RLG00000020914

Protein of unknown function (DUF423)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002952
Physical Location & Seq
Forward (+)
70094291 .. 70095742
1452 bp
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UTR
Exon/CDS
Intron
RLM00000020914

Sequence Viewer

Length: 318 bp
ATGAATCCTATACTATGGCACAAAGTAGCTGCAGTCTCTGGAATGGCAGCTCTTGGGTTGGGCACATACGGCGCCCATGGCTTTAAGCCCCAAAACCCAGTTTACAAAGAGGTATATTTTCTATCAGGCACAGTTCATACTTTGTTATGTTGGTGGATATTAGGTAGTCTACTGTTTGGAGGCCTTTTAACTACTGGTATACTCGCATTCTCGGGGACGTGTTATACTGTGGCATTACTCGAAGATAGAAAGTACAATAAATTGGCTCCTTTTGGTGGAGTTGCTTTCATCGCAGCCTGGGCTAGCTTACTATTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF423

Protein Analysis

106

Amino Acids

11.33

Weight (kDa)

9.03

Isoelectric Point (pI)

22.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF423 PF04241 20 - 93 2.7e-09 Protein of unknown function (DUF423)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 71
AccI GTMKAC 2 cut(s) 169, 199
AcyI GRCGYC 1 cut(s) 72
AfaI GTAC 1 cut(s) 254
AfiI CCNNNNNNNGG 1 cut(s) 275
AflIII ACRYGT 1 cut(s) 218
AjiI CACGTC 1 cut(s) 219
AjnI CCWGG 1 cut(s) 296
AluBI AGCT 3 cut(s) 29, 50, 306
AluI AGCT 3 cut(s) 29, 50, 306
Alw26I GTCTC 1 cut(s) 40
AlwNI CAGNNNCTG 1 cut(s) 38
Ama87I CYCGRG 1 cut(s) 211
AoxI GGCC 1 cut(s) 181
ApeKI GCWGC 3 cut(s) 29, 47, 293
AspLEI GCGC 1 cut(s) 74
AsuNHI GCTAGC 1 cut(s) 302
AvaI CYCGRG 1 cut(s) 211
BaeGI GKGCMC 1 cut(s) 65
BanI GGYRCC 1 cut(s) 71
BbvI GCAGC 3 cut(s) 16, 59, 305
BceAI ACGGC 1 cut(s) 85
BciT130I CCWGG 1 cut(s) 298
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 303
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 3 cut(s) 30, 48, 294
BlsI GCNGC 3 cut(s) 31, 49, 295
Bme1390I CCNGG 1 cut(s) 298
BmeT110I CYCGRG 1 cut(s) 211
BmgBI CACGTC 1 cut(s) 219
BmiI GGNNCC 2 cut(s) 73, 267
BmrFI CCNGG 1 cut(s) 298
BmrI ACTGGG 1 cut(s) 92
BmtI GCTAGC 1 cut(s) 306
BmuI ACTGGG 1 cut(s) 92
BsaHI GRCGYC 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 76, 297
Bsc4I CCNNNNNNNGG 1 cut(s) 275
Bse1I ACTGG 2 cut(s) 98, 199
BseBI CCWGG 1 cut(s) 298
BseDI CCNNGG 2 cut(s) 76, 297
BseLI CCNNNNNNNGG 1 cut(s) 275
BseNI ACTGG 2 cut(s) 98, 199
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 3 cut(s) 16, 59, 305
BshFI GGCC 1 cut(s) 183
BshNI GGYRCC 1 cut(s) 71
BsiHKCI CYCGRG 1 cut(s) 211
BslFI GGGAC 1 cut(s) 229
BslI CCNNNNNNNGG 1 cut(s) 275
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 229
BsmI GAATGC 1 cut(s) 206
BsnI GGCC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 211
Bsp1286I GDGCHC 1 cut(s) 65
Bsp19I CCATGG 1 cut(s) 76
BspANI GGCC 1 cut(s) 183
BspLI GGNNCC 2 cut(s) 73, 267
BspMAI CTGCAG 1 cut(s) 34
BspOI GCTAGC 1 cut(s) 306
BspT107I GGYRCC 1 cut(s) 71
BsrI ACTGG 2 cut(s) 98, 199
BssECI CCNNGG 2 cut(s) 76, 297
BssNAI GTATAC 1 cut(s) 200
BssNI GRCGYC 1 cut(s) 72
BssT1I CCWWGG 1 cut(s) 76
Bst1107I GTATAC 1 cut(s) 200
Bst2UI CCWGG 1 cut(s) 298
Bst4CI ACNGT 3 cut(s) 133, 174, 229
BstACI GRCGYC 1 cut(s) 72
BstC8I GCNNGC 1 cut(s) 304
BstDSI CCRYGG 1 cut(s) 76
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstMAI GTCTC 1 cut(s) 40
BstMWI GCNNNNNNNGC 4 cut(s) 69, 78, 290, 299
BstNI CCWGG 1 cut(s) 298
BstSCI CCNGG 1 cut(s) 296
