RchiOBHm_Chr6g0301301

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0003310
Physical Location & Seq
Forward (+)
61764856 .. 61766085
1230 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ27060

Sequence Viewer

Length: 717 bp
ATGGCTGATCCTCAGGAAAGCAAGATTTTGGTAATTGGAGGCACCGGCTACATCGGCAGGTTCATCATCGGAGCTAGCGCAAAGGCTAACCATCCTAGCTTTGTTCTTGTTGGGGAAACCAATATGCCAAGAGAAAACTCATTCAAAATTTCAAGAACTTGGATGTCAGTTGTCACTGGAGATCTTCATGATGGACTTGACTGTTTGGCGAAGGTGATCAAACAGGTAGACGTGGTGATTTCAGCTGTGAATCGCATGCAGTTAGTAGATCAAACCAAGATCATTGCCGCCATTAAAGAAGCCGGTAATGTCAAGAGATTCTTCCCCTCGGAGTTTGGAAATGATGCTGATCGTGCGGAGGGGATACCCTACACATATGTCTCGAGCAACTGCTTTGCAGGCTATTTTCTACCCACATTGGCACAGCCAGGTGCTACTTCTCCGCCAAGAGACAAAGTCATCATCGTAGGGGATGGAAATCCAAAGGAAATCTTTGTCAAGGAAGAGGACATCAGCACCTACACAATCCGAGCAGTTGATGATCCAAGAACACTGAACAAGGTCCTCTACCTCAAGCCTCCCAAAAACATTTACTCATTCAATGAACTGGTTGCCTTGTGCGAGAAGAAATTTGGCAAAGCCCTCGGGAAGGTCAATGTTCCAGAGGACAAACTCCTCAACGATATTATAGGTCAGTTTAGTAAGATCCAAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

238

Amino Acids

26.16

Weight (kDa)

7.64

Isoelectric Point (pI)

