pycom08g02330

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0003310
Physical Location & Seq
Reverse (-)
1725773 .. 1727549
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g02330.1

Sequence Viewer

Length: 990 bp
ATGGCTAAGAAGAGCAAGGTTTTGATCATCGGAGGTACTGGTTACATCGGAAAGTTTATCGTTCAAGCGAGCGCAAAGGCCGGCCATCCGACCTTCGCTTTGATTAGAGAGACCACGGGCAACGACCCTGGTAAGGCGACGAAGGCCGGCCATCCAACCTTTGCTCTGCTTAGAAGCACGGTCAACGATCCTTCTAAGGCTAACCTCATTGAGAAGTTTAAGAATTTGGGTGTCACTTTGCTCTACGGAGATCTTTATGACCATGAGAGTTTGGTGAAGGCGATCAAACAGGTGGACGTTGTGATATCAACGGTGGGTTTCAAGCAATTTGCAGACCAAACCAAGATCATTGCAGCCATCAAAGAAGCTGGCAATGTCAAGAGATTTTTCCCGTCGGAGTTTGGAAACGATGTGGATCGAGTGCATGCGGTTGAGCCAGCCAAGTCTACGTTTGACGTCAAGGTCCAAATCCGGCGTGCTGTTGAGGCTGAGGGCATTCCCTACACTTATGTCAACAACAACTTCTATGCTGGCTACTTCCTGTCCTCATTGGCACAGCCAGGTTCCACATCTCCACCCAGAGACAAAGTCACCATCTTAGGGGATGGAAATCCAAAAGCTGTCTTTAACAAGGAAGATGACATTGCAACCTATACAATCAGGGCCGTTGATGATCCAAGAACATTGAACAAGATCCTCTACGTGAAACCTCCCCAGAACATCTACTCATTCAATGGGCTTGTTGCCCTGTGGGAGAAGAAGATTGGCAAAGTCCTTGAAAAAGTCTATGTCCCAGAGGACAAAGTTCTCAAGGATATTGAAGAGGCCCCAACTCCAATCAATGTGATATTAGCAATCAACCACTCAGTGTTTGTGAAGGGAGATCAGACCAACTTCGAAATCGAGCCGTCGTTTGGAGTCGAAGCTTATGAGCTCTACCCTGATGTGAAGTACACAACTGTGGACGAGTACCTCGATCGATTTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

330

Amino Acids

36.41

Weight (kDa)

7.74

Isoelectric Point (pI)

