Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0000772

Physical Location & Seq

Genomic Coordinates Reverse (-)

29419028 .. 29433206

Gene Structure 14179 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG147400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGGTATGGTGTACTTTCTGTGTCAAAAATGTTGAAGGAACCAGCGATGCTGGTAGACTATGGTGTTCCGGATGCGGCAAGGTTTTAGAGGATCACATTTTCTCTGAGGAACCCACTTTCGTTAAGAATGCGGCGGGCCAGAGCCAATTGGCTGGAAGATATGTACGCTCCAGGGAAAGCGAAATCTCAGCTTCTCGCGAAAGGATACTCGAAAATGCCGAATATGAATTGAGATGTATGAGAAATGCTTTAGATATGGGAGACAACGAAGAAATTATTGACATAGCTAAACGCTTTTATAGAATTGCGATTGAGCGGAATTTCACAAGGGGCCGTAAGTCAGAGCAAGTACAGGCTGCATGTCTTTACATTGCATGTCGGGAAAAGAAGAAGCCATATCTTCTCATTGATTTTTCGAACTACTTGAAGATAAATGTCTATGTGCTGGGAGCTGTGTTTTTGCAACTTTGCAAGGCTCTGAGGCTTGATGAGCACCCAATTGTTCAAAAACTTGTTGATCCTTCCTGGTTTATTCATAGATTTGCTGAGAGTTTACCAGGAGGAAGGGATAAAGGGGTTATGCAAACTGCACAAAGGATTATCACAAGCATGAAACGGGATTGGATGCAGACAGGCAGAAAACCCAGTGGACTATGTGGTGCTGCATTATATATATCAGCACTTTCACACGGTCTCAAGTGCTCCAAATCTGATATTATCAAAATTGTACATGTTTGTGATGCTACATTGACTAAGCGGCTGGTTGAATTTGAGAATACAGAATCTGGGAGCTTGACGATTGAAGAGTTCCTCGTGAAGGCAAAGGAACAGGATGAATTAAGCAAACAACCAATTATGGCTGATAAAATGGATAATACAGATGGTGGCCTTTGTGAACTCAAGATCCTTTGTGAACATAAGGATAGTGGAAAACCTTTTGCGTATGGACTTTGTCAAACCTGTTATGATGATTTTATGACAATTTCAGGTGGACTTGACGGAGGATCTAATCCTCCTGCTTTCCAGCGTGCTGAAATAGAGAGATTGAAAAAAGCATCTGCAGAGCTTAATGCTAATGACTCTGGCATCGATACCATTGCTTGTCAAAGCCTTGACAACAGTGATCAGTCATCCAAGTTTGAGAAAGAGAACAACACAAAATCAGGGAGTGAGAATAAAGGACTGCTGCTTATGGAGCCTGAAAGTGATGGGGCTGCTATGGAGAATACAGCTGTTGAGACCGGAACTGAAGCTTGTGATGATGAGTCTGACAACTTTTCAGATATTGATGATCTGGAGGTTGACGGCTACCTTCTTAATGAAGAGGGGAAACGCTACAAAAAGATGATTTGGGAAGAAATGAATCGTGAGTACATTGAGGAGCAAGCAGCGAAGGAGGAAGCAGCTAAGGCTGCTATGGATATCAATTGTCCAGAAGGTGCAAAGAAACTTGCGCAAAATACTGAAGCAGCTGTTGCCAAGAGAAAAAAGGAGAGGCAACTAGCTAGAGCAGCTGAAGCGAAAAACTCTACTCCAGCTCAGACTGCTGCAGAAGCTGTCCACCAAATGCTAACGAAGAAGAGACTCAGCTCAAAAATCAACTTTGATGTCCTGGAGAATATGTTCGAAAAATCTGTATGCATCTCTCCTTATTGGTTTCTCTTATTAAATGACAACGAATTACTTTTTCATTTCTTTGCATGCATGCCCATGACAAGTTCCATAGATGCCAGAAATGAAGTAGTAACAGATTGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000126 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006352 GO:0006383 GO:0006384 GO:0006399 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009303 GO:0009304 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090576 GO:0097659 GO:0098781 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRF1 TFIIB

Protein Analysis

585

Amino Acids

65.59

Weight (kDa)

5.2

Isoelectric Point (pI)

