MD08G1010400.v1.1

ABC transporter B family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0001951
Physical Location & Seq
Reverse (-)
763311 .. 767954
4644 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1010400.v1.1.491

Sequence Viewer

Length: 3741 bp
ATGGAGGAAGTAGAGCTTTCCTCCGACAAGTTTTCGGACGGAAACCCGCTTCGGAAAATGGACAAACAAACGAACCCGAGCAAGCAGCGCAGCGTGTCGCTCATCGGACTTTTCGCTGCTGCCGATAAGGCGGACTATTTGTTAATGTTTTTGGGAAGTGTTGGGGCTTGTATCCATGGTGGTGTGCTTCCTGTGTTCTTTGTTGTTTTTGGTCGTATGATCGATTCGTTAGGGCATCTGGCAAAGAATCCGCAGCAACAGTCTGCCCGGGTTTCCGAGTATGCTTTGTACTTGGTATATCTTGGACTCGTTCTCTTCGCATCAGCATGGATTGGTGTAGCATTCTGGACACGAACGGGGGAGAGGCAGACAGCGCGTTTGCGAATGAAGTACCTCCAGTCAGTTCTAAACAAGGACATAAATTTTTTCGACACTGAAGCCAGAGACACTAACATTATTTTCCACATTTCAAGTGATGCAATTCTAGTGCAAGATGCAATAGGTGACAAGACCGGCCATGCGTTGCGCTACCTTTCGCAGTTCCTTATCGGATTTGCCATCGGGTTTACATCAGTATGGCAGCTTACGCTTCTGACGTTGGCTGTGGTTCCGTTCATAGCTATTGCAGGTGGAGCTTATACTATAATCATGTCTACCTTATCCGAAAAGGGTGAGGCTGCTTATGCAGAAGCCGGCAAGGTTGCTGAAGAGGTTATTTCACAGATTCGTACTGTGTACTCATTCGGGGGAGAGGACAGAGCCATTGAAGCATACTCAACCTCACTGAACAAGGCCCTCAAGCTGGGAAAGAAGAGCGGTGTTGCGAAGGGAGTTGGCGTAGGGTTTACATATGGGCTGTTGTTTTGCGCTTGGGCATTGCTTCTGTGGTACGCCGGCATACTTGTAAGGCATCACGACACGAACGGAGGGAAAGCATTCACAACTATCCTCAATGTCATCTTCAGTGGATTTGCTCTCGGCCAAGCTGCTCCAAACCTTGCTGCAATTGCAAAGGGTAGAGCTGCTGCTGGTAATATCATGACGATGATTGATTCGGATTCCAATTCCTCTAAGGTATCGGACAATGGAATAGTGTTGCCGAAAGTATCTGGGCAGATTGACTTCTGTGAGGTTGGTTTCGCCTATTCTTCACGACCAAACATGGTGTTTGAGAATTTGAGCTTTTCGATTGGAGCTGGCAAGACATTTGCAGTTGTTGGTCCTAGTGGTTCAGGAAAGAGCACTGTTATATCCATGATTCAGCGATTCTACAACCCCGTTTCAGGTAAAATACTGTTGGATGGACATGAACTCGGAACTCTCCGACTGAAATGGTTACGAGAACAGATGGGATTGGTAAGTCAAGAACCGGCATTGTTTGCGACTACTATAGCCGGCAACATTTTGTACGGTAAAGAAGACGCAGACATGGATCAGATCATGGAAGCTGCTAAAGCTGCAAATGCACATTCTTTCATTGAAGGATTGCCTGATGGCTACAACACTCAGGCAGGAGAAGGAGGAACTCAGCTTTCCGGAGGGCAAAAGCAGCGCATAGCTATTGCAAGAGCAGTGCTGCGAAACCCAAAAATACTACTTTTAGATGAGGCCACAAGCGCTCTTGATGCAGAATCAGAACTAATCGTTCAGCAGGCACTCGAGAAAATCATGTTGCATCGAACTACAGTCATCGTTGCTCATCGGTTGTCCACTATCCGGGATGTGGACACAATCGTTGTCTTGAAGAACGGTCAGGTTGCTGAGAGTGGCAACCATTCAGACTTGATGTCCAAGAAAGGGGAGTATGCAAATCTGGTGAGCTTGCAGGTACTGGAACATGTTAAAGATTCTGGCTTAGTATCTGGACATTCTTCGAGAGAATCCAGCTTTCGCGATACCACGAGTAACTATCCGCAGGAGGACAAGGCAACCACAACCAAACAGCAGAAGCCAAGTGATCAAAACAGCTCTGCTCCTACTGCATCAGTGTGGGAATTAGTGAAACTAAATGCGCCCGAGTGGCCTTATGCAATACTCGGGTCACTTGGTGCAGTTCTGGCCGGCATGGAAGCTCCTCTTTTCGCCCTTGGAATAACTCACGTCTTGACAGCGTTTTACGCTCCTAGTGGCTCTCAAATCAAACATGATGTTGATAGGATAGCTCTCATATTTGTTGGCTTGGCTGCTCTTACTGTCCCAATATATCTCCTGCAGCACTACTTTTATACATTGATGGGGGAGCGGCTCACAACTCGCGTTCGCTTATTAATGTTCAAAGCTATCCTTTCAAACGAAGTTGGTTGGTTTGATTTGGATGAGAATAACACCGGAGCACTGACATCAACTTTAGCAGCAAATGCAACATTAGTGCGAAGCGCTCTAGCTGACCGTCTATCAACAATCGTGCAGAATCTCGCACTCGCAGTGACTGCTTTCGCTATTGCTTTCACCTTAAGTTGGCGTATTGCAGCTGTCGTGAGTGCCTCCCTCCCTCTACTTATCGGAGCTTCCATAACTGAGCAACTGTTTCTGAAGGGATTCGGAGGGGACTACAACCATGCCTACTCAAAAGCAACTTCCGTGGCACGCGAAGCGATTGCCAACATACGTACTGTTGCTGCATTTGGTTGCGAAGAACGGATATCGCTCCAGTTTTCCTTGGAACTAAACAAGCCAAACAAGCAAGCAGTTCTAAGAGGCCACATATCAGGTTTCTGCTACGGTTTATCGCAGTTTTTCGCGTTCTGTTCTTATGCGCTTGGCCTTTGGTATGCATCAGTACTAATCAAGGATAAAGACTCGAATTTCGGAGACATCATCAAGTCCTTCATGGTCTTAATAGTCACGTCACTGAGTATTGCAGAAACCCTAGCTCTCACACCAGAAATTATGAAGGGATCGCAAGTGCTCGGCCCAATTTTCAGTATCCTAAAGCGAGAAACGGCCATACATCGTAATGATCCCAGATCAAATGTTGTTGCTAATGTCAAAGGAGATATAGAGTTCAGGAATGTGAGTTTCTGGTACCCTGCAAGGCCGGATATCACCATTTTCGACCATTTGAATTTACGGGTTTCAGCAGGAAAGAGCCTTGCAGTAGTCGGGCCAAGTGGTTCTGGGAAGAGTACGGTGATTGCACTGGTAATGAGATTTTATGACCCCGTTTCTGGTACGGTTCTAATTGATGGATACGATATCAGAAGTATGAACTTGAAATCGTTGAGGAAGAGAATAAGTCTAGTTCAGCAAGAGCCGGCATTGTTCTGCACAACAATTTATGAGAACATAAAGTATGGGCACGAGGAAGCATCGGAAGTAGAGGTAGTGACAGCGGCGAAAGCAGCGAATGCACACGGTTTCATAAGTCGAATGCCTGAAGGGTACAAAACGCAAGTGGGAGAGAAAGGGGTGCAGTTATCGGGAGGGCAGAAACAGAGGGTGGCAATTGCCAGAGCAATACTGAAAGATCCGTCGATTCTTCTTCTGGACGAAGCAACAAGTGCATTGGACACAGAGTCTGAGAAGCTGGTCCAAGAGGCTCTTGATAAGCTAATGGAGGGCCGAACGACGATCTTGGTAGCACACCGGTTGTCAACTATTCGTGATGCCAACCGTATCGCCCTGCTACAAAATGGTAGGGTGGCTGAAATGGGAAGCCACGAGCAGCTGATTGGAAGACCTGGTAGTCTCTACAAACAATTAGTTAGTCTACAGCAACAAAAGACACAAGCGACTGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko02010 ko04976 ko05206 ko05226 map02010 map04976 map05206 map05226
Pfam Domains
Protein Families
ABC_membrane ABC_tran

Protein Analysis

1247

Amino Acids

135.09

Weight (kDa)

8.31

Isoelectric Point (pI)

33.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ABC_membrane PF00664 50 - 321 2.8e-54 ABC transporter transmembrane region
ABC_tran PF00005 391 - 539 2.6e-33 ABC transporter
ABC_membrane PF00664 680 - 950 4.6e-51 ABC transporter transmembrane region
ABC_tran PF00005 1019 - 1167 7e-33 ABC transporter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 617
