Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0000772

Physical Location & Seq

Genomic Coordinates Reverse (-)

3808779 .. 3818571

Gene Structure 9793 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1033900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1986 bp

ATGGTTTTTTGCTCTCACTGTAGAAAGCCAGTTTCTGGAATTAGCGATGGTGGTAGAATTTCGTGTCCCGGATGTGGAAAGGTTTTGGAGTACGAACAATACAGTGAGGAAACCACATTCGTTAAAAATGCAGCTGGACAGAGCCAAGTGGCTGGAAGATTTGTACGGACCATTCAAAGTGATATCTCAGCTTCTCGCGAAAGGATCTTCGAGAATGCCGAATATGAATTAAGATGTATGAGAAATGCTTTGGATATGGGAGACAATGAAGAAATTGTTGACATAGCTAAGCGTTTTTATAGAATTGCAGTAGAGAAAAATTTCACCAGGGGGCGTAAATCAGAGCAAGTACTGGCTTCATGTCTTTACATTGCATGCCGGGAAAAGAGGAGGCCGTATCTTCTTATTGATTTTTCGAACTACTTGAAGATAAATGTTTATGTGCTAGGTGCTGTGTTTTTGCAACTTTGCAAAACTCTTAGGCTCAATGAGCACCCAATTGTCCAAAAACCTGTTGATCCTTCTTGGTTTATTCATAAATTTACAGAGAGTTTACCAGGAGGAACGAATAAAAGGGTCATGCAAACTGCACTGCGGATTATCACTAGCATGAAACGGGATTGGATGCAGACGGGCAGAAAACCCAGTGGGCTATGTGGTGCTGCGCTATATATATCTGGACATTCACATGGTCTCAATTATTCCAAGTCTGATATTACTAGGATTGTGCATGTTTGTGATGCAACATTGACCAAGCGGCTGGTTGAGTTTGAGAATACTGAATCCGGAAGCTTGACGATTGAGGAATTCCTTGAAAAGGCCAGGGAGCTGGAAGAATTCAGCAAAGAACCAATTTTGGCATTAAAAGTGGGTAATGCAGATGATGTCCTTTGTGAGCATAAGGGTAGTGGAAAACCATTTGCATATGGACTTTGTAGAATTTGTTACGATGATTTTATGCAAATTTCAGGTGGACTTGAGGGAGGGTCTAATCCTCCAGCTTTCCAGCGTGCCGAAGTAAAGAGATCGGAGGAGGCATCTGCTGAGCAAATTGCTACGGATTCTGGTGTTGAACCTTGCCAAAGCCCGGACAACAGTAAACAGTTGCCAAATTTTGAGAAAGAACTGAACAAATCAAAAACAGAAAGCAAAGACGAAAGAAAGCAGTTTACAGAGCCCGAAAGTACCACTGTTGATGCGGATGCTGAAGATTGTGATAAGCCTGACAACCTCTCGGATATCGAGGACGCAGAGGTTGATAGCTACCTTCTAAATGAAGAGGGGAAACGTTATAAACAGATGATTTGGGAAGAAATGAACCGGGAGTACATTGAGGAGCAAGCAGAAAAGGAGAGACAAAACAAGGCTGCTGGTATGGATCATGACATTCCAGAAGATGCAAAGAACCTTGGGCAGGCTACAGCGGCAGCTATGGCAAAGAAAAGAAAGGAAAGGCAACTAGCTCGAGAAGCCGAAGCAAGGAGTTCTACTCCAGCTAAAAATGCTGCAGAAGCTACCCACCAGATGTTGACTAAGAAGAGACTCAGCTCAAAAATCAATTTTGAAGCCATTGAGCATATATTCCAAACAGATGCGTTGAAGCTCCAGATGGCTCCAAGAAGAAAAAACTTGAATCTCATCTTGACACTGACAAAGCGGACGACAATGTTGATGAGTTGGGTCCAGAAAATGAATATGAGGACGAGGAGGGAATCGAAGGAGCATTTGGCTACAATCTTGATGATGAAAATGTTGATGAACAACCATACGACGATGAAGATTATGGTTATGATGGCTACTGATGGTGCTTTCTGTCAGCGTCTTATTGAAGCCCATTGGAAGCTAGATTCAAGTGTTTCCATCTATCAAAATCGATATGGTTTGCTCGGTCACACTCCGAACACATTTCTGCAGCCTTCCCCTAAGATTTGTTTTCTTACTGAAAGTTGGATACTGGACACTTTTAGTTTATTTTTTGTAATGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000126 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005667 GO:0006139 GO:0006351 GO:0006352 GO:0006383 GO:0006384 GO:0006399 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009058 GO:0009059 GO:0009303 GO:0009304 GO:0009987 GO:0010467 GO:0016070 GO:0016072 GO:0018130 GO:0019438 GO:0031974 GO:0031981 GO:0032774 GO:0032991 GO:0034641 GO:0034645 GO:0034654 GO:0034660 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044249 GO:0044260 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044798 GO:0046483 GO:0070013 GO:0071704 GO:0090304 GO:0090576 GO:0097659 GO:0098781 GO:1901360 GO:1901362 GO:1901576

