MD08G1029500.v1.1

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0003310
Physical Location & Seq
Reverse (-)
2133269 .. 2135397
2129 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1029500.v1.1.491

Sequence Viewer

Length: 927 bp
ATGGCTGAGAAGAGCAAGATTTTGATAATCGGAGGCACTGGCTATATCGGAAAGTTTGTCGTTGAAGCGGGCGCGAAGGCCGGGCATCGGACCTTTGCTCTGGTTAGAGAGACCACGGTCAACGACCCTGCTAAGGCTAATCTCATTGAGAAGTTCAAGAACTTGGGTGTCACTTTGCTCTATGGAGATCTTTATAACCATGAGAGTTTGGTGAAGGCGATCAAACAGGTGGACGTTGTGATATCGACGGTGGGTTTCCTGCAAACTGCAGACCAAACCAAGATCATTGCAGCCATCAAAGAAGCTGGCAATATTAAGAGATTTTTCCCGTCGGAGTTTGGAAACGATGTGGATCGAGTGAATGCAGTTGAGCCAGCCAAATCTTCGTTTGACGCCAAGGTCCAAATCCGGCGTGCTATTGAGGCTGAGGGCATCCCCCACACTTATGTCTCCAGCAACTGCTTTGCTGGCTACTTTCTGCCGTCATTGGCACAGCCAGGTGCCACTTCTCCACCCAGAGACAAAGTCACCATCTTAGGGGATGGAAATCCCAAAGCTGTCTTTAACAAGGAAGAGAACATAGGAACCTATACAATCAGGGCCGTTGATGATCCAAGAACATTGAACAAGGTCCTCTTCCTGAAGCCTCCCCAGAACATCTACTCATTCAATGAGCTTGTTGCTCTGTGGGAGAAGAAGATTGGCAAAGTCCTTGAAAAAGTCTATGTCCCAGAGGACAAACTTCTCAAGGATATTGAAGCGGCCCCAATTCCAATCAATGTGATATTAGCAATCAACCACTCAGTGTTTGTGAACGGAGATCAGACCAAGTTCGAAATCGAGCCGTCTTTTGGAGTCGAGGCTTATGAGCTCTACCCTGACGTGAAGTACACAACTGTGGACGAGTACCTTGATCAATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

309

Amino Acids

34.04

Weight (kDa)

5.92

Isoelectric Point (pI)

