MD17G1249100.v1.1

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002952
Physical Location & Seq
Reverse (-)
29775544 .. 29775806
263 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1249100.v1.1.491

Sequence Viewer

Length: 159 bp
ATGGCAGCACTTGGGTTGGGCACATATGGCGCCCATGGCTTCAAACCCCAACACCCAACTTACAAAGAGGTATGGCAGACGGCGTCTCTGTACCATTTGGTTCACACAGCAGCTCTGCTTGCTGCCCCCATCACGAAACACCCCACCATTGTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

53

Amino Acids

5.6

Weight (kDa)

9.3

Isoelectric Point (pI)

4.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 6 - 45 3.2e-11 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 29
AcyI GRCGYC 2 cut(s) 30, 83
AfaI GTAC 1 cut(s) 92
AgsI TTSAA 1 cut(s) 43
AleI CACNNNNGTG 1 cut(s) 149
AluBI AGCT 1 cut(s) 113
AluI AGCT 1 cut(s) 113
Alw26I GTCTC 1 cut(s) 90
ApeKI GCWGC 3 cut(s) 5, 110, 122
AspLEI GCGC 1 cut(s) 32
BaeGI GKGCMC 1 cut(s) 23
BanI GGYRCC 1 cut(s) 29
BbvI GCAGC 3 cut(s) 17, 109, 122
BccI CCATC 1 cut(s) 137
BceAI ACGGC 1 cut(s) 96
BcoDI GTCTC 1 cut(s) 90
BfoI RGCGCY 1 cut(s) 33
BisI GCNGC 3 cut(s) 6, 111, 123
BlsI GCNGC 3 cut(s) 7, 112, 124
BmiI GGNNCC 1 cut(s) 31
BsaHI GRCGYC 2 cut(s) 30, 83
BsaJI CCNNGG 1 cut(s) 34
BseDI CCNNGG 1 cut(s) 34
BseSI GKGCMC 1 cut(s) 23
BseXI GCAGC 3 cut(s) 17, 109, 122
BshNI GGYRCC 1 cut(s) 29
BsmAI GTCTC 1 cut(s) 90
BsmBI CGTCTC 1 cut(s) 90
Bsp1286I GDGCHC 1 cut(s) 23
Bsp19I CCATGG 1 cut(s) 34
BspLI GGNNCC 1 cut(s) 31
BspT107I GGYRCC 1 cut(s) 29
BssECI CCNNGG 1 cut(s) 34
BssNI GRCGYC 2 cut(s) 30, 83
BssT1I CCWWGG 1 cut(s) 34
BstACI GRCGYC 2 cut(s) 30, 83
BstC8I GCNNGC 1 cut(s) 120
BstDSI CCRYGG 1 cut(s) 34
BstH2I RGCGCY 1 cut(s) 33
BstHHI GCGC 1 cut(s) 32
BstMAI GTCTC 1 cut(s) 90
BstMWI GCNNNNNNNGC 3 cut(s) 27, 36, 119
BstSLI GKGCMC 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 17, 109, 122
BtgI CCRYGG 1 cut(s) 34
Cac8I GCNNGC 1 cut(s) 120
CfoI GCGC 1 cut(s) 32
CseI GACGC 1 cut(s) 72
Csp6I GTAC 1 cut(s) 91
CviAII CATG 1 cut(s) 35
CviJI RGCY 2 cut(s) 39, 113
CviKI_1 RGCY 2 cut(s) 39, 113
CviQI GTAC 1 cut(s) 91
DinI GGCGCC 1 cut(s) 31
Eco130I CCWWGG 1 cut(s) 34
EcoT14I CCWWGG 1 cut(s) 34
EgeI GGCGCC 1 cut(s) 31
EheI GGCGCC 1 cut(s) 31
ErhI CCWWGG 1 cut(s) 34
Esp3I CGTCTC 1 cut(s) 90
FaeI CATG 1 cut(s) 38
FaiI YATR 4 cut(s) 25, 27, 36, 73
FatI CATG 1 cut(s) 34
FauNDI CATATG 1 cut(s) 25
Fnu4HI GCNGC 3 cut(s) 6, 111, 123
Fsp4HI GCNGC 3 cut(s) 6, 111, 123
GlaI GCGC 1 cut(s) 31
GluI GCNGC 3 cut(s) 6, 111, 123
HaeII RGCGCY 1 cut(s) 33
HgaI GACGC 1 cut(s) 72
HhaI GCGC 1 cut(s) 32
Hin1I GRCGYC 2 cut(s) 30, 83
Hin1II CATG 1 cut(s) 38
Hin6I GCGC 1 cut(s) 30
HinP1I GCGC 1 cut(s) 30
Hpy166II GTNNAC 1 cut(s) 103
Hpy188III TCNNGA 1 cut(s) 133
Hpy8I GTNNAC 1 cut(s) 103
HpyF10VI GCNNNNNNNGC 3 cut(s) 27, 36, 119
Hsp92I GRCGYC 2 cut(s) 30, 83
Hsp92II CATG 1 cut(s) 38
HspAI GCGC 1 cut(s) 30
KasI GGCGCC 1 cut(s) 29
Lsp1109I GCAGC 3 cut(s) 17, 109, 122
MhlI GDGCHC 1 cut(s) 23
Mly113I GGCGCC 1 cut(s) 30
MnlI CCTC 1 cut(s) 61
MseI TTAA 1 cut(s) 157
MslI CAYNNNNRTG 1 cut(s) 149
MwoI GCNNNNNNNGC 3 cut(s) 27, 36, 119
NarI GGCGCC 1 cut(s) 30
NcoI CCATGG 1 cut(s) 34
NdeI CATATG 1 cut(s) 25
NlaIII CATG 1 cut(s) 38
NlaIV GGNNCC 1 cut(s) 31
OliI CACNNNNGTG 1 cut(s) 149
PflFI GACNNNGTC 1 cut(s) 82
PkrI GCNGC 3 cut(s) 7, 112, 124
PluTI GGCGCC 1 cut(s) 33
PspN4I GGNNCC 1 cut(s) 31
PsyI GACNNNGTC 1 cut(s) 82
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 1 cut(s) 149
SaqAI TTAA 1 cut(s) 157
SatI GCNGC 3 cut(s) 6, 111, 123
SduI GDGCHC 1 cut(s) 23
SetI ASST 2 cut(s) 72, 115
SfoI GGCGCC 1 cut(s) 31
SgeI CNNG 4 cut(s) 23, 47, 131, 145
SmiMI CAYNNNNRTG 1 cut(s) 149
SspDI GGCGCC 1 cut(s) 29
StyI CCWWGG 1 cut(s) 34
Tru1I TTAA 1 cut(s) 157
Tru9I TTAA 1 cut(s) 157
TseI GCWGC 3 cut(s) 5, 110, 122
Tth111I GACNNNGTC 1 cut(s) 82
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.