Rh6DG416500

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0003310
Physical Location & Seq
Forward (+)
60023189 .. 60025199
2011 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG416500.1

Sequence Viewer

Length: 783 bp
ATGGCCGCTCAGAAAAGCAAGATCTTGATCATCGGAGGCACCGGCTATATTGGCAAGTTCATCGTCGAAGCGAGCGCAAAGGCTAGCCATCCCACCTTTCTTCTTGTTAGGGAGACCACAGTCAATGACCCTGCCAAGGGAGCTCTCATTCAGAACTTCAAGAACTTGGGGGTCACTTTGCTCTATGGGGATCTTTATGATCATGAGAGCTTGGTGAAGGCGATCAAACAGGTCGATGTGGTGATATCAGCGGTGGGCCACATGCAGATAGCAGATCAAACCAAGATCATTGCGGCCATCAAAGAAGCTGGTAATGTCAAGAGATTCTTCCCCTCGGAATTTGGAAACGATGTGGATCGTGTACATGCGGTTGAGCCAGCAAAATCTGCATTTGCAATGAAAGCCCAAATCCGCCGCGCTATAGAGGCTGAGGGGATACCTTACACTTACGTCTCCAGCAACTGCTTTGCAGCCTATTTTCTGCCCACATTGGCACAGCCAGGAGCCACTTCTCCACCCAGAGACAAAGTCATCATCTTAGGGGATGGAAATGCAAAGGCAATCTTTAACAAGGAAGACGACATTGGCACCTATACGATCCGAGCGGTTGATGATCCAAGAACACTGAACAAGGTCCTCTACCTCAAGCCTCCCAGAAACATTTACTCCTTCAATGAACTCGTTGCCTTGTGGGAGAAGAAAATTGGCAAAGTTCTTGAGAAGGTCTATGTTCCAGAGGACAAACTCCTCAGCGACATTCTTGGTCAGTTATATATATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

260

Amino Acids

28.58

Weight (kDa)

8.91

Isoelectric Point (pI)