43.16

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIB	PF00382	69 - 158	5.8e-16	Transcription factor TFIIB repeat
TFIIB	PF00382	169 - 259	1.9e-17	Transcription factor TFIIB repeat
BRF1	PF07741	427 - 543	4.4e-25	Brf1-like TBP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30455 AT2G01280 AT2G01280 AT2G01280 AT2G45100 AT2G45100 AT2G45100 AT3G09360 AT3G09360 AT3G09360
fragaria_vesca	FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_7g06241
malus_domestica	MD04G1033900.v1.1
prunus_persica	Prupe.1G180700_v2.0.a1
pyrus_communis	pycom04g02830
rosa_chinensis	RchiOBHm_Chr4g0409121 RchiOBHm_Chr7g0228131
rosa_laevigata	RLG00000001586 RLG00000008590
rosa_multiflora	Rmu_sc0001348.1_g000036 Rmu_sc0001348.1_g000042 Rmu_sc0001526.1_g000006 Rmu_sc0001526.1_g000008 Rmu_sc0012031.1_g000013 Rmu_sc0021826.1_g000001 Rmu_ssc0000372.1_g000072
rosa_roxburghii	Rroxscaffold_2G00115130 Rroxscaffold_3G00231370 Rroxscaffold_5G00353680 Rroxscaffold_7G00191300
rosa_rugosa	Rorug04G0090500 Rorug04G0090600 Rorug04G0090700 Rorug07G0245700 Rorug07G0245800
rosa_samantha	Rh2AG319000 Rh4AG154400 Rh4BG152200 Rh4CG163600 Rh4CG264400 Rh4DG147400 Rh5DG375300 Rh7AG400000 Rh7BG381300 Rh7CG418200 Rh7DG396100
rosa_wichuraiana	Rw0G005090 Rw0G011060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1455
AccBSI	CCGCTC	1 cut(s)	316
AccI	GTMKAC	1 cut(s)	55
AccII	CGCG	1 cut(s)	198
AccIII	TCCGGA	1 cut(s)	68
AciI	CCGC	5 cut(s)	75, 131, 134, 316, 757
AclWI	GGATC	4 cut(s)	99, 512, 898, 1012
AcsI	RAATTY	2 cut(s)	319, 767
AcuI	CTGAAG	3 cut(s)	1269, 1485, 1536
AfaI	GTAC	5 cut(s)	13, 165, 351, 729, 1373
AfiI	CCNNNNNNNGG	1 cut(s)	817
AflIII	ACRYGT	1 cut(s)	730
AgsI	TTSAA	6 cut(s)	35, 427, 506, 767, 803, 1048
AjnI	CCWGG	4 cut(s)	170, 524, 556, 1611
AloI	GAACNNNNNNTCC	4 cut(s)	888, 920, 1607, 1639
Alw21I	GWGCWC	2 cut(s)	495, 704
Alw26I	GTCTC	4 cut(s)	255, 698, 1232, 1576
AlwI	GGATC	4 cut(s)	99, 512, 898, 1012
AlwNI	CAGNNNCTG	2 cut(s)	785, 1556
Aor13HI	TCCGGA	1 cut(s)	68
AoxI	GGCC	3 cut(s)	136, 331, 886
ApoI	RAATTY	2 cut(s)	319, 767
Asp700I	GAANNNNTTC	1 cut(s)	1622
AspLEI	GCGC	1 cut(s)	1456
AspS9I	GGNCC	2 cut(s)	136, 331
AsuII	TTCGAA	2 cut(s)	416, 1626
BauI	CACGAG	1 cut(s)	812
Bbv12I	GWGCWC	2 cut(s)	495, 704
BccI	CCATC	2 cut(s)	875, 1202
BceAI	ACGGC	2 cut(s)	318, 1321
BcgI	CGANNNNNNTGC	2 cut(s)	1079, 1113
