FvH4_2g39610

isoflavone

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0003310
Physical Location & Seq
Reverse (-)
28408376 .. 28410512
2137 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g39610.t1

Sequence Viewer

Length: 948 bp
ATGGCTGATCTTCAGAAAAGCAAGATTCTGGTAATCGGAGGCACCGGCTCCATCGGCAAGTTCATAGTCGAAGCAAGCGCAAAGGCTAACCATCCCACTTTCGCTCTTGTTAGAGAGACCACTGTCAATGACCCTGCCAAGGCAAAACTCATTCAAAAGTTCAAGAACTTGGGAGTCACTTTGCTCCATGGAGATATCAACGACCACGAGAGCTTGGTGAAGGTGATCAAACAGGTGGACGTGGTGATATCAACAGTGGGTGGTCCGCCACTACAGGATCAAACCAAAATCATTTCCGCCATCAAGGAAGCTGGTAATATCAAGAGATTCTTCCCATCGGAGTTCGGAAGCGATGTGGATCGTGTTCGTGTACATGACATAGTTGAGCCGGCAAAATCGTTTTTTGATAGCAAAATCCAAATCCGGCGTGCTATAGAGGCCGAGGGGATACCCCACACATATGTCACCAGCAATTGCTTTGCAGGCTATTTTTTACCCAGATTGGCACAGCGCCAGCCAGATGTTACTTCTCCACCCAGAGACAAAGTCATCATCTTCGGGGATGGAAATGCAAAGGCAATCTTTGTCGACGAAAAGGACATTGGAACCTATACAATCCGAGCAGTTGATGACCCAAGAACACTGAACAAGGTTCTCCACCTCAGGCCTCCTCGAAACTTTTACTCCTTCAATGAACTCGTTGCCTTGTGGGAGAAGAAAATTGGCAAACCACTTGAGAAGGTCTATCATCCAGAACAAAAACTCCTCGACGATATTAAAGCATCCCCATTTCCAGAAAGTGTGATATTGGGAATCAACCACTCTGTGTTTGTGAAAGGATTTCTTACTAACTTTGAGATTGAGCCATCCATCGGAGTCGAAGCTTCCGAGCTATACCCAGATGTTGAATACACCACTGTGGAGGACTACCTCGACCAATTTGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006979 GO:0008150 GO:0010035 GO:0010038 GO:0016020 GO:0042221 GO:0044464 GO:0046686 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NmrA

Protein Analysis

316

Amino Acids

35.14

Weight (kDa)

6.27

Isoelectric Point (pI)