23.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 4 - 253 2.6e-84 NmrA-like family
Epimerase PF01370 8 - 88 4.1e-08 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 12 - 152 4e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 781
AccB1I GGYRCC 2 cut(s) 38, 587
AccBSI CCGCTC 2 cut(s) 8, 605
AccII CGCG 1 cut(s) 417
AciI CCGC 7 cut(s) 6, 251, 293, 368, 412, 415, 605
AclWI GGATC 4 cut(s) 198, 363, 592, 608
AcoI YGGCCR 2 cut(s) 3, 294
AcsI RAATTY 1 cut(s) 338
AfaI GTAC 1 cut(s) 363
AfiI CCNNNNNNNGG 2 cut(s) 136, 137
AgsI TTSAA 2 cut(s) 160, 673
AjnI CCWGG 1 cut(s) 499
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AluBI AGCT 3 cut(s) 143, 210, 308
AluI AGCT 3 cut(s) 143, 210, 308
Alw21I GWGCWC 1 cut(s) 145
Alw26I GTCTC 3 cut(s) 107, 457, 516
AlwI GGATC 4 cut(s) 198, 363, 592, 608
AlwNI CAGNNNCTG 1 cut(s) 462
AoxI GGCC 3 cut(s) 3, 256, 294
ApeKI GCWGC 1 cut(s) 470
ApoI RAATTY 1 cut(s) 338
AspLEI GCGC 2 cut(s) 77, 419
AspS9I GGNCC 2 cut(s) 256, 634
AsuHPI GGTGA 2 cut(s) 226, 253
AsuNHI GCTAGC 1 cut(s) 83
AvaII GGWCC 1 cut(s) 634
BanI GGYRCC 2 cut(s) 38, 587
BanII GRGCYC 1 cut(s) 145
BbsI GAAGAC 1 cut(s) 582
Bbv12I GWGCWC 1 cut(s) 145
BbvCI CCTCAGC 2 cut(s) 429, 749
BbvI GCAGC 1 cut(s) 482
BccI CCATC 3 cut(s) 96, 305, 539
BcgI CGANNNNNNTGC 2 cut(s) 43, 77
BciT130I CCWGG 1 cut(s) 501
BciVI GTATCC 1 cut(s) 429
BclI TGATCA 2 cut(s) 27, 199
BcoDI GTCTC 3 cut(s) 107, 457, 516
BfaI CTAG 1 cut(s) 84
BfmI CTRYAG 1 cut(s) 420
BfuI GTATCC 1 cut(s) 429
BglII AGATCT 1 cut(s) 21
BisI GCNGC 4 cut(s) 6, 294, 415, 471
BlsI GCNGC 4 cut(s) 7, 295, 416, 472
Bme1390I CCNGG 1 cut(s) 501
Bme18I GGWCC 1 cut(s) 634
BmgT120I GGNCC 2 cut(s) 256, 634
BmiI GGNNCC 3 cut(s) 40, 505, 589
BmrFI CCNGG 1 cut(s) 501
BmtI GCTAGC 1 cut(s) 87
BoxI GACNNNNGTC 1 cut(s) 119
BpiI GAAGAC 1 cut(s) 582
BpmI CTGGAG 1 cut(s) 439
Bpu10I CCTNAGC 2 cut(s) 429, 749
BpuEI CTTGAG 2 cut(s) 629, 737
BsaBI GATNNNNATC 2 cut(s) 26, 354
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 2 cut(s) 135, 333
BsaXI ACNNNNNCTCC 2 cut(s) 650, 680
Bsc4I CCNNNNNNNGG 2 cut(s) 136, 137
Bse118I RCCGGY 1 cut(s) 41
Bse3DI GCAATG 2 cut(s) 288, 402
Bse8I GATNNNNATC 2 cut(s) 26, 354
BseBI CCWGG 1 cut(s) 501
BseDI CCNNGG 2 cut(s) 135, 333
BseGI GGATG 2 cut(s) 88, 550
BseJI GATNNNNATC 2 cut(s) 26, 354
BseLI CCNNNNNNNGG 2 cut(s) 136, 137
BseMI GCAATG 2 cut(s) 288, 402
BseMII CTCAG 3 cut(s) 23, 420, 763
BseRI GAGGAG 1 cut(s) 737
BseXI GCAGC 1 cut(s) 482
Bsh1236I CGCG 1 cut(s) 417
BshFI GGCC 3 cut(s) 5, 258, 296
BshNI GGYRCC 2 cut(s) 38, 587
BsiHKAI GWGCWC 1 cut(s) 145
BsiSI CCGG 1 cut(s) 42
BslI CCNNNNNNNGG 2 cut(s) 136, 137
BsmAI GTCTC 3 cut(s) 107, 457, 516
BsmBI CGTCTC 1 cut(s) 457
BsnI GGCC 3 cut(s) 5, 258, 296
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 1 cut(s) 145
Bsp1407I TGTACA 1 cut(s) 361