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

BRF1 TFIIB

Protein Analysis

662

Amino Acids

75.26

Weight (kDa)

7.18

Isoelectric Point (pI)

48.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TFIIB	PF00382	70 - 158	1.1e-15	Transcription factor TFIIB repeat
TFIIB	PF00382	169 - 259	5.9e-15	Transcription factor TFIIB repeat
BRF1	PF07741	412 - 529	6.9e-23	Brf1-like TBP-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000772)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G30455 AT2G01280 AT2G01280 AT2G01280 AT2G45100 AT2G45100 AT2G45100 AT3G09360 AT3G09360 AT3G09360
fragaria_vesca	FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_4g11810 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_5g32090 FvH4_7g06241
malus_domestica	MD04G1033900.v1.1
prunus_persica	Prupe.1G180700_v2.0.a1
pyrus_communis	pycom04g02830
rosa_chinensis	RchiOBHm_Chr4g0409121 RchiOBHm_Chr7g0228131
rosa_laevigata	RLG00000001586 RLG00000008590
rosa_multiflora	Rmu_sc0001348.1_g000036 Rmu_sc0001348.1_g000042 Rmu_sc0001526.1_g000006 Rmu_sc0001526.1_g000008 Rmu_sc0012031.1_g000013 Rmu_sc0021826.1_g000001 Rmu_ssc0000372.1_g000072
rosa_roxburghii	Rroxscaffold_2G00115130 Rroxscaffold_3G00231370 Rroxscaffold_5G00353680 Rroxscaffold_7G00191300
rosa_rugosa	Rorug04G0090500 Rorug04G0090600 Rorug04G0090700 Rorug07G0245700 Rorug07G0245800
rosa_samantha	Rh2AG319000 Rh4AG154400 Rh4BG152200 Rh4CG163600 Rh4CG264400 Rh4DG147400 Rh5DG375300 Rh7AG400000 Rh7BG381300 Rh7CG418200 Rh7DG396100
rosa_wichuraiana	Rw0G005090 Rw0G011060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1293
AccB7I	CCANNNNNTGG	1 cut(s)	35
AccII	CGCG	1 cut(s)	198
AccIII	TCCGGA	1 cut(s)	785
AciI	CCGC	5 cut(s)	595, 757, 1199, 1424, 1657
AclI	AACGTT	1 cut(s)	1288
AclWI	GGATC	3 cut(s)	212, 512, 1386
AcsI	RAATTY	8 cut(s)	57, 319, 539, 806, 836, 939, 963, 1111
AcuI	CTGAAG	1 cut(s)	1227
AfaI	GTAC	5 cut(s)	92, 165, 351, 1186, 1328
AfiI	CCNNNNNNNGG	6 cut(s)	35, 74, 817, 1087, 1414, 1479
AgsI	TTSAA	9 cut(s)	176, 427, 815, 1073, 1565, 1600, 1633, 1829, 1851
AjnI	CCWGG	3 cut(s)	326, 556, 821
AjuI	GAANNNNNNNTTGG	2 cut(s)	1709, 1741
Alw21I	GWGCWC	1 cut(s)	495
Alw26I	GTCTC	4 cut(s)	255, 698, 1348, 1534
AlwI	GGATC	3 cut(s)	212, 512, 1386
AlwNI	CAGNNNCTG	1 cut(s)	35
