AT1G27940

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001951
Physical Location & Seq
Reverse (-)
9733430 .. 9738236
4807 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G27940.2

Sequence Viewer

Length: 3738 bp
ATGGATAACACAGAAAGATCCTCTAATGGAAACATCCAAGCCGAGACGGAAGCAAAGGAGGAGAAGAAAAATATAAAGAAGGAATCAGTGTCATTGATGGGTTTGTTTAGCGCAGCAGATAAACTTGATTACTTTCTCATGTTGCTAGGAGGTTTAGGTGCGTGTATACATGGCGCTACGCTTCCTCTCTTCTTCGTGTTCTTCGGAAAAATGCTAGATTCTCTTGGAAATCTTTCTACAGATCCTAAAGCCATATCTTCCCGCGTTTCACAGAATGCTCTGTACTTGGTCTACTTAGGATTGGTCAATTTTGTATCAGCTTGGATCGGAGTTTCTTGTTGGATGCAAACAGGGGAAAGACAAACAGCACGATTGCGTATAAATTATCTCAAATCGATATTAGCAAAAGACATTACTTTCTTTGATACTGAAGCTAGAGATTCAAATCTCATTTTTCACATATCAAGCGATGCTATCTTGGTTCAAGATGCAATTGGCGATAAGACCGACCATGTTTTGCGGTATCTATCTCAGTTTATAGCGGGATTTGTCATTGGTTTTTTGTCGGTATGGCAACTAACGCTGCTCACGTTAGGAGTGGTTCCACTGATAGCTATTGCAGGAGGAGGGTATGCTATAGTCATGTCTACAATCTCAGAGAAGAGTGAGACTGCTTATGCTGATGCAGGAAAAGTCGCAGAAGAGGTTATGTCACAGGTGAGAACAGTGTATGCTTTTGTAGGAGAAGAGAAAGCTGTAAAATCTTACTCAAATTCTCTCAAGAAAGCTCTGAAACTCGGTAAAAGGAGCGGTCTTGCTAAAGGTTTAGGAGTTGGATTAACATATAGCCTCTTGTTCTGTGCTTGGGCTTTGCTTTTGTGGTATGCAAGTCTTCTTGTACGCCACGGCAAAACCAATGGTGCAAAAGCCTTCACAACCATCCTCAATGTCATTTTCAGTGGATTCGCCTTAGGTCAAGCCGCACCTAGCTTATCCGCGATTGCTAAGGGTAGAGTTGCTGCTGCAAACATATTCAGAATGATAGGGAACAACAATTCTGAAAGTTCTCAAAGATTAGACGAGGGAACAACATTGCAAAATGTAGCTGGGAGGATTGAGTTTCAAAAAGTGTCTTTCGCATATCCTTCTAGACCAAACATGGTTTTTGAGAATCTTAGTTTCACAATACGGTCAGGCAAGACTTTTGCATTTGTCGGTCCAAGTGGTTCAGGGAAAAGCACAATCATATCAATGGTTCAACGTTTCTATGAACCTAACTCAGGGGAGATTCTCTTAGATGGAAATGACATCAAGAGCCTAAAGCTTAAATGGTTTAGAGAACAGCTTGGATTAGTGAGTCAAGAACCAGCATTATTCGCCACAACAATAGCTTCCAATATTCTTTTAGGTAAAGAGAATGCGAATATGGATCAGATCATAGAAGCTGCTAAAGCAGCTAATGCTGATTCTTTCATTAAATCATTACCTAATGGTTACAATACTCAGGTTGGAGAAGGAGGAACTCAACTCTCAGGAGGACAGAAACAGAGGATTGCAATTGCTCGAGCAGTTCTAAGAAATCCAAAGATATTACTTTTAGATGAAGCAACAAGCGCTCTTGATGCCGAATCAGAGAAGATTGTTCAGCAAGCACTTGACAATGTCATGGAAAAAAGAACAACAATCGTCGTTGCACATAGATTATCCACCATTCGAAATGTTGACAAGATTGTTGTCTTGAGAGATGGTCAAGTTAGGGAAACCGGAAGCCACTCAGAACTCATGTTAAGAGGAGGAGATTATGCGACTCTTGTCAATTGCCAAGAGACAGAACCTCAAGAAAACTCGAGATCAATTATGTCTGAAACTTGTAAATCTCAAGCTGGATCTTCTAGTTCAAGAAGAGTTTCTAGCTCAAGGAGGACTTCAAGCTTCAGGGTAGACCAAGAGAAGACTAAAAATGATGATTCCAAAAAAGACTTTAGCTCTTCTTCGATGATATGGGAACTTATAAAGTTGAATTCTCCAGAATGGCCATATGCATTACTTGGCTCCATTGGTGCAGTACTTGCTGGAGCACAAACTCCATTGTTCTCTATGGGGATTGCTTATGTGTTAACTGCATTTTATTCGCCTTTTCCTAATGTGATCAAGCGCGACGTAGAAAAGGTAGCTATTATCTTTGCTGGAGCTGGAATAGTAACTGCACCTATATATCTTCTCCAACATTACTTCTATACACTAATGGGAGAGCGTCTTACTTCTAGGGTTCGCTTATCCTTGTTCTCAGCGATTCTTTCAAATGAGATTGGATGGTTTGATCTGGATGAGAACAACACTGGCTCACTTACTTCAATCTTAGCTGCTGATGCAACTTTGGTGAGGAGTGCTCTAGCGGATCGCCTCTCGACAATAGTCCAAAACTTGTCTCTTACAGTCACTGCATTGGCACTTGCCTTTTTCTATAGTTGGCGTGTTGCAGCTGTGGTAACGGCTTGTTTCCCTCTTCTCATTGCGGCTTCACTTACCGAGCAACTATTTTTAAAAGGCTTTGGAGGAGATTACACAAGAGCTTACTCTAGGGCAACTTCAGTGGCACGTGAAGCGATTGCAAATATAAGAACTGTCGCTGCATATGGTGCCGAAAAGCAGATCTCGGAACAGTTTACTTGTGAGCTAAGCAAGCCCACCAAGAATGCCTTTGTCAGAGGTCATATCTCTGGATTTGGATACGGTTTGTCTCAGTTTCTTGCGTTCTGTTCCTACGCTCTTGGTCTCTGGTACGTATCGGTTTTGATCAATCATAAGGAGACGAATTTTGGGGATAGTATCAAATCCTTCATGGTGTTGATCGTCACAGCTTTCTCAGTTTCGGAAACGCTTGCTTTGACCCCAGATATCGTGAAGGGCACGCAAGCACTCGGGTCAGTTTTCAGGGTTTTACATAGAGAGACCAAGATATCTCCAGACCAGCCTAACTCAAGAATGGTTAGTCAAGTCAAAGGAGATATAGAGTTTAGAAACGTGAGTTTTGTGTATCCCACAAGACCCGAAATTGACATTTTCAAAAACCTAAACCTCCGAGTTTCAGCCGGGAAAAGTCTTGCGGTCGTAGGACCTAGTGGTTCGGGAAAGAGCACGGTGATCGGACTGATCATGAGATTCTACGATCCGAGCAACGGCAATCTTTGCATCGATGGGCAAGACATAAAAACCCTAAACTTACGTAGCTTGCGCAAGAAGCTAGCGCTAGTTCAACAAGAACCGGCTCTCTTTTCAACAACCATATACGAAAACATTAAGTATGGCAATGAAAACGCATCGGAGGCAGAGATCATGGAAGCTGCGAAAGCGGCGAATGCGCACGAGTTCATTATCAAGATGGAAGAAGGGTACAAAACGCACGCGGGTGATAAGGGAGTACAATTATCAGGTGGGCAGAAACAGAGAGTGGCTATCGCGAGGGCGGTTTTAAAGGACCCTTCAGTGCTTCTCCTTGATGAGGCAACAAGTGCTCTGGACACGAGCTCGGAGAAACTAGTCCAAGAGGCGCTAGACAAGCTAATGAAGGGACGAACGACGGTATTGGTGGCTCATAGGCTGTCGACCATAAGAAAAGCAGATACGGTCGCTGTGTTGCATAAGGGAAGGGTTGTAGAGAAAGGAAGTCATAGAGAGCTTGTTTCAATACCTAATGGGTTTTATAAGCAATTGACGAGTCTTCAAGAAGTACTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1245

Amino Acids

135.77

Weight (kDa)

9.16

Isoelectric Point (pI)

36.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 2 - 105 2.4e-14 ABC transporter transmembrane region
ABC_tran PF00005 176 - 324 3.6e-35 ABC transporter
SMC_N PF02463 209 - 366 4.2e-06 RecF/RecN/SMC N terminal domain
ABC_membrane PF00664 470 - 740 1.5e-50 ABC transporter transmembrane region
ABC_tran PF00005 808 - 957 1.5e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 2012, 3705
Acc16I TGCGCA 2 cut(s) 3236, 3363
AccB1I GGYRCC 1 cut(s) 2639