BciT130I	CCWGG	4 cut(s)	172, 526, 558, 1613
BciVI	GTATCC	1 cut(s)	198
BclI	TGATCA	1 cut(s)	1123
BcoDI	GTCTC	4 cut(s)	255, 698, 1232, 1576
BfaI	CTAG	2 cut(s)	1502, 1506
BfmI	CTRYAG	2 cut(s)	1059, 1548
BfuI	GTATCC	1 cut(s)	198
Bme1390I	CCNGG	4 cut(s)	172, 526, 558, 1613
BmgT120I	GGNCC	2 cut(s)	136, 331
BmiI	GGNNCC	4 cut(s)	40, 111, 332, 1197
BmrFI	CCNGG	4 cut(s)	172, 526, 558, 1613
BmrI	ACTGGG	1 cut(s)	639
BmsI	GCATC	8 cut(s)	37, 62, 615, 730, 1064, 1095, 1650, 1717
BmuI	ACTGGG	1 cut(s)	639
BpmI	CTGGAG	4 cut(s)	154, 1316, 1518, 1634
Bpu10I	CCTNAGC	1 cut(s)	1407
Bpu14I	TTCGAA	2 cut(s)	416, 1626
BpuEI	CTTGAG	2 cut(s)	680, 884
Bsa29I	ATCGAT	1 cut(s)	1089
BsaI	GGTCTC	2 cut(s)	698, 1232
BsaJI	CCNNGG	1 cut(s)	171
BsaWI	WCCGGW	2 cut(s)	68, 1241
BsaXI	ACNNNNNCTCC	2 cut(s)	1607, 1637
Bsc4I	CCNNNNNNNGG	1 cut(s)	817
Bse1I	ACTGG	1 cut(s)	645
Bse3DI	GCAATG	2 cut(s)	369, 1095
BseAI	TCCGGA	1 cut(s)	68
BseBI	CCWGG	4 cut(s)	172, 526, 558, 1613
BseCI	ATCGAT	1 cut(s)	1089
BseDI	CCNNGG	1 cut(s)	171
BseGI	GGATG	4 cut(s)	77, 630, 838, 1130
BseLI	CCNNNNNNNGG	1 cut(s)	817
BseMI	GCAATG	2 cut(s)	369, 1095
BseMII	CTCAG	6 cut(s)	96, 201, 470, 537, 1553, 1600
BseNI	ACTGG	1 cut(s)	645
BseRI	GAGGAG	1 cut(s)	1394
BseYI	CCCAGC	1 cut(s)	445
BsgI	GTGCAG	1 cut(s)	573
Bsh1236I	CGCG	1 cut(s)	198
BshFI	GGCC	3 cut(s)	138, 333, 888
BshVI	ATCGAT	1 cut(s)	1089
BsiHKAI	GWGCWC	2 cut(s)	495, 704
BsiSI	CCGG	2 cut(s)	69, 1242
BslI	CCNNNNNNNGG	1 cut(s)	817
BsmAI	GTCTC	4 cut(s)	255, 698, 1232, 1576
BsmI	GAATGC	1 cut(s)	133
BsnI	GGCC	3 cut(s)	138, 333, 888
Bso31I	GGTCTC	2 cut(s)	698, 1232
Bsp119I	TTCGAA	2 cut(s)	416, 1626
Bsp1286I	GDGCHC	2 cut(s)	495, 704
Bsp13I	TCCGGA	1 cut(s)	68
Bsp1407I	TGTACA	1 cut(s)	727
Bsp143I	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
Bsp68I	TCGCGA	1 cut(s)	198
BspACI	CCGC	5 cut(s)	75, 131, 134, 316, 757
BspANI	GGCC	3 cut(s)	138, 333, 888
BspCNI	CTCAG	6 cut(s)	97, 200, 471, 538, 1552, 1599
BspDI	ATCGAT	1 cut(s)	1089
BspEI	TCCGGA	1 cut(s)	68
BspFNI	CGCG	1 cut(s)	198
BspLI	GGNNCC	4 cut(s)	40, 111, 332, 1197
BspMAI	CTGCAG	2 cut(s)	1063, 1552
BspPI	GGATC	4 cut(s)	99, 512, 898, 1012
BspT104I	TTCGAA	2 cut(s)	416, 1626
BspTNI	GGTCTC	2 cut(s)	698, 1232
BsrBI	CCGCTC	1 cut(s)	316
BsrDI	GCAATG	2 cut(s)	369, 1095
BsrGI	TGTACA	1 cut(s)	727
BsrI	ACTGG	1 cut(s)	645
BssECI	CCNNGG	1 cut(s)	171
BssMI	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