27.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 3 - 41 2.1e-10 NmrA-like family
Epimerase PF01370 7 - 106 2.9e-07 NAD dependent epimerase/dehydratase family
NmrA PF05368 46 - 325 6.3e-86 NmrA-like family
NAD_binding_10 PF13460 51 - 172 4.5e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 461
AatII GACGTC 1 cut(s) 459
AccI GTMKAC 1 cut(s) 446
AciI CCGC 1 cut(s) 428
AclWI GGATC 4 cut(s) 182, 423, 668, 688
AcoI YGGCCR 2 cut(s) 82, 148
AcsI RAATTY 1 cut(s) 223
AcyI GRCGYC 1 cut(s) 456
AdeI CACNNNGTG 1 cut(s) 868
AfaI GTAC 3 cut(s) 37, 953, 971
AfiI CCNNNNNNNGG 3 cut(s) 133, 600, 914
AgsI TTSAA 6 cut(s) 65, 322, 688, 733, 779, 821
AjnI CCWGG 2 cut(s) 127, 559
AleI CACNNNNGTG 1 cut(s) 959
AluBI AGCT 4 cut(s) 368, 620, 926, 934
AluI AGCT 4 cut(s) 368, 620, 926, 934
Alw21I GWGCWC 1 cut(s) 936
Alw26I GTCTC 2 cut(s) 104, 576
AlwI GGATC 4 cut(s) 182, 423, 668, 688
AoxI GGCC 6 cut(s) 78, 82, 144, 148, 663, 825
ApeKI GCWGC 1 cut(s) 353
ApoI RAATTY 1 cut(s) 223
ArsI GACNNNNNNTTYG 2 cut(s) 82, 114
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 3 cut(s) 463, 663, 826
AsuHPI GGTGA 2 cut(s) 286, 583
AsuII TTCGAA 1 cut(s) 897
AvaII GGWCC 1 cut(s) 463
BanII GRGCYC 1 cut(s) 936
Bbv12I GWGCWC 1 cut(s) 936
BbvCI CCTCAGC 1 cut(s) 489
BbvI GCAGC 1 cut(s) 365
BccI CCATC 5 cut(s) 93, 159, 365, 599, 602
BceAI ACGGC 2 cut(s) 650, 892
BciT130I CCWGG 2 cut(s) 129, 561
BclI TGATCA 1 cut(s) 24
BcoDI GTCTC 2 cut(s) 104, 576
BglII AGATCT 1 cut(s) 250
BisI GCNGC 1 cut(s) 354
BlsI GCNGC 1 cut(s) 355
Bme1390I CCNGG 2 cut(s) 129, 561
Bme18I GGWCC 1 cut(s) 463
BmgT120I GGNCC 3 cut(s) 463, 663, 826
BmiI GGNNCC 2 cut(s) 565, 828
BmrFI CCNGG 2 cut(s) 129, 561
Bpu10I CCTNAGC 1 cut(s) 489
Bpu14I TTCGAA 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 794
Bsa29I ATCGAT 1 cut(s) 979
BsaAI YACGTR 1 cut(s) 703
BsaBI GATNNNNATC 2 cut(s) 414, 609
BsaHI GRCGYC 1 cut(s) 456
BsaI GGTCTC 1 cut(s) 104
BsaJI CCNNGG 2 cut(s) 114, 127
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 600, 914
Bse118I RCCGGY 2 cut(s) 80, 146
Bse1I ACTGG 1 cut(s) 43
Bse3DI GCAATG 3 cut(s) 348, 379, 642
Bse8I GATNNNNATC 2 cut(s) 414, 609
BseBI CCWGG 2 cut(s) 129, 561
BseCI ATCGAT 1 cut(s) 979
BseDI CCNNGG 2 cut(s) 114, 127
BseGI GGATG 3 cut(s) 85, 151, 610
BseJI GATNNNNATC 2 cut(s) 414, 609
BseLI CCNNNNNNNGG 3 cut(s) 133, 600, 914
BseMI GCAATG 3 cut(s) 348, 379, 642
BseMII CTCAG 2 cut(s) 480, 879
BseNI ACTGG 1 cut(s) 43
BseXI GCAGC 1 cut(s) 365
Bsh1285I CGRYCG 1 cut(s) 979
BshFI GGCC 6 cut(s) 80, 84, 146, 150, 665, 827
BshVI ATCGAT 1 cut(s) 979
BsiEI CGRYCG 1 cut(s) 979
BsiHKAI GWGCWC 1 cut(s) 936
BsiSI CCGG 3 cut(s) 81, 147, 472
BslFI GGGAC 1 cut(s) 776
BslI CCNNNNNNNGG 3 cut(s) 133, 600, 914
BsmAI GTCTC 2 cut(s) 104, 576
BsmFI GGGAC 1 cut(s) 776
BsmI GAATGC 1 cut(s) 495
BsnI GGCC 6 cut(s) 80, 84, 146, 150, 665, 827
Bso31I GGTCTC 1 cut(s) 104
Bsp119I TTCGAA 1 cut(s) 897
Bsp1286I GDGCHC 1 cut(s) 936
BspACI CCGC 1 cut(s) 428
BspANI GGCC 6 cut(s) 80, 84, 146, 150, 665, 827
BspCNI CTCAG 2 cut(s) 481, 878
