AT2G40435

BEST Arabidopsis thaliana protein match is

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0001433
Physical Location & Seq
Forward (+)
16886767 .. 16889243
2477 bp
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UTR
Exon/CDS
Intron
AT2G40435.2

Sequence Viewer

Length: 477 bp
ATGGTTTCAAGGGAACAGAAGAGAGGTTCTTTGCAAGAGAAGTTTCAATTGCTTCGTTCAATCACTAATTCACATGCTGAAAACGATACATCGATCATAATGGATGCATCAAAATATATCCAAAAGCTTAAGCAGAAAGTCGAAAGATTCAACCAAGACCCTACTGCAGAGCAATCTTCAAGCGAGCCCACGGATCCAAAAACACCAATGGTAACAGTGGAAACCCTAGACAAGGGTTTTATGATAAACGTGTTCTCAGGAAAGAATCAGCCAGGGATGCTTGTTTCGGTATTAGAAGCCTTCGAGGATATTGGGCTTAACGTTCTTGAAGCTAGGGCTTCATGTACCGACTCTTTTAGCTTGCATGCCATGGGACTCGAGAATGAAGATGGAGAAAATATGGATGCTGAAGCAGTGAAACAGGCAGTGACTGATGCAATCAGGAGCTGGGGTGAAATCAATGATCCACAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

17.57

Weight (kDa)

