Rorug06G0303000

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003310
Physical Location & Seq
Reverse (-)
45428141 .. 45430548
2408 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0303000.1

Sequence Viewer

Length: 1191 bp
ATGTCACAGGGCGTTCATCTCATCTGTTCTGGACCATCAGATAGCAAATGTCTACCTTGTTCTGGGATTTCGGGGAGAGTAGCCCCTGTATATCCTTGCTCTTCTCGTTTCAACCATAGATTCAAGTCGCAGGTCAGGGTTGGATCACTAAGAGGCGCTTATACTGGGGTGTCTTTTACGTGCAGAGCAGGTTCTAGTGGTCGTAATAGAAATCCAGACTTCCCAAGGCAAAACAGGCAAGGGTACTCTCGGGGGAGGAACAGGAGAAATGAAGAGAGAGACAGCTTTGAAAACCTTGAAGAATCTGATTTGCTTTCTGCTAAAAATGGTCCACTGCTCTCCCTCCCTAGTAACACAAAGTTTGGGGCTACTGCAGCCCCTGGTCCCAGAGAAAAAGAGATTGTTGAGCTGTTCAGGAAAGTCCAGGCTCAGCTTCGGGAGAGAACTGCTGTTAAAGAAGAAAAGAAGGTTGAACCCGTGCAAGGGCAAGGTAAAGATAATGGAACTGTGGATTCTCTTCTTAAACTACTAAGGAAACATTCGGCTGAACAGGCAAAGAGAAACAGTAATGGGGTTGGAAACAAAGACTTTATGTTGGACCAACCAGAAAAGACTGGCCGATATAGTGAAAGAAAAAACACGGCTTCTTTGGATTCAATTAACAGTGTGAAGGATTATGCTGAAGAGCCTACTGCCTCTTTGAGCAGGCCTGCATCAAGTTTTCGACGCAAGTCTCCTATCAATCGATTGACATATCAACCTATTTATTCAGAGGATGATGAGATAGTCAATTCGGTGCAGCATGTGAATTCAACTGACAAAAGAAAAAAGAATTATGCTGAGAGAGTTTCTGAACCAGAACCTGAGCCAGAGCCAGAGCTTGAGATTGATGTGGAGTCGGTACTTGAGCCTGAGCCTGTTCATGATACTGAGTTTGGATTTGAACCTGAACCCGAGCCTGAGCTAGAGATTGTTCAGTTGTTAGAGGATGAGACTTCAGGTCCTGAAGAAAATGTTGATGATGAGGAGGACGAAGTCCAAGAGCAACTGATTGAACAGACGGATTTGAGTGCATATAAGCTACCAGAATTGAGGGCACTTGCCAAGTCTCGAGGCGTGAAAGGGTTCTCAAAGATGAAGAAGGCCGAGCTTCTGGAGTTGCTAAGTGGTGGGAGCTTAGATGATTCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

396

Amino Acids

44.23

Weight (kDa)

5.31

Isoelectric Point (pI)

