Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0001573

Physical Location & Seq

Genomic Coordinates Forward (+)

37567092 .. 37571024

Gene Structure 3933 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1277600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGACTGTTGTACCAAATGGGAAGGACTTCATTGACATTATCCTGTCTCGCACTCAGAGGCAGACCCCAACTGTAGTCCACAAGGGTTATGCCATTTCCCGCCTTCGTCAGTTCTATATGCGCAAAGTCAAATATACTCAACAGAATTTTCATGAAAAGCTCTCCACAATAGTTGATGAGTTTCCTAGGCTGGATGACATCCACCCTTTTTATGGCGACCTCCTTCATGTTCTCTACAACAAAGACCACTACAAGCTTGCACTTGGCCAAATCAATACTGCAAGAAATCTTATTGGAAAGATTTCTAAGGATTATGTGAAGTTGTTGAAGTATGGTGATTCACTTTACCGATGCAAATGTCTCAAGGTCGCTGCTCTTGGCCGCATGTGCACGGTTATAAAGAGGATCGGTCCTAGTTTAGCTTACCTGGAACAAATTAGGCAACACATGGCAAGGCTACCTTCAATTGACCCTAATACCAGGACAATATTGATTTGTGGGTATCCGAATGTTGGAAAGAGCTCATTCATTAACAAGATCACAAGGGCTGATGTGGATGTCCAGCCCTATGCCTTTACCACCAAGTCACTCTTTGTGGGGCATACAGATTATAAATACCTGAGGTACCAGGTAATTGATACACCAGGGATCTTGGACCGACCATTTGAAGACCGTAACATTATTGAGATGTGCAGCATCACAGCTTTGGCGCACTTGAGAGCTGCGGTGTTGTTCTTCTTGGACATCTCAGGGTCTTGTGGGTACAGCATTGCCCAACAAGCAGCTCTTTTTCATAGCATCAAGTCTCTGTTTATGAACAAACCATTGATTATAGTCTGCAACAAGACTGATTTGCAGCCATTGGATGGTATATCCGAGGAAGACAAGAAGTTGGTCGCAGAGATGAAATCTGAAGCTATGAAGACAGTGATCGGTCAGGGAGGTGAGGCTACAAATGATGATGGGGTACTCTTGACCATGAGTACTTTGACCGAGGAGGGGGTAATTGCTGTAAAGAATGCAGCTTGTGACAGGTTGTTGAATCAGAGAGTTGAGCTGAAGATGAAATCCAAAAAGATAAATGATTGCTTAAATCGTTTTCATGTTGCCATGCCAAAGCCTCGTGACAACAAGGAAAGGCCCCCATGCATACCTCAGGCAGTTATAGAAGCTAAGATTAAGCAAGCAGCGGAGAAGGAAAAGAGACAGACAGAAAGGGATTTGGAGAATGAGAATGGCGGTGCAGGTGTATACTCTGCCAGTTTGAAGAAGCATTATATCTTAGCTAATGATGAATGGAAAGAGGATGTCATGCCAGAAATTCTTGATGGGCACAATGTGTATGACTTTGTTGATCCTGATATTTTACATAGGCTTGAGGAGTTGGAACGAGAAGAAGGACTCCGTCAGGAAGAGGAGGGTGATGATTTTGAGATGGATGGATTGGAATTGACTCCAGAAGAGAAAAAGACATTGGCTGCGATCAGGAAAAAGAAAAGCTTGCTCATTCAAGAACATAGAATCAAGAAAAGCACAGCAGAGAGTCGACCCATTGTACCAAGGAAATTTGACAAGGATCGGGAATTCACAACAAAGAGAATGGGGAGGCAACTATCAAAATTAGGAATTGATCCTAGTAGGGCAATTGAACGGGCCCGAAGCAGATCTGTGTCTAGGGGACGGAAGAGAGAGAGATCAGTTGACAGGGGTGATACTGATGGTGGCGATGCTATGGAAGTGGATACACCCAACAAGAAGCAGCGTATGTTATCTAGATCCCGTTCAAGGTCCAGGTCAAGGCCTCCGACTGAAGTCGTCCCTGGAGAGGGCTTCAAGGACTCTAATCAGAAGGCTAAGGCACTACAGAAGGCCAGGAAATCTAATAAGAATAGAAACAAGGATGCTCGTCGTGGAGAGGCAGATAGGGTCATTCCCACTCTAAAACCAAAGCACTTGTTCTCTGGAAAGCGCTCAATAGGAAAAACCCAGAGGCGTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0016020

