AT2G04900

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002952
Physical Location & Seq
Forward (+)
1722481 .. 1723976
1496 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G04900.1

Sequence Viewer

Length: 387 bp
ATGGGAAATAGTGTAAGAAGCAATCTAAGAGACATCAGAGGACGACGATCGATGGATCCTCGGATGTGGCACAAAGTCGCCGCTATTTCCGGTATGGCTGCTCTTGGTTTGGGAACTTATGGTGCTCATGTCTTTAAACCAGAGAACCCTTCTTACAAACAGGTGTGGCAAACGGCTTCACTTTACCATTTGGTTCACACTGCTGCTCTTGTTTCTGCTCCTAGCACCAAATATCCCAACATTTTTGGTGGCTTGTTGACTGCTGGAATTGTAGCCTTTTCCGGCACGTGTTATATGGTAGCGCTGCGGGAGGACAGAAAGTTTTCGACATTGGCACCATTCGGAGGCTTTGCGTTCATTGCTGCATGGGCAACTTTACTTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

128

Amino Acids

13.96

Weight (kDa)

10.17

Isoelectric Point (pI)

39.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 37 - 116 3.1e-23 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 334
AciI CCGC 2 cut(s) 81, 307
AclWI GGATC 2 cut(s) 50, 63
AcvI CACGTG 1 cut(s) 288
AfeI AGCGCT 1 cut(s) 303
AfiI CCNNNNNNNGG 1 cut(s) 344
AflIII ACRYGT 1 cut(s) 287
Alw21I GWGCWC 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 24
AlwI GGATC 2 cut(s) 50, 63
Aor51HI AGCGCT 1 cut(s) 303
ApeKI GCWGC 4 cut(s) 98, 203, 304, 362
Asp700I GAANNNNTTC 1 cut(s) 322
AspLEI GCGC 1 cut(s) 304
BamHI GGATCC 1 cut(s) 55
BanI GGYRCC 1 cut(s) 334
BbrPI CACGTG 1 cut(s) 288
Bbv12I GWGCWC 1 cut(s) 127
BbvI GCAGC 4 cut(s) 85, 190, 291, 349
BccI CCATC 1 cut(s) 46
BceAI ACGGC 1 cut(s) 189
BcoDI GTCTC 1 cut(s) 24
BfaI CTAG 1 cut(s) 222
BfoI RGCGCY 1 cut(s) 305
BisI GCNGC 5 cut(s) 81, 99, 204, 305, 363
BlsI GCNGC 5 cut(s) 82, 100, 205, 306, 364
BmiI GGNNCC 2 cut(s) 57, 336
Bsa29I ATCGAT 1 cut(s) 50
BsaAI YACGTR 1 cut(s) 288
BsaJI CCNNGG 1 cut(s) 59
BsaWI WCCGGW 1 cut(s) 89
Bsc4I CCNNNNNNNGG 1 cut(s) 344
Bse3DI GCAATG 1 cut(s) 357
BseCI ATCGAT 1 cut(s) 50
BseDI CCNNGG 1 cut(s) 59
BseGI GGATG 1 cut(s) 69
BseLI CCNNNNNNNGG 1 cut(s) 344
BseMI GCAATG 1 cut(s) 357
BseXI GCAGC 4 cut(s) 85, 190, 291, 349
Bsh1285I CGRYCG 1 cut(s) 50
BshNI GGYRCC 1 cut(s) 334
BshVI ATCGAT 1 cut(s) 50
BsiEI CGRYCG 1 cut(s) 50
BsiHKAI GWGCWC 1 cut(s) 127
BsiSI CCGG 2 cut(s) 90, 282
BslI CCNNNNNNNGG 1 cut(s) 344
BsmAI GTCTC 1 cut(s) 24
Bsp1286I GDGCHC 1 cut(s) 127
Bsp143I GATC 2 cut(s) 47, 55
BspACI CCGC 2 cut(s) 81, 307
BspDI ATCGAT 1 cut(s) 50
BspLI GGNNCC 2 cut(s) 57, 336
BspPI GGATC 2 cut(s) 50, 63
BspT107I GGYRCC 1 cut(s) 334
BsrDI GCAATG 1 cut(s) 357
BssECI CCNNGG 1 cut(s) 59
BssMI GATC 2 cut(s) 47, 55
BstBAI YACGTR 1 cut(s) 288
BstDEI CTNAG 1 cut(s) 26
BstF5I GGATG 1 cut(s) 69
BstH2I RGCGCY 1 cut(s) 305
BstHHI GCGC 1 cut(s) 304
BstKTI GATC 2 cut(s) 50, 58
BstMAI GTCTC 1 cut(s) 24
BstMBI GATC 2 cut(s) 47, 55
BstMCI CGRYCG 1 cut(s) 50
BstMWI GCNNNNNNNGC 2 cut(s) 359, 368
BstV1I GCAGC 4 cut(s) 85, 190, 291, 349
BstX2I RGATCY 1 cut(s) 55
BstYI RGATCY 1 cut(s) 55
Bsu15I ATCGAT 1 cut(s) 50
