AT1G69085

E3 ubiquitin-protein ligase At3g02290-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0002004
Physical Location & Seq
Forward (+)
25974861 .. 25975997
1137 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G69085.1

Sequence Viewer

Length: 354 bp
ATGGGTATTCTACTTTGTTGTTTTCAAATTCCGGAGTACAATGATGAATCTCTAAACGTACCAAGAATCAGAGCCTTCACTAACCCAATAACTAATAACTTTTCCCCCAAGCTCGAGCAAATATTTCAAAGCTTAGAAAGAGAGGAAATTTCCACTGTCGCCGACGAAGAAGACGTTTGTCCGACATGTTTCTATGAGTATATTGATGAAAACCCGAAGATAGTTTTGCAATGTGGACATATCTTTCACCTTGCATGCATCTATGAATGGATGGAGAGAAGTGAAGTTTGTCCCTTTTGTTCCAAGACAATGCTTTTCTTGGAGGGTGAAGAAATTACACAGCAGGTGGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

117

Amino Acids

13.7

Weight (kDa)

4.31

Isoelectric Point (pI)

51.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 57 - 100 1.9e-10 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 58 - 106 1.5e-08 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 59 - 100 8.8e-12 Ring finger domain
zf-RING_5 PF14634 60 - 102 1.8e-08 zinc-RING finger domain
zf-C3HC4 PF00097 60 - 100 9.3e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4_2 PF13923 60 - 100 3.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 60 - 98 1.9e-07 RING-type zinc-finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 334
Acc36I ACCTGC 1 cut(s) 334
AccIII TCCGGA 1 cut(s) 31
AcsI RAATTY 2 cut(s) 27, 147
AfaI GTAC 2 cut(s) 38, 60
AflIII ACRYGT 1 cut(s) 185
AgsI TTSAA 2 cut(s) 26, 128
AluBI AGCT 2 cut(s) 112, 132
AluI AGCT 2 cut(s) 112, 132
Ama87I CYCGRG 1 cut(s) 113
Aor13HI TCCGGA 1 cut(s) 31
ApoI RAATTY 2 cut(s) 27, 147
AsuHPI GGTGA 2 cut(s) 239, 338
AvaI CYCGRG 1 cut(s) 113
BbsI GAAGAC 1 cut(s) 177
BccI CCATC 1 cut(s) 265
BfuAI ACCTGC 1 cut(s) 334
BmeT110I CYCGRG 1 cut(s) 113
BmsI GCATC 1 cut(s) 267
BoxI GACNNNNGTC 1 cut(s) 177
BpiI GAAGAC 1 cut(s) 177
BsaWI WCCGGW 1 cut(s) 31
Bse3DI GCAATG 1 cut(s) 236
BseAI TCCGGA 1 cut(s) 31
BseGI GGATG 1 cut(s) 276
BseMI GCAATG 1 cut(s) 236
BsiHKCI CYCGRG 1 cut(s) 113
BsiSI CCGG 1 cut(s) 32
BslFI GGGAC 1 cut(s) 276
BsmFI GGGAC 1 cut(s) 276
BsoBI CYCGRG 1 cut(s) 113
Bsp13I TCCGGA 1 cut(s) 31
BspEI TCCGGA 1 cut(s) 31
BspMI ACCTGC 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 236
Bst4CI ACNGT 1 cut(s) 157
BstC8I GCNNGC 1 cut(s) 256
BstDEI CTNAG 1 cut(s) 133
BstF5I GGATG 1 cut(s) 276
BstNSI RCATGY 2 cut(s) 189, 258
BstPAI GACNNNNGTC 1 cut(s) 177
