MD04G1170700.v1.1

Transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0001433
Physical Location & Seq
Forward (+)
26163035 .. 26164620
1586 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1170700.v1.1.491

Sequence Viewer

Length: 459 bp
ATGAGTTCCAAGGAACGAAAGAAAGCAGCTCTGTATGAGAAATTGTTGCAACTTCGTGCTGCCACTAATTCCAGTGCTCTGAACAAAACTTCAATTATCGTTGATGCATCCAAGTATATCGAGGAGTTGAAGCGCAAAGTGGACGCAATGAGACAAGGCGTTGGAACTTCCCAGAATTCAATTGCCCAAAATCCATTACCCGCGGTTACAGTTGAAACCCTAGAAAAGGGTTTCCAAATTCATGTGTTGTCGGAAAAGAATTGCCCGGGTTTGCTCGTCTCCATTCTCGAAGCCTTTGAGGAGCTCGGCCTCGATGTGCTGGATGCTAGGGTTTCTTGTTCAGACACTTTCCAACTAGAAGCTGTTGGAGAAGCGGCGGATAGCATAGATGCTCAGGCGGTGAAAGAAGCAGTCTTGCAAGCTATTCAAACTTGGAGCACAAGCAGTGAGCAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

153

Amino Acids

16.48

Weight (kDa)

4.89

Isoelectric Point (pI)

