MD16G1142200.v1.1

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0002004
Physical Location & Seq
Reverse (-)
10941875 .. 10944572
2698 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1142200.v1.1.491

Sequence Viewer

Length: 705 bp
ATGGGTTTTCATTGGATGGGTGCTCTTTGCTGCTGTCCTTGTGGTGAGGAATTTGAAGAATACGCGCTTCCGAGCAATTCAATTTATAGGCATTGCTTATGCCTGAGATACTTCTTTCACCAGCTATTCACTGGGTATAGTGCACCTTTTCAAAGGCTTGATGGACGACCACCAACATTACCTGTTCAAGGGGCCACTTTGGCATCAACCGGAGTAGGCACAACGCTACAAAATAATCCCTTGAATGATACTCAGCTCTCAGTTTCCTGGCCAACCCCCTTCGATGCTGATCAGAGATACTCGCGTTTGCAGCGTGATGGCTTGGTCTCTAGGCGTGAGAAGTCAATGACTCATTTGCAAGAAGATACACAACAACTGAGAAGAGGCAGTTCTGGTACCGAATCCCTGGGTTTTGGGAAGAAATGGAATGGAGATGATACTGAAGAAGATTGTAAGTTTGGCCAATCTGAGACCTCAGAAAAGGCTTTGGCAACAAAACTCGCATATGGACTAACTTACGTGCAACCATCTTCTGAAGATGAAGATGTCTGCCCTACATGTCTGGATGAATACACTTCAGAAAATCCAAAAATCACAACGAGATGTTCTCATCATTTTCACCTTGGCTGTATTTATGAATGGTTGGAGAGAAGCGAAAGCTGTCCAATTTGCGGCAAGGAGATGGAGTTCTGCGAAAGCCCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

235

Amino Acids

26.46

Weight (kDa)

5.02

Isoelectric Point (pI)