BspDI ATCGAT 1 cut(s) 979
BspLI GGNNCC 2 cut(s) 565, 828
BspPI GGATC 4 cut(s) 182, 423, 668, 688
BspQI GCTCTTC 1 cut(s) 5
BspT104I TTCGAA 1 cut(s) 897
BspTNI GGTCTC 1 cut(s) 104
BsrDI GCAATG 3 cut(s) 348, 379, 642
BsrFI RCCGGY 2 cut(s) 80, 146
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 2 cut(s) 80, 146
BssECI CCNNGG 2 cut(s) 114, 127
BssNI GRCGYC 1 cut(s) 456
Bst2UI CCWGG 2 cut(s) 129, 561
Bst4CI ACNGT 3 cut(s) 181, 313, 961
Bst6I CTCTTC 2 cut(s) 5, 816
BstACI GRCGYC 1 cut(s) 456
BstBAI YACGTR 1 cut(s) 703
BstBI TTCGAA 1 cut(s) 897
BstC8I GCNNGC 8 cut(s) 70, 82, 148, 370, 426, 438, 477, 532
BstDEI CTNAG 6 cut(s) 6, 170, 195, 489, 598, 865
BstDSI CCRYGG 1 cut(s) 114
BstF5I GGATG 3 cut(s) 85, 151, 610
BstHHI GCGC 1 cut(s) 74
BstMAI GTCTC 2 cut(s) 104, 576
BstMCI CGRYCG 1 cut(s) 979
BstMWI GCNNNNNNNGC 2 cut(s) 143, 485
BstNI CCWGG 2 cut(s) 129, 561
BstNSI RCATGY 1 cut(s) 428
BstSCI CCNGG 2 cut(s) 127, 559
BstV1I GCAGC 1 cut(s) 365
BstX2I RGATCY 2 cut(s) 250, 693
BstYI RGATCY 2 cut(s) 250, 693
Bsu15I ATCGAT 1 cut(s) 979
BsuRI GGCC 6 cut(s) 80, 84, 146, 150, 665, 827
BsuTUI ATCGAT 1 cut(s) 979
BtgI CCRYGG 1 cut(s) 114
BtsCI GGATG 3 cut(s) 85, 151, 610
BtsIMutI CAGTG 1 cut(s) 873
Cac8I GCNNGC 8 cut(s) 70, 82, 148, 370, 426, 438, 477, 532
CfoI GCGC 1 cut(s) 74
Cfr10I RCCGGY 2 cut(s) 80, 146
Cfr13I GGNCC 3 cut(s) 463, 663, 826
ClaI ATCGAT 1 cut(s) 979
Csp6I GTAC 3 cut(s) 36, 952, 970
CviAII CATG 2 cut(s) 263, 425
CviQI GTAC 3 cut(s) 36, 952, 970
DdeI CTNAG 6 cut(s) 6, 170, 195, 489, 598, 865
DraIII CACNNNGTG 1 cut(s) 868
DrdI GACNNNNNNGTC 1 cut(s) 461
DseDI GACNNNNNNGTC 1 cut(s) 461
EaeI YGGCCR 2 cut(s) 82, 148
Eam1104I CTCTTC 2 cut(s) 5, 816
EarI CTCTTC 2 cut(s) 5, 816
Ecl136II GAGCTC 1 cut(s) 934
Eco24I GRGCYC 1 cut(s) 936
Eco31I GGTCTC 1 cut(s) 104
Eco32I GATATC 1 cut(s) 306
Eco47I GGWCC 1 cut(s) 463
Eco53kI GAGCTC 1 cut(s) 934
EcoICRI GAGCTC 1 cut(s) 934
EcoO109I RGGNCCY 1 cut(s) 826
EcoRII CCWGG 2 cut(s) 127, 559
EcoRV GATATC 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 936
FaeI CATG 2 cut(s) 266, 428
FaiI YATR 8 cut(s) 258, 264, 426, 510, 528, 654, 789, 930
FaqI GGGAC 1 cut(s) 776
FatI CATG 2 cut(s) 262, 424
FbaI TGATCA 1 cut(s) 24
FblI GTMKAC 1 cut(s) 446
Fnu4HI GCNGC 1 cut(s) 354
FokI GGATG 3 cut(s) 72, 138, 617
FriOI GRGCYC 1 cut(s) 936
FseI GGCCGGCC 2 cut(s) 84, 150
Fsp4HI GCNGC 1 cut(s) 354
GlaI GCGC 1 cut(s) 73
GluI GCNGC 1 cut(s) 354
HaeIII GGCC 6 cut(s) 80, 84, 146, 150, 665, 827
HapII CCGG 3 cut(s) 81, 147, 472
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 456
Hin1II CATG 2 cut(s) 266, 428
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HincII GTYRAC 2 cut(s) 184, 514
HindII GTYRAC 2 cut(s) 184, 514
HindIII AAGCTT 1 cut(s) 924
HinfI GANTC 1 cut(s) 918
HpaII CCGG 3 cut(s) 81, 147, 472
HphI GGTGA 2 cut(s) 286, 583
Hpy166II GTNNAC 6 cut(s) 184, 295, 447, 514, 954, 964
Hpy188I TCNGA 5 cut(s) 32, 50, 90, 397, 888
Hpy188III TCNNGA 1 cut(s) 379
Hpy8I GTNNAC 6 cut(s) 184, 295, 447, 514, 954, 964
Hpy99I CGWCG 3 cut(s) 142, 397, 913