AccBSI CCGCTC 1 cut(s) 810
AccI GTMKAC 5 cut(s) 166, 291, 647, 1941, 3605
AccII CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
AclI AACGTT 1 cut(s) 1261
AclWI GGATC 7 cut(s) 12, 236, 332, 1437, 1894, 2406, 3164
AcoI YGGCCR 1 cut(s) 2033
AcsI RAATTY 3 cut(s) 772, 2020, 2815
AcuI CTGAAG 4 cut(s) 450, 1918, 2574, 3468
AcvI CACGTG 1 cut(s) 2600
AfaI GTAC 7 cut(s) 284, 900, 2067, 2783, 3395, 3423, 3732
AfeI AGCGCT 2 cut(s) 1615, 3249
AfiI CCNNNNNNNGG 3 cut(s) 1280, 3179, 3502
AhlI ACTAGT 1 cut(s) 3538
AleI CACNNNNGTG 1 cut(s) 3408
Alw21I GWGCWC 5 cut(s) 2080, 2392, 3140, 3517, 3530
Alw26I GTCTC 8 cut(s) 38, 662, 1820, 2433, 2745, 2780, 2804, 2945
AlwI GGATC 7 cut(s) 12, 236, 332, 1437, 1894, 2406, 3164
AlwNI CAGNNNCTG 1 cut(s) 2441
Ama87I CYCGRG 3 cut(s) 1563, 1846, 2921
Aor51HI AGCGCT 2 cut(s) 1615, 3249
AoxI GGCC 1 cut(s) 2033
ApoI RAATTY 3 cut(s) 772, 2020, 2815
Asp700I GAANNNNTTC 2 cut(s) 232, 1906
AspLEI GCGC 8 cut(s) 113, 176, 1616, 2157, 3237, 3250, 3364, 3553
AspS9I GGNCC 3 cut(s) 1217, 3116, 3478
AsuC2I CCSGG 1 cut(s) 3094
AsuHPI GGTGA 4 cut(s) 730, 2392, 3154, 3422
AsuII TTCGAA 1 cut(s) 1714
AsuNHI GCTAGC 1 cut(s) 3244
AvaI CYCGRG 3 cut(s) 1563, 1846, 2921
AvaII GGWCC 3 cut(s) 1217, 3116, 3478
AxyI CCTNAGG 1 cut(s) 970
BaeGI GKGCMC 1 cut(s) 2912
BalI TGGCCA 1 cut(s) 2035
BanI GGYRCC 1 cut(s) 2639
BanII GRGCYC 1 cut(s) 3530
BauI CACGAG 2 cut(s) 3365, 3523
BbrPI CACGTG 1 cut(s) 2600
BbsI GAAGAC 3 cut(s) 884, 1958, 3713
Bbv12I GWGCWC 5 cut(s) 2080, 2392, 3140, 3517, 3530
BccI CCATC 7 cut(s) 91, 947, 1292, 1739, 2307, 3191, 3376
BceAI ACGGC 3 cut(s) 922, 2508, 3196
BcgI CGANNNNNNTGC 4 cut(s) 2112, 2146, 3127, 3161
BciVI GTATCC 2 cut(s) 2723, 3048
BclI TGATCA 3 cut(s) 2148, 2796, 3153
BcnI CCSGG 1 cut(s) 3094
BcoDI GTCTC 8 cut(s) 38, 662, 1820, 2433, 2745, 2780, 2804, 2945
BcuI ACTAGT 1 cut(s) 3538
BfmI CTRYAG 3 cut(s) 237, 636, 2464
BfoI RGCGCY 4 cut(s) 177, 1617, 3251, 3554
BfuI GTATCC 2 cut(s) 2723, 3048
BglII AGATCT 1 cut(s) 2652
BlpI GCTNAGC 1 cut(s) 2678
BmcAI AGTACT 2 cut(s) 2067, 3732
Bme1390I CCNGG 1 cut(s) 3094
Bme18I GGWCC 3 cut(s) 1217, 3116, 3478
BmeT110I CYCGRG 3 cut(s) 1563, 1846, 2921
BmgT120I GGNCC 3 cut(s) 1217, 3116, 3478
BmiI GGNNCC 4 cut(s) 603, 2053, 2641, 3480
BmrFI CCNGG 1 cut(s) 3094