BspACI CCGC 7 cut(s) 6, 251, 293, 368, 412, 415, 605
BspANI GGCC 3 cut(s) 5, 258, 296
BspCNI CTCAG 3 cut(s) 22, 421, 762
BspFNI CGCG 1 cut(s) 417
BspHI TCATGA 1 cut(s) 202
BspLI GGNNCC 3 cut(s) 40, 505, 589
BspOI GCTAGC 1 cut(s) 87
BspPI GGATC 4 cut(s) 198, 363, 592, 608
BspT107I GGYRCC 2 cut(s) 38, 587
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 2 cut(s) 8, 605
BsrDI GCAATG 2 cut(s) 288, 402
BsrFI RCCGGY 1 cut(s) 41
BsrGI TGTACA 1 cut(s) 361
BssAI RCCGGY 1 cut(s) 41
BssECI CCNNGG 2 cut(s) 135, 333
BssT1I CCWWGG 1 cut(s) 135
Bst2UI CCWGG 1 cut(s) 501
Bst4CI ACNGT 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 361
BstC8I GCNNGC 3 cut(s) 73, 85, 378
BstDEI CTNAG 4 cut(s) 9, 429, 538, 749
BstF5I GGATG 2 cut(s) 88, 550
BstFNI CGCG 1 cut(s) 417
BstHHI GCGC 2 cut(s) 77, 419
BstMAI GTCTC 3 cut(s) 107, 457, 516
BstMWI GCNNNNNNNGC 5 cut(s) 51, 140, 386, 401, 425
BstNI CCWGG 1 cut(s) 501
BstNSI RCATGY 2 cut(s) 265, 368
BstPAI GACNNNNGTC 1 cut(s) 119
BstSCI CCNGG 1 cut(s) 499
BstSFI CTRYAG 1 cut(s) 420
BstUI CGCG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 482
BstV2I GAAGAC 1 cut(s) 582
BstX2I RGATCY 2 cut(s) 21, 190
BstYI RGATCY 2 cut(s) 21, 190
BsuI GTATCC 1 cut(s) 429
BsuRI GGCC 3 cut(s) 5, 258, 296
BtsCI GGATG 2 cut(s) 88, 550
BtsIMutI CAGTG 1 cut(s) 623
Cac8I GCNNGC 3 cut(s) 73, 85, 378
CaiI CAGNNNCTG 1 cut(s) 462
CciI TCATGA 1 cut(s) 202
CfoI GCGC 2 cut(s) 77, 419
Cfr10I RCCGGY 1 cut(s) 41
Cfr13I GGNCC 2 cut(s) 256, 634
Csp6I GTAC 1 cut(s) 362
CviAII CATG 3 cut(s) 203, 262, 365
CviQI GTAC 1 cut(s) 362
DdeI CTNAG 4 cut(s) 9, 429, 538, 749
EaeI YGGCCR 2 cut(s) 3, 294
EciI GGCGGA 1 cut(s) 401
Ecl136II GAGCTC 1 cut(s) 143
Eco130I CCWWGG 1 cut(s) 135
Eco24I GRGCYC 1 cut(s) 145
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 246
Eco47I GGWCC 1 cut(s) 634
Eco53kI GAGCTC 1 cut(s) 143
EcoICRI GAGCTC 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 634
EcoRII CCWGG 1 cut(s) 499
EcoRV GATATC 1 cut(s) 246
EcoT14I CCWWGG 1 cut(s) 135
EcoT38I GRGCYC 1 cut(s) 145
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 457
FaeI CATG 3 cut(s) 206, 265, 368
FalI AAGNNNNNCTT 4 cut(s) 311, 343, 548, 580
FatI CATG 3 cut(s) 202, 261, 364
FbaI TGATCA 2 cut(s) 27, 199
Fnu4HI GCNGC 4 cut(s) 6, 294, 415, 471
FokI GGATG 2 cut(s) 75, 557
FriOI GRGCYC 1 cut(s) 145
Fsp4HI GCNGC 4 cut(s) 6, 294, 415, 471
FspBI CTAG 1 cut(s) 84
GlaI GCGC 2 cut(s) 76, 418
GluI GCNGC 4 cut(s) 6, 294, 415, 471
GsuI CTGGAG 1 cut(s) 439
HaeIII GGCC 3 cut(s) 5, 258, 296
HapII CCGG 1 cut(s) 42
HhaI GCGC 2 cut(s) 77, 419
Hin1II CATG 3 cut(s) 206, 265, 368
Hin6I GCGC 2 cut(s) 75, 417
HinP1I GCGC 2 cut(s) 75, 417
HinfI GANTC 1 cut(s) 324
HpaII CCGG 1 cut(s) 42
HphI GGTGA 2 cut(s) 226, 253
Hpy166II GTNNAC 1 cut(s) 362
Hpy188I TCNGA 5 cut(s) 12, 35, 153, 337, 602
