RLG00000011261

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0003310
Physical Location & Seq
Reverse (-)
8056989 .. 8059031
2043 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000011261

Sequence Viewer

Length: 921 bp
ATGGCTGATCCTCAGAAAAGCAAGATTTTGGTAATCGGAGGCACCGGCTACATCGGCAAGTTCATCGTTGAAGCGAGCGCAAAGGCTAACCATCCTACCTTTGCTCTTGTTAGGGAGACCACTGTCAATGACCCAGCCAAGGAAAAACTCATTAAAAATTTCAAGAACCTGGGAGTCACTTTTCTCTATGGAGATCTTAATGACGAGCACGAGAGTTTGGTGAAGGCGATCAAACAGGTGGACGTGGTGATATCAACTGTGGGTGGCCTGCCGCTACCGGATCAAACCAAGCTCATTGCCGCCATTAAAGAAGCTGGTAATGTCCAGAGATTCCTCCCCTCGGAGTTCGGAACCGATGTGGATCGTGTGAATGATGCAGTTGAGCCGGCCAAATCTCTATATGTGACCAAAGCCCAAATCCGGCGTGCTATAGAGGCCGAGGGGATACCCTACACATATGTCTCCTGCAACTGCTTTGCAGGCTACTTCCTAGCCACATTTTTACAGCCAGGTGCCACTTCTCCACCCAGAGACAAAGTCATTATCTTAGGGGATGGAAATTCAAAGGCAATCTTTGTCAAGGAAGAGGACATCGGCACCTACACAATCCGAGCAGTTGATGACCCAAGAACACTGAACAAGGTCCTCCCAAAAACATTTACTCATTCAATGAACTGGTTGCCTTGTGGGAAAAGAAATTTGGCAAAGCCCTCGGGAAGGTCTATGTTCCAGAGGACAAACTCCTCAACGATATTACAGAGACCCCATTTCAATGTGATATTGGCGATCAACCACTCTGTGTTTGTGAAAGGAGATCAAACCAACTTCGAGATTGAACCATCGTTCGGAGTCGAAGCTTCCGAGCTATACCTGGATGTTGAATACACAACTGTGGAGGACTACCTCGACCAATTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

307

Amino Acids

33.82

Weight (kDa)

6.24

Isoelectric Point (pI)