BmsI GCATC 8 cut(s) 333, 460, 478, 673, 1612, 2359, 3201, 3329
BmtI GCTAGC 1 cut(s) 3248
BoxI GACNNNNGTC 2 cut(s) 1811, 2414
BpiI GAAGAC 3 cut(s) 884, 1958, 3713
BplI GAGNNNNNCTC 2 cut(s) 2374, 2406
BpmI CTGGAG 4 cut(s) 2010, 2094, 2208, 2949
Bpu10I CCTNAGC 1 cut(s) 1005
Bpu1102I GCTNAGC 1 cut(s) 2678
Bpu14I TTCGAA 1 cut(s) 1714
BpuEI CTTGAG 6 cut(s) 764, 1759, 1821, 1863, 1900, 2965
BpuMI CCSGG 1 cut(s) 3094
Bsa29I ATCGAT 2 cut(s) 395, 3195
BsaAI YACGTR 3 cut(s) 2600, 2785, 3227
BsaBI GATNNNNATC 1 cut(s) 444
BsaI GGTCTC 2 cut(s) 2780, 2945
BsaJI CCNNGG 1 cut(s) 904
BsaWI WCCGGW 1 cut(s) 1763
Bsc4I CCNNNNNNNGG 3 cut(s) 1280, 3179, 3502
Bse118I RCCGGY 1 cut(s) 3265
Bse1I ACTGG 1 cut(s) 2344
Bse21I CCTNAGG 1 cut(s) 970
Bse3DI GCAATG 3 cut(s) 1091, 2511, 3316
Bse8I GATNNNNATC 1 cut(s) 444
BseCI ATCGAT 2 cut(s) 395, 3195
BseDI CCNNGG 1 cut(s) 904
BseGI GGATG 5 cut(s) 33, 348, 939, 2318, 2332
BseJI GATNNNNATC 1 cut(s) 444
BseLI CCNNNNNNNGG 3 cut(s) 1280, 3179, 3502
BseMI GCAATG 3 cut(s) 1091, 2511, 3316
BseMII CTCAG 9 cut(s) 545, 669, 1293, 1517, 1545, 1788, 2301, 2756, 2880
BseNI ACTGG 1 cut(s) 2344
BseRI GAGGAG 6 cut(s) 74, 639, 1806, 1809, 2398, 2571
BseSI GKGCMC 1 cut(s) 2912
BseYI CCCAGC 1 cut(s) 1106
BsgI GTGCAG 2 cut(s) 2082, 2190
Bsh1236I CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
Bsh1285I CGRYCG 2 cut(s) 3111, 3630
BshFI GGCC 1 cut(s) 2035
BshNI GGYRCC 1 cut(s) 2639
BshVI ATCGAT 2 cut(s) 395, 3195
BsiEI CGRYCG 2 cut(s) 3111, 3630
BsiHKAI GWGCWC 5 cut(s) 2080, 2392, 3140, 3517, 3530
BsiHKCI CYCGRG 3 cut(s) 1563, 1846, 2921
BsiSI CCGG 3 cut(s) 1764, 3093, 3266
BslFI GGGAC 1 cut(s) 3585
BslI CCNNNNNNNGG 3 cut(s) 1280, 3179, 3502
BsmAI GTCTC 8 cut(s) 38, 662, 1820, 2433, 2745, 2780, 2804, 2945
BsmBI CGTCTC 2 cut(s) 38, 2804
BsmFI GGGAC 1 cut(s) 3585
BsmI GAATGC 4 cut(s) 280, 1423, 2701, 3364
BsnI GGCC 1 cut(s) 2035
Bso31I GGTCTC 2 cut(s) 2780, 2945
BsoBI CYCGRG 3 cut(s) 1563, 1846, 2921
Bsp119I TTCGAA 1 cut(s) 1714
Bsp1286I GDGCHC 6 cut(s) 2080, 2392, 2912, 3140, 3517, 3530
Bsp1720I GCTNAGC 1 cut(s) 2678
Bsp68I TCGCGA 1 cut(s) 3461
BspANI GGCC 1 cut(s) 2035
BspCNI CTCAG 9 cut(s) 544, 668, 1292, 1516, 1544, 1787, 2300, 2755, 2879
BspDI ATCGAT 2 cut(s) 395, 3195
BspFNI CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
BspHI TCATGA 1 cut(s) 3156
