Rh4DG108900

nicotianamine

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0002952
Physical Location & Seq
Forward (+)
16748484 .. 16756408
7925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG108900.1

Sequence Viewer

Length: 402 bp
ATGTTGGAACCTTTTGTGATCTTTGTTTATCTGGATTCTTTGGTTTATTTAGTATATTTAGTGCGGAGGTCAGTTACTCTTCATCACGACAGTGATCGGAGAGGTTTTTATTTGGTGGTTTGTATCAAATCTCCGAAAGGAAACCAAGCAAGGGAAGAAACAGCAATGAATCCTATACTATGGCACAAAGTAGCTGCAGTCTCTGGAATGGCAGCTTTTGGGTTGGGCATATATGGTGCTGATGGCTTTAAACCCAAAAACCCAGCTTACAAGGAGCTCACCAACATGGAATCCCAAATTCCTGTTGAGCTTCTCATAGCTCACATTATGCAACTCCATGCAAGCATATCCAAGCTCAACTCCCTTCGCCCTTCAAAGCAAGTCAACTCCCTCTTCACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NAS

Protein Analysis

133

Amino Acids

14.96

Weight (kDa)

9.3

Isoelectric Point (pI)

43.67

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 64
AcsI RAATTY 1 cut(s) 297
AfiI CCNNNNNNNGG 1 cut(s) 151
AgsI TTSAA 1 cut(s) 375
AleI CACNNNNGTG 1 cut(s) 90
AluBI AGCT 7 cut(s) 194, 215, 266, 277, 310, 320, 355
AluI AGCT 7 cut(s) 194, 215, 266, 277, 310, 320, 355
Alw21I GWGCWC 1 cut(s) 279
Alw26I GTCTC 1 cut(s) 205
AlwNI CAGNNNCTG 1 cut(s) 203
ApeKI GCWGC 2 cut(s) 194, 212
ApoI RAATTY 1 cut(s) 297
AsuHPI GGTGA 1 cut(s) 271
BanII GRGCYC 1 cut(s) 279
Bbv12I GWGCWC 1 cut(s) 279
BbvI GCAGC 2 cut(s) 181, 224
BccI CCATC 1 cut(s) 236
BcoDI GTCTC 1 cut(s) 205
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 2 cut(s) 195, 213
BlsI GCNGC 2 cut(s) 196, 214
BmiI GGNNCC 1 cut(s) 9
BsaAI YACGTR 1 cut(s) 399
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse3DI GCAATG 1 cut(s) 171
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMI GCAATG 1 cut(s) 171
BseXI GCAGC 2 cut(s) 181, 224
BseYI CCCAGC 1 cut(s) 262
BsiHKAI GWGCWC 1 cut(s) 279
BslI CCNNNNNNNGG 1 cut(s) 151
BsmAI GTCTC 1 cut(s) 205
Bsp1286I GDGCHC 1 cut(s) 279
Bsp143I GATC 2 cut(s) 18, 94
BspACI CCGC 1 cut(s) 64
BspLI GGNNCC 1 cut(s) 9
BspMAI CTGCAG 1 cut(s) 199
BsrDI GCAATG 1 cut(s) 171
BssMI GATC 2 cut(s) 18, 94
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 2 cut(s) 84, 398
BstBAI YACGTR 1 cut(s) 399
BstC8I GCNNGC 1 cut(s) 343
BstKTI GATC 2 cut(s) 21, 97
BstMAI GTCTC 1 cut(s) 205
BstMBI GATC 2 cut(s) 18, 94
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 2 cut(s) 181, 224
BtsIMutI CAGTG 1 cut(s) 97
Cac8I GCNNGC 1 cut(s) 343
CaiI CAGNNNCTG 1 cut(s) 203
CviAII CATG 2 cut(s) 286, 338