21.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 119 5.1e-28 NmrA-like family
NmrA PF05368 118 - 230 4e-23 NmrA-like family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 48
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 228
AciI CCGC 3 cut(s) 288, 356, 443
AclWI GGATC 3 cut(s) 2, 536, 700
AcsI RAATTY 2 cut(s) 147, 629
AfiI CCNNNNNNNGG 1 cut(s) 649
AgsI TTSAA 3 cut(s) 145, 153, 601
AjiI CACGTC 1 cut(s) 232
AjnI CCWGG 1 cut(s) 427
AluBI AGCT 3 cut(s) 74, 99, 245
AluI AGCT 3 cut(s) 74, 99, 245
Alw26I GTCTC 2 cut(s) 385, 444
AlwI GGATC 3 cut(s) 2, 536, 700
Ama87I CYCGRG 2 cut(s) 382, 644
ApoI RAATTY 2 cut(s) 147, 629
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 562
AsuHPI GGTGA 2 cut(s) 226, 247
AsuNHI GCTAGC 1 cut(s) 74
AvaI CYCGRG 2 cut(s) 382, 644
AvaII GGWCC 1 cut(s) 562
AxyI CCTNAGG 1 cut(s) 12
BanI GGYRCC 1 cut(s) 41
BccI CCATC 3 cut(s) 99, 185, 467
BcgI CGANNNNNNTGC 2 cut(s) 625, 659
BciT130I CCWGG 1 cut(s) 429
BciVI GTATCC 1 cut(s) 357
BclI TGATCA 1 cut(s) 216
BcoDI GTCTC 2 cut(s) 385, 444
BfaI CTAG 2 cut(s) 75, 96
BfuAI ACCTGC 1 cut(s) 48
BfuI GTATCC 1 cut(s) 357
BglII AGATCT 1 cut(s) 181
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
Bme1390I CCNGG 1 cut(s) 429
Bme18I GGWCC 1 cut(s) 562
BmeT110I CYCGRG 2 cut(s) 382, 644
BmgBI CACGTC 1 cut(s) 232
BmgT120I GGNCC 1 cut(s) 562
BmiI GGNNCC 1 cut(s) 43
BmrFI CCNGG 1 cut(s) 429
BmsI GCATC 1 cut(s) 334
BmtI GCTAGC 1 cut(s) 78
BpmI CTGGAG 1 cut(s) 198
BpuEI CTTGAG 1 cut(s) 557
BsaBI GATNNNNATC 2 cut(s) 348, 477
BsaJI CCNNGG 2 cut(s) 327, 643
Bsc4I CCNNNNNNNGG 1 cut(s) 649
Bse118I RCCGGY 2 cut(s) 44, 302
Bse1I ACTGG 2 cut(s) 181, 612
Bse21I CCTNAGG 1 cut(s) 12
Bse3DI GCAATG 1 cut(s) 282
Bse8I GATNNNNATC 2 cut(s) 348, 477
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 2 cut(s) 327, 643
BseGI GGATG 3 cut(s) 91, 168, 478
BseJI GATNNNNATC 2 cut(s) 348, 477
BseLI CCNNNNNNNGG 1 cut(s) 649
BseMI GCAATG 1 cut(s) 282
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 2 cut(s) 181, 612
BseRI GAGGAG 1 cut(s) 665
BshNI GGYRCC 1 cut(s) 41
BsiHKCI CYCGRG 2 cut(s) 382, 644
BsiSI CCGG 2 cut(s) 45, 303
BslI CCNNNNNNNGG 1 cut(s) 649
BsmAI GTCTC 2 cut(s) 385, 444
BsoBI CYCGRG 2 cut(s) 382, 644
Bsp143I GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
BspACI CCGC 3 cut(s) 288, 356, 443
BspCNI CTCAG 1 cut(s) 25
BspHI TCATGA 1 cut(s) 187
BspLI GGNNCC 1 cut(s) 43
BspMI ACCTGC 1 cut(s) 48
BspOI GCTAGC 1 cut(s) 78
BspPI GGATC 3 cut(s) 2, 536, 700
BspT107I GGYRCC 1 cut(s) 41
BsrDI GCAATG 1 cut(s) 282
BsrFI RCCGGY 2 cut(s) 44, 302
BsrI ACTGG 2 cut(s) 181, 612
BssAI RCCGGY 2 cut(s) 44, 302
BssECI CCNNGG 2 cut(s) 327, 643
BssMI GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 1 cut(s) 203
Bst6I CTCTTC 1 cut(s) 498
BstC8I GCNNGC 3 cut(s) 76, 257, 400
BstDEI CTNAG 1 cut(s) 12
BstENI CCTNNNNNAGG 1 cut(s) 647
BstF5I GGATG 3 cut(s) 91, 168, 478
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 8 cut(s) 10, 184, 219, 271, 282, 352, 544, 708
BstMAI GTCTC 2 cut(s) 385, 444
BstMBI GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
BstMWI GCNNNNNNNGC 3 cut(s) 54, 353, 399
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 1 cut(s) 259
BstSCI CCNGG 1 cut(s) 427
BstX2I RGATCY 2 cut(s) 181, 705
BstYI RGATCY 2 cut(s) 181, 705
Bsu36I CCTNAGG 1 cut(s) 12
BsuI GTATCC 1 cut(s) 357
BtrI CACGTC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 91, 168, 478
BtsIMutI CAGTG 2 cut(s) 174, 551
BveI ACCTGC 1 cut(s) 48
Cac8I GCNNGC 3 cut(s) 76, 257, 400
CciI TCATGA 1 cut(s) 187
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 44, 302
Cfr13I GGNCC 1 cut(s) 562
CviAII CATG 2 cut(s) 188, 256
DdeI CTNAG 1 cut(s) 12
DpnI GATC 8 cut(s) 9, 183, 218, 270, 281, 351, 543, 707
DpnII GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
Eam1104I CTCTTC 1 cut(s) 498
