmaI CCCGGG 1 cut(s) 470
XmiI GTMKAC 1 cut(s) 817
XmnI GAANNNNTTC 1 cut(s) 236
XspI CTAG 2 cut(s) 705, 848
Zsp2I ATGCAT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.