AT1G50920

Involved in the biogenesis of the 60S ribosomal subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0001573
Physical Location & Seq
Forward (+)
18870059 .. 18872968
2910 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G50920.1

Sequence Viewer

Length: 2016 bp
ATGGTTCAATATAATTTCAAGAGGATCACAGTTGTTCCCAATGGGAAGGAGTTCGTTGACATCATCCTTTCACGGACTCAGCGTCAGACACCAACTGTTGTCCACAAGGGTTACAAGATTAACCGTCTCCGTCAGTTCTACATGAGAAAGGTTAAGTACACTCAGACCAACTTCCATGCGAAGCTCTCTGCAATCATTGACGAGTTTCCTCGCCTTGAACAAATCCATCCTTTCTATGGTGATCTTCTTCATGTGCTTTACAATAAAGATCATTACAAGCTTGCTTTAGGCCAAGTGAACACCGCCAGGAACTTGATTAGCAAAATCTCCAAGGATTATGTGAAGCTACTCAAGTATGGTGATTCTTTGTACCGCTGCAAGTGTCTTAAGGTTGCTGCTCTTGGTCGTATGTGCACTGTTTTGAAGCGAATCACTCCTAGTTTGGCTTATCTCGAGCAGATCAGGCAGCACATGGCTAGGCTTCCTTCCATCGATCCTAACACTCGTACTGTCTTGATTTGTGGTTACCCTAATGTTGGCAAGAGTTCTTTTATGAACAAGGTCACCAGAGCTGATGTTGACGTCCAGCCTTATGCTTTCACCACCAAATCCCTCTTTGTTGGTCATACTGATTACAAGTATCTCAGGTACCAAGTCATTGATACCCCCGGGATTTTGGATAGGCCCTTTGAGGACCGCAACATCATTGAAATGTGCAGTATCACTGCCTTGGCCCATCTTCGAGCTGCTGTTTTGTTCTTTCTCGACATTTCTGGATCATGTGGTTACACTATTGCTCAGCAGGCTGCTCTTTTCCACAGCATCAAGTCCCTCTTTATGAACAAACCCTTGGTCATTGTCTGCAACAAGACTGATTTGATGCCCATGGAAAACATCTCTGAAGAAGATCGGAAGCTGATTGAAGAGATGAAGTCTGAAGCTATGAAGACTGCAATGGGAGCAAGTGAAGAACAAGTGCTTTTGAAGATGAGCACTCTGACAGACGAAGGTGTCATGTCTGTGAAGAATGCCGCATGTGAGAGGCTGTTAGATCAAAGGGTAGAGGCCAAGATGAAATCCAAAAAGATCAACGACCACCTAAACAGGTTCCATGTGGCTATCCCGAAGCCTCGTGATAGCATAGAAAGGCTCCCTTGCATACCTCAGGTTGTTCTGGAGGCTAAAGCTAAGGAAGCAGCTGCAATGGAGAAACGAAAGACTGAGAAAGATTTGGAAGAGGAGAATGGTGGAGCTGGAGTTTATTCTGCTAGTCTAAAGAAGAACTACATCTTGCAACATGATGAATGGAAGGAAGACATTATGCCTGAGATTCTCGATGGTCACAATGTGGCTGACTTCATTGATCCAGACATTTTGCAGAGGCTTGCAGAGTTGGAACGTGAAGAAGGGATAAGGGAGGCTGGGGTTGAAGAAGCTGATATGGAGATGGATATTGAAAAGCTTTCGGATGAGCAGCTGAAGCAGCTTTCTGAGATTAGAAAGAAGAAAGCTATACTCATTAAAAACCACAGGCTCAAGAAGACCGTTGCACAGAACCGATCAACTGTTCCAAGAAAGTTTGATAAGGACAAGAAGTACACAACAAAGAGAATGGGTAGGGAGTTATCAGCTATGGGACTTGATCCGTCTTCTGCAATGGACCGTGCAAGAAGCAAGTCTAGAGGGAGGAAGAGGGATCGATCAGAAGATGCGGGTAATGATGCTATGGACGTTGATGATGAGCAACAGTCGAACAAGAAGCAGCGTGTGAGATCAAAGTCTAGAGCTATGTCGATATCAAGATCTCAGTCGAGGCCTCCTGCACATGAAGTTGTGCCTGGTGAAGGATTCAAAGACTCTACTCAGAAGTTATCGGCCATTAAGATTAGCAATAAATCTCACAAAAAGAGAGACAAGAATGCACGTCGTGGTGAAGCTGACAGAGTTATACCAACGCTTAGACCGAAACATTTGTTTTCAGGCAAGAGAGGGAAAGGAAAAACCGACAGGCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1 NOG1 NOGCT