31.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 304 5.4e-98 NmrA-like family
Epimerase PF01370 7 - 85 1.1e-08 NAD dependent epimerase/dehydratase family
Sacchrp_dh_NADP PF03435 7 - 96 2.4e-06 Saccharopine dehydrogenase NADP binding domain
GDP_Man_Dehyd PF16363 8 - 78 3.6e-06 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 8 - 91 9.7e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 11 - 151 2.8e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 195
AasI GACNNNNNNGTC 1 cut(s) 398
AccB1I GGYRCC 1 cut(s) 500
AccII CGCG 1 cut(s) 74
AciI CCGC 2 cut(s) 68, 761
AclWI GGATC 2 cut(s) 360, 605
AcuI CTGAAG 1 cut(s) 662
AcyI GRCGYC 1 cut(s) 393
AdeI CACNNNGTG 1 cut(s) 805
AfaI GTAC 2 cut(s) 890, 908
AfiI CCNNNNNNNGG 4 cut(s) 87, 133, 537, 851
AgsI TTSAA 6 cut(s) 65, 157, 625, 670, 716, 758
AjiI CACGTC 1 cut(s) 883
AjnI CCWGG 1 cut(s) 496
AleI CACNNNNGTG 1 cut(s) 896
AluBI AGCT 4 cut(s) 305, 557, 676, 871
AluI AGCT 4 cut(s) 305, 557, 676, 871
Alw21I GWGCWC 1 cut(s) 873
Alw26I GTCTC 3 cut(s) 104, 454, 513
AlwI GGATC 2 cut(s) 360, 605
AlwNI CAGNNNCTG 1 cut(s) 459
AoxI GGCC 3 cut(s) 78, 600, 762
ApeKI GCWGC 1 cut(s) 290
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 5 cut(s) 90, 400, 600, 631, 763
AsuC2I CCSGG 1 cut(s) 82
AsuHPI GGTGA 2 cut(s) 223, 520
AsuII TTCGAA 1 cut(s) 834
AvaII GGWCC 3 cut(s) 90, 400, 631
BanI GGYRCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 873
Bbv12I GWGCWC 1 cut(s) 873
BbvCI CCTCAGC 1 cut(s) 426
BbvI GCAGC 1 cut(s) 302
BccI CCATC 3 cut(s) 302, 536, 539
BceAI ACGGC 3 cut(s) 466, 587, 829
BciT130I CCWGG 1 cut(s) 498
BclI TGATCA 1 cut(s) 913
BcnI CCSGG 1 cut(s) 82
BcoDI GTCTC 3 cut(s) 104, 454, 513
BfmI CTRYAG 1 cut(s) 267
BglII AGATCT 1 cut(s) 187
BisI GCNGC 2 cut(s) 291, 762
BlsI GCNGC 2 cut(s) 292, 763
Bme1390I CCNGG 2 cut(s) 82, 498
Bme18I GGWCC 3 cut(s) 90, 400, 631
BmgBI CACGTC 1 cut(s) 883
BmgT120I GGNCC 5 cut(s) 90, 400, 600, 631, 763
BmiI GGNNCC 3 cut(s) 502, 586, 765
BmrFI CCNGG 2 cut(s) 82, 498
BmsI GCATC 2 cut(s) 94, 441
BoxI GACNNNNGTC 1 cut(s) 116
BpmI CTGGAG 1 cut(s) 436
Bpu10I CCTNAGC 2 cut(s) 132, 426
Bpu14I TTCGAA 1 cut(s) 834
BpuEI CTTGAG 1 cut(s) 731
BpuMI CCSGG 1 cut(s) 82
BsaBI GATNNNNATC 2 cut(s) 351, 546
BsaHI GRCGYC 1 cut(s) 393
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 114, 396
Bsc4I CCNNNNNNNGG 4 cut(s) 87, 133, 537, 851
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 1 cut(s) 285
Bse8I GATNNNNATC 2 cut(s) 351, 546
BseBI CCWGG 1 cut(s) 498
BseDI CCNNGG 2 cut(s) 114, 396
BseGI GGATG 2 cut(s) 432, 547
BseJI GATNNNNATC 2 cut(s) 351, 546
BseLI CCNNNNNNNGG 4 cut(s) 87, 133, 537, 851
BseMI GCAATG 1 cut(s) 285
BseMII CTCAG 2 cut(s) 417, 816
BseNI ACTGG 1 cut(s) 43
BseXI GCAGC 1 cut(s) 302
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 3 cut(s) 80, 602, 764
BshNI GGYRCC 1 cut(s) 500
BsiHKAI GWGCWC 1 cut(s) 873
BsiSI CCGG 2 cut(s) 81, 409
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 4 cut(s) 87, 133, 537, 851
BsmAI GTCTC 3 cut(s) 104, 454, 513
BsmFI GGGAC 1 cut(s) 713
BsmI GAATGC 1 cut(s) 367
BsnI GGCC 3 cut(s) 80, 602, 764
Bso31I GGTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 834
Bsp1286I GDGCHC 1 cut(s) 873
Bsp143I GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
BspACI CCGC 2 cut(s) 68, 761
BspANI GGCC 3 cut(s) 80, 602, 764
