Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0000772

Physical Location & Seq

Genomic Coordinates Forward (+)

10769722 .. 10770775

Gene Structure 1054 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT1G30455.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 765 bp

ATGCTCTACCTTACAGAAAACCGCAAGTATGAGAACCTTGTTGATCCTTCAACTTTCATTCCTCGATTCTCAAACAAGTTATTGAAAGGTGCACACAATAAGCAAGTCGTGGAAACGGCTACACACATTATAGCTAGTATGAAGAGTAACTGGATGCAGACCGGACGGAAACCAAGTGGAATATGTGGAGCAGCACTCTATACAGCTGCACTTTCTCATGGTGGCTCGGATCCTCCCTCCTTCCAGCGAGCAGAGAAAGAGAGAATGGAGGAAAAAGCTTCTACAGAAGAAAACGACAAACAACAAAATTCGGATGAATCAAGCACTTTGTCTGACCTCGATGATGGAGAGTTGGATTGTTATTTTCGGAACCCGAAAGAAGTGCACCTGGTTGAGATTATCTTTGATCACGAAAACCCCGGATATGATGAGAAGGAAGCAGCAGCTTTAAACGCTTGCAATAATGCAAGCAACCTTTTCGAAGCTTCTAAAGCAGCGGCGGCGAAATCTAGAAAAGATAAACGACAACAACGTGCTGAGGAAGAAAAGAACGCGCCTCCTCCAGCTACAGCCATGGAAGCTGTTGATAGCATGGTCAAGAGAAAGAAATTTCCCGATACTAACTGCGATTATTTGGAGGAGCTTCTTGATACTTCAGCTCAGAAAGCATCCAAGAGATTGAAAACCGAGACAGTGATGGAGAAGAACAAGGAAGAGCATGAGATTGTTGAAAACGAACAGGATGAAGAAGATTACGCGGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000126 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006352 GO:0006383 GO:0006384 GO:0006399 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009303 GO:0009304 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090576 GO:0097659 GO:0098781 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRF1 TFIIB

Protein Analysis

254

Amino Acids

28.6

Weight (kDa)

5.01

Isoelectric Point (pI)