BstV2I GAAGAC 1 cut(s) 177
BtsCI GGATG 1 cut(s) 276
BtsIMutI CAGTG 1 cut(s) 153
BveI ACCTGC 1 cut(s) 334
Cac8I GCNNGC 1 cut(s) 256
Csp6I GTAC 2 cut(s) 37, 59
CviAII CATG 2 cut(s) 186, 255
CviJI RGCY 3 cut(s) 74, 112, 132
CviKI_1 RGCY 3 cut(s) 74, 112, 132
CviQI GTAC 2 cut(s) 37, 59
DdeI CTNAG 1 cut(s) 133
Eco88I CYCGRG 1 cut(s) 113
EcoT22I ATGCAT 1 cut(s) 260
FaeI CATG 2 cut(s) 189, 258
FaiI YATR 6 cut(s) 187, 195, 201, 240, 256, 264
FaqI GGGAC 1 cut(s) 276
FatI CATG 2 cut(s) 185, 254
FokI GGATG 1 cut(s) 283
HapII CCGG 1 cut(s) 32
Hin1II CATG 2 cut(s) 189, 258
HindIII AAGCTT 1 cut(s) 130
HinfI GANTC 2 cut(s) 47, 66
HpaII CCGG 1 cut(s) 32
HphI GGTGA 2 cut(s) 239, 338
Hpy166II GTNNAC 1 cut(s) 236
Hpy188I TCNGA 2 cut(s) 71, 183
Hpy188III TCNNGA 1 cut(s) 32
Hpy8I GTNNAC 1 cut(s) 236
Hpy99I CGWCG 1 cut(s) 167
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 1 cut(s) 157
HpyCH4IV ACGT 2 cut(s) 57, 174
HpyCH4V TGCA 3 cut(s) 229, 254, 258
HpyF3I CTNAG 1 cut(s) 133
HpySE526I ACGT 2 cut(s) 57, 174
Hsp92II CATG 2 cut(s) 189, 258
Kpn2I TCCGGA 1 cut(s) 31
LpnPI CCDG 2 cut(s) 45, 329
LweI GCATC 1 cut(s) 267
MaeII ACGT 2 cut(s) 57, 174
MboII GAAGA 4 cut(s) 179, 182, 229, 341
MluCI AATT 3 cut(s) 27, 147, 333
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 2 cut(s) 136, 316
Mph1103I ATGCAT 1 cut(s) 260
MroI TCCGGA 1 cut(s) 31
MspI CCGG 1 cut(s) 32
NlaIII CATG 2 cut(s) 189, 258
NsiI ATGCAT 1 cut(s) 260
NspI RCATGY 2 cut(s) 189, 258
PaeI GCATGC 1 cut(s) 258
PaeR7I CTCGAG 1 cut(s) 113
PaqCI CACCTGC 1 cut(s) 334
PciI ACATGT 1 cut(s) 185
PcsI WCGNNNNNNNCGW 1 cut(s) 171
PfeI GAWTC 2 cut(s) 47, 66
PscI ACATGT 1 cut(s) 185
PshAI GACNNNNGTC 1 cut(s) 177
PspXI VCTCGAGB 1 cut(s) 113
RsaI GTAC 2 cut(s) 38, 60
RsaNI GTAC 2 cut(s) 37, 59
SetI ASST 6 cut(s) 60, 114, 134, 177, 252, 348
SfaNI GCATC 1 cut(s) 267
Sfr274I CTCGAG 1 cut(s) 113
SlaI CTCGAG 1 cut(s) 113
SmlI CTYRAG 1 cut(s) 113
SmoI CTYRAG 1 cut(s) 113
SphI GCATGC 1 cut(s) 258
Sse9I AATT 3 cut(s) 27, 147, 333
SspI AATATT 1 cut(s) 123
TaaI ACNGT 1 cut(s) 157
TaiI ACGT 2 cut(s) 60, 177
TaqI TCGA 1 cut(s) 114
TasI AATT 3 cut(s) 27, 147, 333
TatI WGTACW 1 cut(s) 36
TfiI GAWTC 2 cut(s) 47, 66
TscAI CASTG 1 cut(s) 160
TspDTI ATGAA 3 cut(s) 60, 222, 279
TspRI CASTG 1 cut(s) 160
XapI RAATTY 2 cut(s) 27, 147
XceI RCATGY 2 cut(s) 189, 258
XhoI CTCGAG 1 cut(s) 113
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.