HpyAV CCTTC 5 cut(s) 103, 136, 201, 271, 871
HpyCH4III ACNGT 3 cut(s) 181, 313, 961
HpyCH4IV ACGT 4 cut(s) 297, 449, 456, 702
HpyCH4V TGCA 4 cut(s) 332, 353, 424, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 143, 485
HpyF3I CTNAG 6 cut(s) 6, 170, 195, 489, 598, 865
HpySE526I ACGT 4 cut(s) 297, 449, 456, 702
Hsp92I GRCGYC 1 cut(s) 456
Hsp92II CATG 2 cut(s) 266, 428
HspAI GCGC 1 cut(s) 72
KroI GCCGGC 2 cut(s) 80, 146
KroNI GCCGGC 2 cut(s) 82, 148
Ksp22I TGATCA 1 cut(s) 24
LguI GCTCTTC 1 cut(s) 5
Lsp1109I GCAGC 1 cut(s) 365
MaeII ACGT 4 cut(s) 297, 449, 456, 702
MaeIII GTNAC 3 cut(s) 41, 232, 589
MboII GAAGA 5 cut(s) 22, 647, 769, 772, 833
MflI RGATCY 2 cut(s) 250, 693
MhlI GDGCHC 1 cut(s) 936
MluCI AATT 2 cut(s) 223, 326
MlyI GAGTC 1 cut(s) 927
MmeI TCCRAC 3 cut(s) 113, 179, 375
MroNI GCCGGC 2 cut(s) 80, 146
MseI TTAA 2 cut(s) 219, 627
MslI CAYNNNNRTG 1 cut(s) 959
MspI CCGG 3 cut(s) 81, 147, 472
MspR9I CCNGG 2 cut(s) 129, 561
Mva1269I GAATGC 1 cut(s) 495
MvaI CCWGG 2 cut(s) 129, 561
MwoI GCNNNNNNNGC 2 cut(s) 143, 485
NaeI GCCGGC 2 cut(s) 82, 148
NgoMIV GCCGGC 2 cut(s) 80, 146
NlaIII CATG 2 cut(s) 266, 428
NlaIV GGNNCC 2 cut(s) 565, 828
NmuCI GTSAC 2 cut(s) 232, 589
NspI RCATGY 1 cut(s) 428
NspV TTCGAA 1 cut(s) 897
OliI CACNNNNGTG 1 cut(s) 959
PaeI GCATGC 1 cut(s) 428
PciSI GCTCTTC 1 cut(s) 5
PcsI WCGNNNNNNNCGW 1 cut(s) 972
PctI GAATGC 1 cut(s) 495
PdiI GCCGGC 2 cut(s) 82, 148
PflFI GACNNNGTC 1 cut(s) 587
PkrI GCNGC 1 cut(s) 355
Ple19I CGATCG 1 cut(s) 979
PleI GAGTC 1 cut(s) 926
PpsI GAGTC 1 cut(s) 926
Ppu21I YACGTR 1 cut(s) 703
Psp124BI GAGCTC 1 cut(s) 936
Psp6I CCWGG 2 cut(s) 127, 559
PspGI CCWGG 2 cut(s) 127, 559
PspN4I GGNNCC 2 cut(s) 565, 828
PspPI GGNCC 3 cut(s) 463, 663, 826
PsuI RGATCY 2 cut(s) 250, 693
PsyI GACNNNGTC 1 cut(s) 587
PvuI CGATCG 1 cut(s) 979
RigI GGCCGGCC 2 cut(s) 84, 150
RsaI GTAC 3 cut(s) 37, 953, 971
RsaNI GTAC 3 cut(s) 36, 952, 970
RseI CAYNNNNRTG 1 cut(s) 959
SacI GAGCTC 1 cut(s) 936
SapI GCTCTTC 1 cut(s) 5
SaqAI TTAA 2 cut(s) 219, 627
SatI GCNGC 1 cut(s) 354
Sau96I GGNCC 3 cut(s) 463, 663, 826
SchI GAGTC 1 cut(s) 927
ScrFI CCNGG 2 cut(s) 129, 561
SduI GDGCHC 1 cut(s) 936
SfuI TTCGAA 1 cut(s) 897
SinI GGWCC 1 cut(s) 463
SmiMI CAYNNNNRTG 1 cut(s) 959
SmlI CTYRAG 1 cut(s) 809
SmoI CTYRAG 1 cut(s) 809
SphI GCATGC 1 cut(s) 428
Sse9I AATT 2 cut(s) 223, 326
SsiI CCGC 1 cut(s) 428
SstI GAGCTC 1 cut(s) 936
StyD4I CCNGG 2 cut(s) 127, 559
TaaI ACNGT 3 cut(s) 181, 313, 961
TaiI ACGT 4 cut(s) 300, 452, 459, 705
TaqI TCGA 6 cut(s) 418, 897, 903, 921, 975, 979
TasI AATT 2 cut(s) 223, 326
TatI WGTACW 1 cut(s) 951
Tru1I TTAA 2 cut(s) 219, 627
Tru9I TTAA 2 cut(s) 219, 627
TscAI CASTG 1 cut(s) 873
TseFI GTSAC 2 cut(s) 232, 589
TseI GCWGC 1 cut(s) 353
Tsp45I GTSAC 2 cut(s) 232, 589
TspGWI ACGGA 1 cut(s) 261
TspRI CASTG 1 cut(s) 873
Tth111I GACNNNGTC 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 463
XapI RAATTY 1 cut(s) 223
XceI RCATGY 1 cut(s) 428
XmiI GTMKAC 1 cut(s) 446
ZraI GACGTC 1 cut(s) 457
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.