EarI CTCTTC 1 cut(s) 498
EciI GGCGGA 1 cut(s) 432
Eco47I GGWCC 1 cut(s) 562
Eco81I CCTNAGG 1 cut(s) 12
Eco88I CYCGRG 2 cut(s) 382, 644
EcoNI CCTNNNNNAGG 1 cut(s) 647
EcoO109I RGGNCCY 1 cut(s) 562
EcoRII CCWGG 1 cut(s) 427
FaeI CATG 2 cut(s) 191, 259
FaiI YATR 6 cut(s) 125, 189, 257, 376, 378, 689
FalI AAGNNNNNCTT 4 cut(s) 305, 337, 476, 508
FatI CATG 2 cut(s) 187, 255
FauNDI CATATG 1 cut(s) 376
FbaI TGATCA 1 cut(s) 216
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 1 cut(s) 288
FokI GGATG 3 cut(s) 78, 175, 485
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 2 cut(s) 75, 96
GlaI GCGC 1 cut(s) 79
GluI GCNGC 1 cut(s) 288
GsuI CTGGAG 1 cut(s) 198
HapII CCGG 2 cut(s) 45, 303
HhaI GCGC 1 cut(s) 80
Hin1II CATG 2 cut(s) 191, 259
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HinfI GANTC 2 cut(s) 250, 318
HpaII CCGG 2 cut(s) 45, 303
HphI GGTGA 2 cut(s) 226, 247
Hpy166II GTNNAC 1 cut(s) 229
Hpy188I TCNGA 3 cut(s) 71, 331, 530
Hpy188III TCNNGA 7 cut(s) 14, 153, 188, 313, 382, 646, 662
Hpy8I GTNNAC 1 cut(s) 229
HpyAV CCTTC 2 cut(s) 205, 643
HpyCH4III ACNGT 1 cut(s) 203
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 2 cut(s) 259, 398
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 353, 399
HpyF3I CTNAG 1 cut(s) 12
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 2 cut(s) 191, 259
HspAI GCGC 1 cut(s) 78
Ksp22I TGATCA 1 cut(s) 216
Kzo9I GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
LmnI GCTCC 1 cut(s) 71
LweI GCATC 1 cut(s) 334
MaeI CTAG 2 cut(s) 75, 96
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 1 cut(s) 172
MalI GATC 8 cut(s) 9, 183, 218, 270, 281, 351, 543, 707
MboI GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
MboII GAAGA 4 cut(s) 176, 313, 515, 637
MflI RGATCY 2 cut(s) 181, 705
MluCI AATT 3 cut(s) 33, 147, 629
MseI TTAA 1 cut(s) 294
MspA1I CMGCKG 1 cut(s) 245
MspI CCGG 2 cut(s) 45, 303
MspR9I CCNGG 1 cut(s) 429
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 3 cut(s) 54, 353, 399
NdeI CATATG 1 cut(s) 376
NdeII GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
NheI GCTAGC 1 cut(s) 74
NlaIII CATG 2 cut(s) 191, 259
NlaIV GGNNCC 1 cut(s) 43
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 1 cut(s) 259
PaeI GCATGC 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 382
PagI TCATGA 1 cut(s) 187
PfeI GAWTC 2 cut(s) 250, 318
PflFI GACNNNGTC 1 cut(s) 455
PkrI GCNGC 1 cut(s) 289
PpuMI RGGWCCY 1 cut(s) 562
Psp5II RGGWCCY 1 cut(s) 562
Psp6I CCWGG 1 cut(s) 427
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 1 cut(s) 43
PspPI GGNCC 1 cut(s) 562
PspPPI RGGWCCY 1 cut(s) 562
PsuI RGATCY 2 cut(s) 181, 705
PsyI GACNNNGTC 1 cut(s) 455
PvuII CAGCTG 1 cut(s) 245
SaqAI TTAA 1 cut(s) 294
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 8 cut(s) 7, 181, 216, 268, 279, 349, 541, 705
Sau96I GGNCC 1 cut(s) 562
ScrFI CCNGG 1 cut(s) 429
SfaNI GCATC 1 cut(s) 334
Sfr274I CTCGAG 1 cut(s) 382
SinI GGWCC 1 cut(s) 562
SlaI CTCGAG 1 cut(s) 382
SmlI CTYRAG 2 cut(s) 382, 572
SmoI CTYRAG 2 cut(s) 382, 572
SphI GCATGC 1 cut(s) 259
Sse9I AATT 3 cut(s) 33, 147, 629
SsiI CCGC 3 cut(s) 288, 356, 443
SspMI CTAG 2 cut(s) 75, 96
StyD4I CCNGG 1 cut(s) 427
TaaI ACNGT 1 cut(s) 203
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 383
TasI AATT 3 cut(s) 33, 147, 629
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 2 cut(s) 250, 318
Tru1I TTAA 1 cut(s) 294
Tru9I TTAA 1 cut(s) 294
TscAI CASTG 2 cut(s) 181, 558
TseFI GTSAC 1 cut(s) 172
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 52, 176, 618
TspRI CASTG 2 cut(s) 181, 558
Tth111I GACNNNGTC 1 cut(s) 455
VpaK11BI GGWCC 1 cut(s) 562
XagI CCTNNNNNAGG 1 cut(s) 647
XapI RAATTY 2 cut(s) 147, 629
XceI RCATGY 1 cut(s) 259
XhoI CTCGAG 1 cut(s) 382
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 75, 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.