FvH4_2g39620

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003310
Physical Location & Seq
Reverse (-)
28410634 .. 28412322
1689 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39620.t1

Sequence Viewer

Length: 930 bp
ATGGCTTCTGAGAAAAGCAAGATCTTGATCATCGGAGGCACCGGCTACATCGGCAAGTTCATTGTCGAAGCGAGCGCAAAGGCTAACCATCCCACCTTTCTTCTTGTTAGGGAGTCCACGGTAAATGACCCTGCCAAGGGAGCTCTCATTCAGAAGTTCAAGAACTTGGGGGTCACTTTGCTCTATGGGGATCTTTTTGATCATGAGAGCTTGGTGAAAGCCATCAAACAGGTCGATGTGGTGATATCAACAGTGGGTAACTTTCAGCTAGCAGATCAAACCAAGATCATTGCTGCCATCAAAGAAGCTGGTAATGTTAAGAGATTCTTCCCCTCGGAATTTGGAAACGATGTGGATCGTGTACATGCAGTTGAGCCAGCTAAATCTGCCTTTGCAATCAAAGCCCAAATCCGCCGCGCTATAGAAGCTGAGGGGATACCCTACACTTATGTCTCCAGCAACTGCTTTGCAGGCTATTTTCTGCCCACATTGGTACAACTAGGAGCTACTTCTCCACCCAGAGACAAAGTCACTATCCTAGGGGATGGAAATCCAAAGGCAATCTTTAACAAGGAAGATGACATTGGCACCTACACGATCCGAGCGGTTGATGATCCAAGAACATTGAACAAGGTCCTCTACATCAAGCCTCCCAAGAACACTTACTCCTTTAATGAACTCGTTGCCTTGTGGGAGAAGAAAATTGGAAAAGTTCTTACGAAGGTCTATGTTCCAGAGGACAAACTCCTCAACGACATTCTAGAGGCTCCAATTCCGATCAATGTGATATTGGCAATCAACCACTCTATTTTTGTGAAAGGAGATCACACCAACTTCGAAATTGAGCCGTCTTTCGGAGTCGAAGCCTCCGAGCTGTACCCGGATGTTAAGTACACCACCGTGGAGGAGTATCTCGACCAGTTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

310

Amino Acids

34.15

Weight (kDa)

6.02

Isoelectric Point (pI)