29.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 311 5.4e-94 NmrA-like family
Epimerase PF01370 9 - 89 4.1e-08 NAD dependent epimerase/dehydratase family
Sacchrp_dh_NADP PF03435 9 - 89 7.4e-07 Saccharopine dehydrogenase NADP binding domain
NAD_binding_10 PF13460 13 - 155 1.5e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccI GTMKAC 1 cut(s) 588
AciI CCGC 2 cut(s) 266, 297
AclWI GGATC 2 cut(s) 285, 366
AdeI CACNNNGTG 1 cut(s) 826
AfaI GTAC 1 cut(s) 372
AfiI CCNNNNNNNGG 2 cut(s) 139, 872
AgsI TTSAA 4 cut(s) 155, 163, 691, 908
AjiI CACGTC 1 cut(s) 241
AjuI GAANNNNNNNTTGG 2 cut(s) 585, 617
AleI CACNNNNGTG 1 cut(s) 917
AloI GAACNNNNNNTCC 2 cut(s) 747, 779
AluBI AGCT 4 cut(s) 213, 311, 884, 892
AluI AGCT 4 cut(s) 213, 311, 884, 892
Alw26I GTCTC 2 cut(s) 110, 534
AlwI GGATC 2 cut(s) 285, 366
AoxI GGCC 2 cut(s) 438, 665
AspLEI GCGC 2 cut(s) 80, 513
AspS9I GGNCC 1 cut(s) 263
AsuHPI GGTGA 4 cut(s) 229, 235, 256, 457
AvaII GGWCC 1 cut(s) 263
AxyI CCTNAGG 1 cut(s) 662
BanI GGYRCC 1 cut(s) 41
BauI CACGAG 1 cut(s) 206
BccI CCATC 7 cut(s) 59, 99, 308, 343, 557, 874, 878
BciVI GTATCC 1 cut(s) 441
BclI TGATCA 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 110, 534
BfmI CTRYAG 2 cut(s) 272, 432
BfoI RGCGCY 1 cut(s) 514
BfuI GTATCC 1 cut(s) 441
Bme18I GGWCC 1 cut(s) 263
BmgBI CACGTC 1 cut(s) 241
BmgT120I GGNCC 1 cut(s) 263
BmiI GGNNCC 3 cut(s) 43, 49, 607
BmsI GCATC 1 cut(s) 791
BoxI GACNNNNGTC 1 cut(s) 122
BpuEI CTTGAG 1 cut(s) 755
BsaBI GATNNNNATC 1 cut(s) 357
BsaI GGTCTC 1 cut(s) 110
BsaJI CCNNGG 3 cut(s) 138, 187, 441
BsaXI ACNNNNNCTCC 4 cut(s) 668, 698, 747, 777
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 872
Bse118I RCCGGY 2 cut(s) 44, 388
Bse21I CCTNAGG 1 cut(s) 662
Bse8I GATNNNNATC 1 cut(s) 357
BseDI CCNNGG 3 cut(s) 138, 187, 441
BseGI GGATG 5 cut(s) 91, 568, 748, 782, 866
BseJI GATNNNNATC 1 cut(s) 357
BseLI CCNNNNNNNGG 2 cut(s) 139, 872
BseMII CTCAG 1 cut(s) 676
BseRI GAGGAG 2 cut(s) 660, 755
BshFI GGCC 2 cut(s) 440, 667
BshNI GGYRCC 1 cut(s) 41
BsiSI CCGG 3 cut(s) 45, 389, 424
BslI CCNNNNNNNGG 2 cut(s) 139, 872
BsmAI GTCTC 2 cut(s) 110, 534
BsnI GGCC 2 cut(s) 440, 667
Bso31I GGTCTC 1 cut(s) 110
Bsp1407I TGTACA 1 cut(s) 370
Bsp143I GATC 4 cut(s) 7, 225, 277, 358
Bsp19I CCATGG 1 cut(s) 187
BspACI CCGC 2 cut(s) 266, 297
BspANI GGCC 2 cut(s) 440, 667
BspCNI CTCAG 1 cut(s) 675
BspLI GGNNCC 3 cut(s) 43, 49, 607
BspPI GGATC 2 cut(s) 285, 366
BspT107I GGYRCC 1 cut(s) 41
BspTNI GGTCTC 1 cut(s) 110
BsrFI RCCGGY 2 cut(s) 44, 388
BsrGI TGTACA 1 cut(s) 370
BssAI RCCGGY 2 cut(s) 44, 388
