Rorug06G0303200

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0003310
Physical Location & Seq
Reverse (-)
45438823 .. 45439446
624 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0303200.1

Sequence Viewer

Length: 624 bp
ATGAATCTCTGCTCACACCCTCGACCACCTCCACACCCTCGACCAAACTCCACACCCTCATGGTGGAGTAAGAGAGAGCAAGTACCAACCTCTAACCTCGACCTCTACCGCTGGCTCCGCTTCCCCCTCGATTTCCTCCCCTTCCGCTCCCTCACCCCGTCGCCTCCTCCTCCGGCCTCCTCTACCTCTGGGCCGACTCCCCCGACTCCACCAAGTCCCTCGTCGTCTGCAACCCTCTCACCCGCCGCTTCCTCACCTTGCCTCAGCTCGGCTCCGCCTGGTCAAGGCACGGCTCCGTCCTCGTTGACTCCGCCACCCGTGTCATGGTCCTCACCTAGCTCGCCGCGCTTTACTTCTCCGCCGGCGCCGGCAACCACTGGCTCAAATTATCTTCCAATCTTCCGTCCAAGCCCCGCAGCCCGATCCTCGGCCGACTCGGTCTTCGCGCTCTGCGACGTCAGCTCGCCGTGGAGGAGCCAATGGAAGCTCTTCGCGTGCTCGACGATTCTGATACTGAGGCTGGATTTGGATAGCTGGGAGTGGGACGAGGCGGGCCGAATGCCGATGGATATGTTTATGATGAGAGAGAGAGAGAGCTCCTCTCTCTGTTTCAGATGTGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

207

Amino Acids

22.61

Weight (kDa)

8.65

Isoelectric Point (pI)