BspLI GGNNCC 4 cut(s) 603, 2053, 2641, 3480
BspOI GCTAGC 1 cut(s) 3248
BspPI GGATC 7 cut(s) 12, 236, 332, 1437, 1894, 2406, 3164
BspQI GCTCTTC 1 cut(s) 1993
BspT104I TTCGAA 1 cut(s) 1714
BspT107I GGYRCC 1 cut(s) 2639
BspTNI GGTCTC 2 cut(s) 2780, 2945
BsrBI CCGCTC 1 cut(s) 810
BsrDI GCAATG 3 cut(s) 1091, 2511, 3316
BsrFI RCCGGY 1 cut(s) 3265
BsrI ACTGG 1 cut(s) 2344
BssAI RCCGGY 1 cut(s) 3265
BssECI CCNNGG 1 cut(s) 904
BssNAI GTATAC 1 cut(s) 167
BssSI CACGAG 2 cut(s) 3365, 3523
Bst1107I GTATAC 1 cut(s) 167
Bst2BI CACGAG 2 cut(s) 3365, 3523
Bst6I CTCTTC 7 cut(s) 194, 656, 696, 741, 1897, 1993, 2511
BstAPI GCANNNNNTGC 5 cut(s) 1460, 2069, 2639, 3189, 3512
BstBAI YACGTR 3 cut(s) 2600, 2785, 3227
BstBI TTCGAA 1 cut(s) 1714
BstC8I GCNNGC 8 cut(s) 1650, 2684, 2883, 2912, 2916, 3233, 3246, 3405
BstDSI CCRYGG 1 cut(s) 904
BstENI CCTNNNNNAGG 2 cut(s) 1278, 3500
BstF5I GGATG 5 cut(s) 33, 348, 939, 2318, 2332
BstFNI CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
BstH2I RGCGCY 4 cut(s) 177, 1617, 3251, 3554
BstHHI GCGC 8 cut(s) 113, 176, 1616, 2157, 3237, 3250, 3364, 3553
BstMAI GTCTC 8 cut(s) 38, 662, 1820, 2433, 2745, 2780, 2804, 2945
BstMCI CGRYCG 2 cut(s) 3111, 3630
BstPAI GACNNNNGTC 2 cut(s) 1811, 2414
BstSCI CCNGG 1 cut(s) 3092
BstSFI CTRYAG 3 cut(s) 237, 636, 2464
BstSLI GKGCMC 1 cut(s) 2912
BstSNI TACGTA 2 cut(s) 2785, 3227
BstUI CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
BstV2I GAAGAC 3 cut(s) 884, 1958, 3713
BstX2I RGATCY 4 cut(s) 17, 241, 1886, 2652
BstYI RGATCY 4 cut(s) 17, 241, 1886, 2652
BstZ17I GTATAC 1 cut(s) 167
Bsu15I ATCGAT 2 cut(s) 395, 3195
Bsu36I CCTNAGG 1 cut(s) 970
BsuI GTATCC 2 cut(s) 2723, 3048
BsuRI GGCC 1 cut(s) 2035
BsuTUI ATCGAT 2 cut(s) 395, 3195
BtgI CCRYGG 1 cut(s) 904
BtgZI GCGATG 1 cut(s) 483
BtsCI GGATG 5 cut(s) 33, 348, 939, 2318, 2332
BtsI GCAGTG 1 cut(s) 2439
BtsIMutI CAGTG 8 cut(s) 93, 605, 732, 964, 2337, 2439, 2598, 3492
BtuMI TCGCGA 1 cut(s) 3461
Cac8I GCNNGC 8 cut(s) 1650, 2684, 2883, 2912, 2916, 3233, 3246, 3405
CaiI CAGNNNCTG 1 cut(s) 2441
CciI TCATGA 1 cut(s) 3156
CfoI GCGC 8 cut(s) 113, 176, 1616, 2157, 3237, 3250, 3364, 3553
Cfr10I RCCGGY 1 cut(s) 3265
Cfr13I GGNCC 3 cut(s) 1217, 3116, 3478
ClaI ATCGAT 2 cut(s) 395, 3195
CseI GACGC 1 cut(s) 2243