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

MMR_HSR1 NOG1 NOGCT

Protein Analysis

666

Amino Acids

75.88

Weight (kDa)

9.58

Isoelectric Point (pI)

46.73

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
NOG1_N	PF17835	1 - 157	6.8e-60	NOG1 N-terminal helical domain
FeoB_N	PF02421	162 - 311	9.2e-10	Ferrous iron transport protein B
MMR_HSR1	PF01926	163 - 282	3.7e-18	50S ribosome-binding GTPase
NOG1	PF06858	228 - 284	3.9e-25	Nucleolar GTP-binding protein 1 (NOG1)
NOGCT	PF08155	402 - 455	2.9e-28	NOGCT (NUC087) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0001573)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G10300 AT1G10300 AT1G10300 AT1G50920
fragaria_vesca	FvH4_3g28930 FvH4_3g28930 FvH4_6g37740
malus_domestica	MD06G1031200.v1.1 MD11G1222700.v1.1 MD16G1277600.v1.1
prunus_persica	Prupe.1G572400_v2.0.a1 Prupe.1G572400_v2.0.a1 Prupe.1G572400_v2.0.a1 Prupe.4G247400_v2.0.a1 Prupe.5G045700_v2.0.a1 Prupe.5G046000_v2.0.a1 Prupe.5G046000_v2.0.a1 Prupe.6G149400_v2.0.a1
pyrus_communis	pycom03g13360 pycom06g02600 pycom11g16960 pycom11g19650
rosa_chinensis	RchiOBHm_Chr3g0491451 RchiOBHm_Chr5g0051831 RchiOBHm_Chr5g0051911
rosa_laevigata	RLG00000034859 RLG00000034861
rosa_multiflora	Rmu_co8440613.1_g000002 Rmu_sc0001267.1_g000040
rosa_roxburghii	Rroxscaffold_1G00027370 Rroxscaffold_1G00027400
rosa_rugosa	Rorug03G0248800 Rorug03G0248800 Rorug03G0249700 Rorug05G0276600 Rorug05G0276700 Rorug05G0276800
rosa_samantha	Rh3AG298700 Rh3DG332500 Rh3DG333200 Rh5AG348600 Rh5AG348900 Rh5BG359100 Rh5BG359200 Rh5BG359900 Rh5CG384700 Rh5CG384900 Rh5DG374600
rosa_wichuraiana	Rw3G026370 Rw5G032900