44.15

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIB	PF00382	12 - 73	8.6e-12	Transcription factor TFIIB repeat
BRF1	PF07741	111 - 216	3.3e-17	Brf1-like TBP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30455 AT2G01280 AT2G01280 AT2G01280 AT2G45100 AT2G45100 AT2G45100 AT3G09360 AT3G09360 AT3G09360
fragaria_vesca	FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_7g06241
malus_domestica	MD04G1033900.v1.1
prunus_persica	Prupe.1G180700_v2.0.a1
pyrus_communis	pycom04g02830
rosa_chinensis	RchiOBHm_Chr4g0409121 RchiOBHm_Chr7g0228131
rosa_laevigata	RLG00000001586 RLG00000008590
rosa_multiflora	Rmu_sc0001348.1_g000036 Rmu_sc0001348.1_g000042 Rmu_sc0001526.1_g000006 Rmu_sc0001526.1_g000008 Rmu_sc0012031.1_g000013 Rmu_sc0021826.1_g000001 Rmu_ssc0000372.1_g000072
rosa_roxburghii	Rroxscaffold_2G00115130 Rroxscaffold_3G00231370 Rroxscaffold_5G00353680 Rroxscaffold_7G00191300
rosa_rugosa	Rorug04G0090500 Rorug04G0090600 Rorug04G0090700 Rorug07G0245700 Rorug07G0245800
rosa_samantha	Rh2AG319000 Rh4AG154400 Rh4BG152200 Rh4CG163600 Rh4CG264400 Rh4DG147400 Rh5DG375300 Rh7AG400000 Rh7BG381300 Rh7CG418200 Rh7DG396100
rosa_wichuraiana	Rw0G005090 Rw0G011060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	554, 758
AciI	CCGC	4 cut(s)	22, 497, 500, 758
AclWI	GGATC	3 cut(s)	38, 224, 237
AcsI	RAATTY	2 cut(s)	307, 608
AcuI	CTGAAG	1 cut(s)	639
AgsI	TTSAA	4 cut(s)	51, 85, 682, 731
AjnI	CCWGG	1 cut(s)	387
AluBI	AGCT	9 cut(s)	134, 206, 278, 446, 485, 566, 581, 643, 659
AluI	AGCT	9 cut(s)	134, 206, 278, 446, 485, 566, 581, 643, 659
Alw21I	GWGCWC	2 cut(s)	94, 387
Alw26I	GTCTC	1 cut(s)	683
Alw44I	GTGCAC	2 cut(s)	90, 383
AlwI	GGATC	3 cut(s)	38, 224, 237
ApaLI	GTGCAC	2 cut(s)	90, 383
ApeKI	GCWGC	5 cut(s)	191, 206, 440, 443, 494
ApoI	RAATTY	2 cut(s)	307, 608
AspLEI	GCGC	1 cut(s)	556
AsuC2I	CCSGG	1 cut(s)	420
AsuII	TTCGAA	1 cut(s)	480
BaeGI	GKGCMC	2 cut(s)	94, 387
BamHI	GGATCC	1 cut(s)	229
Bbv12I	GWGCWC	2 cut(s)	94, 387
BbvCI	CCTCAGC	1 cut(s)	537
BbvI	GCAGC	5 cut(s)	193, 203, 452, 455, 506
BccI	CCATC	2 cut(s)	338, 691
BceAI	ACGGC	1 cut(s)	132
BcgI	CGANNNNNNTGC	4 cut(s)	364, 398, 460, 494
BciT130I	CCWGG	1 cut(s)	389
BclI	TGATCA	1 cut(s)	406
BcnI	CCSGG	1 cut(s)	420
BcoDI	GTCTC	1 cut(s)	683
BfaI	CTAG	2 cut(s)	135, 510
BfmI	CTRYAG	2 cut(s)	282, 567
BisI	GCNGC	8 cut(s)	192, 207, 441, 444, 495, 498, 501, 759
BlsI	GCNGC	8 cut(s)	193, 208, 442, 445, 496, 499, 502, 760
Bme1390I	CCNGG	2 cut(s)	389, 420
BmiI	GGNNCC	2 cut(s)	231, 371
BmrFI	CCNGG	2 cut(s)	389, 420
BmsI	GCATC	2 cut(s)	144, 677
BplI	GAGNNNNNCTC	2 cut(s)	180, 212
BpmI	CTGGAG	1 cut(s)	546
Bpu10I	CCTNAGC	1 cut(s)	537
Bpu14I	TTCGAA	1 cut(s)	480
BpuMI	CCSGG	1 cut(s)	420
BsaJI	CCNNGG	2 cut(s)	418, 573
BsaWI	WCCGGW	1 cut(s)	161
Bse1I	ACTGG	1 cut(s)	155
BseBI	CCWGG	1 cut(s)	389
