MD17G1114500.v1.1

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002952
Physical Location & Seq
Reverse (-)
9811693 .. 9813589
1897 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1114500.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGATCCACAACTGTGGCACAAAGTAGCCGCCCTCTCTGGAATGGCAGCTCTTGGGTTGGGTACATATGGCGCCCATGTCTTCAAACCCAAAAACCCAACTTACAAAGAGGTTTGGCACACAGCTTCTCTCTACCATTTGGTTCACACAGCAGCTCTTCTTGCTGCCCCTATCACCAAGAACCCCACCATTTTTGGAGGCCTTTTGACTGCTGGAATACTCGCATTCTCCGGGACGTGTTATACTGTGGCATTACTCGAGGATAGAAAGTACTCTACGTTGGCTCCTTTCGGTGGCTTTGCTTTCATCGCTGCTTGGGGCAGCTTGCTATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

112

Amino Acids

11.87

Weight (kDa)

9.17

Isoelectric Point (pI)

21.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 20 - 99 7.4e-23 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AciI CCGC 1 cut(s) 30
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 2 cut(s) 64, 272
AfiI CCNNNNNNNGG 1 cut(s) 293
AflIII ACRYGT 1 cut(s) 236
AgsI TTSAA 1 cut(s) 85
AjiI CACGTC 1 cut(s) 237
AleI CACNNNNGTG 1 cut(s) 13
AluBI AGCT 4 cut(s) 50, 125, 155, 324
AluI AGCT 4 cut(s) 50, 125, 155, 324
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 257
AoxI GGCC 1 cut(s) 199
ApeKI GCWGC 5 cut(s) 47, 152, 164, 311, 321
AspLEI GCGC 1 cut(s) 74
AsuC2I CCSGG 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 166
AvaI CYCGRG 1 cut(s) 257
BamHI GGATCC 1 cut(s) 4
BanI GGYRCC 1 cut(s) 71
BbsI GAAGAC 1 cut(s) 73
BbvI GCAGC 4 cut(s) 59, 151, 164, 298
BcnI CCSGG 1 cut(s) 232
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
BlsI GCNGC 6 cut(s) 31, 49, 154, 166, 313, 323
BmcAI AGTACT 1 cut(s) 272
Bme1390I CCNGG 1 cut(s) 232
BmeT110I CYCGRG 1 cut(s) 257
BmgBI CACGTC 1 cut(s) 237
BmiI GGNNCC 3 cut(s) 6, 73, 285
BmrFI CCNGG 1 cut(s) 232
BpiI GAAGAC 1 cut(s) 73
BpuMI CCSGG 1 cut(s) 232
BsaHI GRCGYC 1 cut(s) 72
BsaXI ACNNNNNCTCC 2 cut(s) 268, 298
Bsc4I CCNNNNNNNGG 1 cut(s) 293
BseLI CCNNNNNNNGG 1 cut(s) 293
BseXI GCAGC 4 cut(s) 59, 151, 164, 298
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 257
BsiSI CCGG 1 cut(s) 231
BslFI GGGAC 1 cut(s) 247
BslI CCNNNNNNNGG 1 cut(s) 293
BsmFI GGGAC 1 cut(s) 247
BsmI GAATGC 1 cut(s) 224
BsnI GGCC 1 cut(s) 201
BsoBI CYCGRG 1 cut(s) 257
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 1 cut(s) 30
BspANI GGCC 1 cut(s) 201
BspLI GGNNCC 3 cut(s) 6, 73, 285
BspPI GGATC 1 cut(s) 12
BspQI GCTCTTC 1 cut(s) 162
BspT107I GGYRCC 1 cut(s) 71
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 1 cut(s) 72
Bst4CI ACNGT 2 cut(s) 15, 247
Bst6I CTCTTC 1 cut(s) 162
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 326
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 2 cut(s) 161, 308
BstSCI CCNGG 1 cut(s) 230
BstV1I GCAGC 4 cut(s) 59, 151, 164, 298
BstV2I GAAGAC 1 cut(s) 73
BstX2I RGATCY 1 cut(s) 4
BstXI CCANNNNNNTGG 1 cut(s) 15
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 1 cut(s) 201
BtgZI GCGATG 1 cut(s) 292
BtrI CACGTC 1 cut(s) 237
Cac8I GCNNGC 1 cut(s) 326
CfoI GCGC 1 cut(s) 74
Csp6I GTAC 2 cut(s) 63, 271