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	398, 612
AarI	CACCTGC	1 cut(s)	1235
Acc16I	TGCGCA	1 cut(s)	122
Acc36I	ACCTGC	1 cut(s)	1235
Acc65I	GGTACC	1 cut(s)	624
AccB1I	GGYRCC	1 cut(s)	624
AccB7I	CCANNNNNTGG	1 cut(s)	866
AccI	GTMKAC	2 cut(s)	1251, 1546
AciI	CCGC	5 cut(s)	100, 382, 725, 1190, 1239
AclWI	GGATC	6 cut(s)	413, 656, 1349, 1584, 1625, 1770
AcoI	YGGCCR	2 cut(s)	265, 379
AcsI	RAATTY	4 cut(s)	145, 1320, 1565, 1583
AcuI	CTGAAG	3 cut(s)	933, 1079, 1830
AdeI	CACNNNGTG	1 cut(s)	1339
AfaI	GTAC	6 cut(s)	12, 626, 764, 969, 985, 1557
AfeI	AGCGCT	1 cut(s)	1970
AfiI	CCNNNNNNNGG	8 cut(s)	212, 512, 866, 999, 1785, 1797, 1825, 1826
AgsI	TTSAA	9 cut(s)	328, 465, 668, 1042, 1267, 1511, 1649, 1785, 1834
AjnI	CCWGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
AjuI	GAANNNNNNNTTGG	2 cut(s)	1457, 1489
Alw21I	GWGCWC	2 cut(s)	392, 524
Alw26I	GTCTC	4 cut(s)	51, 365, 810, 1198
Alw44I	GTGCAC	1 cut(s)	388
AlwI	GGATC	6 cut(s)	413, 656, 1349, 1584, 1625, 1770
Aor51HI	AGCGCT	1 cut(s)	1970
AoxI	GGCC	6 cut(s)	265, 379, 1139, 1653, 1799, 1869
ApaI	GGGCCC	1 cut(s)	1657
ApaLI	GTGCAC	1 cut(s)	388
ApeKI	GCWGC	9 cut(s)	371, 693, 722, 782, 856, 1022, 1187, 1478, 1759
ApoI	RAATTY	4 cut(s)	145, 1320, 1565, 1583
ArsI	GACNNNNNNTTYG	2 cut(s)	647, 679
Asp700I	GAANNNNTTC	2 cut(s)	26, 301
Asp718I	GGTACC	1 cut(s)	624
AspA2I	CCTAGG	1 cut(s)	185
AspLEI	GCGC	3 cut(s)	123, 712, 1971
AspS9I	GGNCC	6 cut(s)	410, 655, 1140, 1653, 1654, 1788
AsuHPI	GGTGA	4 cut(s)	347, 956, 1433, 1721
AvaII	GGWCC	3 cut(s)	410, 655, 1788
AvrII	CCTAGG	1 cut(s)	185
AxyI	CCTNAGG	2 cut(s)	620, 1155
BaeGI	GKGCMC	3 cut(s)	392, 1335, 1657
BaeI	ACNNNNGTAYC	1 cut(s)	27
BalI	TGGCCA	1 cut(s)	267
BanI	GGYRCC	1 cut(s)	624
BanII	GRGCYC	2 cut(s)	524, 1657
BauI	CACGAG	1 cut(s)	1122
BbsI	GAAGAC	3 cut(s)	675, 888, 929
Bbv12I	GWGCWC	2 cut(s)	392, 524
BbvI	GCAGC	9 cut(s)	358, 705, 709, 794, 868, 1034, 1199, 1465, 1771
BccI	CCATC	6 cut(s)	860, 956, 1322, 1429, 1433, 1712
BciT130I	CCWGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
BciVI	GTATCC	2 cut(s)	513, 1735
BcoDI	GTCTC	4 cut(s)	51, 365, 810, 1198
BfaI	CTAG	5 cut(s)	186, 414, 1635, 1674, 1773
BfmI	CTRYAG	2 cut(s)	72, 1862
BfoI	RGCGCY	1 cut(s)	1972
BfuAI	ACCTGC	1 cut(s)	1235
BfuI	GTATCC	2 cut(s)	513, 1735
BglII	AGATCT	1 cut(s)	1664
BlnI	CCTAGG	1 cut(s)	185
BmcAI	AGTACT	1 cut(s)	985
Bme1390I	CCNGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
Bme18I	GGWCC	3 cut(s)	410, 655, 1788