Protein Analysis

671

Amino Acids

76.85

Weight (kDa)

9.62

Isoelectric Point (pI)

45.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NOG1_N PF17835 6 - 165 1.1e-60 NOG1 N-terminal helical domain
FeoB_N PF02421 170 - 320 6.5e-10 Ferrous iron transport protein B
MMR_HSR1 PF01926 171 - 290 1.5e-17 50S ribosome-binding GTPase
NOG1 PF06858 236 - 292 1.2e-24 Nucleolar GTP-binding protein 1 (NOG1)
NOGCT PF08155 405 - 458 8.9e-28 NOGCT (NUC087) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1010
AatII GACGTC 1 cut(s) 585
Acc65I GGTACC 1 cut(s) 648
AccB1I GGYRCC 1 cut(s) 648
AciI CCGC 5 cut(s) 303, 373, 697, 1032, 1712
AclWI GGATC 6 cut(s) 32, 488, 784, 1358, 1637, 1704
AcoI YGGCCR 1 cut(s) 1875
AcuI CTGAAG 3 cut(s) 921, 957, 1499
AcyI GRCGYC 1 cut(s) 582
AdeI CACNNNGTG 2 cut(s) 1348, 1928
AfaI GTAC 5 cut(s) 158, 371, 508, 650, 1598
AfiI CCNNNNNNNGG 3 cut(s) 236, 536, 1844
AflII CTTAAG 1 cut(s) 386
AhdI GACNNNNNGTC 1 cut(s) 81
AjiI CACGTC 1 cut(s) 1925
AjnI CCWGG 2 cut(s) 305, 1837
AjuI GAANNNNNNNTTGG 2 cut(s) 833, 865
Alw21I GWGCWC 2 cut(s) 416, 995
Alw26I GTCTC 2 cut(s) 131, 1905
Alw44I GTGCAC 1 cut(s) 412
AlwI GGATC 6 cut(s) 32, 488, 784, 1358, 1637, 1704
Ama87I CYCGRG 2 cut(s) 452, 668
AoxI GGCC 6 cut(s) 289, 683, 732, 1065, 1814, 1875
ApaLI GTGCAC 1 cut(s) 412
ArsI GACNNNNNNTTYG 2 cut(s) 837, 869
Asp700I GAANNNNTTC 1 cut(s) 50
Asp718I GGTACC 1 cut(s) 648
AspS9I GGNCC 4 cut(s) 684, 694, 733, 1660
AsuC2I CCSGG 2 cut(s) 669, 670
AsuHPI GGTGA 6 cut(s) 251, 371, 556, 592, 1853, 1943
AvaI CYCGRG 2 cut(s) 452, 668
AvaII GGWCC 2 cut(s) 694, 1660
AxyI CCTNAGG 1 cut(s) 1164
BaeGI GKGCMC 1 cut(s) 416
BanI GGYRCC 1 cut(s) 648
BauI CACGAG 1 cut(s) 1131
BbsI GAAGAC 4 cut(s) 953, 1320, 1547, 1641
Bbv12I GWGCWC 2 cut(s) 416, 995
BccI CCATC 5 cut(s) 234, 497, 744, 1331, 1441
BciT130I CCWGG 2 cut(s) 307, 1839
BcnI CCSGG 2 cut(s) 669, 670
BcoDI GTCTC 2 cut(s) 131, 1905
BfaI CTAG 5 cut(s) 438, 477, 1269, 1680, 1782
BfrI CTTAAG 1 cut(s) 386
BglII AGATCT 1 cut(s) 1802
BlpI GCTNAGC 1 cut(s) 798
Bme1390I CCNGG 4 cut(s) 307, 669, 670, 1839
Bme18I GGWCC 2 cut(s) 694, 1660
BmeRI GACNNNNNGTC 1 cut(s) 81