52.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rho_N PF07498 355 - 386 6.4e-07 Rho termination factor, N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 121, 179
AccI GTMKAC 1 cut(s) 52
AclWI GGATC 1 cut(s) 151
AcoI YGGCCR 1 cut(s) 616
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 3 cut(s) 702, 981, 1026
AfaI GTAC 2 cut(s) 245, 903
AfiI CCNNNNNNNGG 3 cut(s) 62, 482, 483
AgsI TTSAA 9 cut(s) 112, 124, 290, 299, 473, 657, 813, 944, 1055
AhdI GACNNNNNGTC 1 cut(s) 999
AjnI CCWGG 2 cut(s) 379, 423
AluBI AGCT 8 cut(s) 285, 409, 433, 880, 964, 1081, 1150, 1176
AluI AGCT 8 cut(s) 285, 409, 433, 880, 964, 1081, 1150, 1176
Alw26I GTCTC 4 cut(s) 273, 738, 986, 1113
AlwI GGATC 1 cut(s) 151
AlwNI CAGNNNCTG 3 cut(s) 380, 863, 1004
Ama87I CYCGRG 3 cut(s) 249, 953, 1110
AoxI GGCC 3 cut(s) 616, 707, 1143
ApeKI GCWGC 2 cut(s) 374, 799
ApoI RAATTY 1 cut(s) 808
Asp700I GAANNNNTTC 1 cut(s) 1124
AspLEI GCGC 1 cut(s) 158
AspS9I GGNCC 5 cut(s) 32, 329, 383, 598, 1001
AvaI CYCGRG 3 cut(s) 249, 953, 1110
AvaII GGWCC 5 cut(s) 32, 329, 383, 598, 1001
BaeGI GKGCMC 1 cut(s) 1099
BbvI GCAGC 2 cut(s) 386, 811
BccI CCATC 1 cut(s) 43
BceAI ACGGC 1 cut(s) 657
BciT130I CCWGG 2 cut(s) 381, 425
BcoDI GTCTC 4 cut(s) 273, 738, 986, 1113
BfaI CTAG 3 cut(s) 195, 348, 965
BfmI CTRYAG 1 cut(s) 372
BfoI RGCGCY 1 cut(s) 159
BfuAI ACCTGC 2 cut(s) 121, 179
BisI GCNGC 2 cut(s) 375, 800
BlpI GCTNAGC 1 cut(s) 429
BlsI GCNGC 2 cut(s) 376, 801
Bme1390I CCNGG 2 cut(s) 381, 425
Bme18I GGWCC 5 cut(s) 32, 329, 383, 598, 1001
BmeRI GACNNNNNGTC 1 cut(s) 999
BmeT110I CYCGRG 3 cut(s) 249, 953, 1110
BmgT120I GGNCC 5 cut(s) 32, 329, 383, 598, 1001
BmiI GGNNCC 1 cut(s) 385
BmrFI CCNGG 2 cut(s) 381, 425
BmrI ACTGGG 1 cut(s) 174
BmsI GCATC 1 cut(s) 722
BmuI ACTGGG 1 cut(s) 174
BoxI GACNNNNGTC 1 cut(s) 730
BpmI CTGGAG 1 cut(s) 1175
Bpu10I CCTNAGC 3 cut(s) 864, 912, 960
Bpu1102I GCTNAGC 1 cut(s) 429
BpuEI CTTGAG 2 cut(s) 902, 926
Bsa29I ATCGAT 1 cut(s) 745
BsaAI YACGTR 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 224, 379
Bsc4I CCNNNNNNNGG 3 cut(s) 62, 482, 483
Bse1I ACTGG 2 cut(s) 169, 619
BseBI CCWGG 2 cut(s) 381, 425
BseCI ATCGAT 1 cut(s) 745
BseDI CCNNGG 2 cut(s) 224, 379
BseGI GGATG 2 cut(s) 781, 994
BseLI CCNNNNNNNGG 3 cut(s) 62, 482, 483
BseMII CTCAG 6 cut(s) 443, 831, 855, 903, 921, 951
BseNI ACTGG 2 cut(s) 169, 619
BseRI GAGGAG 1 cut(s) 1040
BseSI GKGCMC 1 cut(s) 1099
BseXI GCAGC 2 cut(s) 386, 811
BsgI GTGCAG 2 cut(s) 202, 818
BshFI GGCC 3 cut(s) 618, 709, 1145
BshVI ATCGAT 1 cut(s) 745
BsiHKCI CYCGRG 3 cut(s) 249, 953, 1110
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 3 cut(s) 62, 482, 483
BsmAI GTCTC 4 cut(s) 273, 738, 986, 1113
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 3 cut(s) 618, 709, 1145
BsoBI CYCGRG 3 cut(s) 249, 953, 1110
Bsp1286I GDGCHC 1 cut(s) 1099
Bsp143I GATC 1 cut(s) 143
Bsp1720I GCTNAGC 1 cut(s) 429
BspANI GGCC 3 cut(s) 618, 709, 1145
BspCNI CTCAG 6 cut(s) 442, 832, 856, 904, 922, 952
BspDI ATCGAT 1 cut(s) 745
BspHI TCATGA 2 cut(s) 922, 1187