4.65

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 70 - 126 5.3e-17 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 321
AclWI GGATC 3 cut(s) 188, 201, 458
AcuI CTGAAG 1 cut(s) 429
AfaI GTAC 1 cut(s) 346
AfiI CCNNNNNNNGG 1 cut(s) 232
AflII CTTAAG 1 cut(s) 128
AflIII ACRYGT 1 cut(s) 249
AgsI TTSAA 6 cut(s) 9, 47, 60, 151, 180, 329
AjnI CCWGG 1 cut(s) 271
AluBI AGCT 4 cut(s) 127, 332, 360, 447
AluI AGCT 4 cut(s) 127, 332, 360, 447
AlwI GGATC 3 cut(s) 188, 201, 458
AlwNI CAGNNNCTG 2 cut(s) 431, 447
Ama87I CYCGRG 1 cut(s) 377
AsuHPI GGTGA 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 377
BamHI GGATCC 1 cut(s) 193
BanII GRGCYC 1 cut(s) 189
BccI CCATC 1 cut(s) 383
BciT130I CCWGG 1 cut(s) 273
BfaI CTAG 3 cut(s) 227, 333, 475
BfmI CTRYAG 1 cut(s) 165
BfrI CTTAAG 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 273
BmeT110I CYCGRG 1 cut(s) 377
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 1 cut(s) 273
BmsI GCATC 5 cut(s) 94, 116, 267, 394, 424
Bsa29I ATCGAT 1 cut(s) 92
BsaJI CCNNGG 3 cut(s) 189, 272, 369
BsaXI ACNNNNNCTCC 2 cut(s) 436, 466
Bsc4I CCNNNNNNNGG 1 cut(s) 232
BseBI CCWGG 1 cut(s) 273
BseCI ATCGAT 1 cut(s) 92
BseDI CCNNGG 3 cut(s) 189, 272, 369
BseGI GGATG 3 cut(s) 109, 282, 409
BseLI CCNNNNNNNGG 1 cut(s) 232
BseMII CTCAG 1 cut(s) 270
BseYI CCCAGC 1 cut(s) 447
BshVI ATCGAT 1 cut(s) 92
BsiHKCI CYCGRG 1 cut(s) 377
BslFI GGGAC 1 cut(s) 387
BslI CCNNNNNNNGG 1 cut(s) 232
BsmFI GGGAC 1 cut(s) 387
BsoBI CYCGRG 1 cut(s) 377
Bsp1286I GDGCHC 1 cut(s) 189
Bsp143I GATC 3 cut(s) 93, 193, 463
Bsp19I CCATGG 1 cut(s) 369
BspCNI CTCAG 1 cut(s) 269
BspDI ATCGAT 1 cut(s) 92
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 169
BspPI GGATC 3 cut(s) 188, 201, 458
BspTI CTTAAG 1 cut(s) 128
BssECI CCNNGG 3 cut(s) 189, 272, 369
BssMI GATC 3 cut(s) 93, 193, 463
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 273
Bst4CI ACNGT 1 cut(s) 217
Bst6I CTCTTC 1 cut(s) 14
BstAFI CTTAAG 1 cut(s) 128
BstC8I GCNNGC 3 cut(s) 185, 362, 366
BstDEI CTNAG 1 cut(s) 256
BstDSI CCRYGG 2 cut(s) 189, 369
BstENI CCTNNNNNAGG 1 cut(s) 230
BstF5I GGATG 3 cut(s) 109, 282, 409
BstKTI GATC 3 cut(s) 96, 196, 466
BstMBI GATC 3 cut(s) 93, 193, 463
BstMWI GCNNNNNNNGC 1 cut(s) 277
BstNI CCWGG 1 cut(s) 273
BstNSI RCATGY 2 cut(s) 77, 368
BstSCI CCNGG 1 cut(s) 271
BstSFI CTRYAG 1 cut(s) 165
BstX2I RGATCY 1 cut(s) 193
BstYI RGATCY 1 cut(s) 193
Bsu15I ATCGAT 1 cut(s) 92
BsuTUI ATCGAT 1 cut(s) 92
BtgI CCRYGG 2 cut(s) 189, 369
BtsCI GGATG 3 cut(s) 109, 282, 409
BtsI GCAGTG 2 cut(s) 420, 432
BtsIMutI CAGTG 3 cut(s) 222, 420, 432
Cac8I GCNNGC 3 cut(s) 185, 362, 366
CaiI CAGNNNCTG 2 cut(s) 431, 447
ClaI ATCGAT 1 cut(s) 92
Csp6I GTAC 1 cut(s) 345
CviAII CATG 4 cut(s) 74, 342, 365, 370
CviJI RGCY 9 cut(s) 127, 187, 271, 299, 316, 332, 338, 360, 447
CviKI_1 RGCY 9 cut(s) 127, 187, 271, 299, 316, 332, 338, 360, 447
CviQI GTAC 1 cut(s) 345
DdeI CTNAG 1 cut(s) 256
DpnI GATC 3 cut(s) 95, 195, 465
DpnII GATC 3 cut(s) 93, 193, 463
Eam1104I CTCTTC 1 cut(s) 14
EarI CTCTTC 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 189
Eco57I CTGAAG 1 cut(s) 429
Eco88I CYCGRG 1 cut(s) 377
EcoNI CCTNNNNNAGG 1 cut(s) 230
EcoRII CCWGG 1 cut(s) 271
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 189