AasI GACNNNNNNGTC 1 cut(s) 3684
Acc36I ACCTGC 2 cut(s) 617, 1816
Acc65I GGTACC 1 cut(s) 3024
AccB1I GGYRCC 1 cut(s) 3024
AccBSI CCGCTC 2 cut(s) 816, 2242
AccI GTMKAC 2 cut(s) 653, 3709
AccII CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
AccIII TCCGGA 1 cut(s) 1535
AciI CCGC 7 cut(s) 47, 131, 251, 816, 1913, 2242, 3332
AclWI GGATC 4 cut(s) 1440, 2905, 2954, 3461
AcoI YGGCCR 4 cut(s) 514, 979, 2058, 2943
AcsI RAATTY 4 cut(s) 421, 1174, 2803, 3064
AcuI CTGAAG 5 cut(s) 456, 726, 946, 2552, 3396
AdeI CACNNNGTG 1 cut(s) 2048
AfeI AGCGCT 2 cut(s) 1618, 2377
AfiI CCNNNNNNNGG 7 cut(s) 802, 1283, 1716, 1723, 1797, 2457, 3167
AflII CTTAAG 1 cut(s) 2452
AflIII ACRYGT 1 cut(s) 1837
AgeI ACCGGT 1 cut(s) 3585
AgsI TTSAA 8 cut(s) 471, 767, 1481, 1744, 2275, 2289, 3064, 3214
AhdI GACNNNNNGTC 2 cut(s) 1786, 3514
AjiI CACGTC 2 cut(s) 2101, 2847
AjnI CCWGG 1 cut(s) 3679
Alw21I GWGCWC 3 cut(s) 1244, 2335, 2910
Alw26I GTCTC 3 cut(s) 438, 2805, 3692
AlwI GGATC 4 cut(s) 1440, 2905, 2954, 3461
AlwNI CAGNNNCTG 3 cut(s) 1831, 2429, 3518
Ama87I CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
Aor13HI TCCGGA 1 cut(s) 1535
Aor51HI AGCGCT 2 cut(s) 1618, 2377
ApoI RAATTY 4 cut(s) 421, 1174, 2803, 3064
ArsI GACNNNNNNTTYG 4 cut(s) 361, 393, 2789, 2821
AseI ATTAAT 1 cut(s) 2267
AsiGI ACCGGT 1 cut(s) 3585
Asp700I GAANNNNTTC 2 cut(s) 935, 2537
Asp718I GGTACC 1 cut(s) 3024
AspLEI GCGC 9 cut(s) 90, 376, 528, 869, 1554, 1619, 2014, 2378, 2758
AspS9I GGNCC 6 cut(s) 793, 1220, 2912, 3104, 3529, 3559
AsuC2I CCSGG 3 cut(s) 268, 269, 1718
AsuHPI GGTGA 6 cut(s) 515, 683, 1828, 2440, 3037, 3142
AvaI CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
AvaII GGWCC 2 cut(s) 1220, 3529
BaeGI GKGCMC 1 cut(s) 3300
BanI GGYRCC 1 cut(s) 3024
BauI CACGAG 3 cut(s) 1900, 3299, 3659
BbsI GAAGAC 2 cut(s) 1425, 3682
Bbv12I GWGCWC 3 cut(s) 1244, 2335, 2910
BccI CCATC 6 cut(s) 566, 1295, 1342, 1487, 2227, 3179
BceAI ACGGC 1 cut(s) 2958
BcgI CGANNNNNNTGC 2 cut(s) 2579, 2613
BciT130I CCWGG 1 cut(s) 3681
BciVI GTATCC 3 cut(s) 182, 2936, 3182
BclI TGATCA 1 cut(s) 1957
BcnI CCSGG 3 cut(s) 268, 269, 1718
BcoDI GTCTC 3 cut(s) 438, 2805, 3692
BfaI CTAG 6 cut(s) 485, 1224, 2124, 2381, 2870, 3239
BfmI CTRYAG 4 cut(s) 1389, 1683, 2210, 3710
BfoI RGCGCY 2 cut(s) 1620, 2379
BfrI CTTAAG 1 cut(s) 2452
BfuAI ACCTGC 2 cut(s) 617, 1816