BmeT110I CYCGRG 2 cut(s) 452, 668
BmgBI CACGTC 1 cut(s) 1925
BmgT120I GGNCC 4 cut(s) 684, 694, 733, 1660
BmiI GGNNCC 3 cut(s) 650, 1109, 1151
BmrFI CCNGG 4 cut(s) 307, 669, 670, 1839
BmsI GCATC 4 cut(s) 831, 870, 1699, 1711
BpiI GAAGAC 4 cut(s) 953, 1320, 1547, 1641
BpmI CTGGAG 2 cut(s) 1196, 1275
Bpu10I CCTNAGC 1 cut(s) 1188
Bpu1102I GCTNAGC 1 cut(s) 798
BpuEI CTTGAG 2 cut(s) 335, 1520
BpuMI CCSGG 2 cut(s) 669, 670
Bsa29I ATCGAT 2 cut(s) 492, 1699
BsaHI GRCGYC 1 cut(s) 582
BsaJI CCNNGG 6 cut(s) 330, 667, 668, 729, 849, 885
BsaXI ACNNNNNCTCC 2 cut(s) 1242, 1272
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 536, 1844
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 3 cut(s) 960, 1209, 1662
BseBI CCWGG 2 cut(s) 307, 1839
BseCI ATCGAT 2 cut(s) 492, 1699
BseDI CCNNGG 6 cut(s) 330, 667, 668, 729, 849, 885
BseGI GGATG 3 cut(s) 63, 226, 1474
BseLI CCNNNNNNNGG 3 cut(s) 236, 536, 1844
BseMI GCAATG 3 cut(s) 960, 1209, 1662
BseRI GAGGAG 1 cut(s) 1253
BseSI GKGCMC 1 cut(s) 416
BseYI CCCAGC 1 cut(s) 1421
BsgI GTGCAG 2 cut(s) 736, 1806
BshFI GGCC 6 cut(s) 291, 685, 734, 1067, 1816, 1877
BshNI GGYRCC 1 cut(s) 648
BshVI ATCGAT 2 cut(s) 492, 1699
BsiHKAI GWGCWC 2 cut(s) 416, 995
BsiHKCI CYCGRG 2 cut(s) 452, 668
BsiSI CCGG 1 cut(s) 669
BslFI GGGAC 2 cut(s) 814, 1650
BslI CCNNNNNNNGG 3 cut(s) 236, 536, 1844
BsmAI GTCTC 2 cut(s) 131, 1905
BsmBI CGTCTC 1 cut(s) 131
BsmFI GGGAC 2 cut(s) 814, 1650
BsmI GAATGC 2 cut(s) 1033, 1924
BsnI GGCC 6 cut(s) 291, 685, 734, 1067, 1816, 1877
BsoBI CYCGRG 2 cut(s) 452, 668
Bsp1286I GDGCHC 2 cut(s) 416, 995
Bsp1720I GCTNAGC 1 cut(s) 798
Bsp19I CCATGG 1 cut(s) 885
BspACI CCGC 5 cut(s) 303, 373, 697, 1032, 1712
BspANI GGCC 6 cut(s) 291, 685, 734, 1067, 1816, 1877
BspDI ATCGAT 2 cut(s) 492, 1699
BspLI GGNNCC 3 cut(s) 650, 1109, 1151
BspPI GGATC 6 cut(s) 32, 488, 784, 1358, 1637, 1704
BspT107I GGYRCC 1 cut(s) 648
BspTI CTTAAG 1 cut(s) 386
BsrDI GCAATG 3 cut(s) 960, 1209, 1662
BssECI CCNNGG 6 cut(s) 330, 667, 668, 729, 849, 885
BssNI GRCGYC 1 cut(s) 582
BssSI CACGAG 1 cut(s) 1131
BssT1I CCWWGG 4 cut(s) 330, 729, 849, 885
Bst2BI CACGAG 1 cut(s) 1131