BmgT120I	GGNCC	6 cut(s)	410, 655, 1140, 1653, 1654, 1788
BmiI	GGNNCC	3 cut(s)	626, 1142, 1655
BmrFI	CCNGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
BmsI	GCATC	5 cut(s)	341, 705, 807, 1717, 1891
BoxI	GACNNNNGTC	1 cut(s)	1811
BpiI	GAAGAC	3 cut(s)	675, 888, 929
BpmI	CTGGAG	2 cut(s)	1440, 1842
Bpu10I	CCTNAGC	1 cut(s)	1854
BpuEI	CTTGAG	3 cut(s)	347, 736, 1397
BsaJI	CCNNGG	6 cut(s)	185, 644, 876, 993, 1559, 1819
Bsc4I	CCNNNNNNNGG	8 cut(s)	212, 512, 866, 999, 1785, 1797, 1825, 1826
Bse1I	ACTGG	1 cut(s)	1260
Bse21I	CCTNAGG	2 cut(s)	620, 1155
Bse3DI	GCAATG	1 cut(s)	768
BseBI	CCWGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
BseDI	CCNNGG	6 cut(s)	185, 644, 876, 993, 1559, 1819
BseGI	GGATG	7 cut(s)	198, 199, 562, 871, 1312, 1444, 1906
BseLI	CCNNNNNNNGG	8 cut(s)	212, 512, 866, 999, 1785, 1797, 1825, 1826
BseMI	GCAATG	1 cut(s)	768
BseMII	CTCAG	4 cut(s)	68, 611, 762, 1169
BseNI	ACTGG	1 cut(s)	1260
BseRI	GAGGAG	3 cut(s)	1010, 1394, 1430
BseSI	GKGCMC	3 cut(s)	392, 1335, 1657
BseXI	GCAGC	9 cut(s)	358, 705, 709, 794, 868, 1034, 1199, 1465, 1771
BsgI	GTGCAG	2 cut(s)	712, 1263
BshFI	GGCC	6 cut(s)	267, 381, 1141, 1655, 1801, 1871
BshNI	GGYRCC	1 cut(s)	624
BsiHKAI	GWGCWC	2 cut(s)	392, 524
BslFI	GGGAC	2 cut(s)	1692, 1802
BslI	CCNNNNNNNGG	8 cut(s)	212, 512, 866, 999, 1785, 1797, 1825, 1826
BsmAI	GTCTC	4 cut(s)	51, 365, 810, 1198
BsmFI	GGGAC	2 cut(s)	1692, 1802
BsmI	GAATGC	1 cut(s)	1024
BsnI	GGCC	6 cut(s)	267, 381, 1141, 1655, 1801, 1871
Bsp120I	GGGCCC	1 cut(s)	1653
Bsp1286I	GDGCHC	4 cut(s)	392, 524, 1335, 1657
BspACI	CCGC	5 cut(s)	100, 382, 725, 1190, 1239
BspANI	GGCC	6 cut(s)	267, 381, 1141, 1655, 1801, 1871
BspCNI	CTCAG	4 cut(s)	67, 612, 761, 1168
BspHI	TCATGA	1 cut(s)	151
BspLI	GGNNCC	3 cut(s)	626, 1142, 1655
BspMI	ACCTGC	1 cut(s)	1235
BspPI	GGATC	6 cut(s)	413, 656, 1349, 1584, 1625, 1770
BspT107I	GGYRCC	1 cut(s)	624
BsrDI	GCAATG	1 cut(s)	768
BsrI	ACTGG	1 cut(s)	1260
BssECI	CCNNGG	6 cut(s)	185, 644, 876, 993, 1559, 1819
BssNAI	GTATAC	1 cut(s)	1252
BssSI	CACGAG	1 cut(s)	1122
BssT1I	CCWWGG	2 cut(s)	185, 1559
Bst1107I	GTATAC	1 cut(s)	1252
Bst2BI	CACGAG	1 cut(s)	1122
Bst2UI	CCWGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
Bst4CI	ACNGT	5 cut(s)	7, 73, 394, 674, 928
Bst6I	CTCTTC	3 cut(s)	1407, 1455, 1679
BstC8I	GCNNGC	3 cut(s)	258, 1185, 1502
BstDEI	CTNAG	8 cut(s)	54, 306, 620, 748, 1155, 1173, 1282, 1854
BstF5I	GGATG	7 cut(s)	198, 199, 562, 871, 1312, 1444, 1906
BstH2I	RGCGCY	1 cut(s)	1972