BfuI GTATCC 3 cut(s) 182, 2936, 3182
BglI GCCNNNNNGGC 2 cut(s) 128, 2020
BmcAI AGTACT 1 cut(s) 2781
Bme1390I CCNGG 4 cut(s) 268, 269, 1718, 3681
Bme18I GGWCC 2 cut(s) 1220, 3529
BmeRI GACNNNNNGTC 2 cut(s) 1786, 3514
BmeT110I CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
BmgBI CACGTC 2 cut(s) 2101, 2847
BmgT120I GGNCC 6 cut(s) 793, 1220, 2912, 3104, 3529, 3559
BmiI GGNNCC 2 cut(s) 609, 3026
BmrFI CCNGG 4 cut(s) 268, 269, 1718, 3681
BpiI GAAGAC 2 cut(s) 1425, 3682
BplI GAGNNNNNCTC 1 cut(s) 37
BpmI CTGGAG 2 cut(s) 380, 2633
BpuEI CTTGAG 1 cut(s) 782
BpuMI CCSGG 3 cut(s) 268, 269, 1718
Bsa29I ATCGAT 1 cut(s) 222
BsaAI YACGTR 1 cut(s) 2609
BsaJI CCNNGG 5 cut(s) 175, 267, 2086, 2580, 2658
BsaWI WCCGGW 3 cut(s) 1535, 2327, 3585
BsaXI ACNNNNNCTCC 2 cut(s) 822, 852
Bsc4I CCNNNNNNNGG 7 cut(s) 802, 1283, 1716, 1723, 1797, 2457, 3167
Bse118I RCCGGY 8 cut(s) 512, 692, 893, 1369, 1394, 2060, 3253, 3585
Bse1I ACTGG 4 cut(s) 397, 1836, 2650, 3144
Bse3DI GCAATG 1 cut(s) 875
BseAI TCCGGA 1 cut(s) 1535
BseBI CCWGG 1 cut(s) 3681
BseCI ATCGAT 1 cut(s) 222
BseDI CCNNGG 5 cut(s) 175, 267, 2086, 2580, 2658
BseGI GGATG 3 cut(s) 1306, 1726, 2320
BseLI CCNNNNNNNGG 7 cut(s) 802, 1283, 1716, 1723, 1797, 2457, 3167
BseMI GCAATG 1 cut(s) 875
BseMII CTCAG 6 cut(s) 1520, 1541, 1752, 2508, 2843, 3510
BseNI ACTGG 4 cut(s) 397, 1836, 2650, 3144
BseRI GAGGAG 1 cut(s) 2064
BseSI GKGCMC 1 cut(s) 3300
BseYI CCCAGC 1 cut(s) 802
BsgI GTGCAG 4 cut(s) 2070, 2426, 3250, 3431
Bsh1236I CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
BshNI GGYRCC 1 cut(s) 3024
BshTI ACCGGT 1 cut(s) 3585
BshVI ATCGAT 1 cut(s) 222
BsiHKAI GWGCWC 3 cut(s) 1244, 2335, 2910
BsiHKCI CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
BslFI GGGAC 2 cut(s) 2180, 2561
BslI CCNNNNNNNGG 7 cut(s) 802, 1283, 1716, 1723, 1797, 2457, 3167
BsmAI GTCTC 3 cut(s) 438, 2805, 3692
BsmFI GGGAC 2 cut(s) 2180, 2561
BsmI GAATGC 4 cut(s) 341, 935, 3352, 3375
BsoBI CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
Bsp1286I GDGCHC 4 cut(s) 1244, 2335, 2910, 3300
Bsp13I TCCGGA 1 cut(s) 1535
Bsp143I GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
Bsp19I CCATGG 1 cut(s) 175
Bsp68I TCGCGA 1 cut(s) 1893
BspACI CCGC 7 cut(s) 47, 131, 251, 816, 1913, 2242, 3332
BspCNI CTCAG 6 cut(s) 1519, 1540, 1753, 2509, 2844, 3511
