Rroxscaffold_2G00125470

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002004
Physical Location & Seq
Forward (+)
60240945 .. 60247482
6538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00125470.1

Sequence Viewer

Length: 318 bp
ATGGATACGAGGCTAACCGAGGGGGTAGTATTAAAGATCACGGCGGAGGACCAGTTCGAAGGGCTCTCACCGAGGAGGAGGATGTTGGCCCCGCTCGCTTGGAAAGAGTATGATGAAGAAAATCCAAGAAGTACCACTAAATACGACCACCATTTTCACCTTGCTTGCATTCTTGAATGGATGGAAAGAAGTGACTCATGTCCGTGTGCGTCAGAAGTGATATTTGATCCTCCTATTGATTTATGGTATCAAGTCGTGCAGTTGGTTCAGTACTTGAAATTCACCGTGGTTGCTGAGAATGATTGGTGTTTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

105

Amino Acids

12.37

Weight (kDa)

4.7

Isoelectric Point (pI)

59.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 41 - 69 2.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 94
AciI CCGC 2 cut(s) 44, 92
AclWI GGATC 1 cut(s) 221
AcsI RAATTY 1 cut(s) 278
AfaI GTAC 2 cut(s) 133, 272
AgsI TTSAA 2 cut(s) 176, 277
AloI GAACNNNNNNTCC 2 cut(s) 38, 70
AlwI GGATC 1 cut(s) 221
AoxI GGCC 1 cut(s) 87
ApoI RAATTY 1 cut(s) 278
AspS9I GGNCC 2 cut(s) 49, 88
AsuHPI GGTGA 3 cut(s) 60, 149, 274
AsuII TTCGAA 1 cut(s) 57
AvaII GGWCC 1 cut(s) 49
BanII GRGCYC 1 cut(s) 66
BccI CCATC 1 cut(s) 175
BceAI ACGGC 1 cut(s) 57
BmcAI AGTACT 1 cut(s) 272
Bme18I GGWCC 1 cut(s) 49
BmgT120I GGNCC 2 cut(s) 49, 88
BmiI GGNNCC 1 cut(s) 90
BoxI GACNNNNGTC 1 cut(s) 198
Bpu14I TTCGAA 1 cut(s) 57
BsaJI CCNNGG 3 cut(s) 18, 71, 285
BsaXI ACNNNNNCTCC 2 cut(s) 38, 68
Bse1I ACTGG 1 cut(s) 52
BseDI CCNNGG 3 cut(s) 18, 71, 285
BseGI GGATG 2 cut(s) 87, 186
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 52
BseRI GAGGAG 2 cut(s) 88, 91
BsgI GTGCAG 1 cut(s) 278
BshFI GGCC 1 cut(s) 89
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 1 cut(s) 89
Bsp119I TTCGAA 1 cut(s) 57
Bsp1286I GDGCHC 1 cut(s) 66
Bsp143I GATC 2 cut(s) 36, 226
BspACI CCGC 2 cut(s) 44, 92
BspANI GGCC 1 cut(s) 89
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 1 cut(s) 221
BspT104I TTCGAA 1 cut(s) 57
BsrBI CCGCTC 1 cut(s) 94
BsrI ACTGG 1 cut(s) 52
BssECI CCNNGG 3 cut(s) 18, 71, 285
BssMI GATC 2 cut(s) 36, 226
Bst4CI ACNGT 1 cut(s) 286
BstBI TTCGAA 1 cut(s) 57
BstC8I GCNNGC 2 cut(s) 96, 166
BstDEI CTNAG 1 cut(s) 294
BstDSI CCRYGG 1 cut(s) 285
BstF5I GGATG 2 cut(s) 87, 186
BstKTI GATC 2 cut(s) 39, 229
BstMBI GATC 2 cut(s) 36, 226
BstMWI GCNNNNNNNGC 1 cut(s) 95
BstPAI GACNNNNGTC 1 cut(s) 198
BsuRI GGCC 1 cut(s) 89
BtgI CCRYGG 1 cut(s) 285