Ama87I	CYCGRG	1 cut(s)	1464
Aor13HI	TCCGGA	1 cut(s)	785
AoxI	GGCC	2 cut(s)	392, 819
ApeKI	GCWGC	6 cut(s)	131, 662, 1367, 1427, 1505, 1912
ApoI	RAATTY	8 cut(s)	57, 319, 539, 806, 836, 939, 963, 1111
Asp700I	GAANNNNTTC	1 cut(s)	320
AspLEI	GCGC	1 cut(s)	667
AspS9I	GGNCC	2 cut(s)	168, 1681
AsuC2I	CCSGG	4 cut(s)	69, 380, 1088, 1322
AsuHPI	GGTGA	1 cut(s)	316
AsuII	TTCGAA	1 cut(s)	416
AvaI	CYCGRG	1 cut(s)	1464
AvaII	GGWCC	2 cut(s)	168, 1681
BanII	GRGCYC	1 cut(s)	1179
Bbv12I	GWGCWC	1 cut(s)	495
BbvI	GCAGC	6 cut(s)	143, 649, 1354, 1439, 1492, 1924
BccI	CCATC	5 cut(s)	41, 1603, 1786, 1796, 1868
BceAI	ACGGC	1 cut(s)	379
BcgI	CGANNNNNNTGC	2 cut(s)	1445, 1479
BciT130I	CCWGG	3 cut(s)	328, 558, 823
BciVI	GTATCC	1 cut(s)	1944
BcnI	CCSGG	4 cut(s)	69, 380, 1088, 1322
BcoDI	GTCTC	4 cut(s)	255, 698, 1348, 1534
BfaI	CTAG	5 cut(s)	446, 606, 720, 1460, 1844
BfmI	CTRYAG	4 cut(s)	19, 1419, 1506, 1910
BfuI	GTATCC	1 cut(s)	1944
BisI	GCNGC	8 cut(s)	132, 663, 758, 1368, 1425, 1428, 1506, 1913
BlpI	GCTNAGC	2 cut(s)	288, 1044
BlsI	GCNGC	8 cut(s)	133, 664, 759, 1369, 1426, 1429, 1507, 1914
BmcAI	AGTACT	1 cut(s)	351
Bme1390I	CCNGG	7 cut(s)	69, 328, 380, 558, 823, 1088, 1322
Bme18I	GGWCC	2 cut(s)	168, 1681
BmeT110I	CYCGRG	1 cut(s)	1464
BmgT120I	GGNCC	2 cut(s)	168, 1681
BmiI	GGNNCC	2 cut(s)	1614, 1682
BmrFI	CCNGG	7 cut(s)	69, 328, 380, 558, 823, 1088, 1322
BmrI	ACTGGG	1 cut(s)	639
BmsI	GCATC	7 cut(s)	615, 730, 1046, 1186, 1192, 1387, 1582
BmuI	ACTGGG	1 cut(s)	639
BplI	GAGNNNNNCTC	2 cut(s)	1474, 1506
BpmI	CTGGAG	3 cut(s)	981, 1476, 1589
Bpu1102I	GCTNAGC	2 cut(s)	288, 1044
Bpu14I	TTCGAA	1 cut(s)	416
BpuEI	CTTGAG	1 cut(s)	998
BpuMI	CCSGG	4 cut(s)	69, 380, 1088, 1322
Bsa29I	ATCGAT	1 cut(s)	1873
BsaI	GGTCTC	1 cut(s)	698
BsaJI	CCNNGG	3 cut(s)	327, 822, 1408
BsaWI	WCCGGW	1 cut(s)	785
Bsc4I	CCNNNNNNNGG	6 cut(s)	35, 74, 817, 1087, 1414, 1479
Bse1I	ACTGG	4 cut(s)	29, 357, 645, 1959
Bse3DI	GCAATG	1 cut(s)	369
BseAI	TCCGGA	1 cut(s)	785
BseBI	CCWGG	3 cut(s)	328, 558, 823
BseCI	ATCGAT	1 cut(s)	1873
BseDI	CCNNGG	3 cut(s)	327, 822, 1408
BseGI	GGATG	3 cut(s)	77, 630, 1207
BseLI	CCNNNNNNNGG	6 cut(s)	35, 74, 817, 1087, 1414, 1479
BseMI	GCAATG	1 cut(s)	369
BseMII	CTCAG	3 cut(s)	201, 1035, 1558
BseNI	ACTGG	4 cut(s)	29, 357, 645, 1959