Csp6I GTAC 7 cut(s) 283, 899, 2066, 2782, 3394, 3422, 3731
CspCI CAANNNNNGTGG 2 cut(s) 2574, 2609
CviQI GTAC 7 cut(s) 283, 899, 2066, 2782, 3394, 3422, 3731
DraI TTTAAA 2 cut(s) 2544, 3474
EaeI YGGCCR 1 cut(s) 2033
Eam1104I CTCTTC 7 cut(s) 194, 656, 696, 741, 1897, 1993, 2511
EarI CTCTTC 7 cut(s) 194, 656, 696, 741, 1897, 1993, 2511
Ecl136II GAGCTC 1 cut(s) 3528
Eco105I TACGTA 2 cut(s) 2785, 3227
Eco24I GRGCYC 1 cut(s) 3530
Eco31I GGTCTC 2 cut(s) 2780, 2945
Eco32I GATATC 2 cut(s) 2899, 2961
Eco47I GGWCC 3 cut(s) 1217, 3116, 3478
Eco47III AGCGCT 2 cut(s) 1615, 3249
Eco53kI GAGCTC 1 cut(s) 3528
Eco57I CTGAAG 4 cut(s) 450, 1918, 2574, 3468
Eco72I CACGTG 1 cut(s) 2600
Eco81I CCTNAGG 1 cut(s) 970
Eco88I CYCGRG 3 cut(s) 1563, 1846, 2921
EcoICRI GAGCTC 1 cut(s) 3528
EcoNI CCTNNNNNAGG 2 cut(s) 1278, 3500
EcoO109I RGGNCCY 2 cut(s) 3116, 3478
EcoRI GAATTC 1 cut(s) 2020
EcoRV GATATC 2 cut(s) 2899, 2961
EcoT22I ATGCAT 1 cut(s) 2044
EcoT38I GRGCYC 1 cut(s) 3530
Esp3I CGTCTC 2 cut(s) 38, 2804
FalI AAGNNNNNCTT 4 cut(s) 1909, 1941, 2684, 2716
FaqI GGGAC 1 cut(s) 3585
FauI CCCGC 3 cut(s) 269, 535, 3400
FauNDI CATATG 2 cut(s) 2038, 2635
FbaI TGATCA 3 cut(s) 2148, 2796, 3153
FblI GTMKAC 5 cut(s) 166, 291, 647, 1941, 3605
FokI GGATG 5 cut(s) 20, 355, 926, 2325, 2339
FriOI GRGCYC 1 cut(s) 3530
FspAI RTGCGCAY 1 cut(s) 3363
FspI TGCGCA 2 cut(s) 3236, 3363
GlaI GCGC 8 cut(s) 112, 175, 1615, 2156, 3236, 3249, 3363, 3552
GsaI CCCAGC 1 cut(s) 1110
GsuI CTGGAG 4 cut(s) 2010, 2094, 2208, 2949
HaeII RGCGCY 4 cut(s) 177, 1617, 3251, 3554
HaeIII GGCC 1 cut(s) 2035
HapII CCGG 3 cut(s) 1764, 3093, 3266
HgaI GACGC 1 cut(s) 2243
HhaI GCGC 8 cut(s) 113, 176, 1616, 2157, 3237, 3250, 3364, 3553
Hin6I GCGC 8 cut(s) 111, 174, 1614, 2155, 3235, 3248, 3362, 3551
HinP1I GCGC 8 cut(s) 111, 174, 1614, 2155, 3235, 3248, 3362, 3551
HincII GTYRAC 3 cut(s) 1723, 2118, 3606
HindII GTYRAC 3 cut(s) 1723, 2118, 3606
HindIII AAGCTT 2 cut(s) 1322, 1930
HpaI GTTAAC 1 cut(s) 2118
HpaII CCGG 3 cut(s) 1764, 3093, 3266
HphI GGTGA 4 cut(s) 730, 2392, 3154, 3422
Hpy166II GTNNAC 8 cut(s) 167, 292, 648, 1723, 1942, 2118, 2667, 3606
Hpy8I GTNNAC 8 cut(s) 167, 292, 648, 1723, 1942, 2118, 2667, 3606
Hpy99I CGWCG 3 cut(s) 1691, 2162, 3583
HpyCH4IV ACGT 7 cut(s) 590, 1261, 2160, 2599, 2784, 3024, 3226