91.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 459
AccB1I GGYRCC 1 cut(s) 364
AccBSI CCGCTC 1 cut(s) 147
AccII CGCG 3 cut(s) 346, 446, 494
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 429
AcyI GRCGYC 2 cut(s) 365, 456
AfaI GTAC 1 cut(s) 84
AfiI CCNNNNNNNGG 5 cut(s) 63, 268, 284, 324, 427
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 6 cut(s) 267, 339, 462, 487, 534, 597
AluI AGCT 6 cut(s) 267, 339, 462, 487, 534, 597
Alw21I GWGCWC 2 cut(s) 500, 599
AlwI GGATC 1 cut(s) 417
AoxI GGCC 4 cut(s) 174, 191, 429, 553
ApeKI GCWGC 1 cut(s) 416
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
Asp700I GAANNNNTTC 1 cut(s) 488
AspLEI GCGC 3 cut(s) 348, 367, 448
AspS9I GGNCC 3 cut(s) 191, 327, 553
AsuHPI GGTGA 4 cut(s) 145, 231, 246, 324
AvaII GGWCC 1 cut(s) 327
BanI GGYRCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 599
BbsI GAAGAC 1 cut(s) 433
Bbv12I GWGCWC 2 cut(s) 500, 599
BbvCI CCTCAGC 1 cut(s) 263
BbvI GCAGC 1 cut(s) 428
BccI CCATC 1 cut(s) 559
BceAI ACGGC 2 cut(s) 306, 451
BciT130I CCWGG 1 cut(s) 279
BfaI CTAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 368
BisI GCNGC 3 cut(s) 246, 344, 417
BlsI GCNGC 3 cut(s) 247, 345, 418
Bme1390I CCNGG 1 cut(s) 279
Bme18I GGWCC 1 cut(s) 327
BmgT120I GGNCC 3 cut(s) 191, 327, 553
BmiI GGNNCC 5 cut(s) 116, 273, 294, 366, 476
BmrFI CCNGG 1 cut(s) 279
BpiI GAAGAC 1 cut(s) 433
BplI GAGNNNNNCTC 4 cut(s) 584, 616, 586, 618
Bpu10I CCTNAGC 1 cut(s) 263
BsaHI GRCGYC 2 cut(s) 365, 456
BsaJI CCNNGG 2 cut(s) 426, 467
Bsc4I CCNNNNNNNGG 5 cut(s) 63, 268, 284, 324, 427
Bse118I RCCGGY 2 cut(s) 361, 367
Bse1I ACTGG 1 cut(s) 382
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 2 cut(s) 426, 467
BseLI CCNNNNNNNGG 5 cut(s) 63, 268, 284, 324, 427
BseMII CTCAG 2 cut(s) 277, 506
BseNI ACTGG 1 cut(s) 382
BseRI GAGGAG 5 cut(s) 156, 159, 169, 487, 589
BseX3I CGGCCG 1 cut(s) 429
BseXI GCAGC 1 cut(s) 428
BseYI CCCAGC 1 cut(s) 534
Bsh1236I CGCG 3 cut(s) 346, 446, 494
Bsh1285I CGRYCG 1 cut(s) 432
BshFI GGCC 4 cut(s) 176, 193, 431, 555
BshNI GGYRCC 1 cut(s) 364
BsiEI CGRYCG 1 cut(s) 432
BsiHKAI GWGCWC 2 cut(s) 500, 599
BsiSI CCGG 3 cut(s) 173, 362, 368
BslFI GGGAC 2 cut(s) 201, 557
BslI CCNNNNNNNGG 5 cut(s) 63, 268, 284, 324, 427
BsmFI GGGAC 2 cut(s) 201, 557
BsmI GAATGC 1 cut(s) 564
BsnI GGCC 4 cut(s) 176, 193, 431, 555
Bsp1286I GDGCHC 2 cut(s) 500, 599
Bsp143I GATC 1 cut(s) 422
BspANI GGCC 4 cut(s) 176, 193, 431, 555
BspCNI CTCAG 2 cut(s) 276, 507
BspFNI CGCG 3 cut(s) 346, 446, 494
BspLI GGNNCC 5 cut(s) 116, 273, 294, 366, 476
BspPI GGATC 1 cut(s) 417
BspQI GCTCTTC 1 cut(s) 494
BspT107I GGYRCC 1 cut(s) 364
BsrBI CCGCTC 1 cut(s) 147
BsrFI RCCGGY 2 cut(s) 361, 367
BsrI ACTGG 1 cut(s) 382
BssAI RCCGGY 2 cut(s) 361, 367
BssECI CCNNGG 2 cut(s) 426, 467
BssMI GATC 1 cut(s) 422