53.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 181 - 224 4.3e-11 RING-H2 zinc finger domain
zf-RING_2 PF13639 183 - 224 4.3e-12 Ring finger domain
zf-ANAPC11 PF12861 183 - 230 1.2e-06 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-C3HC4_2 PF13923 184 - 224 1.2e-08 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 184 - 222 1.2e-06 RING-type zinc-finger
zf-C3HC4 PF00097 184 - 224 1.8e-06 Zinc finger, C3HC4 type (RING finger)
zf-RING_5 PF14634 184 - 226 6.4e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 395
AccB1I GGYRCC 1 cut(s) 395
AccII CGCG 2 cut(s) 65, 304
AciI CCGC 1 cut(s) 672
AcoI YGGCCR 2 cut(s) 269, 460
AcsI RAATTY 1 cut(s) 50
AcuI CTGAAG 3 cut(s) 462, 555, 561
AfaI GTAC 1 cut(s) 397
AfiI CCNNNNNNNGG 2 cut(s) 188, 671
AflIII ACRYGT 1 cut(s) 557
AgsI TTSAA 5 cut(s) 56, 81, 152, 188, 244
AjnI CCWGG 2 cut(s) 266, 405
AloI GAACNNNNNNTCC 2 cut(s) 671, 703
AluBI AGCT 3 cut(s) 124, 256, 660
AluI AGCT 3 cut(s) 124, 256, 660
Alw21I GWGCWC 2 cut(s) 25, 145
Alw26I GTCTC 2 cut(s) 331, 464
Alw44I GTGCAC 1 cut(s) 141
AoxI GGCC 3 cut(s) 192, 269, 460
ApaLI GTGCAC 1 cut(s) 141
ApeKI GCWGC 2 cut(s) 30, 310
ApoI RAATTY 1 cut(s) 50
Asp718I GGTACC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 1 cut(s) 192
AsuHPI GGTGA 3 cut(s) 56, 110, 611
BaeGI GKGCMC 1 cut(s) 145
BaeI ACNNNNGTAYC 2 cut(s) 289, 322
BalI TGGCCA 2 cut(s) 271, 462
BanI GGYRCC 1 cut(s) 395
Bbv12I GWGCWC 2 cut(s) 25, 145
BbvI GCAGC 2 cut(s) 17, 322
BccI CCATC 5 cut(s) 10, 155, 311, 535, 676
BciT130I CCWGG 2 cut(s) 268, 407
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 2 cut(s) 331, 464
BfaI CTAG 1 cut(s) 330
BglI GCCNNNNNGGC 1 cut(s) 200
BisI GCNGC 3 cut(s) 31, 311, 673
BlsI GCNGC 3 cut(s) 32, 312, 674
Bme1390I CCNGG 2 cut(s) 268, 407
BmgT120I GGNCC 1 cut(s) 192
BmiI GGNNCC 2 cut(s) 193, 397
BmrFI CCNGG 2 cut(s) 268, 407
BmrI ACTGGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 212, 274
BmuI ACTGGG 1 cut(s) 141
BplI GAGNNNNNCTC 2 cut(s) 592, 624
BsaAI YACGTR 1 cut(s) 520
BsaBI GATNNNNATC 1 cut(s) 288
BsaI GGTCTC 2 cut(s) 331, 464
BsaJI CCNNGG 3 cut(s) 405, 406, 622
BsaWI WCCGGW 1 cut(s) 209
BsaXI ACNNNNNCTCC 2 cut(s) 671, 701
Bsc4I CCNNNNNNNGG 2 cut(s) 188, 671
Bse1I ACTGG 1 cut(s) 136
Bse3DI GCAATG 1 cut(s) 91
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 2 cut(s) 268, 407
BseDI CCNNGG 3 cut(s) 405, 406, 622
BseGI GGATG 2 cut(s) 21, 571
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 188, 671
BseMI GCAATG 1 cut(s) 91
BseMII CTCAG 6 cut(s) 95, 266, 273, 368, 459, 489
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 145
BseXI GCAGC 2 cut(s) 17, 322
Bsh1236I CGCG 2 cut(s) 65, 304
BshFI GGCC 3 cut(s) 194, 271, 462
BshNI GGYRCC 1 cut(s) 395
BsiHKAI GWGCWC 2 cut(s) 25, 145
BsiSI CCGG 1 cut(s) 210
BslI CCNNNNNNNGG 2 cut(s) 188, 671
BsmAI GTCTC 2 cut(s) 331, 464
BsnI GGCC 3 cut(s) 194, 271, 462
Bso31I GGTCTC 2 cut(s) 331, 464
Bsp1286I GDGCHC 2 cut(s) 25, 145
Bsp143I GATC 1 cut(s) 289
BspACI CCGC 1 cut(s) 672
BspANI GGCC 3 cut(s) 194, 271, 462
BspCNI CTCAG 6 cut(s) 96, 265, 272, 369, 460, 488
BspFNI CGCG 2 cut(s) 65, 304
BspLI GGNNCC 2 cut(s) 193, 397
BspT107I GGYRCC 1 cut(s) 395
BspTNI GGTCTC 2 cut(s) 331, 464
BsrDI GCAATG 1 cut(s) 91
BsrI ACTGG 1 cut(s) 136
BssECI CCNNGG 3 cut(s) 405, 406, 622
BssMI GATC 1 cut(s) 289
BssT1I CCWWGG 1 cut(s) 622
Bst2UI CCWGG 2 cut(s) 268, 407
Bst6I CTCTTC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 520
BstDEI CTNAG 6 cut(s) 104, 252, 259, 377, 468, 475
BstENI CCTNNNNNAGG 1 cut(s) 186
BstF5I GGATG 2 cut(s) 21, 571
BstFNI CGCG 2 cut(s) 65, 304
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 1 cut(s) 292
BstMAI GTCTC 2 cut(s) 331, 464
BstMBI GATC 1 cut(s) 289
BstMWI GCNNNNNNNGC 2 cut(s) 200, 310
BstNI CCWGG 2 cut(s) 268, 407
BstNSI RCATGY 1 cut(s) 561
BstSCI CCNGG 2 cut(s) 266, 405
BstSLI GKGCMC 1 cut(s) 145