Hpy188III TCNNGA 6 cut(s) 25, 160, 203, 319, 716, 734
Hpy8I GTNNAC 1 cut(s) 362
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 3 cut(s) 211, 679, 715
HpyCH4III ACNGT 1 cut(s) 121
HpyCH4IV ACGT 1 cut(s) 450
HpyCH4V TGCA 5 cut(s) 265, 389, 395, 470, 554
HpyF10VI GCNNNNNNNGC 5 cut(s) 51, 140, 386, 401, 425
HpyF3I CTNAG 4 cut(s) 9, 429, 538, 749
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 3 cut(s) 206, 265, 368
HspAI GCGC 2 cut(s) 75, 417
Ksp22I TGATCA 2 cut(s) 27, 199
LmnI GCTCC 2 cut(s) 140, 503
Lsp1109I GCAGC 1 cut(s) 482
MaeI CTAG 1 cut(s) 84
MaeII ACGT 1 cut(s) 450
MaeIII GTNAC 1 cut(s) 172
MbiI CCGCTC 2 cut(s) 8, 605
MboII GAAGA 4 cut(s) 92, 319, 587, 709
MflI RGATCY 2 cut(s) 21, 190
MhlI GDGCHC 1 cut(s) 145
MluCI AATT 2 cut(s) 338, 702
MnlI CCTC 9 cut(s) 29, 343, 418, 424, 647, 653, 660, 730, 758
MseI TTAA 1 cut(s) 567
MspA1I CMGCKG 1 cut(s) 251
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 1 cut(s) 501
MvaI CCWGG 1 cut(s) 501
MvnI CGCG 1 cut(s) 417
MwoI GCNNNNNNNGC 5 cut(s) 51, 140, 386, 401, 425
NheI GCTAGC 1 cut(s) 83
NlaIII CATG 3 cut(s) 206, 265, 368
NlaIV GGNNCC 3 cut(s) 40, 505, 589
NmuCI GTSAC 1 cut(s) 172
NspI RCATGY 2 cut(s) 265, 368
PagI TCATGA 1 cut(s) 202
PfeI GAWTC 1 cut(s) 324
PflFI GACNNNGTC 1 cut(s) 527
PkrI GCNGC 4 cut(s) 7, 295, 416, 472
PpuMI RGGWCCY 1 cut(s) 634
PshAI GACNNNNGTC 1 cut(s) 119
PsiI TTATAA 1 cut(s) 781
Psp124BI GAGCTC 1 cut(s) 145
Psp5II RGGWCCY 1 cut(s) 634
Psp6I CCWGG 1 cut(s) 499
PspGI CCWGG 1 cut(s) 499
PspN4I GGNNCC 3 cut(s) 40, 505, 589
PspPI GGNCC 2 cut(s) 256, 634
PspPPI RGGWCCY 1 cut(s) 634
PstNI CAGNNNCTG 1 cut(s) 462
PsuI RGATCY 2 cut(s) 21, 190
PsyI GACNNNGTC 1 cut(s) 527
RsaI GTAC 1 cut(s) 363
RsaNI GTAC 1 cut(s) 362
SacI GAGCTC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 567
SatI GCNGC 4 cut(s) 6, 294, 415, 471
Sau96I GGNCC 2 cut(s) 256, 634
ScrFI CCNGG 1 cut(s) 501
SduI GDGCHC 1 cut(s) 145
SfcI CTRYAG 1 cut(s) 420
SinI GGWCC 1 cut(s) 634
SmlI CTYRAG 2 cut(s) 644, 716
SmoI CTYRAG 2 cut(s) 644, 716
Sse9I AATT 2 cut(s) 338, 702
SsiI CCGC 7 cut(s) 6, 251, 293, 368, 412, 415, 605
SspMI CTAG 1 cut(s) 84
SstI GAGCTC 1 cut(s) 145
StyD4I CCNGG 1 cut(s) 499
StyI CCWWGG 1 cut(s) 135
TaaI ACNGT 1 cut(s) 121
TaiI ACGT 1 cut(s) 453
TaqI TCGA 2 cut(s) 66, 234
TasI AATT 2 cut(s) 338, 702
TatI WGTACW 1 cut(s) 361
TauI GCSGC 3 cut(s) 8, 296, 417
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 567
Tru9I TTAA 1 cut(s) 567
TscAI CASTG 1 cut(s) 630
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 470
Tsp45I GTSAC 1 cut(s) 172
TspDTI ATGAA 3 cut(s) 49, 413, 690
TspRI CASTG 1 cut(s) 630
Tth111I GACNNNGTC 1 cut(s) 527
VpaK11BI GGWCC 1 cut(s) 634
XapI RAATTY 1 cut(s) 338
XceI RCATGY 2 cut(s) 265, 368
XspI CTAG 1 cut(s) 84
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.