MD09G1123700.v1.1

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0002952
Physical Location & Seq
Reverse (-)
9567146 .. 9569166
2021 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1123700.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGATCCTCAGCTGTGGCATAAAGTAGCCGCGCTCTCTGGAATGGCAGCACTGGGGTTGGGCACATATGGCGCCCATGGCTTCAAACCCCAAAACCCAACTTACAAAGAGGTTTGGCAGACGGCGTCTCTGTACCATTTGGTTCACACAGCAGCTCTCCTTGCTGCCCCCATCACGAAACGCCCCACCATTTTTGGAGGCCTGTTGACTACTGGAATACTCGCATTCTCCGGGACGTGTTATACTGTGGCATTCCTTGAGGATAGAAAGTACTCGACATTGGCTCCTTTCGGTGGCTTTGCTTTCATCGCTGCTTGGGGTAGCTTGCTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

112

Amino Acids

11.92

Weight (kDa)

9.21

Isoelectric Point (pI)

25.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 20 - 99 2.2e-23 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccII CGCG 1 cut(s) 32
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 2 cut(s) 72, 125
AfaI GTAC 2 cut(s) 134, 272
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 1 cut(s) 85
AjiI CACGTC 1 cut(s) 237
AluBI AGCT 3 cut(s) 13, 155, 324
AluI AGCT 3 cut(s) 13, 155, 324
Alw26I GTCTC 1 cut(s) 132
AlwI GGATC 1 cut(s) 12
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 4 cut(s) 47, 152, 164, 311
AspLEI GCGC 2 cut(s) 34, 74
AsuC2I CCSGG 1 cut(s) 232
BaeGI GKGCMC 1 cut(s) 65
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 71
BbvCI CCTCAGC 1 cut(s) 9
BbvI GCAGC 4 cut(s) 59, 151, 164, 298
BccI CCATC 1 cut(s) 179
BceAI ACGGC 1 cut(s) 138
BcnI CCSGG 1 cut(s) 232
BcoDI GTCTC 1 cut(s) 132
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 5 cut(s) 30, 48, 153, 165, 312
BlsI GCNGC 5 cut(s) 31, 49, 154, 166, 313
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 232
BmgBI CACGTC 1 cut(s) 237
BmiI GGNNCC 3 cut(s) 6, 73, 285
BmrFI CCNGG 1 cut(s) 232
BmrI ACTGGG 1 cut(s) 62
BmuI ACTGGG 1 cut(s) 62
Bpu10I CCTNAGC 1 cut(s) 9
BpuEI CTTGAG 1 cut(s) 278
BpuMI CCSGG 1 cut(s) 232
BsaHI GRCGYC 2 cut(s) 72, 125
BsaJI CCNNGG 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 293
Bse1I ACTGG 2 cut(s) 57, 217
BseDI CCNNGG 1 cut(s) 76
BseLI CCNNNNNNNGG 1 cut(s) 293
BseMII CTCAG 1 cut(s) 23
BseNI ACTGG 2 cut(s) 57, 217
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 4 cut(s) 59, 151, 164, 298
Bsh1236I CGCG 1 cut(s) 32
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 71
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 293
BsmAI GTCTC 1 cut(s) 132
BsmBI CGTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 2 cut(s) 224, 251
BsnI GGCC 1 cut(s) 201
Bsp1286I GDGCHC 1 cut(s) 65
Bsp143I GATC 1 cut(s) 4
Bsp19I CCATGG 1 cut(s) 76
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 201
BspCNI CTCAG 1 cut(s) 22
BspFNI CGCG 1 cut(s) 32
BspLI GGNNCC 3 cut(s) 6, 73, 285
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 71
BsrI ACTGG 2 cut(s) 57, 217
BssECI CCNNGG 1 cut(s) 76
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 2 cut(s) 72, 125
BssT1I CCWWGG 1 cut(s) 76
Bst4CI ACNGT 1 cut(s) 247
BstACI GRCGYC 2 cut(s) 72, 125
BstC8I GCNNGC 1 cut(s) 326
BstDEI CTNAG 1 cut(s) 9
BstDSI CCRYGG 1 cut(s) 76
BstFNI CGCG 1 cut(s) 32
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 2 cut(s) 34, 74
BstKTI GATC 1 cut(s) 7
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 4 cut(s) 69, 78, 161, 308
BstSCI CCNGG 1 cut(s) 230
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 1 cut(s) 32
BstV1I GCAGC 4 cut(s) 59, 151, 164, 298