BssECI CCNNGG 3 cut(s) 138, 187, 441
BssMI GATC 4 cut(s) 7, 225, 277, 358
BssSI CACGAG 1 cut(s) 206
BssT1I CCWWGG 2 cut(s) 138, 187
Bst2BI CACGAG 1 cut(s) 206
Bst4CI ACNGT 3 cut(s) 124, 256, 919
BstAUI TGTACA 1 cut(s) 370
BstC8I GCNNGC 5 cut(s) 76, 390, 429, 484, 515
BstDEI CTNAG 1 cut(s) 662
BstDSI CCRYGG 1 cut(s) 187
BstF5I GGATG 5 cut(s) 91, 568, 748, 782, 866
BstH2I RGCGCY 1 cut(s) 514
BstHHI GCGC 2 cut(s) 80, 513
BstKTI GATC 4 cut(s) 10, 228, 280, 361
BstMAI GTCTC 2 cut(s) 110, 534
BstMBI GATC 4 cut(s) 7, 225, 277, 358
BstMWI GCNNNNNNNGC 3 cut(s) 54, 437, 483
BstPAI GACNNNNGTC 1 cut(s) 122
BstSFI CTRYAG 2 cut(s) 272, 432
Bsu36I CCTNAGG 1 cut(s) 662
BsuI GTATCC 1 cut(s) 441
BsuRI GGCC 2 cut(s) 440, 667
BtgI CCRYGG 1 cut(s) 187
BtgZI GCGATG 1 cut(s) 366
BtrI CACGTC 1 cut(s) 241
BtsCI GGATG 5 cut(s) 91, 568, 748, 782, 866
BtsIMutI CAGTG 4 cut(s) 120, 261, 641, 915
Cac8I GCNNGC 5 cut(s) 76, 390, 429, 484, 515
CfoI GCGC 2 cut(s) 80, 513
Cfr10I RCCGGY 2 cut(s) 44, 388
Cfr13I GGNCC 1 cut(s) 263
Csp6I GTAC 1 cut(s) 371
CviAII CATG 2 cut(s) 188, 374
CviQI GTAC 1 cut(s) 371
DdeI CTNAG 1 cut(s) 662
DpnI GATC 4 cut(s) 9, 227, 279, 360
DpnII GATC 4 cut(s) 7, 225, 277, 358
DraIII CACNNNGTG 1 cut(s) 826
EciI GGCGGA 2 cut(s) 255, 286
Eco130I CCWWGG 2 cut(s) 138, 187
Eco147I AGGCCT 1 cut(s) 667
Eco31I GGTCTC 1 cut(s) 110
Eco32I GATATC 2 cut(s) 196, 249
Eco47I GGWCC 1 cut(s) 263
Eco81I CCTNAGG 1 cut(s) 662
EcoRV GATATC 2 cut(s) 196, 249
EcoT14I CCWWGG 2 cut(s) 138, 187
ErhI CCWWGG 2 cut(s) 138, 187
FaeI CATG 2 cut(s) 191, 377
FaiI YATR 9 cut(s) 65, 189, 375, 380, 434, 460, 462, 612, 895
FalI AAGNNNNNCTT 6 cut(s) 314, 346, 566, 598, 828, 860
FatI CATG 2 cut(s) 187, 373
FauNDI CATATG 1 cut(s) 460
FbaI TGATCA 1 cut(s) 225
FblI GTMKAC 1 cut(s) 588
FokI GGATG 5 cut(s) 78, 575, 735, 769, 853
GlaI GCGC 2 cut(s) 79, 512
HaeII RGCGCY 1 cut(s) 514
HaeIII GGCC 2 cut(s) 440, 667
HapII CCGG 3 cut(s) 45, 389, 424
HhaI GCGC 2 cut(s) 80, 513
Hin1II CATG 2 cut(s) 191, 377
Hin6I GCGC 2 cut(s) 78, 511
HinP1I GCGC 2 cut(s) 78, 511
HincII GTYRAC 1 cut(s) 589
HindII GTYRAC 1 cut(s) 589
HindIII AAGCTT 1 cut(s) 882
HinfI GANTC 5 cut(s) 25, 174, 327, 813, 876
HpaII CCGG 3 cut(s) 45, 389, 424
HphI GGTGA 4 cut(s) 229, 235, 256, 457
Hpy166II GTNNAC 3 cut(s) 238, 371, 589
Hpy188I TCNGA 7 cut(s) 15, 38, 340, 347, 620, 875, 889
Hpy188III TCNNGA 4 cut(s) 163, 322, 752, 794
Hpy8I GTNNAC 3 cut(s) 238, 371, 589
Hpy99I CGWCG 2 cut(s) 593, 773
HpyAV CCTTC 3 cut(s) 214, 697, 733
HpyCH4III ACNGT 3 cut(s) 124, 256, 919
HpyCH4IV ACGT 1 cut(s) 240
HpyCH4V TGCA 2 cut(s) 482, 572