Bst2UI CCWGG 2 cut(s) 307, 1839
Bst4CI ACNGT 9 cut(s) 31, 97, 125, 418, 511, 1546, 1567, 1664, 1749
Bst6I CTCTTC 3 cut(s) 918, 1230, 1685
BstACI GRCGYC 1 cut(s) 582
BstAFI CTTAAG 1 cut(s) 386
BstC8I GCNNGC 3 cut(s) 282, 804, 1386
BstDSI CCRYGG 1 cut(s) 885
BstEII GGTNACC 2 cut(s) 524, 562
BstENI CCTNNNNNAGG 1 cut(s) 1842
BstF5I GGATG 3 cut(s) 63, 226, 1474
BstMAI GTCTC 2 cut(s) 131, 1905
BstMWI GCNNNNNNNGC 6 cut(s) 463, 803, 959, 1193, 1480, 1483
BstNI CCWGG 2 cut(s) 307, 1839
BstNSI RCATGY 1 cut(s) 1038
BstPI GGTNACC 2 cut(s) 524, 562
BstSCI CCNGG 4 cut(s) 305, 667, 668, 1837
BstSLI GKGCMC 1 cut(s) 416
BstV2I GAAGAC 4 cut(s) 953, 1320, 1547, 1641
BstX2I RGATCY 1 cut(s) 1802
BstYI RGATCY 1 cut(s) 1802
Bsu15I ATCGAT 2 cut(s) 492, 1699
Bsu36I CCTNAGG 1 cut(s) 1164
BsuRI GGCC 6 cut(s) 291, 685, 734, 1067, 1816, 1877
BsuTUI ATCGAT 2 cut(s) 492, 1699
BtgI CCRYGG 1 cut(s) 885
BtrI CACGTC 1 cut(s) 1925
BtsCI GGATG 3 cut(s) 63, 226, 1474
BtsI GCAGTG 1 cut(s) 723
BtsIMutI CAGTG 2 cut(s) 414, 723
Cac8I GCNNGC 3 cut(s) 282, 804, 1386
Cfr13I GGNCC 4 cut(s) 684, 694, 733, 1660
Cfr9I CCCGGG 1 cut(s) 668
ClaI ATCGAT 2 cut(s) 492, 1699
CseI GACGC 1 cut(s) 71
Csp6I GTAC 5 cut(s) 157, 370, 507, 649, 1597
CviQI GTAC 5 cut(s) 157, 370, 507, 649, 1597
DraIII CACNNNGTG 2 cut(s) 1348, 1928
DrdI GACNNNNNNGTC 1 cut(s) 1010
DriI GACNNNNNGTC 1 cut(s) 81
DseDI GACNNNNNNGTC 1 cut(s) 1010
EaeI YGGCCR 1 cut(s) 1875
Eam1104I CTCTTC 3 cut(s) 918, 1230, 1685
Eam1105I GACNNNNNGTC 1 cut(s) 81
EarI CTCTTC 3 cut(s) 918, 1230, 1685
Eco130I CCWWGG 4 cut(s) 330, 729, 849, 885
Eco147I AGGCCT 1 cut(s) 1816
Eco32I GATATC 1 cut(s) 1797
Eco47I GGWCC 2 cut(s) 694, 1660
Eco57I CTGAAG 3 cut(s) 921, 957, 1499
Eco81I CCTNAGG 1 cut(s) 1164
Eco88I CYCGRG 2 cut(s) 452, 668
Eco91I GGTNACC 2 cut(s) 524, 562
EcoNI CCTNNNNNAGG 1 cut(s) 1842
EcoO109I RGGNCCY 1 cut(s) 684
EcoO65I GGTNACC 2 cut(s) 524, 562
EcoRII CCWGG 2 cut(s) 305, 1837
EcoRV GATATC 1 cut(s) 1797
EcoT14I CCWWGG 4 cut(s) 330, 729, 849, 885
ErhI CCWWGG 4 cut(s) 330, 729, 849, 885
Esp3I CGTCTC 1 cut(s) 131
FalI AAGNNNNNCTT 4 cut(s) 818, 850, 1138, 1170
FaqI GGGAC 2 cut(s) 814, 1650
