Rh4BG291300

Anaphase-promoting complex subunit 11 RING-H2 finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0002004
Physical Location & Seq
Forward (+)
46367342 .. 46370292
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG291300.1

Sequence Viewer

Length: 585 bp
ATGTTCTTGCAGTATAGTGCACCGTTTCAAAGGCTTGATGGGAGATCAGTCCCATCACCTAACCAAGGGGCTACTTCTTCTGTCCCATCTGGAACTGGCACAACAGTAGCTAATAACTCCTCAAATGAGACTCAATTCTCAGTGGCCAGGCCATCACCCTTTGATGCCGATCAGAGGTACTCACGTTTGCAGCGCGACGGCTTGATCTCTAGGCGTGAGAAGTCAATGACACATTTGCAGGAAGATGCACAACAAGTGAGAAGAAGCAGTTCTGGGACAGAGTCCCTGGGTTCGGGGAAGAAACAGAATGGAGTTGATACTGAAGAAGATTGCAAGTTAGGTCAATCAGAGACCTCAGAAAAGGATCTAGCATCAAAAGTCGCATATGGACTAACTTATGTGCAGCCATCTTCTGAAGATGATGATGTCTGCCCTACATGTCTTGATGATTATACCCCTGAAAATCCTAAAATCACAACAAGATGTTCTCACCATTTCCATCTTGGCTGTATTTATGAGTGGTTGGAAAGAAGTGAAAGTTGTCCAATGTGTGGAAAGGAGATGGAGTTCTGTGAAAGCCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_2 zf-rbx1

Protein Analysis

194

Amino Acids

21.48

Weight (kDa)

4.95

Isoelectric Point (pI)