BspDI ATCGAT 1 cut(s) 222
BspEI TCCGGA 1 cut(s) 1535
BspFNI CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
BspHI TCATGA 1 cut(s) 1038
BspLI GGNNCC 2 cut(s) 609, 3026
BspMAI CTGCAG 1 cut(s) 2214
BspMI ACCTGC 2 cut(s) 617, 1816
BspPI GGATC 4 cut(s) 1440, 2905, 2954, 3461
BspQI GCTCTTC 1 cut(s) 806
BspT107I GGYRCC 1 cut(s) 3024
BspTI CTTAAG 1 cut(s) 2452
BsrBI CCGCTC 2 cut(s) 816, 2242
BsrDI GCAATG 1 cut(s) 875
BsrFI RCCGGY 8 cut(s) 512, 692, 893, 1369, 1394, 2060, 3253, 3585
BsrI ACTGG 4 cut(s) 397, 1836, 2650, 3144
BssAI RCCGGY 8 cut(s) 512, 692, 893, 1369, 1394, 2060, 3253, 3585
BssECI CCNNGG 5 cut(s) 175, 267, 2086, 2580, 2658
BssMI GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
BssSI CACGAG 3 cut(s) 1900, 3299, 3659
BssT1I CCWWGG 3 cut(s) 175, 2086, 2658
Bst2BI CACGAG 3 cut(s) 1900, 3299, 3659
Bst2UI CCWGG 1 cut(s) 3681
Bst6I CTCTTC 5 cut(s) 320, 702, 806, 3116, 3221
BstAFI CTTAAG 1 cut(s) 2452
BstAPI GCANNNNNTGC 5 cut(s) 1379, 2357, 2429, 3347, 3500
BstBAI YACGTR 1 cut(s) 2609
BstDEI CTNAG 9 cut(s) 1071, 1506, 1527, 1761, 1855, 2517, 2693, 2852, 3519
BstDSI CCRYGG 2 cut(s) 175, 2580
BstF5I GGATG 3 cut(s) 1306, 1726, 2320
BstFNI CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
BstH2I RGCGCY 2 cut(s) 1620, 2379
BstHHI GCGC 9 cut(s) 90, 376, 528, 869, 1554, 1619, 2014, 2378, 2758
BstKTI GATC 9 cut(s) 222, 1435, 1440, 1960, 2900, 2962, 2969, 3469, 3573
BstMAI GTCTC 3 cut(s) 438, 2805, 3692
BstMBI GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
BstNI CCWGG 1 cut(s) 3681
BstNSI RCATGY 1 cut(s) 1841
BstSCI CCNGG 4 cut(s) 266, 267, 1716, 3679
BstSFI CTRYAG 4 cut(s) 1389, 1683, 2210, 3710
BstSLI GKGCMC 1 cut(s) 3300
BstSNI TACGTA 1 cut(s) 2609
BstUI CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
BstV2I GAAGAC 2 cut(s) 1425, 3682
BstX2I RGATCY 1 cut(s) 3466
BstYI RGATCY 1 cut(s) 3466
Bsu15I ATCGAT 1 cut(s) 222
BsuI GTATCC 3 cut(s) 182, 2936, 3182
BsuTUI ATCGAT 1 cut(s) 222
BtgI CCRYGG 2 cut(s) 175, 2580
BtrI CACGTC 2 cut(s) 2101, 2847
BtsCI GGATG 3 cut(s) 1306, 1726, 2320
BtsI GCAGTG 2 cut(s) 1578, 2430
BtuMI TCGCGA 1 cut(s) 1893
BveI ACCTGC 2 cut(s) 617, 1816
CaiI CAGNNNCTG 3 cut(s) 1831, 2429, 3518
CciI TCATGA 1 cut(s) 1038
CfoI GCGC 9 cut(s) 90, 376, 528, 869, 1554, 1619, 2014, 2378, 2758
Cfr10I RCCGGY 8 cut(s) 512, 692, 893, 1369, 1394, 2060, 3253, 3585