BseRI	GAGGAG	4 cut(s)	403, 1046, 1349, 1720
BseXI	GCAGC	6 cut(s)	143, 649, 1354, 1439, 1492, 1924
BsgI	GTGCAG	1 cut(s)	573
Bsh1236I	CGCG	1 cut(s)	198
BshFI	GGCC	2 cut(s)	394, 821
BshVI	ATCGAT	1 cut(s)	1873
BsiHKAI	GWGCWC	1 cut(s)	495
BsiHKCI	CYCGRG	1 cut(s)	1464
BsiSI	CCGG	5 cut(s)	69, 379, 786, 1088, 1321
BslFI	GGGAC	1 cut(s)	51
BslI	CCNNNNNNNGG	6 cut(s)	35, 74, 817, 1087, 1414, 1479
BsmAI	GTCTC	4 cut(s)	255, 698, 1348, 1534
BsmFI	GGGAC	1 cut(s)	51
BsmI	GAATGC	1 cut(s)	220
BsnI	GGCC	2 cut(s)	394, 821
Bso31I	GGTCTC	1 cut(s)	698
BsoBI	CYCGRG	1 cut(s)	1464
Bsp119I	TTCGAA	1 cut(s)	416
Bsp1286I	GDGCHC	2 cut(s)	495, 1179
Bsp13I	TCCGGA	1 cut(s)	785
Bsp143I	GATC	4 cut(s)	204, 517, 1025, 1378
Bsp1720I	GCTNAGC	2 cut(s)	288, 1044
Bsp68I	TCGCGA	1 cut(s)	198
BspACI	CCGC	5 cut(s)	595, 757, 1199, 1424, 1657
BspANI	GGCC	2 cut(s)	394, 821
BspCNI	CTCAG	3 cut(s)	200, 1036, 1557
BspDI	ATCGAT	1 cut(s)	1873
BspEI	TCCGGA	1 cut(s)	785
BspFNI	CGCG	1 cut(s)	198
BspHI	TCATGA	1 cut(s)	1381
BspLI	GGNNCC	2 cut(s)	1614, 1682
BspMAI	CTGCAG	2 cut(s)	1510, 1914
BspPI	GGATC	3 cut(s)	212, 512, 1386
BspT104I	TTCGAA	1 cut(s)	416
BspTNI	GGTCTC	1 cut(s)	698
BsrDI	GCAATG	1 cut(s)	369
BsrI	ACTGG	4 cut(s)	29, 357, 645, 1959
BssECI	CCNNGG	3 cut(s)	327, 822, 1408
BssMI	GATC	4 cut(s)	204, 517, 1025, 1378
BssT1I	CCWWGG	1 cut(s)	1408
Bst2UI	CCWGG	3 cut(s)	328, 558, 823
Bst4CI	ACNGT	5 cut(s)	20, 104, 1097, 1104, 1192
Bst6I	CTCTTC	2 cut(s)	1272, 1532
BstBI	TTCGAA	1 cut(s)	416
BstC8I	GCNNGC	4 cut(s)	376, 1011, 1341, 1416
BstDEI	CTNAG	7 cut(s)	187, 288, 479, 1044, 1533, 1544, 1923
BstENI	CCTNNNNNAGG	2 cut(s)	815, 1412
BstF5I	GGATG	3 cut(s)	77, 630, 1207
BstFNI	CGCG	1 cut(s)	198
BstHHI	GCGC	1 cut(s)	667
BstKTI	GATC	4 cut(s)	207, 520, 1028, 1381
BstMAI	GTCTC	4 cut(s)	255, 698, 1348, 1534
BstMBI	GATC	4 cut(s)	204, 517, 1025, 1378
BstMWI	GCNNNNNNNGC	6 cut(s)	490, 1424, 1433, 1469, 1502, 1511
BstNI	CCWGG	3 cut(s)	328, 558, 823
BstNSI	RCATGY	2 cut(s)	378, 734
BstSCI	CCNGG	7 cut(s)	67, 326, 378, 556, 821, 1086, 1320
BstSFI	CTRYAG	4 cut(s)	19, 1419, 1506, 1910
BstUI	CGCG	1 cut(s)	198
BstV1I	GCAGC	6 cut(s)	143, 649, 1354, 1439, 1492, 1924
BstX2I	RGATCY	1 cut(s)	204
BstXI	CCANNNNNNTGG	3 cut(s)	152, 760, 829
BstYI	RGATCY	1 cut(s)	204