65.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 141 - 184 3.9e-11 RING-H2 zinc finger domain
zf-ANAPC11 PF12861 141 - 190 2e-07 Anaphase-promoting complex subunit 11 RING-H2 finger
zf-RING_2 PF13639 143 - 184 1.2e-11 Ring finger domain
zf-C3HC4_2 PF13923 144 - 184 6.8e-08 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 144 - 184 8.6e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_UBOX PF13445 144 - 182 6.8e-06 RING-type zinc-finger
zf-RING_11 PF17123 144 - 171 6.4e-06 RING-like zinc finger
zf-RING_5 PF14634 144 - 186 8.8e-06 zinc-RING finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 551
AccII CGCG 1 cut(s) 195
AclWI GGATC 1 cut(s) 372
AcoI YGGCCR 1 cut(s) 144
AcuI CTGAAG 2 cut(s) 342, 435
AfaI GTAC 1 cut(s) 179
AfiI CCNNNNNNNGG 4 cut(s) 65, 174, 292, 551
AflIII ACRYGT 1 cut(s) 437
AgsI TTSAA 1 cut(s) 29
AjnI CCWGG 2 cut(s) 146, 285
AloI GAACNNNNNNTCC 2 cut(s) 551, 583
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw21I GWGCWC 1 cut(s) 22
Alw26I GTCTC 2 cut(s) 122, 344
Alw44I GTGCAC 1 cut(s) 18
AlwI GGATC 1 cut(s) 372
AoxI GGCC 2 cut(s) 144, 149
ApaLI GTGCAC 1 cut(s) 18
ApeKI GCWGC 2 cut(s) 190, 403
Asp700I GAANNNNTTC 1 cut(s) 268
AspLEI GCGC 1 cut(s) 195
AsuHPI GGTGA 3 cut(s) 48, 147, 482
BaeGI GKGCMC 1 cut(s) 22
BaeI ACNNNNGTAYC 2 cut(s) 169, 202
BalI TGGCCA 1 cut(s) 146
Bbv12I GWGCWC 1 cut(s) 22
BbvI GCAGC 2 cut(s) 202, 415
BccI CCATC 7 cut(s) 32, 61, 94, 160, 415, 507, 556
BceAI ACGGC 1 cut(s) 214
BciT130I CCWGG 2 cut(s) 148, 287
BcoDI GTCTC 2 cut(s) 122, 344
BfaI CTAG 2 cut(s) 210, 368
BisI GCNGC 2 cut(s) 191, 404
BlsI GCNGC 2 cut(s) 192, 405
Bme1390I CCNGG 2 cut(s) 148, 287
BmrFI CCNGG 2 cut(s) 148, 287
BmsI GCATC 3 cut(s) 154, 235, 380
BsaBI GATNNNNATC 1 cut(s) 168
BsaI GGTCTC 1 cut(s) 344
BsaJI CCNNGG 3 cut(s) 64, 285, 286
BsaXI ACNNNNNCTCC 2 cut(s) 551, 581
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 174, 292, 551
Bse1I ACTGG 1 cut(s) 100
Bse8I GATNNNNATC 1 cut(s) 168
BseBI CCWGG 2 cut(s) 148, 287
BseDI CCNNGG 3 cut(s) 64, 285, 286
BseJI GATNNNNATC 1 cut(s) 168
BseLI CCNNNNNNNGG 4 cut(s) 65, 174, 292, 551
BseMII CTCAG 2 cut(s) 153, 369
BseNI ACTGG 1 cut(s) 100
BseRI GAGGAG 1 cut(s) 109
BseSI GKGCMC 1 cut(s) 22
BseXI GCAGC 2 cut(s) 202, 415
BsgI GTGCAG 1 cut(s) 422
Bsh1236I CGCG 1 cut(s) 195
BshFI GGCC 2 cut(s) 146, 151
BsiHKAI GWGCWC 1 cut(s) 22
BslFI GGGAC 4 cut(s) 35, 68, 268, 289
BslI CCNNNNNNNGG 4 cut(s) 65, 174, 292, 551
BsmAI GTCTC 2 cut(s) 122, 344
BsmFI GGGAC 4 cut(s) 35, 68, 268, 289
BsnI GGCC 2 cut(s) 146, 151
Bso31I GGTCTC 1 cut(s) 344
Bsp1286I GDGCHC 1 cut(s) 22
Bsp143I GATC 4 cut(s) 44, 169, 204, 364
BspANI GGCC 2 cut(s) 146, 151
BspCNI CTCAG 2 cut(s) 152, 368
BspFNI CGCG 1 cut(s) 195
BspPI GGATC 1 cut(s) 372
BspTNI GGTCTC 1 cut(s) 344
BsrI ACTGG 1 cut(s) 100
BssECI CCNNGG 3 cut(s) 64, 285, 286
BssMI GATC 4 cut(s) 44, 169, 204, 364
BssT1I CCWWGG 1 cut(s) 64
Bst2UI CCWGG 2 cut(s) 148, 287
Bst4CI ACNGT 2 cut(s) 24, 106
BstDEI CTNAG 2 cut(s) 139, 355
BstENI CCTNNNNNAGG 1 cut(s) 63
BstFNI CGCG 1 cut(s) 195
BstHHI GCGC 1 cut(s) 195
BstKTI GATC 4 cut(s) 47, 172, 207, 367
BstMAI GTCTC 2 cut(s) 122, 344
BstMBI GATC 4 cut(s) 44, 169, 204, 364
BstNI CCWGG 2 cut(s) 148, 287
BstNSI RCATGY 1 cut(s) 441
BstSCI CCNGG 2 cut(s) 146, 285
BstSLI GKGCMC 1 cut(s) 22
BstUI CGCG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 202, 415
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuRI GGCC 2 cut(s) 146, 151
BtsIMutI CAGTG 1 cut(s) 147
CfoI GCGC 1 cut(s) 195
Csp6I GTAC 1 cut(s) 178