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 4 cut(s) 77, 345, 368, 373
FaiI YATR 8 cut(s) 75, 98, 117, 242, 343, 366, 371, 401
FaqI GGGAC 1 cut(s) 387
FatI CATG 4 cut(s) 73, 341, 364, 369
FokI GGATG 3 cut(s) 116, 289, 416
FriOI GRGCYC 1 cut(s) 189
FspBI CTAG 3 cut(s) 227, 333, 475
GsaI CCCAGC 1 cut(s) 451
Hin1II CATG 4 cut(s) 77, 345, 368, 373
HindIII AAGCTT 1 cut(s) 125
HinfI GANTC 4 cut(s) 147, 265, 350, 375
HphI GGTGA 1 cut(s) 464
Hpy188III TCNNGA 4 cut(s) 258, 326, 379, 442
HpyAV CCTTC 1 cut(s) 310
HpyCH4III ACNGT 1 cut(s) 217
HpyCH4IV ACGT 2 cut(s) 249, 321
HpyCH4V TGCA 5 cut(s) 34, 107, 167, 364, 437
HpyF10VI GCNNNNNNNGC 1 cut(s) 277
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 2 cut(s) 249, 321
Hsp92II CATG 4 cut(s) 77, 345, 368, 373
Kzo9I GATC 3 cut(s) 93, 193, 463
LmnI GCTCC 1 cut(s) 444
LpnPI CCDG 6 cut(s) 243, 258, 285, 407, 427, 433
LweI GCATC 5 cut(s) 94, 116, 267, 394, 424
MaeI CTAG 3 cut(s) 227, 333, 475
MaeII ACGT 2 cut(s) 249, 321
MaeIII GTNAC 2 cut(s) 211, 427
MalI GATC 3 cut(s) 95, 195, 465
MboI GATC 3 cut(s) 93, 193, 463
MboII GAAGA 3 cut(s) 31, 168, 398
MfeI CAATTG 1 cut(s) 47
MflI RGATCY 1 cut(s) 193
MhlI GDGCHC 1 cut(s) 189
MluCI AATT 2 cut(s) 47, 67
MlyI GAGTC 2 cut(s) 344, 369
MnlI CCTC 2 cut(s) 17, 298
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 2 cut(s) 129, 318
MspCI CTTAAG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 273
MunI CAATTG 1 cut(s) 47
MvaI CCWGG 1 cut(s) 273
MwoI GCNNNNNNNGC 1 cut(s) 277
NcoI CCATGG 1 cut(s) 369
NdeII GATC 3 cut(s) 93, 193, 463
NlaIII CATG 4 cut(s) 77, 345, 368, 373
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 427
NsiI ATGCAT 1 cut(s) 109
NspI RCATGY 2 cut(s) 77, 368
PaeI GCATGC 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 377
PfeI GAWTC 2 cut(s) 147, 265
PleI GAGTC 2 cut(s) 344, 369
PpsI GAGTC 2 cut(s) 344, 369
Psp1406I AACGTT 1 cut(s) 321
Psp6I CCWGG 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 447
PspGI CCWGG 1 cut(s) 271
PspN4I GGNNCC 1 cut(s) 195
PstI CTGCAG 1 cut(s) 169
PstNI CAGNNNCTG 2 cut(s) 431, 447
PsuI RGATCY 1 cut(s) 193
RsaI GTAC 1 cut(s) 346
RsaNI GTAC 1 cut(s) 345
SaqAI TTAA 2 cut(s) 129, 318
Sau3AI GATC 3 cut(s) 93, 193, 463
SchI GAGTC 2 cut(s) 344, 369
ScrFI CCNGG 1 cut(s) 273
SduI GDGCHC 1 cut(s) 189
SetI ASST 7 cut(s) 28, 129, 252, 324, 334, 362, 449
SfaNI GCATC 5 cut(s) 94, 116, 267, 394, 424
SfcI CTRYAG 1 cut(s) 165
Sfr274I CTCGAG 1 cut(s) 377
SlaI CTCGAG 1 cut(s) 377
SmlI CTYRAG 2 cut(s) 128, 377
SmoI CTYRAG 2 cut(s) 128, 377
SphI GCATGC 1 cut(s) 368
Sse9I AATT 2 cut(s) 47, 67
SspMI CTAG 3 cut(s) 227, 333, 475
StyD4I CCNGG 1 cut(s) 271
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 217
TaiI ACGT 2 cut(s) 252, 324
TaqI TCGA 4 cut(s) 92, 141, 303, 378
TasI AATT 2 cut(s) 47, 67
TfiI GAWTC 2 cut(s) 147, 265
Tru1I TTAA 2 cut(s) 129, 318
Tru9I TTAA 2 cut(s) 129, 318
TscAI CASTG 3 cut(s) 222, 420, 432
TseFI GTSAC 1 cut(s) 427
Tsp45I GTSAC 1 cut(s) 427
TspDTI ATGAA 2 cut(s) 330, 399
TspGWI ACGGA 1 cut(s) 206
TspRI CASTG 3 cut(s) 222, 420, 432
Vha464I CTTAAG 1 cut(s) 128
XagI CCTNNNNNAGG 1 cut(s) 230
XceI RCATGY 2 cut(s) 77, 368
XhoI CTCGAG 1 cut(s) 377
XspI CTAG 3 cut(s) 227, 333, 475
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.