AT1G75280

Isoflavone reductase homolog P3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0003310
Physical Location & Seq
Forward (+)
28251626 .. 28253686
2061 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G75280.1

Sequence Viewer

Length: 933 bp
ATGGCGACGGAGAAAAGCAAGATTCTGGTGATCGGAGGAACTGGTTACATCGGGAAATTCCTAGTAGAAGCGAGCGCCAAAGCTGGCCACTCCACATTCGCTCTCGTTAGAGAAGCAACTCTATCCGATCCTGTCAAGGGCAAAACCGTCCAGAGTTTCAAAGATCTCGGCGTCACAATACTACACGGAGATTTGAATGATCACGAGAGCTTAGTGAAGGCTATTAAACAGGTTGATGTGGTGATATCTACCGTTGGGAGCATGCAAATCTTGGATCAAACCAAGATCATTTCCGCCATCAAAGAAGCCGGTAATGTCAAGAGATTCTTGCCGTCTGAGTTTGGGGTGGATGTGGATAGGACGAGTGCGGTTGAGCCAGCTAAATCGGCTTTTGCAGGGAAGATACAGATCAGGAGAACCATCGAAGCGGAAGGAATACCATACACTTACGCTGTTACCGGTTGCTTTGGTGGTTACTACTTGCCAACATTGGTTCAGTTCGAGCCTGGTCTCACTTCTCCTCCTAGAGACAAAGTCACCATTCTTGGCGATGGAAATGCCAAAGCTGTGATCAACAAAGAGGAAGATATTGCTGCTTACACGATCAAGGCGGTGGATGATCCTAGGACTCTGAACAAAATCCTATACATTAAGCCTTCTAACAACACTTTATCGATGAACGAAATAGTCACCTTGTGGGAGAAAAAGATTGGCAAGTCTCTTGAGAAGACTCACCTCCCAGAGGAACAACTACTTAAAAGCATCCAAGAGTCTCCGATTCCCATCAATGTTGTTCTGTCGATAAACCACGCGGTGTTTGTGAATGGAGATACCAATATCAGTATAGAGCCTTCCTTTGGTGTGGAAGCCTCTGAGCTTTACCCTGATGTCAAGTACACAAGTGTTGACGAGTATCTCAGTTATTTCGCTTGA

Protein Analysis

310

Amino Acids

33.74

Weight (kDa)

5.66

Isoelectric Point (pI)