HpySE526I ACGT 7 cut(s) 590, 1261, 2160, 2599, 2784, 3024, 3226
HspAI GCGC 8 cut(s) 111, 174, 1614, 2155, 3235, 3248, 3362, 3551
Ksp22I TGATCA 3 cut(s) 2148, 2796, 3153
KspAI GTTAAC 1 cut(s) 2118
LguI GCTCTTC 1 cut(s) 1993
LmnI GCTCC 4 cut(s) 807, 2057, 2075, 2189
LweI GCATC 8 cut(s) 333, 460, 478, 673, 1612, 2359, 3201, 3329
MaeII ACGT 7 cut(s) 590, 1261, 2160, 2599, 2784, 3024, 3226
MaeIII GTNAC 6 cut(s) 711, 1493, 2200, 2437, 2488, 2854
MbiI CCGCTC 1 cut(s) 810
MfeI CAATTG 4 cut(s) 492, 1557, 1816, 3710
MflI RGATCY 4 cut(s) 17, 241, 1886, 2652
MhlI GDGCHC 6 cut(s) 2080, 2392, 2912, 3140, 3517, 3530
MlsI TGGCCA 1 cut(s) 2035
MluNI TGGCCA 1 cut(s) 2035
MlyI GAGTC 3 cut(s) 1366, 1801, 3727
MmeI TCCRAC 4 cut(s) 320, 814, 1489, 2248
Mox20I TGGCCA 1 cut(s) 2035
Mph1103I ATGCAT 1 cut(s) 2044
MroXI GAANNNNTTC 2 cut(s) 232, 1906
MscI TGGCCA 1 cut(s) 2035
MseI TTAA 8 cut(s) 839, 1326, 1476, 1787, 2117, 2543, 3300, 3473
MslI CAYNNNNRTG 2 cut(s) 1250, 3408
Msp20I TGGCCA 1 cut(s) 2035
MspA1I CMGCKG 1 cut(s) 2483
MspI CCGG 3 cut(s) 1764, 3093, 3266
MspR9I CCNGG 1 cut(s) 3094
MunI CAATTG 4 cut(s) 492, 1557, 1816, 3710
Mva1269I GAATGC 4 cut(s) 280, 1423, 2701, 3364
MvnI CGCG 5 cut(s) 264, 998, 2157, 3407, 3461
NciI CCSGG 1 cut(s) 3094
NdeI CATATG 2 cut(s) 2038, 2635
NheI GCTAGC 1 cut(s) 3244
NlaIV GGNNCC 4 cut(s) 603, 2053, 2641, 3480
NmeAIII GCCGAG 1 cut(s) 67
NmuCI GTSAC 3 cut(s) 711, 2437, 2854
NruI TCGCGA 1 cut(s) 3461
NsbI TGCGCA 2 cut(s) 3236, 3363
NsiI ATGCAT 1 cut(s) 2044
NspV TTCGAA 1 cut(s) 1714
OliI CACNNNNGTG 1 cut(s) 3408
PaeR7I CTCGAG 2 cut(s) 1563, 1846
PagI TCATGA 1 cut(s) 3156
PciSI GCTCTTC 1 cut(s) 1993
PcsI WCGNNNNNNNCGW 1 cut(s) 587
PctI GAATGC 4 cut(s) 280, 1423, 2701, 3364
PdmI GAANNNNTTC 2 cut(s) 232, 1906
PflFI GACNNNGTC 1 cut(s) 1661
PleI GAGTC 3 cut(s) 1365, 1801, 3726
PmaCI CACGTG 1 cut(s) 2600
PmlI CACGTG 1 cut(s) 2600
PpsI GAGTC 3 cut(s) 1365, 1801, 3726
Ppu21I YACGTR 3 cut(s) 2600, 2785, 3227
PpuMI RGGWCCY 2 cut(s) 3116, 3478
PshAI GACNNNNGTC 2 cut(s) 1811, 2414
PsiI TTATAA 2 cut(s) 2012, 3705
Psp124BI GAGCTC 1 cut(s) 3530
Psp1406I AACGTT 1 cut(s) 1261
Psp5II RGGWCCY 2 cut(s) 3116, 3478
PspCI CACGTG 1 cut(s) 2600
PspFI CCCAGC 1 cut(s) 1106
PspN4I GGNNCC 4 cut(s) 603, 2053, 2641, 3480
PspPI GGNCC 3 cut(s) 1217, 3116, 3478
PspPPI RGGWCCY 2 cut(s) 3116, 3478