CspCI CAANNNNNGTGG 2 cut(s) 175, 210
CviAII CATG 1 cut(s) 77
CviJI RGCY 8 cut(s) 29, 50, 125, 155, 201, 284, 297, 324
CviKI_1 RGCY 8 cut(s) 29, 50, 125, 155, 201, 284, 297, 324
CviQI GTAC 2 cut(s) 63, 271
DinI GGCGCC 1 cut(s) 73
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
Eco147I AGGCCT 1 cut(s) 201
Eco88I CYCGRG 1 cut(s) 257
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
FaeI CATG 1 cut(s) 80
FaiI YATR 4 cut(s) 67, 69, 78, 243
FaqI GGGAC 1 cut(s) 247
FatI CATG 1 cut(s) 76
FauNDI CATATG 1 cut(s) 67
Fnu4HI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
Fsp4HI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
GlaI GCGC 1 cut(s) 73
GluI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 201
HapII CCGG 1 cut(s) 231
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HpaII CCGG 1 cut(s) 231
HphI GGTGA 1 cut(s) 166
Hpy166II GTNNAC 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 39
Hpy8I GTNNAC 1 cut(s) 145
HpyCH4III ACNGT 2 cut(s) 15, 247
HpyCH4IV ACGT 2 cut(s) 236, 278
HpyF10VI GCNNNNNNNGC 2 cut(s) 161, 308
HpySE526I ACGT 2 cut(s) 236, 278
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
Kzo9I GATC 1 cut(s) 4
LguI GCTCTTC 1 cut(s) 162
LmnI GCTCC 1 cut(s) 289
LpnPI CCDG 3 cut(s) 24, 198, 244
Lsp1109I GCAGC 4 cut(s) 59, 151, 164, 298
MaeII ACGT 2 cut(s) 236, 278
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 73, 149
MflI RGATCY 1 cut(s) 4
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 4 cut(s) 44, 103, 191, 253
MseI TTAA 1 cut(s) 334
MslI CAYNNNNRTG 1 cut(s) 13
MspI CCGG 1 cut(s) 231
MspR9I CCNGG 1 cut(s) 232
Mva1269I GAATGC 1 cut(s) 224
MwoI GCNNNNNNNGC 2 cut(s) 161, 308
NarI GGCGCC 1 cut(s) 72
NciI CCSGG 1 cut(s) 232
NdeI CATATG 1 cut(s) 67
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 3 cut(s) 6, 73, 285
OliI CACNNNNGTG 1 cut(s) 13
PaeR7I CTCGAG 1 cut(s) 257
PceI AGGCCT 1 cut(s) 201
PciSI GCTCTTC 1 cut(s) 162
PctI GAATGC 1 cut(s) 224
PfoI TCCNGGA 1 cut(s) 230
PkrI GCNGC 6 cut(s) 31, 49, 154, 166, 313, 323
PluTI GGCGCC 1 cut(s) 75
PspN4I GGNNCC 3 cut(s) 6, 73, 285
PspXI VCTCGAGB 1 cut(s) 257
PsuI RGATCY 1 cut(s) 4
RsaI GTAC 2 cut(s) 64, 272
RsaNI GTAC 2 cut(s) 63, 271
RseI CAYNNNNRTG 1 cut(s) 13
SapI GCTCTTC 1 cut(s) 162
SaqAI TTAA 1 cut(s) 334
SatI GCNGC 6 cut(s) 30, 48, 153, 165, 312, 322
Sau3AI GATC 1 cut(s) 4
ScaI AGTACT 1 cut(s) 272
ScrFI CCNGG 1 cut(s) 232
SetI ASST 7 cut(s) 52, 114, 127, 157, 239, 281, 326
SfoI GGCGCC 1 cut(s) 73
Sfr274I CTCGAG 1 cut(s) 257
SlaI CTCGAG 1 cut(s) 257
SmiMI CAYNNNNRTG 1 cut(s) 13
SmlI CTYRAG 1 cut(s) 257
SmoI CTYRAG 1 cut(s) 257
SseBI AGGCCT 1 cut(s) 201
SsiI CCGC 1 cut(s) 30
SspDI GGCGCC 1 cut(s) 71
StuI AGGCCT 1 cut(s) 201
StyD4I CCNGG 1 cut(s) 230
TaaI ACNGT 2 cut(s) 15, 247
TaiI ACGT 2 cut(s) 239, 281
TaqI TCGA 1 cut(s) 258
TatI WGTACW 1 cut(s) 270
TauI GCSGC 1 cut(s) 32
Tru1I TTAA 1 cut(s) 334
Tru9I TTAA 1 cut(s) 334
TseI GCWGC 5 cut(s) 47, 152, 164, 311, 321
TspDTI ATGAA 1 cut(s) 295
XhoI CTCGAG 1 cut(s) 257
ZrmI AGTACT 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.