BspLI GGNNCC 1 cut(s) 385
BspMAI CTGCAG 1 cut(s) 376
BspMI ACCTGC 2 cut(s) 121, 179
BspPI GGATC 1 cut(s) 151
BspQI GCTCTTC 2 cut(s) 106, 678
BsrI ACTGG 2 cut(s) 169, 619
BssECI CCNNGG 2 cut(s) 224, 379
BssMI GATC 1 cut(s) 143
BssT1I CCWWGG 1 cut(s) 224
Bst2UI CCWGG 2 cut(s) 381, 425
Bst4CI ACNGT 3 cut(s) 508, 566, 665
Bst6I CTCTTC 4 cut(s) 106, 267, 522, 678
BstBAI YACGTR 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 707, 711
BstF5I GGATG 2 cut(s) 781, 994
BstH2I RGCGCY 1 cut(s) 159
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 1 cut(s) 146
BstMAI GTCTC 4 cut(s) 273, 738, 986, 1113
BstMBI GATC 1 cut(s) 143
BstMWI GCNNNNNNNGC 3 cut(s) 235, 374, 551
BstNI CCWGG 2 cut(s) 381, 425
BstNSI RCATGY 1 cut(s) 806
BstPAI GACNNNNGTC 1 cut(s) 730
BstSCI CCNGG 2 cut(s) 379, 423
BstSFI CTRYAG 1 cut(s) 372
BstSLI GKGCMC 1 cut(s) 1099
BstV1I GCAGC 2 cut(s) 386, 811
Bsu15I ATCGAT 1 cut(s) 745
BsuRI GGCC 3 cut(s) 618, 709, 1145
BsuTUI ATCGAT 1 cut(s) 745
BtsCI GGATG 2 cut(s) 781, 994
BtsI GCAGTG 1 cut(s) 332
BtsIMutI CAGTG 2 cut(s) 332, 670
BveI ACCTGC 2 cut(s) 121, 179
Cac8I GCNNGC 2 cut(s) 707, 711
CaiI CAGNNNCTG 3 cut(s) 380, 863, 1004
CciI TCATGA 2 cut(s) 922, 1187
CfoI GCGC 1 cut(s) 158
Cfr13I GGNCC 5 cut(s) 32, 329, 383, 598, 1001
ClaI ATCGAT 1 cut(s) 745
CseI GACGC 1 cut(s) 735
Csp6I GTAC 2 cut(s) 244, 902
CviAII CATG 3 cut(s) 803, 923, 1188
CviQI GTAC 2 cut(s) 244, 902
DpnI GATC 1 cut(s) 145
DpnII GATC 1 cut(s) 143
DriI GACNNNNNGTC 1 cut(s) 999
EaeI YGGCCR 1 cut(s) 616
Eam1104I CTCTTC 4 cut(s) 106, 267, 522, 678
Eam1105I GACNNNNNGTC 1 cut(s) 999
EarI CTCTTC 4 cut(s) 106, 267, 522, 678
Eco130I CCWWGG 1 cut(s) 224
Eco147I AGGCCT 1 cut(s) 709
Eco47I GGWCC 5 cut(s) 32, 329, 383, 598, 1001
Eco57I CTGAAG 3 cut(s) 702, 981, 1026
Eco88I CYCGRG 3 cut(s) 249, 953, 1110
EcoO109I RGGNCCY 1 cut(s) 1001
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 379, 423
EcoT14I CCWWGG 1 cut(s) 224
ErhI CCWWGG 1 cut(s) 224
FaeI CATG 3 cut(s) 806, 926, 1191
FalI AAGNNNNNCTT 2 cut(s) 142, 174
FaqI GGGAC 1 cut(s) 369
FatI CATG 3 cut(s) 802, 922, 1187
FblI GTMKAC 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 375, 800
FokI GGATG 2 cut(s) 788, 1001
Fsp4HI GCNGC 2 cut(s) 375, 800
FspBI CTAG 3 cut(s) 195, 348, 965
GlaI GCGC 1 cut(s) 157
GluI GCNGC 2 cut(s) 375, 800
GsuI CTGGAG 1 cut(s) 1175
HaeII RGCGCY 1 cut(s) 159
HaeIII GGCC 3 cut(s) 618, 709, 1145
HgaI GACGC 1 cut(s) 735
HhaI GCGC 1 cut(s) 158
Hin1II CATG 3 cut(s) 806, 926, 1191
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HinfI GANTC 6 cut(s) 120, 302, 512, 653, 896, 1184
Hpy166II GTNNAC 2 cut(s) 53, 332
Hpy188I TCNGA 4 cut(s) 40, 307, 772, 853
Hpy188III TCNNGA 9 cut(s) 30, 215, 415, 437, 923, 1004, 1110, 1154, 1188
Hpy8I GTNNAC 2 cut(s) 53, 332
Hpy99I CGWCG 1 cut(s) 729
HpyAV CCTTC 3 cut(s) 460, 664, 1135
HpyCH4III ACNGT 3 cut(s) 508, 566, 665
HpyCH4IV ACGT 1 cut(s) 179
HpyCH4V TGCA 6 cut(s) 183, 374, 481, 713, 799, 1073
HpyF10VI GCNNNNNNNGC 3 cut(s) 235, 374, 551
HpySE526I ACGT 1 cut(s) 179