BstUI CGCG 2 cut(s) 65, 304
BstV1I GCAGC 2 cut(s) 17, 322
BsuRI GGCC 3 cut(s) 194, 271, 462
BtsCI GGATG 2 cut(s) 21, 571
BtsIMutI CAGTG 1 cut(s) 129
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 1 cut(s) 192
Csp6I GTAC 1 cut(s) 396
CviAII CATG 1 cut(s) 558
CviQI GTAC 1 cut(s) 396
DdeI CTNAG 6 cut(s) 104, 252, 259, 377, 468, 475
DpnI GATC 1 cut(s) 291
DpnII GATC 1 cut(s) 289
EaeI YGGCCR 2 cut(s) 269, 460
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 622
Eco31I GGTCTC 2 cut(s) 331, 464
Eco57I CTGAAG 3 cut(s) 462, 555, 561
EcoNI CCTNNNNNAGG 1 cut(s) 186
EcoRII CCWGG 2 cut(s) 266, 405
EcoT14I CCWWGG 1 cut(s) 622
ErhI CCWWGG 1 cut(s) 622
FaeI CATG 1 cut(s) 561
FaiI YATR 7 cut(s) 87, 100, 138, 505, 507, 559, 636
FatI CATG 1 cut(s) 557
FauNDI CATATG 1 cut(s) 505
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 31, 311, 673
FokI GGATG 2 cut(s) 28, 578
Fsp4HI GCNGC 3 cut(s) 31, 311, 673
FspBI CTAG 1 cut(s) 330
GlaI GCGC 1 cut(s) 66
GluI GCNGC 3 cut(s) 31, 311, 673
HaeIII GGCC 3 cut(s) 194, 271, 462
HapII CCGG 1 cut(s) 210
HhaI GCGC 1 cut(s) 67
Hin1II CATG 1 cut(s) 561
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HinfI GANTC 2 cut(s) 349, 401
HpaII CCGG 1 cut(s) 210
HphI GGTGA 3 cut(s) 56, 110, 611
Hpy166II GTNNAC 1 cut(s) 143
Hpy188I TCNGA 6 cut(s) 72, 294, 469, 478, 535, 580
Hpy188III TCNNGA 1 cut(s) 563
Hpy8I GTNNAC 1 cut(s) 143
HpyAV CCTTC 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 519
HpyCH4V TGCA 4 cut(s) 143, 310, 358, 523
HpyF10VI GCNNNNNNNGC 2 cut(s) 200, 310
HpyF3I CTNAG 6 cut(s) 104, 252, 259, 377, 468, 475
HpySE526I ACGT 1 cut(s) 519
Hsp92II CATG 1 cut(s) 561
HspAI GCGC 1 cut(s) 65
KpnI GGTACC 1 cut(s) 399
Ksp22I TGATCA 1 cut(s) 289
Kzo9I GATC 1 cut(s) 289
Lsp1109I GCAGC 2 cut(s) 17, 322
LweI GCATC 2 cut(s) 212, 274
MaeI CTAG 1 cut(s) 330
MaeII ACGT 1 cut(s) 519
MalI GATC 1 cut(s) 291
MboI GATC 1 cut(s) 289
MboII GAAGA 9 cut(s) 68, 374, 393, 430, 455, 458, 522, 548, 554
MhlI GDGCHC 2 cut(s) 25, 145
MlsI TGGCCA 2 cut(s) 271, 462
MluCI AATT 4 cut(s) 50, 76, 81, 666
MluNI TGGCCA 2 cut(s) 271, 462
MlyI GAGTC 1 cut(s) 343
MmeI TCCRAC 1 cut(s) 624
MnlI CCTC 3 cut(s) 40, 377, 484
Mox20I TGGCCA 2 cut(s) 271, 462
MscI TGGCCA 2 cut(s) 271, 462
MseI TTAA 1 cut(s) 703
Msp20I TGGCCA 2 cut(s) 271, 462
MspI CCGG 1 cut(s) 210
MspR9I CCNGG 2 cut(s) 268, 407
MvaI CCWGG 2 cut(s) 268, 407
MvnI CGCG 2 cut(s) 65, 304
MwoI GCNNNNNNNGC 2 cut(s) 200, 310
NdeI CATATG 1 cut(s) 505
NdeII GATC 1 cut(s) 289
NlaIII CATG 1 cut(s) 561
NlaIV GGNNCC 2 cut(s) 193, 397
NspI RCATGY 1 cut(s) 561
PasI CCCWGGG 1 cut(s) 406
PciI ACATGT 1 cut(s) 557
PfeI GAWTC 1 cut(s) 401
PkrI GCNGC 3 cut(s) 32, 312, 674
PleI GAGTC 1 cut(s) 343
PpsI GAGTC 1 cut(s) 343
Ppu21I YACGTR 1 cut(s) 520
PscI ACATGT 1 cut(s) 557
Psp6I CCWGG 2 cut(s) 266, 405
PspGI CCWGG 2 cut(s) 266, 405
PspN4I GGNNCC 2 cut(s) 193, 397
PspPI GGNCC 1 cut(s) 192
RsaI GTAC 1 cut(s) 397
RsaNI GTAC 1 cut(s) 396
SaqAI TTAA 1 cut(s) 703
SatI GCNGC 3 cut(s) 31, 311, 673
Sau3AI GATC 1 cut(s) 289
Sau96I GGNCC 1 cut(s) 192
SchI GAGTC 1 cut(s) 343
ScrFI CCNGG 2 cut(s) 268, 407
SduI GDGCHC 2 cut(s) 25, 145
SetI ASST 8 cut(s) 126, 148, 184, 258, 476, 522, 624, 662
SfaNI GCATC 2 cut(s) 212, 274
Sse9I AATT 4 cut(s) 50, 76, 81, 666
SsiI CCGC 1 cut(s) 672
SspMI CTAG 1 cut(s) 330
StyD4I CCNGG 2 cut(s) 266, 405
StyI CCWWGG 1 cut(s) 622
TaiI ACGT 1 cut(s) 522
TaqI TCGA 1 cut(s) 282
TasI AATT 4 cut(s) 50, 76, 81, 666
TauI GCSGC 1 cut(s) 675
TfiI GAWTC 1 cut(s) 401
Tru1I TTAA 1 cut(s) 703
Tru9I TTAA 1 cut(s) 703
TscAI CASTG 1 cut(s) 136
TseI GCWGC 2 cut(s) 30, 310
TspDTI ATGAA 3 cut(s) 555, 582, 651
TspRI CASTG 1 cut(s) 136
VneI GTGCAC 1 cut(s) 141
XagI CCTNNNNNAGG 1 cut(s) 186
XapI RAATTY 1 cut(s) 50
XceI RCATGY 1 cut(s) 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 128
XspI CTAG 1 cut(s) 330
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.