Bsu15I	ATCGAT	1 cut(s)	1873
BsuI	GTATCC	1 cut(s)	1944
BsuRI	GGCC	2 cut(s)	394, 821
BsuTUI	ATCGAT	1 cut(s)	1873
BtgZI	GCGATG	1 cut(s)	60
BtsCI	GGATG	3 cut(s)	77, 630, 1207
BtsI	GCAGTG	1 cut(s)	590
BtsIMutI	CAGTG	6 cut(s)	16, 109, 590, 652, 1188, 1646
BtuMI	TCGCGA	1 cut(s)	198
Cac8I	GCNNGC	4 cut(s)	376, 1011, 1341, 1416
CaiI	CAGNNNCTG	1 cut(s)	35
CciI	TCATGA	1 cut(s)	1381
CfoI	GCGC	1 cut(s)	667
Cfr13I	GGNCC	2 cut(s)	168, 1681
ClaI	ATCGAT	1 cut(s)	1873
CseI	GACGC	2 cut(s)	1256, 1808
Csp6I	GTAC	5 cut(s)	91, 164, 350, 1185, 1327
CviAII	CATG	7 cut(s)	360, 375, 580, 610, 689, 731, 1382
CviQI	GTAC	5 cut(s)	91, 164, 350, 1185, 1327
DdeI	CTNAG	7 cut(s)	187, 288, 479, 1044, 1533, 1544, 1923
DpnI	GATC	4 cut(s)	206, 519, 1027, 1380
DpnII	GATC	4 cut(s)	204, 517, 1025, 1378
Eam1104I	CTCTTC	2 cut(s)	1272, 1532
EarI	CTCTTC	2 cut(s)	1272, 1532
Eco130I	CCWWGG	1 cut(s)	1408
Eco24I	GRGCYC	1 cut(s)	1179
Eco31I	GGTCTC	1 cut(s)	698
Eco32I	GATATC	2 cut(s)	184, 1240
Eco47I	GGWCC	2 cut(s)	168, 1681
Eco57I	CTGAAG	1 cut(s)	1227
Eco88I	CYCGRG	1 cut(s)	1464
EcoNI	CCTNNNNNAGG	2 cut(s)	815, 1412
EcoRI	GAATTC	2 cut(s)	806, 836
EcoRII	CCWGG	3 cut(s)	326, 556, 821
EcoRV	GATATC	2 cut(s)	184, 1240
EcoT14I	CCWWGG	1 cut(s)	1408
EcoT38I	GRGCYC	1 cut(s)	1179
ErhI	CCWWGG	1 cut(s)	1408
FaeI	CATG	7 cut(s)	363, 378, 583, 613, 692, 734, 1385
FaqI	GGGAC	1 cut(s)	51
FatI	CATG	7 cut(s)	359, 374, 579, 609, 688, 730, 1381
FauNDI	CATATG	1 cut(s)	925
Fnu4HI	GCNGC	8 cut(s)	132, 663, 758, 1368, 1425, 1428, 1506, 1913
FokI	GGATG	3 cut(s)	84, 637, 1214
FriOI	GRGCYC	1 cut(s)	1179
Fsp4HI	GCNGC	8 cut(s)	132, 663, 758, 1368, 1425, 1428, 1506, 1913
FspBI	CTAG	5 cut(s)	446, 606, 720, 1460, 1844
GlaI	GCGC	1 cut(s)	666
GluI	GCNGC	8 cut(s)	132, 663, 758, 1368, 1425, 1428, 1506, 1913
GsuI	CTGGAG	3 cut(s)	981, 1476, 1589
HaeIII	GGCC	2 cut(s)	394, 821
HapII	CCGG	5 cut(s)	69, 379, 786, 1088, 1321
HgaI	GACGC	2 cut(s)	1256, 1808
HhaI	GCGC	1 cut(s)	667
Hin1II	CATG	7 cut(s)	363, 378, 583, 613, 692, 734, 1385
Hin6I	GCGC	1 cut(s)	665
HinP1I	GCGC	1 cut(s)	665
HincII	GTYRAC	2 cut(s)	280, 1530
HindII	GTYRAC	2 cut(s)	280, 1530
HindIII	AAGCTT	1 cut(s)	790
HinfI	GANTC	6 cut(s)	782, 1061, 1542, 1633, 1712, 1847
HpaII	CCGG	5 cut(s)	69, 379, 786, 1088, 1321
HphI	GGTGA	1 cut(s)	316