BssNI GRCGYC 2 cut(s) 365, 456
Bst2UI CCWGG 1 cut(s) 279
Bst6I CTCTTC 1 cut(s) 494
BstACI GRCGYC 2 cut(s) 365, 456
BstC8I GCNNGC 7 cut(s) 113, 341, 363, 369, 464, 496, 553
BstDEI CTNAG 2 cut(s) 263, 515
BstDSI CCRYGG 1 cut(s) 467
BstENI CCTNNNNNAGG 1 cut(s) 282
BstFNI CGCG 3 cut(s) 346, 446, 494
BstH2I RGCGCY 1 cut(s) 368
BstHHI GCGC 3 cut(s) 348, 367, 448
BstKTI GATC 1 cut(s) 425
BstMBI GATC 1 cut(s) 422
BstMCI CGRYCG 1 cut(s) 432
BstMWI GCNNNNNNNGC 3 cut(s) 117, 345, 459
BstNI CCWGG 1 cut(s) 279
BstSCI CCNGG 1 cut(s) 277
BstUI CGCG 3 cut(s) 346, 446, 494
BstV1I GCAGC 1 cut(s) 428
BstV2I GAAGAC 1 cut(s) 433
BstZI CGGCCG 1 cut(s) 429
BsuRI GGCC 4 cut(s) 176, 193, 431, 555
BtgI CCRYGG 1 cut(s) 467
BtsIMutI CAGTG 1 cut(s) 375
Cac8I GCNNGC 7 cut(s) 113, 341, 363, 369, 464, 496, 553
CfoI GCGC 3 cut(s) 348, 367, 448
Cfr10I RCCGGY 2 cut(s) 361, 367
Cfr13I GGNCC 3 cut(s) 191, 327, 553
Csp6I GTAC 1 cut(s) 83
CviAII CATG 2 cut(s) 60, 324
CviQI GTAC 1 cut(s) 83
DdeI CTNAG 2 cut(s) 263, 515
DinI GGCGCC 1 cut(s) 366
DpnI GATC 1 cut(s) 424
DpnII GATC 1 cut(s) 422
EaeI YGGCCR 1 cut(s) 429
EagI CGGCCG 1 cut(s) 429
Eam1104I CTCTTC 1 cut(s) 494
EarI CTCTTC 1 cut(s) 494
EciI GGCGGA 3 cut(s) 264, 300, 348
Ecl136II GAGCTC 1 cut(s) 597
EclXI CGGCCG 1 cut(s) 429
Eco24I GRGCYC 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 327
Eco52I CGGCCG 1 cut(s) 429
Eco53kI GAGCTC 1 cut(s) 597
EcoICRI GAGCTC 1 cut(s) 597
EcoNI CCTNNNNNAGG 1 cut(s) 282
EcoRII CCWGG 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 599
EgeI GGCGCC 1 cut(s) 366
EheI GGCGCC 1 cut(s) 366
FaeI CATG 2 cut(s) 63, 327
FaiI YATR 4 cut(s) 61, 325, 572, 578
FaqI GGGAC 2 cut(s) 201, 557
FatI CATG 2 cut(s) 59, 323
FauI CCCGC 3 cut(s) 250, 421, 544
Fnu4HI GCNGC 3 cut(s) 246, 344, 417
FriOI GRGCYC 1 cut(s) 599
Fsp4HI GCNGC 3 cut(s) 246, 344, 417
FspBI CTAG 1 cut(s) 336
GlaI GCGC 3 cut(s) 347, 366, 447
GluI GCNGC 3 cut(s) 246, 344, 417
GsaI CCCAGC 1 cut(s) 538
HaeII RGCGCY 1 cut(s) 368
HaeIII GGCC 4 cut(s) 176, 193, 431, 555
HapII CCGG 3 cut(s) 173, 362, 368
HhaI GCGC 3 cut(s) 348, 367, 448
Hin1I GRCGYC 2 cut(s) 365, 456
Hin1II CATG 2 cut(s) 63, 327
Hin6I GCGC 3 cut(s) 346, 365, 446
HinP1I GCGC 3 cut(s) 346, 365, 446
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HinfI GANTC 6 cut(s) 4, 196, 205, 307, 434, 505
HpaII CCGG 3 cut(s) 173, 362, 368
HphI GGTGA 4 cut(s) 145, 231, 246, 324
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 2 cut(s) 510, 614
Hpy8I GTNNAC 1 cut(s) 306
Hpy99I CGWCG 4 cut(s) 163, 226, 458, 505
HpyAV CCTTC 1 cut(s) 151
HpyCH4IV ACGT 1 cut(s) 456
HpyCH4V TGCA 1 cut(s) 230
HpyF10VI GCNNNNNNNGC 3 cut(s) 117, 345, 459
HpyF3I CTNAG 2 cut(s) 263, 515
HpySE526I ACGT 1 cut(s) 456
Hsp92I GRCGYC 2 cut(s) 365, 456
Hsp92II CATG 2 cut(s) 63, 327