BsuTUI ATCGAT 1 cut(s) 50
BtsCI GGATG 1 cut(s) 69
BtsI GCAGTG 1 cut(s) 198
BtsIMutI CAGTG 1 cut(s) 198
CfoI GCGC 1 cut(s) 304
ClaI ATCGAT 1 cut(s) 50
CviAII CATG 2 cut(s) 128, 366
CviJI RGCY 5 cut(s) 98, 176, 252, 275, 348
CviKI_1 RGCY 5 cut(s) 98, 176, 252, 275, 348
DdeI CTNAG 1 cut(s) 26
DpnI GATC 2 cut(s) 49, 57
DpnII GATC 2 cut(s) 47, 55
DraI TTTAAA 1 cut(s) 136
Eco47III AGCGCT 1 cut(s) 303
Eco72I CACGTG 1 cut(s) 288
FaeI CATG 2 cut(s) 131, 369
FaiI YATR 6 cut(s) 95, 120, 129, 294, 296, 367
FatI CATG 2 cut(s) 127, 365
FauI CCCGC 1 cut(s) 300
Fnu4HI GCNGC 5 cut(s) 81, 99, 204, 305, 363
FokI GGATG 1 cut(s) 76
Fsp4HI GCNGC 5 cut(s) 81, 99, 204, 305, 363
FspBI CTAG 1 cut(s) 222
GlaI GCGC 1 cut(s) 303
GluI GCNGC 5 cut(s) 81, 99, 204, 305, 363
HaeII RGCGCY 1 cut(s) 305
HapII CCGG 2 cut(s) 90, 282
HhaI GCGC 1 cut(s) 304
Hin1II CATG 2 cut(s) 131, 369
Hin6I GCGC 1 cut(s) 302
HinP1I GCGC 1 cut(s) 302
HincII GTYRAC 1 cut(s) 258
HindII GTYRAC 1 cut(s) 258
HpaII CCGG 2 cut(s) 90, 282
Hpy166II GTNNAC 2 cut(s) 196, 258
Hpy188I TCNGA 3 cut(s) 38, 63, 344
Hpy8I GTNNAC 2 cut(s) 196, 258
Hpy99I CGWCG 1 cut(s) 48
HpyAV CCTTC 1 cut(s) 159
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 1 cut(s) 365
HpyF10VI GCNNNNNNNGC 2 cut(s) 359, 368
HpyF3I CTNAG 1 cut(s) 26
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 2 cut(s) 131, 369
HspAI GCGC 1 cut(s) 302
Kzo9I GATC 2 cut(s) 47, 55
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 5 cut(s) 103, 146, 153, 249, 295
Lsp1109I GCAGC 4 cut(s) 85, 190, 291, 349
MaeI CTAG 1 cut(s) 222
MaeII ACGT 1 cut(s) 287
MalI GATC 2 cut(s) 49, 57
MboI GATC 2 cut(s) 47, 55
MflI RGATCY 1 cut(s) 55
MhlI GDGCHC 1 cut(s) 127
MluCI AATT 1 cut(s) 267
MnlI CCTC 4 cut(s) 32, 69, 304, 338
MroXI GAANNNNTTC 1 cut(s) 322
MseI TTAA 1 cut(s) 135
MspI CCGG 2 cut(s) 90, 282
MwoI GCNNNNNNNGC 2 cut(s) 359, 368
NdeII GATC 2 cut(s) 47, 55
NlaIII CATG 2 cut(s) 131, 369
NlaIV GGNNCC 2 cut(s) 57, 336
PdmI GAANNNNTTC 1 cut(s) 322
PkrI GCNGC 5 cut(s) 82, 100, 205, 306, 364
Ple19I CGATCG 1 cut(s) 50
PmaCI CACGTG 1 cut(s) 288
PmlI CACGTG 1 cut(s) 288
Ppu21I YACGTR 1 cut(s) 288
PspCI CACGTG 1 cut(s) 288
PspN4I GGNNCC 2 cut(s) 57, 336
PsrI GAACNNNNNNTAC 2 cut(s) 137, 169
PsuI RGATCY 1 cut(s) 55
PvuI CGATCG 1 cut(s) 50
SaqAI TTAA 1 cut(s) 135
SatI GCNGC 5 cut(s) 81, 99, 204, 305, 363
Sau3AI GATC 2 cut(s) 47, 55
SduI GDGCHC 1 cut(s) 127
SetI ASST 2 cut(s) 165, 290
Sse9I AATT 1 cut(s) 267
SsiI CCGC 2 cut(s) 81, 307
SspMI CTAG 1 cut(s) 222
TaiI ACGT 1 cut(s) 290
TaqI TCGA 2 cut(s) 50, 326
TasI AATT 1 cut(s) 267
TauI GCSGC 1 cut(s) 83
Tru1I TTAA 1 cut(s) 135
Tru9I TTAA 1 cut(s) 135
TscAI CASTG 1 cut(s) 205
TseI GCWGC 4 cut(s) 98, 203, 304, 362
TspDTI ATGAA 1 cut(s) 346
TspRI CASTG 1 cut(s) 205
XmnI GAANNNNTTC 1 cut(s) 322
XspI CTAG 1 cut(s) 222
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.