BstHHI	GCGC	3 cut(s)	123, 712, 1971
BstMAI	GTCTC	4 cut(s)	51, 365, 810, 1198
BstMWI	GCNNNNNNNGC	2 cut(s)	387, 779
BstNI	CCWGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
BstNSI	RCATGY	1 cut(s)	388
BstPAI	GACNNNNGTC	1 cut(s)	1811
BstSCI	CCNGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
BstSFI	CTRYAG	2 cut(s)	72, 1862
BstSLI	GKGCMC	3 cut(s)	392, 1335, 1657
BstV1I	GCAGC	9 cut(s)	358, 705, 709, 794, 868, 1034, 1199, 1465, 1771
BstV2I	GAAGAC	3 cut(s)	675, 888, 929
BstX2I	RGATCY	3 cut(s)	648, 1664, 1775
BstYI	RGATCY	3 cut(s)	648, 1664, 1775
BstZ17I	GTATAC	1 cut(s)	1252
Bsu36I	CCTNAGG	2 cut(s)	620, 1155
BsuI	GTATCC	2 cut(s)	513, 1735
BsuRI	GGCC	6 cut(s)	267, 381, 1141, 1655, 1801, 1871
BtgZI	GCGATG	1 cut(s)	1740
BtsCI	GGATG	7 cut(s)	198, 199, 562, 871, 1312, 1444, 1906
BtsIMutI	CAGTG	1 cut(s)	933
BveI	ACCTGC	1 cut(s)	1235
Cac8I	GCNNGC	3 cut(s)	258, 1185, 1502
CciI	TCATGA	1 cut(s)	151
CfoI	GCGC	3 cut(s)	123, 712, 1971
Cfr13I	GGNCC	6 cut(s)	410, 655, 1140, 1653, 1654, 1788
CsiI	ACCWGGT	1 cut(s)	627
Csp6I	GTAC	6 cut(s)	11, 625, 763, 968, 984, 1556
CspCI	CAANNNNNGTGG	2 cut(s)	154, 189
CviAII	CATG	9 cut(s)	152, 227, 385, 448, 979, 1103, 1111, 1146, 1312
CviQI	GTAC	6 cut(s)	11, 625, 763, 968, 984, 1556
DdeI	CTNAG	8 cut(s)	54, 306, 620, 748, 1155, 1173, 1282, 1854
DraIII	CACNNNGTG	1 cut(s)	1339
EaeI	YGGCCR	2 cut(s)	265, 379
Eam1104I	CTCTTC	3 cut(s)	1407, 1455, 1679
EarI	CTCTTC	3 cut(s)	1407, 1455, 1679
Ecl136II	GAGCTC	1 cut(s)	522
Eco130I	CCWWGG	2 cut(s)	185, 1559
Eco147I	AGGCCT	1 cut(s)	1801
Eco24I	GRGCYC	2 cut(s)	524, 1657
Eco47I	GGWCC	3 cut(s)	410, 655, 1788
Eco47III	AGCGCT	1 cut(s)	1970
Eco53kI	GAGCTC	1 cut(s)	522
Eco57I	CTGAAG	3 cut(s)	933, 1079, 1830
Eco81I	CCTNAGG	2 cut(s)	620, 1155
EcoICRI	GAGCTC	1 cut(s)	522
EcoO109I	RGGNCCY	1 cut(s)	1140
EcoRI	GAATTC	1 cut(s)	1583
EcoRII	CCWGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
EcoT14I	CCWWGG	2 cut(s)	185, 1559
EcoT22I	ATGCAT	1 cut(s)	1151
EcoT38I	GRGCYC	2 cut(s)	524, 1657
ErhI	CCWWGG	2 cut(s)	185, 1559
FaeI	CATG	9 cut(s)	155, 230, 388, 451, 982, 1106, 1114, 1149, 1315
FalI	AAGNNNNNCTT	8 cut(s)	445, 477, 575, 607, 771, 803, 1484, 1516
FaqI	GGGAC	2 cut(s)	1692, 1802
FatI	CATG	9 cut(s)	151, 226, 384, 447, 978, 1102, 1110, 1145, 1311
FauI	CCCGC	1 cut(s)	107
FblI	GTMKAC	2 cut(s)	1251, 1546
FokI	GGATG	7 cut(s)	185, 206, 569, 878, 1319, 1451, 1913
FriOI	GRGCYC	2 cut(s)	524, 1657
FspBI	CTAG	5 cut(s)	186, 414, 1635, 1674, 1773