BssSI	CACGAG	1 cut(s)	812
Bst2BI	CACGAG	1 cut(s)	812
Bst2UI	CCWGG	4 cut(s)	172, 526, 558, 1613
Bst4CI	ACNGT	2 cut(s)	692, 1121
Bst6I	CTCTTC	3 cut(s)	798, 1317, 1574
BstAPI	GCANNNNNTGC	1 cut(s)	1475
BstAUI	TGTACA	1 cut(s)	727
BstBI	TTCGAA	2 cut(s)	416, 1626
BstC8I	GCNNGC	5 cut(s)	136, 1029, 1386, 1702, 1706
BstDEI	CTNAG	8 cut(s)	105, 187, 479, 546, 753, 1407, 1539, 1586
BstENI	CCTNNNNNAGG	1 cut(s)	815
BstF5I	GGATG	4 cut(s)	77, 630, 838, 1130
BstFNI	CGCG	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	1456
BstKTI	GATC	6 cut(s)	94, 520, 906, 1007, 1126, 1294
BstMAI	GTCTC	4 cut(s)	255, 698, 1232, 1576
BstMBI	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
BstMWI	GCNNNNNNNGC	9 cut(s)	490, 1195, 1409, 1412, 1475, 1511, 1517, 1544, 1553
BstNI	CCWGG	4 cut(s)	172, 526, 558, 1613
BstNSI	RCATGY	5 cut(s)	363, 378, 734, 1704, 1708
BstSCI	CCNGG	4 cut(s)	170, 524, 556, 1611
BstSFI	CTRYAG	2 cut(s)	1059, 1548
BstUI	CGCG	1 cut(s)	198
BstX2I	RGATCY	2 cut(s)	903, 1004
BstXI	CCANNNNNNTGG	1 cut(s)	152
BstYI	RGATCY	2 cut(s)	903, 1004
Bsu15I	ATCGAT	1 cut(s)	1089
BsuI	GTATCC	1 cut(s)	198
BsuRI	GGCC	3 cut(s)	138, 333, 888
BsuTUI	ATCGAT	1 cut(s)	1089
BtgZI	GCGATG	1 cut(s)	60
BtsCI	GGATG	4 cut(s)	77, 630, 838, 1130
BtsIMutI	CAGTG	2 cut(s)	652, 1126
BtuMI	TCGCGA	1 cut(s)	198
Cac8I	GCNNGC	5 cut(s)	136, 1029, 1386, 1702, 1706
CaiI	CAGNNNCTG	2 cut(s)	785, 1556
CfoI	GCGC	1 cut(s)	1456
Cfr13I	GGNCC	2 cut(s)	136, 331
ClaI	ATCGAT	1 cut(s)	1089
Csp6I	GTAC	5 cut(s)	12, 164, 350, 728, 1372
CviAII	CATG	7 cut(s)	360, 375, 610, 731, 1701, 1705, 1711
CviQI	GTAC	5 cut(s)	12, 164, 350, 728, 1372
DdeI	CTNAG	8 cut(s)	105, 187, 479, 546, 753, 1407, 1539, 1586
DpnI	GATC	6 cut(s)	93, 519, 905, 1006, 1125, 1293
DpnII	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
Eam1104I	CTCTTC	3 cut(s)	798, 1317, 1574
EarI	CTCTTC	3 cut(s)	798, 1317, 1574
Eco31I	GGTCTC	2 cut(s)	698, 1232
Eco32I	GATATC	1 cut(s)	1423
Eco57I	CTGAAG	3 cut(s)	1269, 1485, 1536
EcoNI	CCTNNNNNAGG	1 cut(s)	815
EcoRII	CCWGG	4 cut(s)	170, 524, 556, 1611
EcoRV	GATATC	1 cut(s)	1423
EcoT22I	ATGCAT	2 cut(s)	1643, 1706
FaeI	CATG	7 cut(s)	363, 378, 613, 734, 1704, 1708, 1714
FatI	CATG	7 cut(s)	359, 374, 609, 730, 1700, 1704, 1710
FauI	CCCGC	1 cut(s)	127
FbaI	TGATCA	1 cut(s)	1123
FblI	GTMKAC	1 cut(s)	55
FokI	GGATG	4 cut(s)	84, 637, 845, 1117