29.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 306 6.5e-104 NmrA-like family
Epimerase PF01370 8 - 86 4.8e-09 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 9 - 79 2.2e-06 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 9 - 92 3.8e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 150 9.5e-15 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 812
AciI CCGC 5 cut(s) 294, 368, 428, 611, 812
AclWI GGATC 3 cut(s) 122, 282, 614
AcoI YGGCCR 1 cut(s) 85
AcsI RAATTY 1 cut(s) 56
AcyI GRCGYC 1 cut(s) 171
AdeI CACNNNGTG 2 cut(s) 696, 814
AfaI GTAC 1 cut(s) 896
AfiI CCNNNNNNNGG 3 cut(s) 137, 742, 857
AgeI ACCGGT 1 cut(s) 458
AgsI TTSAA 2 cut(s) 160, 196
AjnI CCWGG 1 cut(s) 505
AluBI AGCT 5 cut(s) 83, 210, 380, 566, 877
AluI AGCT 5 cut(s) 83, 210, 380, 566, 877
Alw26I GTCTC 4 cut(s) 515, 522, 723, 777
AlwI GGATC 3 cut(s) 122, 282, 614
AoxI GGCC 1 cut(s) 85
ApeKI GCWGC 1 cut(s) 593
ApoI RAATTY 1 cut(s) 56
AsiGI ACCGGT 1 cut(s) 458
AspA2I CCTAGG 1 cut(s) 623
AspLEI GCGC 1 cut(s) 77
AsuHPI GGTGA 5 cut(s) 40, 253, 529, 682, 725
AvrII CCTAGG 1 cut(s) 623
BalI TGGCCA 1 cut(s) 87
BarI GAAGNNNNNNTAC 2 cut(s) 835, 867
BauI CACGAG 1 cut(s) 203
BbsI GAAGAC 1 cut(s) 734
BbvI GCAGC 1 cut(s) 580
BccI CCATC 4 cut(s) 305, 428, 545, 791
BceAI ACGGC 1 cut(s) 316
BcgI CGANNNNNNTGC 2 cut(s) 539, 573
BciT130I CCWGG 1 cut(s) 507
BclI TGATCA 2 cut(s) 199, 570
BcoDI GTCTC 4 cut(s) 515, 522, 723, 777
BfaI CTAG 3 cut(s) 62, 525, 624
BfoI RGCGCY 1 cut(s) 78
BglII AGATCT 1 cut(s) 163
BisI GCNGC 1 cut(s) 594
BlnI CCTAGG 1 cut(s) 623
BlsI GCNGC 1 cut(s) 595
Bme1390I CCNGG 1 cut(s) 507
BmrFI CCNGG 1 cut(s) 507
BmsI GCATC 1 cut(s) 771
BpiI GAAGAC 1 cut(s) 734
BpuEI CTTGAG 1 cut(s) 743
Bsa29I ATCGAT 1 cut(s) 674
BsaBI GATNNNNATC 2 cut(s) 407, 782
BsaHI GRCGYC 1 cut(s) 171
BsaI GGTCTC 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 623
BsaWI WCCGGW 1 cut(s) 458
BsaXI ACNNNNNCTCC 2 cut(s) 505, 535
Bsc4I CCNNNNNNNGG 3 cut(s) 137, 742, 857
Bse118I RCCGGY 2 cut(s) 308, 458
Bse1I ACTGG 1 cut(s) 46
Bse8I GATNNNNATC 2 cut(s) 407, 782
BseBI CCWGG 1 cut(s) 507
BseCI ATCGAT 1 cut(s) 674
BseDI CCNNGG 1 cut(s) 623
BseGI GGATG 3 cut(s) 355, 622, 762
BseJI GATNNNNATC 2 cut(s) 407, 782
BseLI CCNNNNNNNGG 3 cut(s) 137, 742, 857
BseMII CTCAG 3 cut(s) 327, 864, 931
BseNI ACTGG 1 cut(s) 46
BseRI GAGGAG 1 cut(s) 510
BseXI GCAGC 1 cut(s) 580
Bsh1236I CGCG 1 cut(s) 812
BshFI GGCC 1 cut(s) 87
BshTI ACCGGT 1 cut(s) 458
BshVI ATCGAT 1 cut(s) 674
BsiSI CCGG 2 cut(s) 309, 459
BslI CCNNNNNNNGG 3 cut(s) 137, 742, 857
BsmAI GTCTC 4 cut(s) 515, 522, 723, 777