BspCNI CTCAG 2 cut(s) 418, 815
BspFNI CGCG 1 cut(s) 74
BspLI GGNNCC 3 cut(s) 502, 586, 765
BspMAI CTGCAG 1 cut(s) 271
BspPI GGATC 2 cut(s) 360, 605
BspQI GCTCTTC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 834
BspT107I GGYRCC 1 cut(s) 500
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 1 cut(s) 285
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 2 cut(s) 114, 396
BssMI GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
BssNI GRCGYC 1 cut(s) 393
BssT1I CCWWGG 1 cut(s) 396
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 3 cut(s) 118, 250, 898
Bst6I CTCTTC 3 cut(s) 5, 567, 641
BstACI GRCGYC 1 cut(s) 393
BstBI TTCGAA 1 cut(s) 834
BstC8I GCNNGC 5 cut(s) 70, 307, 375, 414, 469
BstDEI CTNAG 5 cut(s) 6, 132, 426, 535, 802
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 2 cut(s) 432, 547
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 7 cut(s) 190, 222, 285, 355, 613, 823, 916
BstMAI GTCTC 3 cut(s) 104, 454, 513
BstMBI GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
BstMWI GCNNNNNNNGC 2 cut(s) 422, 468
BstNI CCWGG 1 cut(s) 498
BstPAI GACNNNNGTC 1 cut(s) 116
BstSCI CCNGG 2 cut(s) 80, 496
BstSFI CTRYAG 1 cut(s) 267
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 1 cut(s) 302
BstX2I RGATCY 1 cut(s) 187
BstYI RGATCY 1 cut(s) 187
BsuRI GGCC 3 cut(s) 80, 602, 764
BtgI CCRYGG 1 cut(s) 114
BtrI CACGTC 1 cut(s) 883
BtsCI GGATG 2 cut(s) 432, 547
BtsIMutI CAGTG 2 cut(s) 36, 810
Cac8I GCNNGC 5 cut(s) 70, 307, 375, 414, 469
CaiI CAGNNNCTG 1 cut(s) 459
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 5 cut(s) 90, 400, 600, 631, 763
CseI GACGC 1 cut(s) 401
Csp6I GTAC 2 cut(s) 889, 907
CviAII CATG 1 cut(s) 200
CviQI GTAC 2 cut(s) 889, 907
DdeI CTNAG 5 cut(s) 6, 132, 426, 535, 802
DpnI GATC 7 cut(s) 189, 221, 284, 354, 612, 822, 915
DpnII GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
DraIII CACNNNGTG 1 cut(s) 805
DrdI GACNNNNNNGTC 1 cut(s) 398
DseDI GACNNNNNNGTC 1 cut(s) 398
Eam1104I CTCTTC 3 cut(s) 5, 567, 641
EarI CTCTTC 3 cut(s) 5, 567, 641
Ecl136II GAGCTC 1 cut(s) 871
Eco130I CCWWGG 1 cut(s) 396
Eco24I GRGCYC 1 cut(s) 873
Eco31I GGTCTC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 243
Eco47I GGWCC 3 cut(s) 90, 400, 631
Eco53kI GAGCTC 1 cut(s) 871
Eco57I CTGAAG 1 cut(s) 662
EcoICRI GAGCTC 1 cut(s) 871
EcoO109I RGGNCCY 1 cut(s) 631
EcoRII CCWGG 1 cut(s) 496
EcoRV GATATC 1 cut(s) 243
EcoT14I CCWWGG 1 cut(s) 396
EcoT38I GRGCYC 1 cut(s) 873
ErhI CCWWGG 1 cut(s) 396
FaeI CATG 1 cut(s) 203
FaiI YATR 9 cut(s) 45, 183, 195, 201, 447, 581, 591, 726, 867
FalI AAGNNNNNCTT 2 cut(s) 620, 652
FaqI GGGAC 1 cut(s) 713
FatI CATG 1 cut(s) 199
FauI CCCGC 1 cut(s) 61
FbaI TGATCA 1 cut(s) 913
Fnu4HI GCNGC 2 cut(s) 291, 762
FokI GGATG 2 cut(s) 419, 554
FriOI GRGCYC 1 cut(s) 873
Fsp4HI GCNGC 2 cut(s) 291, 762
GlaI GCGC 1 cut(s) 73
GluI GCNGC 2 cut(s) 291, 762
GsuI CTGGAG 1 cut(s) 436
HaeIII GGCC 3 cut(s) 80, 602, 764
HapII CCGG 2 cut(s) 81, 409
HgaI GACGC 1 cut(s) 401
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 1 cut(s) 203
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HinfI GANTC 1 cut(s) 855
HpaII CCGG 2 cut(s) 81, 409
HphI GGTGA 2 cut(s) 223, 520
Hpy166II GTNNAC 5 cut(s) 121, 232, 814, 891, 901
Hpy188I TCNGA 5 cut(s) 32, 50, 90, 334, 825
Hpy188III TCNNGA 2 cut(s) 157, 640
Hpy8I GTNNAC 5 cut(s) 121, 232, 814, 891, 901