BseDI	CCNNGG	2 cut(s)	418, 573
BseGI	GGATG	4 cut(s)	159, 319, 668, 748
BseMII	CTCAG	2 cut(s)	528, 674
BseNI	ACTGG	1 cut(s)	155
BseRI	GAGGAG	2 cut(s)	549, 653
BseSI	GKGCMC	2 cut(s)	94, 387
BseXI	GCAGC	5 cut(s)	193, 203, 452, 455, 506
BsgI	GTGCAG	1 cut(s)	192
Bsh1236I	CGCG	2 cut(s)	554, 758
BsiHKAI	GWGCWC	2 cut(s)	94, 387
BsiSI	CCGG	2 cut(s)	162, 420
BsmAI	GTCTC	1 cut(s)	683
Bsp119I	TTCGAA	1 cut(s)	480
Bsp1286I	GDGCHC	2 cut(s)	94, 387
Bsp143I	GATC	3 cut(s)	43, 229, 406
Bsp19I	CCATGG	1 cut(s)	573
BspACI	CCGC	4 cut(s)	22, 497, 500, 758
BspCNI	CTCAG	2 cut(s)	529, 673
BspFNI	CGCG	2 cut(s)	554, 758
BspLI	GGNNCC	2 cut(s)	231, 371
BspPI	GGATC	3 cut(s)	38, 224, 237
BspQI	GCTCTTC	1 cut(s)	708
BspT104I	TTCGAA	1 cut(s)	480
BsrI	ACTGG	1 cut(s)	155
BssECI	CCNNGG	2 cut(s)	418, 573
BssMI	GATC	3 cut(s)	43, 229, 406
BssT1I	CCWWGG	1 cut(s)	573
Bst2UI	CCWGG	1 cut(s)	389
Bst4CI	ACNGT	1 cut(s)	694
Bst6I	CTCTTC	2 cut(s)	137, 708
BstBI	TTCGAA	1 cut(s)	480
BstC8I	GCNNGC	3 cut(s)	249, 457, 469
BstDEI	CTNAG	2 cut(s)	537, 660
BstDSI	CCRYGG	1 cut(s)	573
BstF5I	GGATG	4 cut(s)	159, 319, 668, 748
BstFNI	CGCG	2 cut(s)	554, 758
BstHHI	GCGC	1 cut(s)	556
BstKTI	GATC	3 cut(s)	46, 232, 409
BstMAI	GTCTC	1 cut(s)	683
BstMBI	GATC	3 cut(s)	43, 229, 406
BstMWI	GCNNNNNNNGC	5 cut(s)	452, 491, 500, 578, 665
BstNI	CCWGG	1 cut(s)	389
BstSCI	CCNGG	2 cut(s)	387, 418
BstSFI	CTRYAG	2 cut(s)	282, 567
BstSLI	GKGCMC	2 cut(s)	94, 387
BstUI	CGCG	2 cut(s)	554, 758
BstV1I	GCAGC	5 cut(s)	193, 203, 452, 455, 506
BstX2I	RGATCY	1 cut(s)	229
BstYI	RGATCY	1 cut(s)	229
BtgI	CCRYGG	1 cut(s)	573
BtsCI	GGATG	4 cut(s)	159, 319, 668, 748
BtsIMutI	CAGTG	1 cut(s)	699
Cac8I	GCNNGC	3 cut(s)	249, 457, 469
CfoI	GCGC	1 cut(s)	556
CsiI	ACCWGGT	1 cut(s)	387
CviAII	CATG	5 cut(s)	218, 574, 592, 719, 762
DdeI	CTNAG	2 cut(s)	537, 660
DpnI	GATC	3 cut(s)	45, 231, 408
DpnII	GATC	3 cut(s)	43, 229, 406
DraI	TTTAAA	1 cut(s)	450
Eam1104I	CTCTTC	2 cut(s)	137, 708
EarI	CTCTTC	2 cut(s)	137, 708
Eco130I	CCWWGG	1 cut(s)	573
Eco57I	CTGAAG	1 cut(s)	639
EcoRII	CCWGG	1 cut(s)	387
EcoT14I	CCWWGG	1 cut(s)	573
ErhI	CCWWGG	1 cut(s)	573
FaeI	CATG	5 cut(s)	221, 577, 595, 722, 765
FatI	CATG	5 cut(s)	217, 573, 591, 718, 761
FbaI	TGATCA	1 cut(s)	406
Fnu4HI	GCNGC	8 cut(s)	192, 207, 441, 444, 495, 498, 501, 759
FokI	GGATG	4 cut(s)	166, 326, 655, 755
Fsp4HI	GCNGC	8 cut(s)	192, 207, 441, 444, 495, 498, 501, 759
FspBI	CTAG	2 cut(s)	135, 510
GlaI	GCGC	1 cut(s)	555
GluI	GCNGC	8 cut(s)	192, 207, 441, 444, 495, 498, 501, 759
GsuI	CTGGAG	1 cut(s)	546
HapII	CCGG	2 cut(s)	162, 420
HhaI	GCGC	1 cut(s)	556
Hin1II	CATG	5 cut(s)	221, 577, 595, 722, 765
Hin6I	GCGC	1 cut(s)	554
HinP1I	GCGC	1 cut(s)	554
HindIII	AAGCTT	2 cut(s)	276, 483
HinfI	GANTC	2 cut(s)	66, 317
HpaII	CCGG	2 cut(s)	162, 420
Hpy166II	GTNNAC	2 cut(s)	92, 385