BsnI GGCC 1 cut(s) 87
Bso31I GGTCTC 1 cut(s) 515
BspACI CCGC 5 cut(s) 294, 368, 428, 611, 812
BspANI GGCC 1 cut(s) 87
BspCNI CTCAG 3 cut(s) 328, 865, 930
BspDI ATCGAT 1 cut(s) 674
BspFNI CGCG 1 cut(s) 812
BspPI GGATC 3 cut(s) 122, 282, 614
BspTNI GGTCTC 1 cut(s) 515
BsrFI RCCGGY 2 cut(s) 308, 458
BsrI ACTGG 1 cut(s) 46
BssAI RCCGGY 2 cut(s) 308, 458
BssECI CCNNGG 1 cut(s) 623
BssNI GRCGYC 1 cut(s) 171
BssSI CACGAG 1 cut(s) 203
BssT1I CCWWGG 1 cut(s) 623
Bst2BI CACGAG 1 cut(s) 203
Bst2UI CCWGG 1 cut(s) 507
Bst4CI ACNGT 2 cut(s) 148, 253
BstACI GRCGYC 1 cut(s) 171
BstC8I GCNNGC 4 cut(s) 73, 85, 263, 378
BstDEI CTNAG 4 cut(s) 211, 336, 873, 917
BstENI CCTNNNNNAGG 1 cut(s) 740
BstF5I GGATG 3 cut(s) 355, 622, 762
BstFNI CGCG 1 cut(s) 812
BstH2I RGCGCY 1 cut(s) 78
BstHHI GCGC 1 cut(s) 77
BstMAI GTCTC 4 cut(s) 515, 522, 723, 777
BstMWI GCNNNNNNNGC 1 cut(s) 386
BstNI CCWGG 1 cut(s) 507
BstNSI RCATGY 1 cut(s) 265
BstSCI CCNGG 1 cut(s) 505
BstUI CGCG 1 cut(s) 812
BstV1I GCAGC 1 cut(s) 580
BstV2I GAAGAC 1 cut(s) 734
BstX2I RGATCY 1 cut(s) 163
BstYI RGATCY 1 cut(s) 163
Bsu15I ATCGAT 1 cut(s) 674
BsuRI GGCC 1 cut(s) 87
BsuTUI ATCGAT 1 cut(s) 674
BtgZI GCGATG 1 cut(s) 564
BtsCI GGATG 3 cut(s) 355, 622, 762
Cac8I GCNNGC 4 cut(s) 73, 85, 263, 378
CfoI GCGC 1 cut(s) 77
Cfr10I RCCGGY 2 cut(s) 308, 458
ClaI ATCGAT 1 cut(s) 674
CseI GACGC 1 cut(s) 160
Csp6I GTAC 1 cut(s) 895
CspAI ACCGGT 1 cut(s) 458
CviAII CATG 1 cut(s) 262
CviQI GTAC 1 cut(s) 895
DdeI CTNAG 4 cut(s) 211, 336, 873, 917
DraIII CACNNNGTG 2 cut(s) 696, 814
EaeI YGGCCR 1 cut(s) 85
EciI GGCGGA 1 cut(s) 283
Eco130I CCWWGG 1 cut(s) 623
Eco31I GGTCTC 1 cut(s) 515
Eco32I GATATC 1 cut(s) 246
EcoNI CCTNNNNNAGG 1 cut(s) 740
EcoRII CCWGG 1 cut(s) 505
EcoRV GATATC 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 623
FaeI CATG 1 cut(s) 265
FaiI YATR 4 cut(s) 263, 442, 646, 845
FalI AAGNNNNNCTT 2 cut(s) 311, 343
FatI CATG 1 cut(s) 261
FbaI TGATCA 2 cut(s) 199, 570
Fnu4HI GCNGC 1 cut(s) 594
FokI GGATG 3 cut(s) 362, 629, 749
Fsp4HI GCNGC 1 cut(s) 594
FspBI CTAG 3 cut(s) 62, 525, 624
GlaI GCGC 1 cut(s) 76
GluI GCNGC 1 cut(s) 594
HaeII RGCGCY 1 cut(s) 78
HaeIII GGCC 1 cut(s) 87
HapII CCGG 2 cut(s) 309, 459
HgaI GACGC 1 cut(s) 160
HhaI GCGC 1 cut(s) 77
Hin1I GRCGYC 1 cut(s) 171
Hin1II CATG 1 cut(s) 265
Hin6I GCGC 1 cut(s) 75
HinP1I GCGC 1 cut(s) 75
HincII GTYRAC 1 cut(s) 907
HindII GTYRAC 1 cut(s) 907
HinfI GANTC 6 cut(s) 22, 324, 628, 730, 770, 778
HpaII CCGG 2 cut(s) 309, 459
HphI GGTGA 5 cut(s) 40, 253, 529, 682, 725
Hpy166II GTNNAC 2 cut(s) 897, 907