Cfr13I GGNCC 6 cut(s) 793, 1220, 2912, 3104, 3529, 3559
Cfr9I CCCGGG 1 cut(s) 267
ClaI ATCGAT 1 cut(s) 222
CseI GACGC 1 cut(s) 1430
CsiI ACCWGGT 1 cut(s) 3679
CspAI ACCGGT 1 cut(s) 3585
CspCI CAANNNNNGTGG 2 cut(s) 2562, 2597
DdeI CTNAG 9 cut(s) 1071, 1506, 1527, 1761, 1855, 2517, 2693, 2852, 3519
DpnI GATC 9 cut(s) 221, 1434, 1439, 1959, 2899, 2961, 2968, 3468, 3572
DpnII GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
DraIII CACNNNGTG 1 cut(s) 2048
DrdI GACNNNNNNGTC 1 cut(s) 3684
DriI GACNNNNNGTC 2 cut(s) 1786, 3514
DseDI GACNNNNNNGTC 1 cut(s) 3684
EaeI YGGCCR 4 cut(s) 514, 979, 2058, 2943
Eam1104I CTCTTC 5 cut(s) 320, 702, 806, 3116, 3221
Eam1105I GACNNNNNGTC 2 cut(s) 1786, 3514
EarI CTCTTC 5 cut(s) 320, 702, 806, 3116, 3221
EciI GGCGGA 1 cut(s) 146
Eco105I TACGTA 1 cut(s) 2609
Eco130I CCWWGG 3 cut(s) 175, 2086, 2658
Eco32I GATATC 3 cut(s) 2643, 3043, 3196
Eco47I GGWCC 2 cut(s) 1220, 3529
Eco47III AGCGCT 2 cut(s) 1618, 2377
Eco57I CTGAAG 5 cut(s) 456, 726, 946, 2552, 3396
Eco88I CYCGRG 5 cut(s) 76, 267, 1658, 2015, 2036
EcoO109I RGGNCCY 1 cut(s) 793
EcoRII CCWGG 1 cut(s) 3679
EcoRV GATATC 3 cut(s) 2643, 3043, 3196
EcoT14I CCWWGG 3 cut(s) 175, 2086, 2658
EcoT22I ATGCAT 1 cut(s) 2776
ErhI CCWWGG 3 cut(s) 175, 2086, 2658
FalI AAGNNNNNCTT 7 cut(s) 32, 2061, 2093, 2268, 2300, 3525, 3557
FaqI GGGAC 2 cut(s) 2180, 2561
FauI CCCGC 1 cut(s) 54
FauNDI CATATG 1 cut(s) 850
FbaI TGATCA 1 cut(s) 1957
FblI GTMKAC 2 cut(s) 653, 3709
FokI GGATG 3 cut(s) 1313, 1733, 2327
FspBI CTAG 6 cut(s) 485, 1224, 2124, 2381, 2870, 3239
GlaI GCGC 9 cut(s) 89, 375, 527, 868, 1553, 1618, 2013, 2377, 2757
GsaI CCCAGC 1 cut(s) 806
GsuI CTGGAG 2 cut(s) 380, 2633
HaeII RGCGCY 2 cut(s) 1620, 2379
HgaI GACGC 1 cut(s) 1430
HhaI GCGC 9 cut(s) 90, 376, 528, 869, 1554, 1619, 2014, 2378, 2758
Hin6I GCGC 9 cut(s) 88, 374, 526, 867, 1552, 1617, 2012, 2376, 2756
HinP1I GCGC 9 cut(s) 88, 374, 526, 867, 1552, 1617, 2012, 2376, 2756
HincII GTYRAC 1 cut(s) 3594
HindII GTYRAC 1 cut(s) 3594
HphI GGTGA 6 cut(s) 515, 683, 1828, 2440, 3037, 3142
Hpy166II GTNNAC 8 cut(s) 567, 654, 736, 846, 1710, 1726, 3594, 3710
Hpy8I GTNNAC 8 cut(s) 567, 654, 736, 846, 1710, 1726, 3594, 3710
Hpy99I CGWCG 2 cut(s) 3475, 3571
HpyAV CCTTC 7 cut(s) 820, 1475, 1511, 2527, 2836, 2887, 3371
HpyCH4IV ACGT 4 cut(s) 596, 2100, 2608, 2846