HpyF10VI GCNNNNNNNGC 3 cut(s) 54, 437, 483
HpyF3I CTNAG 1 cut(s) 662
HpySE526I ACGT 1 cut(s) 240
Hsp92II CATG 2 cut(s) 191, 377
HspAI GCGC 2 cut(s) 78, 511
KroI GCCGGC 1 cut(s) 388
KroNI GCCGGC 1 cut(s) 390
Ksp22I TGATCA 1 cut(s) 225
Kzo9I GATC 4 cut(s) 7, 225, 277, 358
LmnI GCTCC 2 cut(s) 53, 189
LweI GCATC 1 cut(s) 791
MaeII ACGT 1 cut(s) 240
MaeIII GTNAC 3 cut(s) 175, 463, 523
MalI GATC 4 cut(s) 9, 227, 279, 360
MboI GATC 4 cut(s) 7, 225, 277, 358
MboII GAAGA 3 cut(s) 322, 547, 727
MfeI CAATTG 1 cut(s) 472
MluCI AATT 3 cut(s) 472, 720, 938
MlyI GAGTC 2 cut(s) 183, 885
MnlI CCTC 9 cut(s) 32, 430, 436, 671, 678, 681, 776, 916, 941
MroNI GCCGGC 1 cut(s) 388
MseI TTAA 2 cut(s) 777, 946
MslI CAYNNNNRTG 2 cut(s) 459, 917
MspI CCGG 3 cut(s) 45, 389, 424
MunI CAATTG 1 cut(s) 472
MwoI GCNNNNNNNGC 3 cut(s) 54, 437, 483
NaeI GCCGGC 1 cut(s) 390
NcoI CCATGG 1 cut(s) 187
NdeI CATATG 1 cut(s) 460
NdeII GATC 4 cut(s) 7, 225, 277, 358
NgoMIV GCCGGC 1 cut(s) 388
NlaIII CATG 2 cut(s) 191, 377
NlaIV GGNNCC 3 cut(s) 43, 49, 607
NmeAIII GCCGAG 1 cut(s) 466
NmuCI GTSAC 2 cut(s) 175, 463
OliI CACNNNNGTG 1 cut(s) 917
PceI AGGCCT 1 cut(s) 667
PcsI WCGNNNNNNNCGW 1 cut(s) 885
PdiI GCCGGC 1 cut(s) 390
PfeI GAWTC 3 cut(s) 25, 327, 813
PflFI GACNNNGTC 1 cut(s) 545
PleI GAGTC 2 cut(s) 182, 884
PpsI GAGTC 2 cut(s) 182, 884
PshAI GACNNNNGTC 1 cut(s) 122
PspN4I GGNNCC 3 cut(s) 43, 49, 607
PspPI GGNCC 1 cut(s) 263
PsyI GACNNNGTC 1 cut(s) 545
RsaI GTAC 1 cut(s) 372
RsaNI GTAC 1 cut(s) 371
RseI CAYNNNNRTG 2 cut(s) 459, 917
SalI GTCGAC 1 cut(s) 587
SaqAI TTAA 2 cut(s) 777, 946
Sau3AI GATC 4 cut(s) 7, 225, 277, 358
Sau96I GGNCC 1 cut(s) 263
SchI GAGTC 2 cut(s) 183, 885
SfaNI GCATC 1 cut(s) 791
SfcI CTRYAG 2 cut(s) 272, 432
SinI GGWCC 1 cut(s) 263
SmiMI CAYNNNNRTG 2 cut(s) 459, 917
SmlI CTYRAG 1 cut(s) 734
SmoI CTYRAG 1 cut(s) 734
Sse9I AATT 3 cut(s) 472, 720, 938
SseBI AGGCCT 1 cut(s) 667
SsiI CCGC 2 cut(s) 266, 297
StuI AGGCCT 1 cut(s) 667
StyI CCWWGG 2 cut(s) 138, 187
TaaI ACNGT 3 cut(s) 124, 256, 919
TaiI ACGT 1 cut(s) 243
TaqI TCGA 6 cut(s) 69, 588, 673, 768, 879, 933
TasI AATT 3 cut(s) 472, 720, 938
TatI WGTACW 1 cut(s) 370
TfiI GAWTC 3 cut(s) 25, 327, 813
Tru1I TTAA 2 cut(s) 777, 946
Tru9I TTAA 2 cut(s) 777, 946
TscAI CASTG 4 cut(s) 127, 261, 648, 922
TseFI GTSAC 2 cut(s) 175, 463
Tsp45I GTSAC 2 cut(s) 175, 463
TspDTI ATGAA 2 cut(s) 52, 708
TspRI CASTG 4 cut(s) 127, 261, 648, 922
Tth111I GACNNNGTC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 263
XmiI GTMKAC 1 cut(s) 588
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.