HspAI GCGC 3 cut(s) 346, 365, 446
KasI GGCGCC 1 cut(s) 364
KroI GCCGGC 2 cut(s) 361, 367
KroNI GCCGGC 2 cut(s) 363, 369
Kzo9I GATC 1 cut(s) 422
LguI GCTCTTC 1 cut(s) 494
LmnI GCTCC 6 cut(s) 120, 152, 277, 298, 474, 602
Lsp1109I GCAGC 1 cut(s) 428
MaeI CTAG 1 cut(s) 336
MaeII ACGT 1 cut(s) 456
MalI GATC 1 cut(s) 424
MbiI CCGCTC 1 cut(s) 147
MboI GATC 1 cut(s) 422
MboII GAAGA 4 cut(s) 383, 391, 433, 481
MhlI GDGCHC 2 cut(s) 500, 599
MluCI AATT 1 cut(s) 385
Mly113I GGCGCC 1 cut(s) 365
MlyI GAGTC 4 cut(s) 190, 199, 301, 428
MreI CGCCGGCG 1 cut(s) 361
MroNI GCCGGC 2 cut(s) 361, 367
MroXI GAANNNNTTC 1 cut(s) 488
MslI CAYNNNNRTG 1 cut(s) 58
MspA1I CMGCKG 1 cut(s) 111
MspI CCGG 3 cut(s) 173, 362, 368
MspR9I CCNGG 1 cut(s) 279
Mva1269I GAATGC 1 cut(s) 564
MvaI CCWGG 1 cut(s) 279
MvnI CGCG 3 cut(s) 346, 446, 494
MwoI GCNNNNNNNGC 3 cut(s) 117, 345, 459
NaeI GCCGGC 2 cut(s) 363, 369
NarI GGCGCC 1 cut(s) 365
NdeII GATC 1 cut(s) 422
NgoMIV GCCGGC 2 cut(s) 361, 367
NlaIII CATG 2 cut(s) 63, 327
NlaIV GGNNCC 5 cut(s) 116, 273, 294, 366, 476
NmeAIII GCCGAG 2 cut(s) 248, 407
PciSI GCTCTTC 1 cut(s) 494
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 1 cut(s) 564
PdiI GCCGGC 2 cut(s) 363, 369
PdmI GAANNNNTTC 1 cut(s) 488
PfeI GAWTC 2 cut(s) 4, 505
PflFI GACNNNGTC 1 cut(s) 437
PkrI GCNGC 3 cut(s) 247, 345, 418
PleI GAGTC 4 cut(s) 190, 199, 301, 428
PluTI GGCGCC 1 cut(s) 368
PpsI GAGTC 4 cut(s) 190, 199, 301, 428
Psp124BI GAGCTC 1 cut(s) 599
Psp6I CCWGG 1 cut(s) 277
PspFI CCCAGC 1 cut(s) 534
PspGI CCWGG 1 cut(s) 277
PspN4I GGNNCC 5 cut(s) 116, 273, 294, 366, 476
PspPI GGNCC 3 cut(s) 191, 327, 553
PsyI GACNNNGTC 1 cut(s) 437
RsaI GTAC 1 cut(s) 84
RsaNI GTAC 1 cut(s) 83
RseI CAYNNNNRTG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 599
SapI GCTCTTC 1 cut(s) 494
SatI GCNGC 3 cut(s) 246, 344, 417
Sau3AI GATC 1 cut(s) 422
Sau96I GGNCC 3 cut(s) 191, 327, 553
SchI GAGTC 4 cut(s) 190, 199, 301, 428
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 2 cut(s) 500, 599
SfoI GGCGCC 1 cut(s) 366
SgrAI CRCCGGYG 1 cut(s) 361
SinI GGWCC 1 cut(s) 327
SmiMI CAYNNNNRTG 1 cut(s) 58
Sse9I AATT 1 cut(s) 385
SspDI GGCGCC 1 cut(s) 364
SspMI CTAG 1 cut(s) 336
SstI GAGCTC 1 cut(s) 599
StyD4I CCNGG 1 cut(s) 277
TaiI ACGT 1 cut(s) 459
TaqI TCGA 5 cut(s) 22, 40, 99, 129, 500
TaqII GACCGA 1 cut(s) 427
TasI AATT 1 cut(s) 385
TauI GCSGC 2 cut(s) 248, 346
TfiI GAWTC 2 cut(s) 4, 505
TscAI CASTG 1 cut(s) 382
TseI GCWGC 1 cut(s) 416
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 2 cut(s) 285, 392
TspRI CASTG 1 cut(s) 382
Tth111I GACNNNGTC 1 cut(s) 437
VpaK11BI GGWCC 1 cut(s) 327
XagI CCTNNNNNAGG 1 cut(s) 282
XcmI CCANNNNNNNNNTGG 1 cut(s) 321
XmnI GAANNNNTTC 1 cut(s) 488
XspI CTAG 1 cut(s) 336
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.