FspI	TGCGCA	1 cut(s)	122
GlaI	GCGC	3 cut(s)	122, 711, 1970
GsuI	CTGGAG	2 cut(s)	1440, 1842
HaeII	RGCGCY	1 cut(s)	1972
HaeIII	GGCC	6 cut(s)	267, 381, 1141, 1655, 1801, 1871
HhaI	GCGC	3 cut(s)	123, 712, 1971
Hin1II	CATG	9 cut(s)	155, 230, 388, 451, 982, 1106, 1114, 1149, 1315
Hin6I	GCGC	3 cut(s)	121, 710, 1969
HinP1I	GCGC	3 cut(s)	121, 710, 1969
HincII	GTYRAC	2 cut(s)	1547, 1702
HindII	GTYRAC	2 cut(s)	1547, 1702
HindIII	AAGCTT	2 cut(s)	254, 1498
HinfI	GANTC	7 cut(s)	338, 1042, 1401, 1453, 1521, 1543, 1838
HphI	GGTGA	4 cut(s)	347, 956, 1433, 1721
Hpy166II	GTNNAC	5 cut(s)	79, 390, 1252, 1547, 1702
Hpy188I	TCNGA	7 cut(s)	57, 507, 877, 913, 1047, 1806, 1848
Hpy8I	GTNNAC	5 cut(s)	79, 390, 1252, 1547, 1702
Hpy99I	CGWCG	1 cut(s)	1911
HpyAV	CCTTC	8 cut(s)	16, 113, 233, 471, 1189, 1391, 1843, 1861
HpyCH4III	ACNGT	5 cut(s)	7, 73, 394, 674, 928
HpyF10VI	GCNNNNNNNGC	2 cut(s)	387, 779
HpyF3I	CTNAG	8 cut(s)	54, 306, 620, 748, 1155, 1173, 1282, 1854
Hsp92II	CATG	9 cut(s)	155, 230, 388, 451, 982, 1106, 1114, 1149, 1315
HspAI	GCGC	3 cut(s)	121, 710, 1969
KpnI	GGTACC	1 cut(s)	628
Lsp1109I	GCAGC	9 cut(s)	358, 705, 709, 794, 868, 1034, 1199, 1465, 1771
LweI	GCATC	5 cut(s)	341, 705, 807, 1717, 1891
MabI	ACCWGGT	1 cut(s)	627
MaeI	CTAG	5 cut(s)	186, 414, 1635, 1674, 1773
MaeIII	GTNAC	4 cut(s)	585, 674, 1028, 1124
MfeI	CAATTG	2 cut(s)	465, 1644
MflI	RGATCY	3 cut(s)	648, 1664, 1775
MhlI	GDGCHC	4 cut(s)	392, 524, 1335, 1657
MlsI	TGGCCA	1 cut(s)	267
MluNI	TGGCCA	1 cut(s)	267
MlyI	GAGTC	4 cut(s)	1395, 1447, 1552, 1832
MmeI	TCCRAC	3 cut(s)	493, 1365, 1829
Mox20I	TGGCCA	1 cut(s)	267
Mph1103I	ATGCAT	1 cut(s)	1151
MroXI	GAANNNNTTC	2 cut(s)	26, 301
MscI	TGGCCA	1 cut(s)	267
MseI	TTAA	3 cut(s)	531, 1091, 1179
Msp20I	TGGCCA	1 cut(s)	267
MspA1I	CMGCKG	1 cut(s)	1190
MspR9I	CCNGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
MunI	CAATTG	2 cut(s)	465, 1644
Mva1269I	GAATGC	1 cut(s)	1024
MvaI	CCWGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
MwoI	GCNNNNNNNGC	2 cut(s)	387, 779
NlaIII	CATG	9 cut(s)	155, 230, 388, 451, 982, 1106, 1114, 1149, 1315
NlaIV	GGNNCC	3 cut(s)	626, 1142, 1655
NmuCI	GTSAC	3 cut(s)	585, 1028, 1124
NsbI	TGCGCA	1 cut(s)	122
NsiI	ATGCAT	1 cut(s)	1151
NspI	RCATGY	1 cut(s)	388
PagI	TCATGA	1 cut(s)	151
PaqCI	CACCTGC	1 cut(s)	1235
PceI	AGGCCT	1 cut(s)	1801
PctI	GAATGC	1 cut(s)	1024
PdmI	GAANNNNTTC	2 cut(s)	26, 301
PfeI	GAWTC	3 cut(s)	338, 1042, 1521
PflFI	GACNNNGTC	1 cut(s)	1404