29.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 302 1.7e-76 NmrA-like family
Epimerase PF01370 9 - 88 8.6e-08 NAD dependent epimerase/dehydratase family
Sacchrp_dh_NADP PF03435 9 - 89 2.4e-06 Saccharopine dehydrogenase NADP binding domain
NAD_binding_10 PF13460 13 - 155 1.2e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 41, 512, 596
AciI CCGC 2 cut(s) 272, 300
AclWI GGATC 3 cut(s) 2, 288, 369
AcoI YGGCCR 1 cut(s) 387
AcsI RAATTY 3 cut(s) 157, 559, 697
AdeI CACNNNGTG 1 cut(s) 799
AfiI CCNNNNNNNGG 5 cut(s) 139, 340, 420, 717, 845
AgsI TTSAA 7 cut(s) 71, 163, 564, 669, 772, 836, 881
AjiI CACGTC 1 cut(s) 244
AjnI CCWGG 3 cut(s) 168, 508, 870
AjuI GAANNNNNNNTTGG 2 cut(s) 683, 715
AleI CACNNNNGTG 1 cut(s) 890
AluBI AGCT 4 cut(s) 292, 314, 857, 865
AluI AGCT 4 cut(s) 292, 314, 857, 865
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 4 cut(s) 110, 466, 525, 754
AlwI GGATC 3 cut(s) 2, 288, 369
Ama87I CYCGRG 1 cut(s) 712
AoxI GGCC 3 cut(s) 265, 387, 435
ApoI RAATTY 3 cut(s) 157, 559, 697
AspLEI GCGC 1 cut(s) 80
AspS9I GGNCC 1 cut(s) 643
AsuHPI GGTGA 2 cut(s) 232, 259
AvaI CYCGRG 1 cut(s) 712
AvaII GGWCC 1 cut(s) 643
BanI GGYRCC 3 cut(s) 41, 512, 596
BauI CACGAG 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 3 cut(s) 99, 548, 847
BcgI CGANNNNNNTGC 4 cut(s) 46, 80, 693, 727
BciT130I CCWGG 3 cut(s) 170, 510, 872
BciVI GTATCC 1 cut(s) 438
BcoDI GTCTC 4 cut(s) 110, 466, 525, 754
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 429
BfuI GTATCC 1 cut(s) 438
BglII AGATCT 1 cut(s) 193
BisI GCNGC 2 cut(s) 272, 300
BlsI GCNGC 2 cut(s) 273, 301
Bme1390I CCNGG 3 cut(s) 170, 510, 872
Bme18I GGWCC 1 cut(s) 643
BmeT110I CYCGRG 1 cut(s) 712
BmgBI CACGTC 1 cut(s) 244
BmgT120I GGNCC 1 cut(s) 643
BmiI GGNNCC 4 cut(s) 43, 352, 514, 598
BmrFI CCNGG 3 cut(s) 170, 510, 872
BmsI GCATC 1 cut(s) 364
BoxI GACNNNNGTC 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 360
BsaI GGTCTC 2 cut(s) 110, 754
BsaJI CCNNGG 5 cut(s) 138, 169, 339, 438, 711
BsaWI WCCGGW 1 cut(s) 277
Bsc4I CCNNNNNNNGG 5 cut(s) 139, 340, 420, 717, 845
Bse118I RCCGGY 2 cut(s) 44, 385
Bse1I ACTGG 1 cut(s) 680
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 360
BseBI CCWGG 3 cut(s) 170, 510, 872
BseDI CCNNGG 5 cut(s) 138, 169, 339, 438, 711
BseGI GGATG 3 cut(s) 91, 559, 880
BseJI GATNNNNATC 1 cut(s) 360
BseLI CCNNNNNNNGG 5 cut(s) 139, 340, 420, 717, 845
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 26
BseNI ACTGG 1 cut(s) 680
BseRI GAGGAG 1 cut(s) 733
BseYI CCCAGC 1 cut(s) 133
BshFI GGCC 3 cut(s) 267, 389, 437
BshNI GGYRCC 3 cut(s) 41, 512, 596
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 712
BsiSI CCGG 4 cut(s) 45, 278, 386, 421
BslI CCNNNNNNNGG 5 cut(s) 139, 340, 420, 717, 845
BsmAI GTCTC 4 cut(s) 110, 466, 525, 754
BsnI GGCC 3 cut(s) 267, 389, 437
Bso31I GGTCTC 2 cut(s) 110, 754
BsoBI CYCGRG 1 cut(s) 712
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