FauI CCCGC 1 cut(s) 1705
FokI GGATG 3 cut(s) 50, 213, 1481
FspBI CTAG 5 cut(s) 438, 477, 1269, 1680, 1782
GsaI CCCAGC 1 cut(s) 1425
GsuI CTGGAG 2 cut(s) 1196, 1275
HaeIII GGCC 6 cut(s) 291, 685, 734, 1067, 1816, 1877
HapII CCGG 1 cut(s) 669
HgaI GACGC 1 cut(s) 71
Hin1I GRCGYC 1 cut(s) 582
HincII GTYRAC 2 cut(s) 58, 580
HindII GTYRAC 2 cut(s) 58, 580
HindIII AAGCTT 2 cut(s) 278, 1460
HinfI GANTC 6 cut(s) 76, 362, 429, 1330, 1848, 1856
HpaII CCGG 1 cut(s) 669
HphI GGTGA 6 cut(s) 251, 371, 556, 592, 1853, 1943
Hpy166II GTNNAC 7 cut(s) 58, 103, 159, 298, 414, 580, 1599
Hpy8I GTNNAC 7 cut(s) 58, 103, 159, 298, 414, 580, 1599
Hpy99I CGWCG 1 cut(s) 1929
HpyAV CCTTC 6 cut(s) 40, 495, 1001, 1303, 1400, 1838
HpyCH4III ACNGT 9 cut(s) 31, 97, 125, 418, 511, 1546, 1567, 1664, 1749
HpyCH4IV ACGT 4 cut(s) 582, 1399, 1731, 1924
HpyF10VI GCNNNNNNNGC 6 cut(s) 463, 803, 959, 1193, 1480, 1483
HpySE526I ACGT 4 cut(s) 582, 1399, 1731, 1924
Hsp92I GRCGYC 1 cut(s) 582
KpnI GGTACC 1 cut(s) 652
LmnI GCTCC 3 cut(s) 959, 1155, 1250
LweI GCATC 4 cut(s) 831, 870, 1699, 1711
MaeI CTAG 5 cut(s) 438, 477, 1269, 1680, 1782
MaeII ACGT 4 cut(s) 582, 1399, 1731, 1924
MaeIII GTNAC 5 cut(s) 110, 524, 562, 785, 1340
MflI RGATCY 1 cut(s) 1802
MhlI GDGCHC 2 cut(s) 416, 995
MluCI AATT 1 cut(s) 13
MlyI GAGTC 2 cut(s) 70, 1850
MmeI TCCRAC 1 cut(s) 1374
MroXI GAANNNNTTC 1 cut(s) 50
MseI TTAA 5 cut(s) 120, 153, 387, 1521, 1881
MslI CAYNNNNRTG 2 cut(s) 710, 1019
MspA1I CMGCKG 3 cut(s) 375, 1199, 1477
MspCI CTTAAG 1 cut(s) 386
MspI CCGG 1 cut(s) 669
MspR9I CCNGG 4 cut(s) 307, 669, 670, 1839
Mva1269I GAATGC 2 cut(s) 1033, 1924
MvaI CCWGG 2 cut(s) 307, 1839
MwoI GCNNNNNNNGC 6 cut(s) 463, 803, 959, 1193, 1480, 1483
NciI CCSGG 2 cut(s) 669, 670
NcoI CCATGG 1 cut(s) 885
NlaIV GGNNCC 3 cut(s) 650, 1109, 1151
NmuCI GTSAC 2 cut(s) 562, 1340
NspI RCATGY 1 cut(s) 1038
PaeR7I CTCGAG 1 cut(s) 452
PceI AGGCCT 1 cut(s) 1816
PcsI WCGNNNNNNNCGW 2 cut(s) 79, 1961
PctI GAATGC 2 cut(s) 1033, 1924
PdmI GAANNNNTTC 1 cut(s) 50
PfeI GAWTC 4 cut(s) 362, 429, 1330, 1848
PleI GAGTC 2 cut(s) 70, 1850
PpsI GAGTC 2 cut(s) 70, 1850
Psp6I CCWGG 2 cut(s) 305, 1837