FspBI	CTAG	2 cut(s)	1502, 1506
FspI	TGCGCA	1 cut(s)	1455
GlaI	GCGC	1 cut(s)	1455
GsaI	CCCAGC	1 cut(s)	449
GsuI	CTGGAG	4 cut(s)	154, 1316, 1518, 1634
HaeIII	GGCC	3 cut(s)	138, 333, 888
HapII	CCGG	2 cut(s)	69, 1242
HhaI	GCGC	1 cut(s)	1456
Hin1II	CATG	7 cut(s)	363, 378, 613, 734, 1704, 1708, 1714
Hin6I	GCGC	1 cut(s)	1454
HinP1I	GCGC	1 cut(s)	1454
HincII	GTYRAC	1 cut(s)	1303
HindII	GTYRAC	1 cut(s)	1303
HindIII	AAGCTT	1 cut(s)	1251
HinfI	GANTC	5 cut(s)	782, 1079, 1265, 1363, 1584
HpaII	CCGG	2 cut(s)	69, 1242
Hpy166II	GTNNAC	9 cut(s)	12, 56, 554, 650, 896, 914, 992, 1303, 1561
Hpy188I	TCNGA	7 cut(s)	106, 343, 480, 712, 1270, 1282, 1542
Hpy188III	TCNNGA	8 cut(s)	69, 197, 380, 814, 901, 1295, 1367, 1433
Hpy8I	GTNNAC	9 cut(s)	12, 56, 554, 650, 896, 914, 992, 1303, 1561
HpyAV	CCTTC	7 cut(s)	29, 531, 558, 811, 1322, 1387, 1430
HpyCH4III	ACNGT	2 cut(s)	692, 1121
HpyF10VI	GCNNNNNNNGC	9 cut(s)	490, 1195, 1409, 1412, 1475, 1511, 1517, 1544, 1553
HpyF3I	CTNAG	8 cut(s)	105, 187, 479, 546, 753, 1407, 1539, 1586
Hsp92II	CATG	7 cut(s)	363, 378, 613, 734, 1704, 1708, 1714
HspAI	GCGC	1 cut(s)	1454
Kpn2I	TCCGGA	1 cut(s)	68
Ksp22I	TGATCA	1 cut(s)	1123
Kzo9I	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
LmnI	GCTCC	6 cut(s)	173, 449, 707, 789, 1195, 1381
LweI	GCATC	8 cut(s)	37, 62, 615, 730, 1064, 1095, 1650, 1717
MaeI	CTAG	2 cut(s)	1502, 1506
MaeIII	GTNAC	1 cut(s)	1744
MalI	GATC	6 cut(s)	93, 519, 905, 1006, 1125, 1293
MbiI	CCGCTC	1 cut(s)	316
MboI	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
MfeI	CAATTG	3 cut(s)	146, 498, 1426
MflI	RGATCY	2 cut(s)	903, 1004
MhlI	GDGCHC	2 cut(s)	495, 704
MlyI	GAGTC	3 cut(s)	1073, 1274, 1578
Mph1103I	ATGCAT	2 cut(s)	1643, 1706
MroI	TCCGGA	1 cut(s)	68
MroXI	GAANNNNTTC	1 cut(s)	1622
MseI	TTAA	5 cut(s)	123, 839, 1068, 1317, 1667
MslI	CAYNNNNRTG	3 cut(s)	608, 735, 1709
MspA1I	CMGCKG	3 cut(s)	1232, 1472, 1514
MspI	CCGG	2 cut(s)	69, 1242
MspR9I	CCNGG	4 cut(s)	172, 526, 558, 1613
MunI	CAATTG	3 cut(s)	146, 498, 1426
Mva1269I	GAATGC	1 cut(s)	133
MvaI	CCWGG	4 cut(s)	172, 526, 558, 1613
MvnI	CGCG	1 cut(s)	198
MwoI	GCNNNNNNNGC	9 cut(s)	490, 1195, 1409, 1412, 1475, 1511, 1517, 1544, 1553
NdeII	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
NlaIII	CATG	7 cut(s)	363, 378, 613, 734, 1704, 1708, 1714
NlaIV	GGNNCC	4 cut(s)	40, 111, 332, 1197