HpyF3I CTNAG 9 cut(s) 1071, 1506, 1527, 1761, 1855, 2517, 2693, 2852, 3519
HpySE526I ACGT 4 cut(s) 596, 2100, 2608, 2846
HspAI GCGC 9 cut(s) 88, 374, 526, 867, 1552, 1617, 2012, 2376, 2756
Kpn2I TCCGGA 1 cut(s) 1535
KpnI GGTACC 1 cut(s) 3028
KroI GCCGGC 5 cut(s) 692, 893, 1394, 2060, 3253
KroNI GCCGGC 5 cut(s) 694, 895, 1396, 2062, 3255
Ksp22I TGATCA 1 cut(s) 1957
Kzo9I GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
LguI GCTCTTC 1 cut(s) 806
MabI ACCWGGT 1 cut(s) 3679
MaeI CTAG 6 cut(s) 485, 1224, 2124, 2381, 2870, 3239
MaeII ACGT 4 cut(s) 596, 2100, 2608, 2846
MaeIII GTNAC 8 cut(s) 503, 1335, 1904, 2040, 2425, 2842, 2847, 3325
MalI GATC 9 cut(s) 221, 1434, 1439, 1959, 2899, 2961, 2968, 3468, 3572
MbiI CCGCTC 2 cut(s) 816, 2242
MboI GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
MfeI CAATTG 2 cut(s) 1005, 3444
MflI RGATCY 1 cut(s) 3466
MhlI GDGCHC 4 cut(s) 1244, 2335, 2910, 3300
MlyI GAGTC 3 cut(s) 300, 2792, 3524
MmeI TCCRAC 3 cut(s) 48, 1278, 1348
Mph1103I ATGCAT 1 cut(s) 2776
MroI TCCGGA 1 cut(s) 1535
MroNI GCCGGC 5 cut(s) 692, 893, 1394, 2060, 3253
MroXI GAANNNNTTC 2 cut(s) 935, 2537
MseI TTAA 6 cut(s) 143, 1842, 2267, 2453, 2837, 3739
MslI CAYNNNNRTG 6 cut(s) 180, 325, 574, 1043, 1987, 2955
MspA1I CMGCKG 3 cut(s) 2471, 3332, 3667
MspCI CTTAAG 1 cut(s) 2452
MspR9I CCNGG 4 cut(s) 268, 269, 1718, 3681
MunI CAATTG 2 cut(s) 1005, 3444
Mva1269I GAATGC 4 cut(s) 341, 935, 3352, 3375
MvaI CCWGG 1 cut(s) 3681
MvnI CGCG 5 cut(s) 376, 1893, 2256, 2589, 2741
NaeI GCCGGC 5 cut(s) 694, 895, 1396, 2062, 3255
NciI CCSGG 3 cut(s) 268, 269, 1718
NcoI CCATGG 1 cut(s) 175
NdeI CATATG 1 cut(s) 850
NdeII GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
NgoMIV GCCGGC 5 cut(s) 692, 893, 1394, 2060, 3253
NlaIV GGNNCC 2 cut(s) 609, 3026
NmeAIII GCCGAG 2 cut(s) 957, 2889
NmuCI GTSAC 6 cut(s) 503, 2040, 2425, 2842, 2847, 3325
NruI TCGCGA 1 cut(s) 1893
NsiI ATGCAT 1 cut(s) 2776
NspI RCATGY 1 cut(s) 1841
PaeR7I CTCGAG 1 cut(s) 1658
PagI TCATGA 1 cut(s) 1038
PaqCI CACCTGC 1 cut(s) 617
PciI ACATGT 1 cut(s) 1837
PciSI GCTCTTC 1 cut(s) 806
PcsI WCGNNNNNNNCGW 2 cut(s) 120, 593
PctI GAATGC 4 cut(s) 341, 935, 3352, 3375
PdiI GCCGGC 5 cut(s) 694, 895, 1396, 2062, 3255
PdmI GAANNNNTTC 2 cut(s) 935, 2537
PfoI TCCNGGA 1 cut(s) 1716
PinAI ACCGGT 1 cut(s) 3585