21.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 305 5.7e-100 NmrA-like family
Epimerase PF01370 8 - 86 7.7e-08 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 9 - 92 8.6e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 152 7.3e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 38, 587
AccBSI CCGCTC 1 cut(s) 605
AccII CGCG 1 cut(s) 417
AciI CCGC 3 cut(s) 412, 415, 605
AclWI GGATC 4 cut(s) 198, 363, 592, 608
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 4 cut(s) 363, 495, 878, 893
AfiI CCNNNNNNNGG 3 cut(s) 136, 137, 854
AgsI TTSAA 2 cut(s) 160, 628
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AleI CACNNNNGTG 1 cut(s) 899
AloI GAACNNNNNNTCC 2 cut(s) 650, 682
AluBI AGCT 8 cut(s) 143, 210, 268, 308, 380, 428, 506, 874
AluI AGCT 8 cut(s) 143, 210, 268, 308, 380, 428, 506, 874
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 2 cut(s) 457, 516
AlwI GGATC 4 cut(s) 198, 363, 592, 608
AlwNI CAGNNNCTG 1 cut(s) 462
ApeKI GCWGC 1 cut(s) 293
ApoI RAATTY 1 cut(s) 338
AspA2I CCTAGG 1 cut(s) 538
AspLEI GCGC 2 cut(s) 77, 419
AspS9I GGNCC 1 cut(s) 634
AsuC2I CCSGG 1 cut(s) 881
AsuHPI GGTGA 2 cut(s) 226, 253
AsuII TTCGAA 1 cut(s) 837
AsuNHI GCTAGC 1 cut(s) 268
AvaII GGWCC 1 cut(s) 634
AvrII CCTAGG 1 cut(s) 538
BanI GGYRCC 2 cut(s) 38, 587
BanII GRGCYC 1 cut(s) 145
Bbv12I GWGCWC 1 cut(s) 145
BbvCI CCTCAGC 1 cut(s) 429
BbvI GCAGC 1 cut(s) 280
BccI CCATC 4 cut(s) 96, 230, 305, 539
BceAI ACGGC 1 cut(s) 832
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 2 cut(s) 27, 199
BcnI CCSGG 1 cut(s) 881
BcoDI GTCTC 2 cut(s) 457, 516
BfaI CTAG 4 cut(s) 269, 500, 539, 761
BfmI CTRYAG 1 cut(s) 420
BfuI GTATCC 1 cut(s) 429
BglII AGATCT 1 cut(s) 21
BisI GCNGC 2 cut(s) 294, 415
BlnI CCTAGG 1 cut(s) 538
BlsI GCNGC 2 cut(s) 295, 416
Bme1390I CCNGG 1 cut(s) 881
Bme18I GGWCC 1 cut(s) 634
BmgT120I GGNCC 1 cut(s) 634
BmiI GGNNCC 3 cut(s) 40, 589, 768
BmrFI CCNGG 1 cut(s) 881
BmtI GCTAGC 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 1 cut(s) 429
Bpu14I TTCGAA 1 cut(s) 837
BpuMI CCSGG 1 cut(s) 881
BsaBI GATNNNNATC 3 cut(s) 26, 354, 549
BsaJI CCNNGG 5 cut(s) 117, 135, 333, 538, 900
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 3 cut(s) 136, 137, 854
Bse118I RCCGGY 1 cut(s) 41
Bse1I ACTGG 1 cut(s) 919
Bse3DI GCAATG 1 cut(s) 288
Bse8I GATNNNNATC 3 cut(s) 26, 354, 549
BseDI CCNNGG 5 cut(s) 117, 135, 333, 538, 900
BseGI GGATG 3 cut(s) 88, 550, 889
BseJI GATNNNNATC 3 cut(s) 26, 354, 549
BseLI CCNNNNNNNGG 3 cut(s) 136, 137, 854
BseMI GCAATG 1 cut(s) 288
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 1 cut(s) 919
BseRI GAGGAG 2 cut(s) 737, 920
BseXI GCAGC 1 cut(s) 280
Bsh1236I CGCG 1 cut(s) 417
BshNI GGYRCC 2 cut(s) 38, 587
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 2 cut(s) 42, 881
BslI CCNNNNNNNGG 3 cut(s) 136, 137, 854
BsmAI GTCTC 2 cut(s) 457, 516
Bsp119I TTCGAA 1 cut(s) 837
Bsp1286I GDGCHC 1 cut(s) 145
Bsp1407I TGTACA 1 cut(s) 361
BspACI CCGC 3 cut(s) 412, 415, 605
BspCNI CTCAG 1 cut(s) 421
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 40, 589, 768
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 4 cut(s) 198, 363, 592, 608
BspT104I TTCGAA 1 cut(s) 837
BspT107I GGYRCC 2 cut(s) 38, 587
BsrBI CCGCTC 1 cut(s) 605
BsrDI GCAATG 1 cut(s) 288
BsrFI RCCGGY 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 1 cut(s) 919
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 5 cut(s) 117, 135, 333, 538, 900
BssT1I CCWWGG 2 cut(s) 135, 538
Bst4CI ACNGT 3 cut(s) 121, 253, 901
BstAUI TGTACA 1 cut(s) 361
BstBI TTCGAA 1 cut(s) 837
BstC8I GCNNGC 4 cut(s) 73, 270, 378, 472
BstDEI CTNAG 2 cut(s) 9, 429
BstDSI CCRYGG 2 cut(s) 117, 900
BstF5I GGATG 3 cut(s) 88, 550, 889
BstFNI CGCG 1 cut(s) 417
BstHHI GCGC 2 cut(s) 77, 419
BstMAI GTCTC 2 cut(s) 457, 516
BstMWI GCNNNNNNNGC 6 cut(s) 51, 140, 386, 401, 425, 471
BstNSI RCATGY 1 cut(s) 368
BstSCI CCNGG 1 cut(s) 879
BstSFI CTRYAG 1 cut(s) 420
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 280
BstX2I RGATCY 2 cut(s) 21, 190
BstYI RGATCY 2 cut(s) 21, 190
BsuI GTATCC 1 cut(s) 429
BtgI CCRYGG 2 cut(s) 117, 900
BtsCI GGATG 3 cut(s) 88, 550, 889
BtsIMutI CAGTG 1 cut(s) 258
Cac8I GCNNGC 4 cut(s) 73, 270, 378, 472
CaiI CAGNNNCTG 1 cut(s) 462
CciI TCATGA 1 cut(s) 202
CfoI GCGC 2 cut(s) 77, 419
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 1 cut(s) 634