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 65
BstV1I GCAGC 3 cut(s) 16, 59, 305
BstZ17I GTATAC 1 cut(s) 200
BsuRI GGCC 1 cut(s) 183
BtgI CCRYGG 1 cut(s) 76
BtgZI GCGATG 1 cut(s) 274
BtrI CACGTC 1 cut(s) 219
Cac8I GCNNGC 1 cut(s) 304
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 1 cut(s) 74
Csp6I GTAC 1 cut(s) 253
CviAII CATG 1 cut(s) 77
CviJI RGCY 9 cut(s) 29, 50, 81, 88, 183, 266, 296, 302, 306
CviKI_1 RGCY 9 cut(s) 29, 50, 81, 88, 183, 266, 296, 302, 306
CviQI GTAC 1 cut(s) 253
DinI GGCGCC 1 cut(s) 73
Eco130I CCWWGG 1 cut(s) 76
Eco147I AGGCCT 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 296
EcoT14I CCWWGG 1 cut(s) 76
EgeI GGCGCC 1 cut(s) 73
EheI GGCGCC 1 cut(s) 73
ErhI CCWWGG 1 cut(s) 76
FaeI CATG 1 cut(s) 80
FaiI YATR 9 cut(s) 11, 16, 67, 78, 115, 138, 148, 200, 225
FaqI GGGAC 1 cut(s) 229
FatI CATG 1 cut(s) 76
FblI GTMKAC 2 cut(s) 169, 199
Fnu4HI GCNGC 3 cut(s) 30, 48, 294
Fsp4HI GCNGC 3 cut(s) 30, 48, 294
FspBI CTAG 1 cut(s) 303
GlaI GCGC 1 cut(s) 73
GluI GCNGC 3 cut(s) 30, 48, 294
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 1 cut(s) 183
HhaI GCGC 1 cut(s) 74
Hin1I GRCGYC 1 cut(s) 72
Hin1II CATG 1 cut(s) 80
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 103, 170, 200
Hpy188III TCNNGA 1 cut(s) 39
Hpy8I GTNNAC 3 cut(s) 103, 170, 200
HpyCH4III ACNGT 3 cut(s) 133, 174, 229
HpyCH4IV ACGT 1 cut(s) 218
HpyCH4V TGCA 1 cut(s) 32
HpyF10VI GCNNNNNNNGC 4 cut(s) 69, 78, 290, 299
HpySE526I ACGT 1 cut(s) 218
Hsp92I GRCGYC 1 cut(s) 72
Hsp92II CATG 1 cut(s) 80
HspAI GCGC 1 cut(s) 72
KasI GGCGCC 1 cut(s) 71
LmnI GCTCC 1 cut(s) 271
LpnPI CCDG 6 cut(s) 24, 111, 111, 180, 283, 310
Lsp1109I GCAGC 3 cut(s) 16, 59, 305
MaeI CTAG 1 cut(s) 303
MaeII ACGT 1 cut(s) 218
MboII GAAGA 1 cut(s) 254
MhlI GDGCHC 1 cut(s) 65
MluCI AATT 1 cut(s) 260
Mly113I GGCGCC 1 cut(s) 72
MnlI CCTC 2 cut(s) 103, 173
MseI TTAA 2 cut(s) 84, 188
MspR9I CCNGG 1 cut(s) 298
Mva1269I GAATGC 1 cut(s) 206
MvaI CCWGG 1 cut(s) 298
MwoI GCNNNNNNNGC 4 cut(s) 69, 78, 290, 299
NarI GGCGCC 1 cut(s) 72
NcoI CCATGG 1 cut(s) 76
NheI GCTAGC 1 cut(s) 302
NlaIII CATG 1 cut(s) 80
NlaIV GGNNCC 2 cut(s) 73, 267
PceI AGGCCT 1 cut(s) 183
PctI GAATGC 1 cut(s) 206
PfeI GAWTC 1 cut(s) 4
PkrI GCNGC 3 cut(s) 31, 49, 295
PluTI GGCGCC 1 cut(s) 75
Psp6I CCWGG 1 cut(s) 296
PspGI CCWGG 1 cut(s) 296
PspN4I GGNNCC 2 cut(s) 73, 267
PstI CTGCAG 1 cut(s) 34
PstNI CAGNNNCTG 1 cut(s) 38
RsaI GTAC 1 cut(s) 254
RsaNI GTAC 1 cut(s) 253
SaqAI TTAA 2 cut(s) 84, 188
SatI GCNGC 3 cut(s) 30, 48, 294
ScrFI CCNGG 1 cut(s) 298
SduI GDGCHC 1 cut(s) 65
SetI ASST 6 cut(s) 31, 52, 114, 166, 221, 308
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 1 cut(s) 73
Sse9I AATT 1 cut(s) 260
SseBI AGGCCT 1 cut(s) 183
SspDI GGCGCC 1 cut(s) 71
SspMI CTAG 1 cut(s) 303
StuI AGGCCT 1 cut(s) 183
StyD4I CCNGG 1 cut(s) 296
StyI CCWWGG 1 cut(s) 76
TaaI ACNGT 3 cut(s) 133, 174, 229
TaiI ACGT 1 cut(s) 221
TaqI TCGA 1 cut(s) 240
TasI AATT 1 cut(s) 260
TatI WGTACW 1 cut(s) 252
TfiI GAWTC 1 cut(s) 4
Tru1I TTAA 2 cut(s) 84, 188
Tru9I TTAA 2 cut(s) 84, 188
TseI GCWGC 3 cut(s) 29, 47, 293
TspDTI ATGAA 3 cut(s) 17, 125, 277
XmiI GTMKAC 2 cut(s) 169, 199
XspI CTAG 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.