NruI	TCGCGA	1 cut(s)	198
NsbI	TGCGCA	1 cut(s)	1455
NsiI	ATGCAT	2 cut(s)	1643, 1706
NspI	RCATGY	5 cut(s)	363, 378, 734, 1704, 1708
NspV	TTCGAA	2 cut(s)	416, 1626
PaeI	GCATGC	2 cut(s)	1704, 1708
PciI	ACATGT	1 cut(s)	730
PcsI	WCGNNNNNNNCGW	1 cut(s)	216
PctI	GAATGC	1 cut(s)	133
PdmI	GAANNNNTTC	1 cut(s)	1622
PfeI	GAWTC	2 cut(s)	782, 1363
PflFI	GACNNNGTC	1 cut(s)	951
PfoI	TCCNGGA	1 cut(s)	1611
PleI	GAGTC	3 cut(s)	1073, 1273, 1578
PpsI	GAGTC	3 cut(s)	1073, 1273, 1578
PscI	ACATGT	1 cut(s)	730
Psp6I	CCWGG	4 cut(s)	170, 524, 556, 1611
PspFI	CCCAGC	1 cut(s)	445
PspGI	CCWGG	4 cut(s)	170, 524, 556, 1611
PspN4I	GGNNCC	4 cut(s)	40, 111, 332, 1197
PspPI	GGNCC	2 cut(s)	136, 331
PstI	CTGCAG	2 cut(s)	1063, 1552
PstNI	CAGNNNCTG	2 cut(s)	785, 1556
PsuI	RGATCY	2 cut(s)	903, 1004
PsyI	GACNNNGTC	1 cut(s)	951
PvuII	CAGCTG	3 cut(s)	1232, 1472, 1514
RruI	TCGCGA	1 cut(s)	198
RsaI	GTAC	5 cut(s)	13, 165, 351, 729, 1373
RsaNI	GTAC	5 cut(s)	12, 164, 350, 728, 1372
RseI	CAYNNNNRTG	3 cut(s)	608, 735, 1709
SaqAI	TTAA	5 cut(s)	123, 839, 1068, 1317, 1667
Sau3AI	GATC	6 cut(s)	91, 517, 903, 1004, 1123, 1291
Sau96I	GGNCC	2 cut(s)	136, 331
SchI	GAGTC	3 cut(s)	1073, 1274, 1578
ScrFI	CCNGG	4 cut(s)	172, 526, 558, 1613
SduI	GDGCHC	2 cut(s)	495, 704
SfaNI	GCATC	8 cut(s)	37, 62, 615, 730, 1064, 1095, 1650, 1717
SfcI	CTRYAG	2 cut(s)	1059, 1548
SfuI	TTCGAA	2 cut(s)	416, 1626
SmiMI	CAYNNNNRTG	3 cut(s)	608, 735, 1709
SmlI	CTYRAG	2 cut(s)	695, 899
SmoI	CTYRAG	2 cut(s)	695, 899
SphI	GCATGC	2 cut(s)	1704, 1708
SsiI	CCGC	5 cut(s)	75, 131, 134, 316, 757
SspMI	CTAG	2 cut(s)	1502, 1506
StyD4I	CCNGG	4 cut(s)	170, 524, 556, 1611
TaaI	ACNGT	2 cut(s)	692, 1121
TaqI	TCGA	4 cut(s)	210, 416, 1089, 1626
TatI	WGTACW	4 cut(s)	11, 349, 727, 1371
TauI	GCSGC	3 cut(s)	78, 134, 760
TfiI	GAWTC	2 cut(s)	782, 1363
Tru1I	TTAA	5 cut(s)	123, 839, 1068, 1317, 1667
Tru9I	TTAA	5 cut(s)	123, 839, 1068, 1317, 1667
TscAI	CASTG	2 cut(s)	652, 1126
TspDTI	ATGAA	8 cut(s)	240, 524, 626, 849, 1335, 1376, 1679, 1752
TspGWI	ACGGA	1 cut(s)	1014
TspRI	CASTG	2 cut(s)	652, 1126
Tth111I	GACNNNGTC	1 cut(s)	951
XagI	CCTNNNNNAGG	1 cut(s)	815
XapI	RAATTY	2 cut(s)	319, 767
XceI	RCATGY	5 cut(s)	363, 378, 734, 1704, 1708
XmiI	GTMKAC	1 cut(s)	55
XmnI	GAANNNNTTC	1 cut(s)	1622
XspI	CTAG	2 cut(s)	1502, 1506
Zsp2I	ATGCAT	2 cut(s)	1643, 1706

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.