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 201
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 292
BtrI CACGTC 1 cut(s) 237
BtsIMutI CAGTG 1 cut(s) 50
Cac8I GCNNGC 1 cut(s) 326
CfoI GCGC 2 cut(s) 34, 74
CseI GACGC 1 cut(s) 114
Csp6I GTAC 2 cut(s) 133, 271
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 1 cut(s) 77
CviJI RGCY 8 cut(s) 13, 29, 81, 155, 201, 284, 297, 324
CviKI_1 RGCY 8 cut(s) 13, 29, 81, 155, 201, 284, 297, 324
CviQI GTAC 2 cut(s) 133, 271
DdeI CTNAG 1 cut(s) 9
DinI GGCGCC 1 cut(s) 73
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 76
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 76
Esp3I CGTCTC 1 cut(s) 132
FaeI CATG 1 cut(s) 80
FaiI YATR 5 cut(s) 21, 67, 69, 78, 243
FaqI GGGAC 1 cut(s) 247
FatI CATG 1 cut(s) 76
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 5 cut(s) 30, 48, 153, 165, 312
Fsp4HI GCNGC 5 cut(s) 30, 48, 153, 165, 312
GlaI GCGC 2 cut(s) 33, 73
GluI GCNGC 5 cut(s) 30, 48, 153, 165, 312
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 201
HapII CCGG 1 cut(s) 231
HgaI GACGC 1 cut(s) 114
HhaI GCGC 2 cut(s) 34, 74
Hin1I GRCGYC 2 cut(s) 72, 125
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 2 cut(s) 32, 72
HinP1I GCGC 2 cut(s) 32, 72
HincII GTYRAC 1 cut(s) 207
HindII GTYRAC 1 cut(s) 207
HpaII CCGG 1 cut(s) 231
Hpy166II GTNNAC 2 cut(s) 145, 207
Hpy188III TCNNGA 2 cut(s) 39, 175
Hpy8I GTNNAC 2 cut(s) 145, 207
HpyCH4III ACNGT 1 cut(s) 247
HpyCH4IV ACGT 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 78, 161, 308
HpyF3I CTNAG 1 cut(s) 9
HpySE526I ACGT 1 cut(s) 236
Hsp92I GRCGYC 2 cut(s) 72, 125
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 2 cut(s) 32, 72
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 5 cut(s) 24, 38, 198, 215, 244
Lsp1109I GCAGC 4 cut(s) 59, 151, 164, 298
MaeII ACGT 1 cut(s) 236
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 1 cut(s) 65
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 4 cut(s) 18, 103, 191, 253
MspA1I CMGCKG 1 cut(s) 13
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 232
Mva1269I GAATGC 2 cut(s) 224, 251
MvnI CGCG 1 cut(s) 32
MwoI GCNNNNNNNGC 4 cut(s) 69, 78, 161, 308
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 232
NcoI CCATGG 1 cut(s) 76
NdeI CATATG 1 cut(s) 67
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 3 cut(s) 6, 73, 285
PceI AGGCCT 1 cut(s) 201
PctI GAATGC 2 cut(s) 224, 251
PflFI GACNNNGTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 5 cut(s) 31, 49, 154, 166, 313
PluTI GGCGCC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 6, 73, 285
PsuI RGATCY 1 cut(s) 4
PsyI GACNNNGTC 1 cut(s) 124
PvuII CAGCTG 1 cut(s) 13
RsaI GTAC 2 cut(s) 134, 272
RsaNI GTAC 2 cut(s) 133, 271
SatI GCNGC 5 cut(s) 30, 48, 153, 165, 312
Sau3AI GATC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 232
SduI GDGCHC 1 cut(s) 65
SetI ASST 5 cut(s) 15, 114, 157, 239, 326
SfoI GGCGCC 1 cut(s) 73
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 1 cut(s) 30
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 230
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 1 cut(s) 247
TaiI ACGT 1 cut(s) 239
TaqI TCGA 1 cut(s) 275
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 32
TscAI CASTG 1 cut(s) 57
TseI GCWGC 4 cut(s) 47, 152, 164, 311
TspDTI ATGAA 1 cut(s) 295
TspRI CASTG 1 cut(s) 57
Tth111I GACNNNGTC 1 cut(s) 124
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.