BtsCI GGATG 2 cut(s) 87, 186
Cac8I GCNNGC 2 cut(s) 96, 166
Cfr13I GGNCC 2 cut(s) 49, 88
CseI GACGC 1 cut(s) 198
Csp6I GTAC 2 cut(s) 132, 271
CviAII CATG 1 cut(s) 198
CviJI RGCY 3 cut(s) 13, 64, 89
CviKI_1 RGCY 3 cut(s) 13, 64, 89
CviQI GTAC 2 cut(s) 132, 271
DdeI CTNAG 1 cut(s) 294
DpnI GATC 2 cut(s) 38, 228
DpnII GATC 2 cut(s) 36, 226
EciI GGCGGA 1 cut(s) 59
Eco24I GRGCYC 1 cut(s) 66
Eco47I GGWCC 1 cut(s) 49
EcoT38I GRGCYC 1 cut(s) 66
FaeI CATG 1 cut(s) 201
FaiI YATR 3 cut(s) 111, 199, 244
FatI CATG 1 cut(s) 197
FauI CCCGC 1 cut(s) 99
FokI GGATG 2 cut(s) 94, 193
FriOI GRGCYC 1 cut(s) 66
HaeIII GGCC 1 cut(s) 89
HgaI GACGC 1 cut(s) 198
Hin1II CATG 1 cut(s) 201
HinfI GANTC 1 cut(s) 194
HphI GGTGA 3 cut(s) 60, 149, 274
Hpy188I TCNGA 1 cut(s) 214
Hpy188III TCNNGA 1 cut(s) 173
HpyAV CCTTC 1 cut(s) 53
HpyCH4III ACNGT 1 cut(s) 286
HpyCH4V TGCA 2 cut(s) 168, 259
HpyF10VI GCNNNNNNNGC 1 cut(s) 95
HpyF3I CTNAG 1 cut(s) 294
Hsp92II CATG 1 cut(s) 201
Kzo9I GATC 2 cut(s) 36, 226
LpnPI CCDG 1 cut(s) 65
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 38, 228
MbiI CCGCTC 1 cut(s) 94
MboI GATC 2 cut(s) 36, 226
MboII GAAGA 1 cut(s) 128
MhlI GDGCHC 1 cut(s) 66
MluCI AATT 1 cut(s) 278
MlyI GAGTC 1 cut(s) 188
MnlI CCTC 7 cut(s) 3, 13, 40, 66, 69, 72, 240
MseI TTAA 2 cut(s) 32, 316
MslI CAYNNNNRTG 1 cut(s) 202
Mva1269I GAATGC 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 95
NdeII GATC 2 cut(s) 36, 226
NlaIII CATG 1 cut(s) 201
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 57
PctI GAATGC 1 cut(s) 168
PleI GAGTC 1 cut(s) 188
PpsI GAGTC 1 cut(s) 188
PshAI GACNNNNGTC 1 cut(s) 198
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 2 cut(s) 49, 88
RsaI GTAC 2 cut(s) 133, 272
RsaNI GTAC 2 cut(s) 132, 271
RseI CAYNNNNRTG 1 cut(s) 202
SaqAI TTAA 2 cut(s) 32, 316
Sau3AI GATC 2 cut(s) 36, 226
Sau96I GGNCC 2 cut(s) 49, 88
ScaI AGTACT 1 cut(s) 272
SchI GAGTC 1 cut(s) 188
SduI GDGCHC 1 cut(s) 66
SetI ASST 1 cut(s) 162
SfuI TTCGAA 1 cut(s) 57
SinI GGWCC 1 cut(s) 49
SmiMI CAYNNNNRTG 1 cut(s) 202
Sse9I AATT 1 cut(s) 278
SsiI CCGC 2 cut(s) 44, 92
TaaI ACNGT 1 cut(s) 286
TaqI TCGA 1 cut(s) 57
TasI AATT 1 cut(s) 278
TatI WGTACW 1 cut(s) 270
Tru1I TTAA 2 cut(s) 32, 316
Tru9I TTAA 2 cut(s) 32, 316
TseFI GTSAC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 1 cut(s) 129
TspGWI ACGGA 1 cut(s) 192
VpaK11BI GGWCC 1 cut(s) 49
XapI RAATTY 1 cut(s) 278
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.