Csp6I GTAC 4 cut(s) 362, 494, 877, 892
CviAII CATG 2 cut(s) 203, 365
CviQI GTAC 4 cut(s) 362, 494, 877, 892
DdeI CTNAG 2 cut(s) 9, 429
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 2 cut(s) 135, 538
Eco24I GRGCYC 1 cut(s) 145
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 634
Eco53kI GAGCTC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 634
EcoRV GATATC 1 cut(s) 246
EcoT14I CCWWGG 2 cut(s) 135, 538
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 2 cut(s) 135, 538
FaeI CATG 2 cut(s) 206, 368
FaiI YATR 6 cut(s) 186, 204, 366, 422, 450, 729
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 548, 580
FatI CATG 2 cut(s) 202, 364
FbaI TGATCA 2 cut(s) 27, 199
Fnu4HI GCNGC 2 cut(s) 294, 415
FokI GGATG 3 cut(s) 75, 557, 896
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 2 cut(s) 294, 415
FspBI CTAG 4 cut(s) 269, 500, 539, 761
GlaI GCGC 2 cut(s) 76, 418
GluI GCNGC 2 cut(s) 294, 415
GsuI CTGGAG 1 cut(s) 439
HapII CCGG 2 cut(s) 42, 881
HhaI GCGC 2 cut(s) 77, 419
Hin1II CATG 2 cut(s) 206, 368
Hin6I GCGC 2 cut(s) 75, 417
HinP1I GCGC 2 cut(s) 75, 417
HinfI GANTC 3 cut(s) 113, 324, 858
HpaII CCGG 2 cut(s) 42, 881
HphI GGTGA 2 cut(s) 226, 253
Hpy166II GTNNAC 3 cut(s) 117, 362, 894
Hpy188I TCNGA 8 cut(s) 10, 35, 153, 337, 602, 777, 857, 871
Hpy188III TCNNGA 6 cut(s) 25, 160, 203, 734, 761, 914
Hpy8I GTNNAC 3 cut(s) 117, 362, 894
HpyAV CCTTC 1 cut(s) 715
HpyCH4III ACNGT 3 cut(s) 121, 253, 901
HpyCH4V TGCA 3 cut(s) 368, 395, 470
HpyF10VI GCNNNNNNNGC 6 cut(s) 51, 140, 386, 401, 425, 471
HpyF3I CTNAG 2 cut(s) 9, 429
Hsp92II CATG 2 cut(s) 206, 368
HspAI GCGC 2 cut(s) 75, 417
Ksp22I TGATCA 2 cut(s) 27, 199
LmnI GCTCC 3 cut(s) 140, 503, 772
Lsp1109I GCAGC 1 cut(s) 280
MaeI CTAG 4 cut(s) 269, 500, 539, 761
MaeIII GTNAC 3 cut(s) 172, 257, 529
MbiI CCGCTC 1 cut(s) 605
MboII GAAGA 4 cut(s) 92, 319, 587, 709
MflI RGATCY 2 cut(s) 21, 190
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 4 cut(s) 338, 702, 771, 840
MlyI GAGTC 2 cut(s) 122, 867
MseI TTAA 5 cut(s) 318, 567, 672, 888, 928
MslI CAYNNNNRTG 1 cut(s) 899
MspI CCGG 2 cut(s) 42, 881
MspR9I CCNGG 1 cut(s) 881
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 6 cut(s) 51, 140, 386, 401, 425, 471
NciI CCSGG 1 cut(s) 881
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 2 cut(s) 206, 368
NlaIV GGNNCC 3 cut(s) 40, 589, 768
NmuCI GTSAC 2 cut(s) 172, 529
NspI RCATGY 1 cut(s) 368
NspV TTCGAA 1 cut(s) 837
OliI CACNNNNGTG 1 cut(s) 899
PagI TCATGA 1 cut(s) 202
PcsI WCGNNNNNNNCGW 1 cut(s) 867
PfeI GAWTC 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 2 cut(s) 295, 416
PleI GAGTC 2 cut(s) 121, 866
PpsI GAGTC 2 cut(s) 121, 866
PpuMI RGGWCCY 1 cut(s) 634
Psp124BI GAGCTC 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 40, 589, 768
PspPI GGNCC 1 cut(s) 634
PspPPI RGGWCCY 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 462
PsuI RGATCY 2 cut(s) 21, 190
PsyI GACNNNGTC 1 cut(s) 527
RsaI GTAC 4 cut(s) 363, 495, 878, 893
RsaNI GTAC 4 cut(s) 362, 494, 877, 892
RseI CAYNNNNRTG 1 cut(s) 899
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 5 cut(s) 318, 567, 672, 888, 928
SatI GCNGC 2 cut(s) 294, 415
Sau96I GGNCC 1 cut(s) 634
SchI GAGTC 2 cut(s) 122, 867
ScrFI CCNGG 1 cut(s) 881
SduI GDGCHC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 420
SfuI TTCGAA 1 cut(s) 837
SinI GGWCC 1 cut(s) 634
SmiMI CAYNNNNRTG 1 cut(s) 899
Sse9I AATT 4 cut(s) 338, 702, 771, 840
SsiI CCGC 3 cut(s) 412, 415, 605
SspMI CTAG 4 cut(s) 269, 500, 539, 761
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 879
StyI CCWWGG 2 cut(s) 135, 538
TaaI ACNGT 3 cut(s) 121, 253, 901
TaqI TCGA 5 cut(s) 66, 234, 837, 861, 915
TasI AATT 4 cut(s) 338, 702, 771, 840
TatI WGTACW 2 cut(s) 361, 891
TauI GCSGC 1 cut(s) 417
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 5 cut(s) 318, 567, 672, 888, 928
Tru9I TTAA 5 cut(s) 318, 567, 672, 888, 928
TscAI CASTG 1 cut(s) 258
TseFI GTSAC 2 cut(s) 172, 529
TseI GCWGC 1 cut(s) 293
Tsp45I GTSAC 2 cut(s) 172, 529
TspDTI ATGAA 2 cut(s) 49, 690
TspRI CASTG 1 cut(s) 258
Tth111I GACNNNGTC 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 634
XapI RAATTY 1 cut(s) 338
XbaI TCTAGA 1 cut(s) 760
XceI RCATGY 1 cut(s) 368
XmaJI CCTAGG 1 cut(s) 538
XspI CTAG 4 cut(s) 269, 500, 539, 761
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.