PflMI	CCANNNNNTGG	1 cut(s)	866
PleI	GAGTC	4 cut(s)	1395, 1447, 1551, 1832
PpsI	GAGTC	4 cut(s)	1395, 1447, 1551, 1832
PshAI	GACNNNNGTC	1 cut(s)	1811
PsiI	TTATAA	2 cut(s)	398, 612
Psp124BI	GAGCTC	1 cut(s)	524
Psp6I	CCWGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
PspGI	CCWGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
PspN4I	GGNNCC	3 cut(s)	626, 1142, 1655
PspOMI	GGGCCC	1 cut(s)	1653
PspPI	GGNCC	6 cut(s)	410, 655, 1140, 1653, 1654, 1788
PsuI	RGATCY	3 cut(s)	648, 1664, 1775
PsyI	GACNNNGTC	1 cut(s)	1404
RsaI	GTAC	6 cut(s)	12, 626, 764, 969, 985, 1557
RsaNI	GTAC	6 cut(s)	11, 625, 763, 968, 984, 1556
SacI	GAGCTC	1 cut(s)	524
SalI	GTCGAC	1 cut(s)	1545
SaqAI	TTAA	3 cut(s)	531, 1091, 1179
Sau96I	GGNCC	6 cut(s)	410, 655, 1140, 1653, 1654, 1788
ScaI	AGTACT	1 cut(s)	985
SchI	GAGTC	4 cut(s)	1395, 1447, 1552, 1832
ScrFI	CCNGG	7 cut(s)	428, 481, 629, 645, 1792, 1821, 1873
SduI	GDGCHC	4 cut(s)	392, 524, 1335, 1657
SexAI	ACCWGGT	1 cut(s)	627
SfaNI	GCATC	5 cut(s)	341, 705, 807, 1717, 1891
SfcI	CTRYAG	2 cut(s)	72, 1862
SinI	GGWCC	3 cut(s)	410, 655, 1788
SmlI	CTYRAG	3 cut(s)	362, 715, 1376
SmoI	CTYRAG	3 cut(s)	362, 715, 1376
SseBI	AGGCCT	1 cut(s)	1801
SsiI	CCGC	5 cut(s)	100, 382, 725, 1190, 1239
SspI	AATATT	1 cut(s)	489
SspMI	CTAG	5 cut(s)	186, 414, 1635, 1674, 1773
SstI	GAGCTC	1 cut(s)	524
StuI	AGGCCT	1 cut(s)	1801
StyD4I	CCNGG	7 cut(s)	426, 479, 627, 643, 1790, 1819, 1871
StyI	CCWWGG	2 cut(s)	185, 1559
TaaI	ACNGT	5 cut(s)	7, 73, 394, 674, 928
TaqI	TCGA	1 cut(s)	1546
TaqII	GACCGA	4 cut(s)	398, 672, 923, 1007
TatI	WGTACW	1 cut(s)	983
TauI	GCSGC	1 cut(s)	384
TfiI	GAWTC	3 cut(s)	338, 1042, 1521
Tru1I	TTAA	3 cut(s)	531, 1091, 1179
Tru9I	TTAA	3 cut(s)	531, 1091, 1179
TscAI	CASTG	1 cut(s)	933
TseFI	GTSAC	3 cut(s)	585, 1028, 1124
TseI	GCWGC	9 cut(s)	371, 693, 722, 782, 856, 1022, 1187, 1478, 1759
Tsp45I	GTSAC	3 cut(s)	585, 1028, 1124
TspGWI	ACGGA	2 cut(s)	1394, 1696
TspRI	CASTG	1 cut(s)	933
Tth111I	GACNNNGTC	1 cut(s)	1404
Van91I	CCANNNNNTGG	1 cut(s)	866
VneI	GTGCAC	1 cut(s)	388
VpaK11BI	GGWCC	3 cut(s)	410, 655, 1788
XapI	RAATTY	4 cut(s)	145, 1320, 1565, 1583
XbaI	TCTAGA	1 cut(s)	1772
XceI	RCATGY	1 cut(s)	388
XcmI	CCANNNNNNNNNTGG	1 cut(s)	209
XmaJI	CCTAGG	1 cut(s)	185
XmiI	GTMKAC	2 cut(s)	1251, 1546
XmnI	GAANNNNTTC	2 cut(s)	26, 301
XspI	CTAG	5 cut(s)	186, 414, 1635, 1674, 1773
ZrmI	AGTACT	1 cut(s)	985
Zsp2I	ATGCAT	1 cut(s)	1151

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.