BspACI CCGC 2 cut(s) 272, 300
BspANI GGCC 3 cut(s) 267, 389, 437
BspCNI CTCAG 1 cut(s) 25
BspLI GGNNCC 4 cut(s) 43, 352, 514, 598
BspPI GGATC 3 cut(s) 2, 288, 369
BspT107I GGYRCC 3 cut(s) 41, 512, 596
BspTNI GGTCTC 2 cut(s) 110, 754
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 2 cut(s) 44, 385
BsrI ACTGG 1 cut(s) 680
BssAI RCCGGY 2 cut(s) 44, 385
BssECI CCNNGG 5 cut(s) 138, 169, 339, 438, 711
BssMI GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
BssSI CACGAG 1 cut(s) 209
BssT1I CCWWGG 1 cut(s) 138
Bst2BI CACGAG 1 cut(s) 209
Bst2UI CCWGG 3 cut(s) 170, 510, 872
Bst4CI ACNGT 3 cut(s) 124, 259, 892
Bst6I CTCTTC 1 cut(s) 579
BstC8I GCNNGC 5 cut(s) 76, 269, 387, 426, 481
BstDEI CTNAG 2 cut(s) 12, 547
BstENI CCTNNNNNAGG 1 cut(s) 715
BstF5I GGATG 3 cut(s) 91, 559, 880
BstHHI GCGC 1 cut(s) 80
BstKTI GATC 7 cut(s) 10, 196, 231, 283, 364, 789, 817
BstMAI GTCTC 4 cut(s) 110, 466, 525, 754
BstMBI GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
BstMWI GCNNNNNNNGC 3 cut(s) 54, 434, 480
BstNI CCWGG 3 cut(s) 170, 510, 872
BstPAI GACNNNNGTC 1 cut(s) 122
BstSCI CCNGG 3 cut(s) 168, 508, 870
BstSFI CTRYAG 1 cut(s) 429
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
BsuI GTATCC 1 cut(s) 438
BsuRI GGCC 3 cut(s) 267, 389, 437
BtrI CACGTC 1 cut(s) 244
BtsCI GGATG 3 cut(s) 91, 559, 880
BtsIMutI CAGTG 2 cut(s) 120, 632
Cac8I GCNNGC 5 cut(s) 76, 269, 387, 426, 481
CfoI GCGC 1 cut(s) 80
Cfr10I RCCGGY 2 cut(s) 44, 385
Cfr13I GGNCC 1 cut(s) 643
DdeI CTNAG 2 cut(s) 12, 547
DpnI GATC 7 cut(s) 9, 195, 230, 282, 363, 788, 816
DpnII GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
DraIII CACNNNGTG 1 cut(s) 799
EaeI YGGCCR 1 cut(s) 387
Eam1104I CTCTTC 1 cut(s) 579
EarI CTCTTC 1 cut(s) 579
Eco130I CCWWGG 1 cut(s) 138
Eco31I GGTCTC 2 cut(s) 110, 754
Eco32I GATATC 1 cut(s) 252
Eco47I GGWCC 1 cut(s) 643
Eco88I CYCGRG 1 cut(s) 712
EcoNI CCTNNNNNAGG 1 cut(s) 715
EcoO109I RGGNCCY 1 cut(s) 643
EcoRII CCWGG 3 cut(s) 168, 508, 870
EcoRV GATATC 1 cut(s) 252
EcoT14I CCWWGG 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 138
FaiI YATR 8 cut(s) 189, 400, 402, 431, 457, 459, 725, 868
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FauNDI CATATG 1 cut(s) 457
Fnu4HI GCNGC 2 cut(s) 272, 300
FokI GGATG 3 cut(s) 78, 566, 887
Fsp4HI GCNGC 2 cut(s) 272, 300
FspBI CTAG 1 cut(s) 491
GlaI GCGC 1 cut(s) 79
GluI GCNGC 2 cut(s) 272, 300
GsaI CCCAGC 1 cut(s) 137
HaeIII GGCC 3 cut(s) 267, 389, 437
HapII CCGG 4 cut(s) 45, 278, 386, 421
HhaI GCGC 1 cut(s) 80
Hin6I GCGC 1 cut(s) 78
HinP1I GCGC 1 cut(s) 78
HindIII AAGCTT 1 cut(s) 855
HinfI GANTC 3 cut(s) 174, 330, 849
HpaII CCGG 4 cut(s) 45, 278, 386, 421
HphI GGTGA 2 cut(s) 232, 259
Hpy166II GTNNAC 1 cut(s) 241
Hpy188I TCNGA 7 cut(s) 15, 38, 343, 350, 611, 848, 862
Hpy188III TCNNGA 5 cut(s) 163, 325, 714, 730, 829
Hpy8I GTNNAC 1 cut(s) 241
HpyAV CCTTC 2 cut(s) 217, 711
HpyCH4III ACNGT 3 cut(s) 124, 259, 892