CviJI RGCY 8 cut(s) 194, 215, 246, 266, 277, 310, 320, 355
CviKI_1 RGCY 8 cut(s) 194, 215, 246, 266, 277, 310, 320, 355
DpnI GATC 2 cut(s) 20, 96
DpnII GATC 2 cut(s) 18, 94
DraI TTTAAA 1 cut(s) 250
Eam1104I CTCTTC 2 cut(s) 84, 398
EarI CTCTTC 2 cut(s) 84, 398
Ecl136II GAGCTC 1 cut(s) 277
Eco24I GRGCYC 1 cut(s) 279
Eco53kI GAGCTC 1 cut(s) 277
EcoICRI GAGCTC 1 cut(s) 277
EcoT38I GRGCYC 1 cut(s) 279
FaeI CATG 2 cut(s) 289, 341
FatI CATG 2 cut(s) 285, 337
Fnu4HI GCNGC 2 cut(s) 195, 213
FriOI GRGCYC 1 cut(s) 279
Fsp4HI GCNGC 2 cut(s) 195, 213
GluI GCNGC 2 cut(s) 195, 213
GsaI CCCAGC 1 cut(s) 266
Hin1II CATG 2 cut(s) 289, 341
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HinfI GANTC 3 cut(s) 35, 169, 290
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 385
Hpy188I TCNGA 2 cut(s) 99, 135
Hpy188III TCNNGA 3 cut(s) 32, 86, 204
Hpy8I GTNNAC 1 cut(s) 385
HpyAV CCTTC 2 cut(s) 374, 381
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4IV ACGT 1 cut(s) 398
HpyCH4V TGCA 3 cut(s) 197, 331, 341
HpySE526I ACGT 1 cut(s) 398
Hsp92II CATG 2 cut(s) 289, 341
Kzo9I GATC 2 cut(s) 18, 94
LmnI GCTCC 1 cut(s) 274
LpnPI CCDG 4 cut(s) 17, 189, 276, 315
Lsp1109I GCAGC 2 cut(s) 181, 224
MaeII ACGT 1 cut(s) 398
MaeIII GTNAC 1 cut(s) 73
MalI GATC 2 cut(s) 20, 96
MboI GATC 2 cut(s) 18, 94
MboII GAAGA 3 cut(s) 71, 167, 385
MhlI GDGCHC 1 cut(s) 279
MluCI AATT 1 cut(s) 297
MnlI CCTC 3 cut(s) 60, 95, 401
MseI TTAA 1 cut(s) 249
MslI CAYNNNNRTG 2 cut(s) 90, 284
NdeII GATC 2 cut(s) 18, 94
NlaIII CATG 2 cut(s) 289, 341
NlaIV GGNNCC 1 cut(s) 9
OliI CACNNNNGTG 1 cut(s) 90
PfeI GAWTC 3 cut(s) 35, 169, 290
PkrI GCNGC 2 cut(s) 196, 214
Ppu21I YACGTR 1 cut(s) 399
Psp124BI GAGCTC 1 cut(s) 279
PspFI CCCAGC 1 cut(s) 262
PspN4I GGNNCC 1 cut(s) 9
PstI CTGCAG 1 cut(s) 199
PstNI CAGNNNCTG 1 cut(s) 203
RseI CAYNNNNRTG 2 cut(s) 90, 284
SacI GAGCTC 1 cut(s) 279
SaqAI TTAA 1 cut(s) 249
SatI GCNGC 2 cut(s) 195, 213
Sau3AI GATC 2 cut(s) 18, 94
SduI GDGCHC 1 cut(s) 279
SfcI CTRYAG 1 cut(s) 195
SmiMI CAYNNNNRTG 2 cut(s) 90, 284
Sse9I AATT 1 cut(s) 297
SsiI CCGC 1 cut(s) 64
SstI GAGCTC 1 cut(s) 279
TaaI ACNGT 1 cut(s) 92
TaiI ACGT 1 cut(s) 401
TasI AATT 1 cut(s) 297
TfiI GAWTC 3 cut(s) 35, 169, 290
Tru1I TTAA 1 cut(s) 249
Tru9I TTAA 1 cut(s) 249
TscAI CASTG 1 cut(s) 97
TseI GCWGC 2 cut(s) 194, 212
TspDTI ATGAA 2 cut(s) 71, 182
TspRI CASTG 1 cut(s) 97
XapI RAATTY 1 cut(s) 297
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.