Hpy188I TCNGA 6 cut(s) 35, 127, 337, 633, 777, 874
Hpy188III TCNNGA 6 cut(s) 52, 151, 203, 319, 412, 722
Hpy8I GTNNAC 2 cut(s) 897, 907
Hpy99I CGWCG 1 cut(s) 10
HpyAV CCTTC 4 cut(s) 211, 425, 666, 861
HpyCH4III ACNGT 2 cut(s) 148, 253
HpyCH4V TGCA 2 cut(s) 265, 395
HpyF10VI GCNNNNNNNGC 1 cut(s) 386
HpyF3I CTNAG 4 cut(s) 211, 336, 873, 917
Hsp92I GRCGYC 1 cut(s) 171
Hsp92II CATG 1 cut(s) 265
HspAI GCGC 1 cut(s) 75
Ksp22I TGATCA 2 cut(s) 199, 570
LmnI GCTCC 1 cut(s) 258
Lsp1109I GCAGC 1 cut(s) 580
LweI GCATC 1 cut(s) 771
MaeI CTAG 3 cut(s) 62, 525, 624
MaeIII GTNAC 6 cut(s) 44, 172, 454, 473, 535, 688
MboII GAAGA 3 cut(s) 412, 596, 739
MflI RGATCY 1 cut(s) 163
MlsI TGGCCA 1 cut(s) 87
MluCI AATT 1 cut(s) 56
MluNI TGGCCA 1 cut(s) 87
MlyI GAGTC 3 cut(s) 622, 724, 779
MnlI CCTC 6 cut(s) 29, 531, 574, 736, 746, 880
Mox20I TGGCCA 1 cut(s) 87
MscI TGGCCA 1 cut(s) 87
MseI TTAA 3 cut(s) 225, 651, 756
Msp20I TGGCCA 1 cut(s) 87
MspI CCGG 2 cut(s) 309, 459
MspR9I CCNGG 1 cut(s) 507
MvaI CCWGG 1 cut(s) 507
MvnI CGCG 1 cut(s) 812
MwoI GCNNNNNNNGC 1 cut(s) 386
NlaIII CATG 1 cut(s) 265
NmeAIII GCCGAG 1 cut(s) 147
NmuCI GTSAC 3 cut(s) 172, 535, 688
NspI RCATGY 1 cut(s) 265
PaeI GCATGC 1 cut(s) 265
PfeI GAWTC 3 cut(s) 22, 324, 778
PflFI GACNNNGTC 1 cut(s) 533
PinAI ACCGGT 1 cut(s) 458
PkrI GCNGC 1 cut(s) 595
PleI GAGTC 3 cut(s) 622, 724, 778
PpsI GAGTC 3 cut(s) 622, 724, 778
Psp6I CCWGG 1 cut(s) 505
PspGI CCWGG 1 cut(s) 505
PsuI RGATCY 1 cut(s) 163
PsyI GACNNNGTC 1 cut(s) 533
RsaI GTAC 1 cut(s) 896
RsaNI GTAC 1 cut(s) 895
SaqAI TTAA 3 cut(s) 225, 651, 756
SatI GCNGC 1 cut(s) 594
SchI GAGTC 3 cut(s) 622, 724, 779
ScrFI CCNGG 1 cut(s) 507
SetI ASST 8 cut(s) 85, 212, 234, 382, 568, 695, 738, 879
SfaNI GCATC 1 cut(s) 771
SmlI CTYRAG 1 cut(s) 722
SmoI CTYRAG 1 cut(s) 722
SphI GCATGC 1 cut(s) 265
Sse9I AATT 1 cut(s) 56
SsiI CCGC 5 cut(s) 294, 368, 428, 611, 812
SspMI CTAG 3 cut(s) 62, 525, 624
StyD4I CCNGG 1 cut(s) 505
StyI CCWWGG 1 cut(s) 623
TaaI ACNGT 2 cut(s) 148, 253
TaqI TCGA 4 cut(s) 423, 501, 674, 800
TasI AATT 1 cut(s) 56
TatI WGTACW 1 cut(s) 894
TfiI GAWTC 3 cut(s) 22, 324, 778
Tru1I TTAA 3 cut(s) 225, 651, 756
Tru9I TTAA 3 cut(s) 225, 651, 756
TseFI GTSAC 3 cut(s) 172, 535, 688
TseI GCWGC 1 cut(s) 593
Tsp45I GTSAC 3 cut(s) 172, 535, 688
TspDTI ATGAA 1 cut(s) 692
TspGWI ACGGA 2 cut(s) 23, 201
Tth111I GACNNNGTC 1 cut(s) 533
XagI CCTNNNNNAGG 1 cut(s) 740
XapI RAATTY 1 cut(s) 56
XceI RCATGY 1 cut(s) 265
XmaJI CCTAGG 1 cut(s) 623
XspI CTAG 3 cut(s) 62, 525, 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.