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 3 cut(s) 377, 468, 479
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 434, 480
HpyF3I CTNAG 2 cut(s) 12, 547
HpySE526I ACGT 1 cut(s) 243
HspAI GCGC 1 cut(s) 78
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Kzo9I GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
LweI GCATC 1 cut(s) 364
MaeI CTAG 1 cut(s) 491
MaeII ACGT 1 cut(s) 243
MaeIII GTNAC 2 cut(s) 175, 403
MalI GATC 7 cut(s) 9, 195, 230, 282, 363, 788, 816
MboI GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
MboII GAAGA 1 cut(s) 596
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 4 cut(s) 157, 559, 697, 911
MlyI GAGTC 2 cut(s) 183, 858
MroNI GCCGGC 1 cut(s) 385
MseI TTAA 4 cut(s) 153, 198, 306, 919
MslI CAYNNNNRTG 2 cut(s) 771, 890
MspI CCGG 4 cut(s) 45, 278, 386, 421
MspR9I CCNGG 3 cut(s) 170, 510, 872
MvaI CCWGG 3 cut(s) 170, 510, 872
MwoI GCNNNNNNNGC 3 cut(s) 54, 434, 480
NaeI GCCGGC 1 cut(s) 387
NdeI CATATG 1 cut(s) 457
NdeII GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
NgoMIV GCCGGC 1 cut(s) 385
NlaIV GGNNCC 4 cut(s) 43, 352, 514, 598
NmeAIII GCCGAG 1 cut(s) 463
NmuCI GTSAC 2 cut(s) 175, 403
OliI CACNNNNGTG 1 cut(s) 890
PcsI WCGNNNNNNNCGW 1 cut(s) 858
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 1 cut(s) 330
PflFI GACNNNGTC 1 cut(s) 536
PkrI GCNGC 2 cut(s) 273, 301
PleI GAGTC 2 cut(s) 182, 857
PpsI GAGTC 2 cut(s) 182, 857
PpuMI RGGWCCY 1 cut(s) 643
PshAI GACNNNNGTC 1 cut(s) 122
Psp5II RGGWCCY 1 cut(s) 643
Psp6I CCWGG 3 cut(s) 168, 508, 870
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 3 cut(s) 168, 508, 870
PspN4I GGNNCC 4 cut(s) 43, 352, 514, 598
PspPI GGNCC 1 cut(s) 643
PspPPI RGGWCCY 1 cut(s) 643
PsuI RGATCY 1 cut(s) 193
PsyI GACNNNGTC 1 cut(s) 536
RseI CAYNNNNRTG 2 cut(s) 771, 890
SaqAI TTAA 4 cut(s) 153, 198, 306, 919
SatI GCNGC 2 cut(s) 272, 300
Sau3AI GATC 7 cut(s) 7, 193, 228, 280, 361, 786, 814
Sau96I GGNCC 1 cut(s) 643
SchI GAGTC 2 cut(s) 183, 858
ScrFI CCNGG 3 cut(s) 170, 510, 872
SduI GDGCHC 1 cut(s) 210
SfaNI GCATC 1 cut(s) 364
SfcI CTRYAG 1 cut(s) 429
SinI GGWCC 1 cut(s) 643
SmiMI CAYNNNNRTG 2 cut(s) 771, 890
Sse9I AATT 4 cut(s) 157, 559, 697, 911
SsiI CCGC 2 cut(s) 272, 300
SspMI CTAG 1 cut(s) 491
StyD4I CCNGG 3 cut(s) 168, 508, 870
StyI CCWWGG 1 cut(s) 138
TaaI ACNGT 3 cut(s) 124, 259, 892
TaiI ACGT 1 cut(s) 246
TaqI TCGA 3 cut(s) 828, 852, 906
TasI AATT 4 cut(s) 157, 559, 697, 911
TauI GCSGC 2 cut(s) 274, 302
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 4 cut(s) 153, 198, 306, 919
Tru9I TTAA 4 cut(s) 153, 198, 306, 919
TscAI CASTG 2 cut(s) 127, 639
TseFI GTSAC 2 cut(s) 175, 403
Tsp45I GTSAC 2 cut(s) 175, 403
TspDTI ATGAA 2 cut(s) 52, 686
TspRI CASTG 2 cut(s) 127, 639
Tth111I GACNNNGTC 1 cut(s) 536
VpaK11BI GGWCC 1 cut(s) 643
XagI CCTNNNNNAGG 1 cut(s) 715
XapI RAATTY 3 cut(s) 157, 559, 697
XspI CTAG 1 cut(s) 491
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.