Hsp92II CATG 3 cut(s) 806, 926, 1191
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 1 cut(s) 143
LguI GCTCTTC 2 cut(s) 106, 678
LmnI GCTCC 1 cut(s) 1173
Lsp1109I GCAGC 2 cut(s) 386, 811
LweI GCATC 1 cut(s) 722
MaeI CTAG 3 cut(s) 195, 348, 965
MaeII ACGT 1 cut(s) 179
MaeIII GTNAC 2 cut(s) 3, 350
MalI GATC 1 cut(s) 145
MboI GATC 1 cut(s) 143
MboII GAAGA 8 cut(s) 93, 284, 311, 470, 509, 695, 1019, 1150
MhlI GDGCHC 1 cut(s) 1099
MluCI AATT 5 cut(s) 657, 790, 808, 832, 1088
MlyI GAGTC 1 cut(s) 905
MmeI TCCRAC 3 cut(s) 121, 556, 576
MroXI GAANNNNTTC 1 cut(s) 1124
MseI TTAA 3 cut(s) 453, 522, 660
MspR9I CCNGG 2 cut(s) 381, 425
MvaI CCWGG 2 cut(s) 381, 425
MwoI GCNNNNNNNGC 3 cut(s) 235, 374, 551
NdeII GATC 1 cut(s) 143
NlaIII CATG 3 cut(s) 806, 926, 1191
NlaIV GGNNCC 1 cut(s) 385
NmeAIII GCCGAG 1 cut(s) 1171
NmuCI GTSAC 1 cut(s) 3
NspI RCATGY 1 cut(s) 806
PaeR7I CTCGAG 1 cut(s) 1110
PagI TCATGA 2 cut(s) 922, 1187
PceI AGGCCT 1 cut(s) 709
PciSI GCTCTTC 2 cut(s) 106, 678
PdmI GAANNNNTTC 1 cut(s) 1124
PfeI GAWTC 5 cut(s) 120, 302, 512, 653, 1184
PflFI GACNNNGTC 1 cut(s) 1034
PkrI GCNGC 2 cut(s) 376, 801
PleI GAGTC 1 cut(s) 904
PpsI GAGTC 1 cut(s) 904
Ppu21I YACGTR 1 cut(s) 180
PpuMI RGGWCCY 1 cut(s) 1001
PshAI GACNNNNGTC 1 cut(s) 730
Psp5II RGGWCCY 1 cut(s) 1001
Psp6I CCWGG 2 cut(s) 379, 423
PspGI CCWGG 2 cut(s) 379, 423
PspN4I GGNNCC 1 cut(s) 385
PspPI GGNCC 5 cut(s) 32, 329, 383, 598, 1001
PspPPI RGGWCCY 1 cut(s) 1001
PstI CTGCAG 1 cut(s) 376
PstNI CAGNNNCTG 3 cut(s) 380, 863, 1004
PsyI GACNNNGTC 1 cut(s) 1034
RsaI GTAC 2 cut(s) 245, 903
RsaNI GTAC 2 cut(s) 244, 902
SapI GCTCTTC 2 cut(s) 106, 678
SaqAI TTAA 3 cut(s) 453, 522, 660
SatI GCNGC 2 cut(s) 375, 800
Sau3AI GATC 1 cut(s) 143
Sau96I GGNCC 5 cut(s) 32, 329, 383, 598, 1001
SchI GAGTC 1 cut(s) 905
ScrFI CCNGG 2 cut(s) 381, 425
SduI GDGCHC 1 cut(s) 1099
SfaNI GCATC 1 cut(s) 722
SfcI CTRYAG 1 cut(s) 372
Sfr274I CTCGAG 1 cut(s) 1110
SinI GGWCC 5 cut(s) 32, 329, 383, 598, 1001
SlaI CTCGAG 1 cut(s) 1110
SmlI CTYRAG 3 cut(s) 881, 905, 1110
SmoI CTYRAG 3 cut(s) 881, 905, 1110
Sse9I AATT 5 cut(s) 657, 790, 808, 832, 1088
SseBI AGGCCT 1 cut(s) 709
SspMI CTAG 3 cut(s) 195, 348, 965
StuI AGGCCT 1 cut(s) 709
StyD4I CCNGG 2 cut(s) 379, 423
StyI CCWWGG 1 cut(s) 224
TaaI ACNGT 3 cut(s) 508, 566, 665
TaiI ACGT 1 cut(s) 182
TaqI TCGA 3 cut(s) 724, 745, 1111
TasI AATT 5 cut(s) 657, 790, 808, 832, 1088
TfiI GAWTC 5 cut(s) 120, 302, 512, 653, 1184
Tru1I TTAA 3 cut(s) 453, 522, 660
Tru9I TTAA 3 cut(s) 453, 522, 660
TscAI CASTG 2 cut(s) 339, 670
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 374, 799
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 5 cut(s) 5, 285, 911, 1151, 1176
TspGWI ACGGA 1 cut(s) 1076
TspRI CASTG 2 cut(s) 339, 670
Tth111I GACNNNGTC 1 cut(s) 1034
VpaK11BI GGWCC 5 cut(s) 32, 329, 383, 598, 1001
XapI RAATTY 1 cut(s) 808
XceI RCATGY 1 cut(s) 806
XhoI CTCGAG 1 cut(s) 1110
XmiI GTMKAC 1 cut(s) 52
XmnI GAANNNNTTC 1 cut(s) 1124
XspI CTAG 3 cut(s) 195, 348, 965
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.