Hpy99I CGWCG 2 cut(s) 250, 334
HpyAV CCTTC 2 cut(s) 70, 208
HpyCH4III ACNGT 3 cut(s) 118, 250, 898
HpyCH4IV ACGT 2 cut(s) 234, 882
HpyCH4V TGCA 4 cut(s) 262, 269, 290, 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 468
HpyF3I CTNAG 5 cut(s) 6, 132, 426, 535, 802
HpySE526I ACGT 2 cut(s) 234, 882
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 1 cut(s) 203
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 913
Kzo9I GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
LguI GCTCTTC 1 cut(s) 5
Lsp1109I GCAGC 1 cut(s) 302
LweI GCATC 2 cut(s) 94, 441
MaeII ACGT 2 cut(s) 234, 882
MaeIII GTNAC 2 cut(s) 169, 526
MalI GATC 7 cut(s) 189, 221, 284, 354, 612, 822, 915
MboI GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
MboII GAAGA 6 cut(s) 22, 375, 584, 628, 706, 709
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 1 cut(s) 873
MluCI AATT 2 cut(s) 768, 917
MlyI GAGTC 1 cut(s) 864
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 7 cut(s) 26, 415, 421, 644, 657, 727, 853
MseI TTAA 2 cut(s) 315, 564
MslI CAYNNNNRTG 2 cut(s) 444, 896
MspI CCGG 2 cut(s) 81, 409
MspR9I CCNGG 2 cut(s) 82, 498
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 2 cut(s) 422, 468
NciI CCSGG 1 cut(s) 82
NdeII GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
NlaIII CATG 1 cut(s) 203
NlaIV GGNNCC 3 cut(s) 502, 586, 765
NmuCI GTSAC 2 cut(s) 169, 526
NspV TTCGAA 1 cut(s) 834
OliI CACNNNNGTG 1 cut(s) 896
PciSI GCTCTTC 1 cut(s) 5
PctI GAATGC 1 cut(s) 367
PflFI GACNNNGTC 1 cut(s) 524
PkrI GCNGC 2 cut(s) 292, 763
PleI GAGTC 1 cut(s) 863
PpsI GAGTC 1 cut(s) 863
PpuMI RGGWCCY 1 cut(s) 631
PshAI GACNNNNGTC 1 cut(s) 116
PsiI TTATAA 1 cut(s) 195
Psp124BI GAGCTC 1 cut(s) 873
Psp5II RGGWCCY 1 cut(s) 631
Psp6I CCWGG 1 cut(s) 496
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 502, 586, 765
PspPI GGNCC 5 cut(s) 90, 400, 600, 631, 763
PspPPI RGGWCCY 1 cut(s) 631
PstI CTGCAG 1 cut(s) 271
PstNI CAGNNNCTG 1 cut(s) 459
PsuI RGATCY 1 cut(s) 187
PsyI GACNNNGTC 1 cut(s) 524
RsaI GTAC 2 cut(s) 890, 908
RsaNI GTAC 2 cut(s) 889, 907
RseI CAYNNNNRTG 2 cut(s) 444, 896
SacI GAGCTC 1 cut(s) 873
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 2 cut(s) 315, 564
SatI GCNGC 2 cut(s) 291, 762
Sau3AI GATC 7 cut(s) 187, 219, 282, 352, 610, 820, 913
Sau96I GGNCC 5 cut(s) 90, 400, 600, 631, 763
SchI GAGTC 1 cut(s) 864
ScrFI CCNGG 2 cut(s) 82, 498
SduI GDGCHC 1 cut(s) 873
SfaNI GCATC 2 cut(s) 94, 441
SfcI CTRYAG 1 cut(s) 267
SfuI TTCGAA 1 cut(s) 834
SinI GGWCC 3 cut(s) 90, 400, 631
SmiMI CAYNNNNRTG 2 cut(s) 444, 896
SmlI CTYRAG 1 cut(s) 746
SmoI CTYRAG 1 cut(s) 746
Sse9I AATT 2 cut(s) 768, 917
SsiI CCGC 2 cut(s) 68, 761
SspI AATATT 1 cut(s) 313
SstI GAGCTC 1 cut(s) 873
StyD4I CCNGG 2 cut(s) 80, 496
StyI CCWWGG 1 cut(s) 396
TaaI ACNGT 3 cut(s) 118, 250, 898
TaiI ACGT 2 cut(s) 237, 885
TaqI TCGA 5 cut(s) 245, 355, 834, 840, 858
TasI AATT 2 cut(s) 768, 917
TatI WGTACW 1 cut(s) 888
TauI GCSGC 1 cut(s) 764
Tru1I TTAA 2 cut(s) 315, 564
Tru9I TTAA 2 cut(s) 315, 564
TscAI CASTG 2 cut(s) 43, 810
TseFI GTSAC 2 cut(s) 169, 526
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 169, 526
TspGWI ACGGA 1 cut(s) 831
TspRI CASTG 2 cut(s) 43, 810
Tth111I GACNNNGTC 1 cut(s) 524
VpaK11BI GGWCC 3 cut(s) 90, 400, 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.