PleI GAGTC 3 cut(s) 300, 2792, 3523
PpsI GAGTC 3 cut(s) 300, 2792, 3523
Ppu21I YACGTR 1 cut(s) 2609
PscI ACATGT 1 cut(s) 1837
PshBI ATTAAT 1 cut(s) 2267
Psp6I CCWGG 1 cut(s) 3679
PspFI CCCAGC 1 cut(s) 802
PspGI CCWGG 1 cut(s) 3679
PspN4I GGNNCC 2 cut(s) 609, 3026
PspPI GGNCC 6 cut(s) 793, 1220, 2912, 3104, 3529, 3559
PstI CTGCAG 1 cut(s) 2214
PstNI CAGNNNCTG 3 cut(s) 1831, 2429, 3518
PsuI RGATCY 1 cut(s) 3466
PvuII CAGCTG 2 cut(s) 2471, 3667
RruI TCGCGA 1 cut(s) 1893
RseI CAYNNNNRTG 6 cut(s) 180, 325, 574, 1043, 1987, 2955
SapI GCTCTTC 1 cut(s) 806
SaqAI TTAA 6 cut(s) 143, 1842, 2267, 2453, 2837, 3739
Sau3AI GATC 9 cut(s) 219, 1432, 1437, 1957, 2897, 2959, 2966, 3466, 3570
Sau96I GGNCC 6 cut(s) 793, 1220, 2912, 3104, 3529, 3559
ScaI AGTACT 1 cut(s) 2781
SchI GAGTC 3 cut(s) 300, 2792, 3524
ScrFI CCNGG 4 cut(s) 268, 269, 1718, 3681
SduI GDGCHC 4 cut(s) 1244, 2335, 2910, 3300
SexAI ACCWGGT 1 cut(s) 3679
SfcI CTRYAG 4 cut(s) 1389, 1683, 2210, 3710
Sfr274I CTCGAG 1 cut(s) 1658
SinI GGWCC 2 cut(s) 1220, 3529
SlaI CTCGAG 1 cut(s) 1658
SmaI CCCGGG 1 cut(s) 269
SmiMI CAYNNNNRTG 6 cut(s) 180, 325, 574, 1043, 1987, 2955
SmlI CTYRAG 3 cut(s) 797, 1658, 2452
SmoI CTYRAG 3 cut(s) 797, 1658, 2452
SnaBI TACGTA 1 cut(s) 2609
SsiI CCGC 7 cut(s) 47, 131, 251, 816, 1913, 2242, 3332
SspMI CTAG 6 cut(s) 485, 1224, 2124, 2381, 2870, 3239
StyD4I CCNGG 4 cut(s) 266, 267, 1716, 3679
StyI CCWWGG 3 cut(s) 175, 2086, 2658
TaiI ACGT 4 cut(s) 599, 2103, 2611, 2849
TatI WGTACW 3 cut(s) 288, 735, 2779
TauI GCSGC 2 cut(s) 2245, 3335
Tru1I TTAA 6 cut(s) 143, 1842, 2267, 2453, 2837, 3739
Tru9I TTAA 6 cut(s) 143, 1842, 2267, 2453, 2837, 3739
TseFI GTSAC 6 cut(s) 503, 2040, 2425, 2842, 2847, 3325
Tsp45I GTSAC 6 cut(s) 503, 2040, 2425, 2842, 2847, 3325
TspDTI ATGAA 8 cut(s) 401, 604, 1323, 1465, 2818, 2906, 3221, 3349
TspGWI ACGGA 6 cut(s) 54, 600, 939, 2569, 2653, 3459
TspMI CCCGGG 1 cut(s) 267
Vha464I CTTAAG 1 cut(s) 2452
VpaK11BI GGWCC 2 cut(s) 1220, 3529
VspI ATTAAT 1 cut(s) 2267
XapI RAATTY 4 cut(s) 421, 1174, 2803, 3064
XceI RCATGY 1 cut(s) 1841
XhoI CTCGAG 1 cut(s) 1658
XmaI CCCGGG 1 cut(s) 267
XmiI GTMKAC 2 cut(s) 653, 3709
XmnI GAANNNNTTC 2 cut(s) 935, 2537
XspI CTAG 6 cut(s) 485, 1224, 2124, 2381, 2870, 3239
ZrmI AGTACT 1 cut(s) 2781
Zsp2I ATGCAT 1 cut(s) 2776
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.