PspEI GGTNACC 2 cut(s) 524, 562
PspFI CCCAGC 1 cut(s) 1421
PspGI CCWGG 2 cut(s) 305, 1837
PspN4I GGNNCC 3 cut(s) 650, 1109, 1151
PspPI GGNCC 4 cut(s) 684, 694, 733, 1660
PsuI RGATCY 1 cut(s) 1802
PvuII CAGCTG 2 cut(s) 1199, 1477
RsaI GTAC 5 cut(s) 158, 371, 508, 650, 1598
RsaNI GTAC 5 cut(s) 157, 370, 507, 649, 1597
RseI CAYNNNNRTG 2 cut(s) 710, 1019
SaqAI TTAA 5 cut(s) 120, 153, 387, 1521, 1881
Sau96I GGNCC 4 cut(s) 684, 694, 733, 1660
SchI GAGTC 2 cut(s) 70, 1850
ScrFI CCNGG 4 cut(s) 307, 669, 670, 1839
SduI GDGCHC 2 cut(s) 416, 995
SfaNI GCATC 4 cut(s) 831, 870, 1699, 1711
Sfr274I CTCGAG 1 cut(s) 452
SinI GGWCC 2 cut(s) 694, 1660
SlaI CTCGAG 1 cut(s) 452
SmaI CCCGGG 1 cut(s) 670
SmiMI CAYNNNNRTG 2 cut(s) 710, 1019
SmlI CTYRAG 4 cut(s) 350, 386, 452, 1535
SmoI CTYRAG 4 cut(s) 350, 386, 452, 1535
Sse9I AATT 1 cut(s) 13
SseBI AGGCCT 1 cut(s) 1816
SsiI CCGC 5 cut(s) 303, 373, 697, 1032, 1712
SspMI CTAG 5 cut(s) 438, 477, 1269, 1680, 1782
StuI AGGCCT 1 cut(s) 1816
StyD4I CCNGG 4 cut(s) 305, 667, 668, 1837
StyI CCWWGG 4 cut(s) 330, 729, 849, 885
TaaI ACNGT 9 cut(s) 31, 97, 125, 418, 511, 1546, 1567, 1664, 1749
TaiI ACGT 4 cut(s) 585, 1402, 1734, 1927
TaqI TCGA 9 cut(s) 453, 492, 742, 765, 1335, 1699, 1751, 1793, 1811
TaqII GACCGA 1 cut(s) 1978
TasI AATT 1 cut(s) 13
TatI WGTACW 2 cut(s) 156, 1596
TauI GCSGC 1 cut(s) 1034
TfiI GAWTC 4 cut(s) 362, 429, 1330, 1848
Tru1I TTAA 5 cut(s) 120, 153, 387, 1521, 1881
Tru9I TTAA 5 cut(s) 120, 153, 387, 1521, 1881
TscAI CASTG 2 cut(s) 421, 730
TseFI GTSAC 2 cut(s) 562, 1340
Tsp45I GTSAC 2 cut(s) 562, 1340
TspDTI ATGAA 9 cut(s) 239, 569, 854, 944, 959, 1088, 1317, 1348, 1842
TspGWI ACGGA 3 cut(s) 88, 119, 1635
TspMI CCCGGG 1 cut(s) 668
TspRI CASTG 2 cut(s) 421, 730
Vha464I CTTAAG 1 cut(s) 386
VneI GTGCAC 1 cut(s) 412
VpaK11BI GGWCC 2 cut(s) 694, 1660
XagI CCTNNNNNAGG 1 cut(s) 1842
XbaI TCTAGA 2 cut(s) 1679, 1781
XceI RCATGY 1 cut(s) 1038
XcmI CCANNNNNNNNNTGG 1 cut(s) 233
XhoI CTCGAG 1 cut(s) 452
XmaI CCCGGG 1 cut(s) 668
XmnI GAANNNNTTC 1 cut(s) 50
XspI CTAG 5 cut(s) 438, 477, 1269, 1680, 1782
ZraI GACGTC 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.