CviAII CATG 1 cut(s) 438
CviJI RGCY 9 cut(s) 34, 71, 110, 146, 151, 201, 406, 507, 579
CviKI_1 RGCY 9 cut(s) 34, 71, 110, 146, 151, 201, 406, 507, 579
CviQI GTAC 1 cut(s) 178
DdeI CTNAG 2 cut(s) 139, 355
DpnI GATC 4 cut(s) 46, 171, 206, 366
DpnII GATC 4 cut(s) 44, 169, 204, 364
EaeI YGGCCR 1 cut(s) 144
Eco130I CCWWGG 1 cut(s) 64
Eco31I GGTCTC 1 cut(s) 344
Eco57I CTGAAG 2 cut(s) 342, 435
EcoNI CCTNNNNNAGG 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 146, 285
EcoT14I CCWWGG 1 cut(s) 64
ErhI CCWWGG 1 cut(s) 64
FaeI CATG 1 cut(s) 441
FaiI YATR 7 cut(s) 15, 385, 387, 399, 439, 453, 516
FaqI GGGAC 4 cut(s) 35, 68, 268, 289
FatI CATG 1 cut(s) 437
FauNDI CATATG 1 cut(s) 385
Fnu4HI GCNGC 2 cut(s) 191, 404
Fsp4HI GCNGC 2 cut(s) 191, 404
FspBI CTAG 2 cut(s) 210, 368
GlaI GCGC 1 cut(s) 194
GluI GCNGC 2 cut(s) 191, 404
HaeIII GGCC 2 cut(s) 146, 151
HhaI GCGC 1 cut(s) 195
Hin1II CATG 1 cut(s) 441
Hin6I GCGC 1 cut(s) 193
HinP1I GCGC 1 cut(s) 193
HinfI GANTC 2 cut(s) 130, 281
HphI GGTGA 3 cut(s) 48, 147, 482
Hpy166II GTNNAC 1 cut(s) 20
Hpy188I TCNGA 4 cut(s) 174, 349, 358, 415
Hpy188III TCNNGA 2 cut(s) 90, 443
Hpy8I GTNNAC 1 cut(s) 20
Hpy99I CGWCG 1 cut(s) 200
HpyCH4III ACNGT 2 cut(s) 24, 106
HpyCH4IV ACGT 1 cut(s) 184
HpyCH4V TGCA 7 cut(s) 10, 20, 190, 238, 248, 333, 403
HpyF3I CTNAG 2 cut(s) 139, 355
HpySE526I ACGT 1 cut(s) 184
Hsp92II CATG 1 cut(s) 441
HspAI GCGC 1 cut(s) 193
Kzo9I GATC 4 cut(s) 44, 169, 204, 364
LpnPI CCDG 9 cut(s) 75, 81, 133, 160, 224, 258, 272, 299, 471
Lsp1109I GCAGC 2 cut(s) 202, 415
LweI GCATC 3 cut(s) 154, 235, 380
MaeI CTAG 2 cut(s) 210, 368
MaeII ACGT 1 cut(s) 184
MalI GATC 4 cut(s) 46, 171, 206, 366
MboI GATC 4 cut(s) 44, 169, 204, 364
MboII GAAGA 8 cut(s) 69, 254, 273, 310, 335, 338, 402, 428
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 1 cut(s) 22
MlsI TGGCCA 1 cut(s) 146
MluCI AATT 1 cut(s) 134
MluNI TGGCCA 1 cut(s) 146
MlyI GAGTC 2 cut(s) 124, 290
MmeI TCCRAC 1 cut(s) 504
MnlI CCTC 3 cut(s) 130, 168, 364
Mox20I TGGCCA 1 cut(s) 146
MroXI GAANNNNTTC 1 cut(s) 268
MscI TGGCCA 1 cut(s) 146
Msp20I TGGCCA 1 cut(s) 146
MspR9I CCNGG 2 cut(s) 148, 287
MvaI CCWGG 2 cut(s) 148, 287
MvnI CGCG 1 cut(s) 195
NdeI CATATG 1 cut(s) 385
NdeII GATC 4 cut(s) 44, 169, 204, 364
NlaIII CATG 1 cut(s) 441
NspI RCATGY 1 cut(s) 441
PasI CCCWGGG 1 cut(s) 286
PciI ACATGT 1 cut(s) 437
PdmI GAANNNNTTC 1 cut(s) 268
PflFI GACNNNGTC 1 cut(s) 280
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 2 cut(s) 192, 405
PleI GAGTC 2 cut(s) 124, 289
PpsI GAGTC 2 cut(s) 124, 289
PscI ACATGT 1 cut(s) 437
Psp6I CCWGG 2 cut(s) 146, 285
PspGI CCWGG 2 cut(s) 146, 285
PsuI RGATCY 1 cut(s) 364
PsyI GACNNNGTC 1 cut(s) 280
RsaI GTAC 1 cut(s) 179
RsaNI GTAC 1 cut(s) 178
SatI GCNGC 2 cut(s) 191, 404
Sau3AI GATC 4 cut(s) 44, 169, 204, 364
SchI GAGTC 2 cut(s) 124, 290
ScrFI CCNGG 2 cut(s) 148, 287
SduI GDGCHC 1 cut(s) 22
SetI ASST 6 cut(s) 61, 112, 179, 187, 343, 356
SfaNI GCATC 3 cut(s) 154, 235, 380
Sse9I AATT 1 cut(s) 134
SspMI CTAG 2 cut(s) 210, 368
StyD4I CCNGG 2 cut(s) 146, 285
StyI CCWWGG 1 cut(s) 64
TaaI ACNGT 2 cut(s) 24, 106
TaiI ACGT 1 cut(s) 187
TasI AATT 1 cut(s) 134
TscAI CASTG 1 cut(s) 147
TseI GCWGC 2 cut(s) 190, 403
TspRI CASTG 1 cut(s) 147
Tth111I GACNNNGTC 1 cut(s) 280
Van91I CCANNNNNTGG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 18
XagI CCTNNNNNAGG 1 cut(s) 63
XceI RCATGY 1 cut(s) 441
XcmI CCANNNNNNNNNTGG 1 cut(s) 500
XmnI GAANNNNTTC 1 cut(s) 268
XspI CTAG 2 cut(s) 210, 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.