Hpy188I	TCNGA	5 cut(s)	229, 313, 334, 369, 663
Hpy188III	TCNNGA	5 cut(s)	410, 510, 598, 614, 647
Hpy8I	GTNNAC	2 cut(s)	92, 385
HpyAV	CCTTC	3 cut(s)	57, 250, 427
HpyCH4III	ACNGT	1 cut(s)	694
HpyCH4IV	ACGT	1 cut(s)	532
HpyCH4V	TGCA	6 cut(s)	92, 157, 209, 385, 459, 467
HpyF10VI	GCNNNNNNNGC	5 cut(s)	452, 491, 500, 578, 665
HpyF3I	CTNAG	2 cut(s)	537, 660
HpySE526I	ACGT	1 cut(s)	532
Hsp92II	CATG	5 cut(s)	221, 577, 595, 722, 765
HspAI	GCGC	1 cut(s)	554
Ksp22I	TGATCA	1 cut(s)	406
Kzo9I	GATC	3 cut(s)	43, 229, 406
LguI	GCTCTTC	1 cut(s)	708
LmnI	GCTCC	2 cut(s)	188, 640
LpnPI	CCDG	8 cut(s)	136, 175, 257, 374, 401, 433, 576, 725
Lsp1109I	GCAGC	5 cut(s)	193, 203, 452, 455, 506
LweI	GCATC	2 cut(s)	144, 677
MabI	ACCWGGT	1 cut(s)	387
MaeI	CTAG	2 cut(s)	135, 510
MaeII	ACGT	1 cut(s)	532
MaeIII	GTNAC	1 cut(s)	146
MalI	GATC	3 cut(s)	45, 231, 408
MboI	GATC	3 cut(s)	43, 229, 406
MboII	GAAGA	7 cut(s)	154, 299, 554, 715, 725, 758, 761
MflI	RGATCY	1 cut(s)	229
MhlI	GDGCHC	2 cut(s)	94, 387
MluCI	AATT	2 cut(s)	307, 608
MmeI	TCCRAC	1 cut(s)	333
MnlI	CCTC	9 cut(s)	72, 243, 247, 262, 347, 532, 567, 570, 631
MseI	TTAA	1 cut(s)	449
MspA1I	CMGCKG	2 cut(s)	206, 497
MspI	CCGG	2 cut(s)	162, 420
MspR9I	CCNGG	2 cut(s)	389, 420
MvaI	CCWGG	1 cut(s)	389
MvnI	CGCG	2 cut(s)	554, 758
MwoI	GCNNNNNNNGC	5 cut(s)	452, 491, 500, 578, 665
NciI	CCSGG	1 cut(s)	420
NcoI	CCATGG	1 cut(s)	573
NdeII	GATC	3 cut(s)	43, 229, 406
NlaIII	CATG	5 cut(s)	221, 577, 595, 722, 765
NlaIV	GGNNCC	2 cut(s)	231, 371
NspV	TTCGAA	1 cut(s)	480
PciSI	GCTCTTC	1 cut(s)	708
PcsI	WCGNNNNNNNCGW	1 cut(s)	529
PfeI	GAWTC	2 cut(s)	66, 317
PkrI	GCNGC	8 cut(s)	193, 208, 442, 445, 496, 499, 502, 760
Psp6I	CCWGG	1 cut(s)	387
PspGI	CCWGG	1 cut(s)	387
PspN4I	GGNNCC	2 cut(s)	231, 371
PsuI	RGATCY	1 cut(s)	229
PvuII	CAGCTG	1 cut(s)	206
SapI	GCTCTTC	1 cut(s)	708
SaqAI	TTAA	1 cut(s)	449
SatI	GCNGC	8 cut(s)	192, 207, 441, 444, 495, 498, 501, 759
Sau3AI	GATC	3 cut(s)	43, 229, 406
ScrFI	CCNGG	2 cut(s)	389, 420
SduI	GDGCHC	2 cut(s)	94, 387
SexAI	ACCWGGT	1 cut(s)	387
SfaNI	GCATC	2 cut(s)	144, 677
SfcI	CTRYAG	2 cut(s)	282, 567
SfuI	TTCGAA	1 cut(s)	480
Sse9I	AATT	2 cut(s)	307, 608
SsiI	CCGC	4 cut(s)	22, 497, 500, 758
SspMI	CTAG	2 cut(s)	135, 510
StyD4I	CCNGG	2 cut(s)	387, 418
StyI	CCWWGG	1 cut(s)	573
TaaI	ACNGT	1 cut(s)	694
TaiI	ACGT	1 cut(s)	535
TaqI	TCGA	3 cut(s)	64, 339, 480
TasI	AATT	2 cut(s)	307, 608
TauI	GCSGC	3 cut(s)	500, 503, 761
TfiI	GAWTC	2 cut(s)	66, 317
Tru1I	TTAA	1 cut(s)	449
Tru9I	TTAA	1 cut(s)	449
TscAI	CASTG	1 cut(s)	699
TseI	GCWGC	5 cut(s)	191, 206, 440, 443, 494
TspDTI	ATGAA	4 cut(s)	46, 155, 330, 759
TspGWI	ACGGA	1 cut(s)	181
TspRI	CASTG	1 cut(s)	699
VneI	GTGCAC	2 cut(s)	90, 383
XapI	RAATTY	2 cut(s)	307, 608
XbaI	TCTAGA	1 cut(s)	509
XspI	CTAG	2 cut(s)	135, 510

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.