PspXI VCTCGAGB 1 cut(s) 1563
PstNI CAGNNNCTG 1 cut(s) 2441
PsuI RGATCY 4 cut(s) 17, 241, 1886, 2652
PsyI GACNNNGTC 1 cut(s) 1661
PvuII CAGCTG 1 cut(s) 2483
RruI TCGCGA 1 cut(s) 3461
RsaI GTAC 7 cut(s) 284, 900, 2067, 2783, 3395, 3423, 3732
RsaNI GTAC 7 cut(s) 283, 899, 2066, 2782, 3394, 3422, 3731
RseI CAYNNNNRTG 2 cut(s) 1250, 3408
SacI GAGCTC 1 cut(s) 3530
SalI GTCGAC 1 cut(s) 3604
SapI GCTCTTC 1 cut(s) 1993
SaqAI TTAA 8 cut(s) 839, 1326, 1476, 1787, 2117, 2543, 3300, 3473
Sau96I GGNCC 3 cut(s) 1217, 3116, 3478
ScaI AGTACT 2 cut(s) 2067, 3732
SchI GAGTC 3 cut(s) 1366, 1801, 3727
ScrFI CCNGG 1 cut(s) 3094
SduI GDGCHC 6 cut(s) 2080, 2392, 2912, 3140, 3517, 3530
SfaNI GCATC 8 cut(s) 333, 460, 478, 673, 1612, 2359, 3201, 3329
SfcI CTRYAG 3 cut(s) 237, 636, 2464
Sfr274I CTCGAG 2 cut(s) 1563, 1846
SfuI TTCGAA 1 cut(s) 1714
SinI GGWCC 3 cut(s) 1217, 3116, 3478
SlaI CTCGAG 2 cut(s) 1563, 1846
SmiMI CAYNNNNRTG 2 cut(s) 1250, 3408
SmlI CTYRAG 8 cut(s) 779, 1563, 1738, 1836, 1846, 1878, 1915, 2980
SmoI CTYRAG 8 cut(s) 779, 1563, 1738, 1836, 1846, 1878, 1915, 2980
SnaBI TACGTA 2 cut(s) 2785, 3227
SpeI ACTAGT 1 cut(s) 3538
SspI AATATT 1 cut(s) 1399
SstI GAGCTC 1 cut(s) 3530
StyD4I CCNGG 1 cut(s) 3092
TaiI ACGT 7 cut(s) 593, 1264, 2163, 2602, 2787, 3027, 3229
TaqI TCGA 8 cut(s) 395, 1564, 1714, 1847, 1994, 2408, 3195, 3605
TaqII GACCGA 2 cut(s) 521, 1205
TatI WGTACW 4 cut(s) 282, 2065, 3421, 3730
TauI GCSGC 3 cut(s) 983, 2519, 3356
Tru1I TTAA 8 cut(s) 839, 1326, 1476, 1787, 2117, 2543, 3300, 3473
Tru9I TTAA 8 cut(s) 839, 1326, 1476, 1787, 2117, 2543, 3300, 3473
TscAI CASTG 8 cut(s) 93, 612, 732, 964, 2344, 2446, 2598, 3492
TseFI GTSAC 3 cut(s) 711, 2437, 2854
Tsp45I GTSAC 3 cut(s) 711, 2437, 2854
TspDTI ATGAA 7 cut(s) 1284, 1462, 1617, 2830, 3327, 3361, 3581
TspGWI ACGGA 1 cut(s) 62
TspRI CASTG 8 cut(s) 93, 612, 732, 964, 2344, 2446, 2598, 3492
Tth111I GACNNNGTC 1 cut(s) 1661
VpaK11BI GGWCC 3 cut(s) 1217, 3116, 3478
XagI CCTNNNNNAGG 2 cut(s) 1278, 3500
XapI RAATTY 3 cut(s) 772, 2020, 2815
XbaI TCTAGA 1 cut(s) 1148
XhoI CTCGAG 2 cut(s) 1563, 1846
XmiI GTMKAC 5 cut(s) 166, 291, 647, 1941, 3605
XmnI GAANNNNTTC 2 cut(s) 232, 1906
ZrmI AGTACT 2 cut(s) 2067, 3732
Zsp2I ATGCAT 1 cut(s) 2044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.