Hpy166II	GTNNAC	6 cut(s)	280, 554, 974, 1100, 1170, 1530
Hpy188I	TCNGA	5 cut(s)	343, 712, 1030, 1237, 1899
Hpy8I	GTNNAC	6 cut(s)	280, 554, 974, 1100, 1170, 1530
Hpy99I	CGWCG	1 cut(s)	1774
HpyAV	CCTTC	4 cut(s)	531, 1277, 1711, 1926
HpyCH4III	ACNGT	5 cut(s)	20, 104, 1097, 1104, 1192
HpyCH4IV	ACGT	1 cut(s)	1288
HpyF10VI	GCNNNNNNNGC	6 cut(s)	490, 1424, 1433, 1469, 1502, 1511
HpyF3I	CTNAG	7 cut(s)	187, 288, 479, 1044, 1533, 1544, 1923
HpySE526I	ACGT	1 cut(s)	1288
Hsp92II	CATG	7 cut(s)	363, 378, 583, 613, 692, 734, 1385
HspAI	GCGC	1 cut(s)	665
Kpn2I	TCCGGA	1 cut(s)	785
Kzo9I	GATC	4 cut(s)	204, 517, 1025, 1378
LmnI	GCTCC	5 cut(s)	826, 1336, 1608, 1618, 1720
Lsp1109I	GCAGC	6 cut(s)	143, 649, 1354, 1439, 1492, 1924
LweI	GCATC	7 cut(s)	615, 730, 1046, 1186, 1192, 1387, 1582
MaeI	CTAG	5 cut(s)	446, 606, 720, 1460, 1844
MaeII	ACGT	1 cut(s)	1288
MaeIII	GTNAC	2 cut(s)	944, 1889
MalI	GATC	4 cut(s)	206, 519, 1027, 1380
MboI	GATC	4 cut(s)	204, 517, 1025, 1378
MfeI	CAATTG	1 cut(s)	498
MflI	RGATCY	1 cut(s)	204
MhlI	GDGCHC	2 cut(s)	495, 1179
MlyI	GAGTC	1 cut(s)	1536
MmeI	TCCRAC	1 cut(s)	1928
MroI	TCCGGA	1 cut(s)	785
MroXI	GAANNNNTTC	1 cut(s)	320
MseI	TTAA	3 cut(s)	123, 230, 863
MslI	CAYNNNNRTG	3 cut(s)	608, 687, 735
MspA1I	CMGCKG	2 cut(s)	134, 1424
MspI	CCGG	5 cut(s)	69, 379, 786, 1088, 1321
MspR9I	CCNGG	7 cut(s)	69, 328, 380, 558, 823, 1088, 1322
MunI	CAATTG	1 cut(s)	498
Mva1269I	GAATGC	1 cut(s)	220
MvaI	CCWGG	3 cut(s)	328, 558, 823
MvnI	CGCG	1 cut(s)	198
MwoI	GCNNNNNNNGC	6 cut(s)	490, 1424, 1433, 1469, 1502, 1511
NciI	CCSGG	4 cut(s)	69, 380, 1088, 1322
NdeI	CATATG	1 cut(s)	925
NdeII	GATC	4 cut(s)	204, 517, 1025, 1378
NlaIII	CATG	7 cut(s)	363, 378, 583, 613, 692, 734, 1385
NlaIV	GGNNCC	2 cut(s)	1614, 1682
NmuCI	GTSAC	1 cut(s)	1889
NruI	TCGCGA	1 cut(s)	198
NspI	RCATGY	2 cut(s)	378, 734
NspV	TTCGAA	1 cut(s)	416
PaeI	GCATGC	1 cut(s)	378
PaeR7I	CTCGAG	1 cut(s)	1464
PagI	TCATGA	1 cut(s)	1381
PcsI	WCGNNNNNNNCGW	1 cut(s)	216
PctI	GAATGC	1 cut(s)	220
PdmI	GAANNNNTTC	1 cut(s)	320
PfeI	GAWTC	5 cut(s)	782, 1061, 1633, 1712, 1847
PflMI	CCANNNNNTGG	1 cut(s)	35
PfoI	TCCNGGA	1 cut(s)	67
PkrI	GCNGC	8 cut(s)	133, 664, 759, 1369, 1426, 1429, 1507, 1914
PleI	GAGTC	1 cut(s)	1536
PpsI	GAGTC	1 cut(s)	1536
PsiI	TTATAA	1 cut(s)	1293
Psp1406I	AACGTT	1 cut(s)	1288
Psp6I	CCWGG	3 cut(s)	326, 556, 821
PspGI	CCWGG	3 cut(s)	326, 556, 821
PspN4I	GGNNCC	2 cut(s)	1614, 1682
PspPI	GGNCC	2 cut(s)	168, 1681
PsrI	GAACNNNNNNTAC	2 cut(s)	1310, 1342
PstI	CTGCAG	2 cut(s)	1510, 1914
PstNI	CAGNNNCTG	1 cut(s)	35
PsuI	RGATCY	1 cut(s)	204
PvuII	CAGCTG	1 cut(s)	134
RruI	TCGCGA	1 cut(s)	198
RsaI	GTAC	5 cut(s)	92, 165, 351, 1186, 1328
RsaNI	GTAC	5 cut(s)	91, 164, 350, 1185, 1327
RseI	CAYNNNNRTG	3 cut(s)	608, 687, 735
SaqAI	TTAA	3 cut(s)	123, 230, 863
SatI	GCNGC	8 cut(s)	132, 663, 758, 1368, 1425, 1428, 1506, 1913
Sau3AI	GATC	4 cut(s)	204, 517, 1025, 1378
Sau96I	GGNCC	2 cut(s)	168, 1681
ScaI	AGTACT	1 cut(s)	351
SchI	GAGTC	1 cut(s)	1536
ScrFI	CCNGG	7 cut(s)	69, 328, 380, 558, 823, 1088, 1322
SduI	GDGCHC	2 cut(s)	495, 1179
SfaNI	GCATC	7 cut(s)	615, 730, 1046, 1186, 1192, 1387, 1582
SfcI	CTRYAG	4 cut(s)	19, 1419, 1506, 1910
Sfr274I	CTCGAG	1 cut(s)	1464
SfuI	TTCGAA	1 cut(s)	416
SinI	GGWCC	2 cut(s)	168, 1681
SlaI	CTCGAG	1 cut(s)	1464
SmiMI	CAYNNNNRTG	3 cut(s)	608, 687, 735
SmlI	CTYRAG	2 cut(s)	977, 1464
SmoI	CTYRAG	2 cut(s)	977, 1464
SphI	GCATGC	1 cut(s)	378
SsiI	CCGC	5 cut(s)	595, 757, 1199, 1424, 1657
SspMI	CTAG	5 cut(s)	446, 606, 720, 1460, 1844
StyD4I	CCNGG	7 cut(s)	67, 326, 378, 556, 821, 1086, 1320
StyI	CCWWGG	1 cut(s)	1408
TaaI	ACNGT	5 cut(s)	20, 104, 1097, 1104, 1192
TaiI	ACGT	1 cut(s)	1291
TaqI	TCGA	6 cut(s)	210, 416, 1242, 1465, 1715, 1873
TaqII	GACCGA	1 cut(s)	1877
TatI	WGTACW	2 cut(s)	349, 1326
TauI	GCSGC	2 cut(s)	760, 1427
TfiI	GAWTC	5 cut(s)	782, 1061, 1633, 1712, 1847
Tru1I	TTAA	3 cut(s)	123, 230, 863
Tru9I	TTAA	3 cut(s)	123, 230, 863
TscAI	CASTG	6 cut(s)	23, 109, 597, 652, 1195, 1653
TseFI	GTSAC	1 cut(s)	1889
TseI	GCWGC	6 cut(s)	131, 662, 1367, 1427, 1505, 1912
Tsp45I	GTSAC	1 cut(s)	1889
TspGWI	ACGGA	2 cut(s)	181, 1073
TspRI	CASTG	6 cut(s)	23, 109, 597, 652, 1195, 1653
Van91I	CCANNNNNTGG	1 cut(s)	35
VpaK11BI	GGWCC	2 cut(s)	168, 1681
XagI	CCTNNNNNAGG	2 cut(s)	815, 1412
XapI	RAATTY	8 cut(s)	57, 319, 539, 806, 836, 939, 963, 1111
XceI	RCATGY	2 cut(s)	378, 734
XhoI	CTCGAG	1 cut(s)	1464
XmnI	GAANNNNTTC	1 cut(s)	320
XspI	CTAG	5 cut(s)	446, 606, 720, 1460, 1844
ZrmI	AGTACT	1 cut(s)	351

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.