38.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 69 - 143 2.4e-24 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 203
AciI CCGC 5 cut(s) 201, 203, 374, 377, 398
AcsI RAATTY 2 cut(s) 175, 237
AfiI CCNNNNNNNGG 1 cut(s) 226
AgsI TTSAA 5 cut(s) 93, 130, 180, 215, 428
AluBI AGCT 4 cut(s) 29, 304, 362, 422
AluI AGCT 4 cut(s) 29, 304, 362, 422
Alw21I GWGCWC 3 cut(s) 79, 306, 440
Alw26I GTCTC 2 cut(s) 145, 283
Ama87I CYCGRG 1 cut(s) 265
AoxI GGCC 1 cut(s) 307
ApeKI GCWGC 2 cut(s) 26, 59
ApoI RAATTY 2 cut(s) 175, 237
AspLEI GCGC 1 cut(s) 135
AsuC2I CCSGG 2 cut(s) 266, 267
AsuHPI GGTGA 1 cut(s) 412
AvaI CYCGRG 1 cut(s) 265
BanII GRGCYC 1 cut(s) 306
Bbv12I GWGCWC 3 cut(s) 79, 306, 440
BbvI GCAGC 2 cut(s) 38, 46
BcnI CCSGG 2 cut(s) 266, 267
BcoDI GTCTC 2 cut(s) 145, 283
BfaI CTAG 3 cut(s) 221, 327, 356
BisI GCNGC 3 cut(s) 27, 60, 375
BlsI GCNGC 3 cut(s) 28, 61, 376
Bme1390I CCNGG 2 cut(s) 266, 267
BmeT110I CYCGRG 1 cut(s) 265
BmrFI CCNGG 2 cut(s) 266, 267
BmsI GCATC 4 cut(s) 94, 116, 313, 379
Bpu10I CCTNAGC 1 cut(s) 393
BpuMI CCSGG 2 cut(s) 266, 267
BsaJI CCNNGG 3 cut(s) 9, 201, 265
Bsc4I CCNNNNNNNGG 1 cut(s) 226
Bse1I ACTGG 1 cut(s) 72
Bse3DI GCAATG 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 9, 201, 265
BseGI GGATG 2 cut(s) 107, 328
BseLI CCNNNNNNNGG 1 cut(s) 226
BseMI GCAATG 1 cut(s) 153
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 1 cut(s) 72
BseRI GAGGAG 2 cut(s) 137, 314
BseXI GCAGC 2 cut(s) 38, 46
Bsh1236I CGCG 1 cut(s) 203
BshFI GGCC 1 cut(s) 309
BsiHKAI GWGCWC 3 cut(s) 79, 306, 440
BsiHKCI CYCGRG 1 cut(s) 265
BsiSI CCGG 1 cut(s) 266
BslI CCNNNNNNNGG 1 cut(s) 226
BsmAI GTCTC 2 cut(s) 145, 283
BsmBI CGTCTC 1 cut(s) 283
BsnI GGCC 1 cut(s) 309
BsoBI CYCGRG 1 cut(s) 265
Bsp1286I GDGCHC 3 cut(s) 79, 306, 440
BspACI CCGC 5 cut(s) 201, 203, 374, 377, 398
BspANI GGCC 1 cut(s) 309
BspCNI CTCAG 1 cut(s) 406
BspFNI CGCG 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 153
BsrI ACTGG 1 cut(s) 72
BssECI CCNNGG 3 cut(s) 9, 201, 265
BssT1I CCWWGG 1 cut(s) 9
Bst4CI ACNGT 1 cut(s) 211
BstC8I GCNNGC 1 cut(s) 420
BstDEI CTNAG 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 201
BstENI CCTNNNNNAGG 1 cut(s) 224
BstF5I GGATG 2 cut(s) 107, 328
BstFNI CGCG 1 cut(s) 203
BstHHI GCGC 1 cut(s) 135
BstMAI GTCTC 2 cut(s) 145, 283
BstSCI CCNGG 2 cut(s) 264, 265
BstUI CGCG 1 cut(s) 203
BstV1I GCAGC 2 cut(s) 38, 46
BsuRI GGCC 1 cut(s) 309
BtgI CCRYGG 1 cut(s) 201
BtsCI GGATG 2 cut(s) 107, 328
BtsI GCAGTG 1 cut(s) 451
BtsIMutI CAGTG 2 cut(s) 79, 451
Cac8I GCNNGC 1 cut(s) 420
CfoI GCGC 1 cut(s) 135
Cfr42I CCGCGG 1 cut(s) 204
Cfr9I CCCGGG 1 cut(s) 265
CseI GACGC 1 cut(s) 152
CviAII CATG 1 cut(s) 242
CviJI RGCY 6 cut(s) 29, 293, 304, 309, 362, 422
CviKI_1 RGCY 6 cut(s) 29, 293, 304, 309, 362, 422
DdeI CTNAG 1 cut(s) 393
EciI GGCGGA 1 cut(s) 392
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 1 cut(s) 9
Eco24I GRGCYC 1 cut(s) 306
Eco53kI GAGCTC 1 cut(s) 304
Eco88I CYCGRG 1 cut(s) 265
EcoICRI GAGCTC 1 cut(s) 304
EcoNI CCTNNNNNAGG 1 cut(s) 224
EcoRI GAATTC 1 cut(s) 175
EcoT14I CCWWGG 1 cut(s) 9
EcoT22I ATGCAT 1 cut(s) 109
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 1 cut(s) 9
Esp3I CGTCTC 1 cut(s) 283
FaeI CATG 1 cut(s) 245
FaiI YATR 4 cut(s) 36, 117, 243, 386
FatI CATG 1 cut(s) 241
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 3 cut(s) 27, 60, 375
FokI GGATG 2 cut(s) 94, 335
FriOI GRGCYC 1 cut(s) 306
Fsp4HI GCNGC 3 cut(s) 27, 60, 375
FspBI CTAG 3 cut(s) 221, 327, 356
GlaI GCGC 1 cut(s) 134
GluI GCNGC 3 cut(s) 27, 60, 375
HaeIII GGCC 1 cut(s) 309
HapII CCGG 1 cut(s) 266
HgaI GACGC 1 cut(s) 152
HhaI GCGC 1 cut(s) 135
Hin1II CATG 1 cut(s) 245
Hin6I GCGC 1 cut(s) 133
HinP1I GCGC 1 cut(s) 133
HpaII CCGG 1 cut(s) 266
HphI GGTGA 1 cut(s) 412
Hpy166II GTNNAC 1 cut(s) 142
Hpy188I TCNGA 3 cut(s) 81, 253, 343
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 142
HpyCH4III ACNGT 1 cut(s) 211
HpyCH4V TGCA 3 cut(s) 49, 107, 418
HpyF3I CTNAG 1 cut(s) 393
Hsp92II CATG 1 cut(s) 245
HspAI GCGC 1 cut(s) 133
KspI CCGCGG 1 cut(s) 204
LmnI GCTCC 2 cut(s) 301, 435
LpnPI CCDG 5 cut(s) 85, 185, 279, 305, 380
Lsp1109I GCAGC 2 cut(s) 38, 46
LweI GCATC 4 cut(s) 94, 116, 313, 379
MaeI CTAG 3 cut(s) 221, 327, 356
MaeIII GTNAC 1 cut(s) 205
MfeI CAATTG 1 cut(s) 180
MhlI GDGCHC 3 cut(s) 79, 306, 440
MluCI AATT 7 cut(s) 41, 67, 93, 175, 180, 237, 259
MmeI TCCRAC 4 cut(s) 142, 231, 346, 376
MnlI CCTC 3 cut(s) 115, 292, 320
Mph1103I ATGCAT 1 cut(s) 109
MseI TTAA 1 cut(s) 457
MspA1I CMGCKG 1 cut(s) 203
MspI CCGG 1 cut(s) 266
MspR9I CCNGG 2 cut(s) 266, 267
MunI CAATTG 1 cut(s) 180
MvnI CGCG 1 cut(s) 203
NciI CCSGG 2 cut(s) 266, 267
NlaIII CATG 1 cut(s) 245
NmeAIII GCCGAG 1 cut(s) 285
NsiI ATGCAT 1 cut(s) 109
PkrI GCNGC 3 cut(s) 28, 61, 376
Psp124BI GAGCTC 1 cut(s) 306
SacI GAGCTC 1 cut(s) 306
SacII CCGCGG 1 cut(s) 204
SaqAI TTAA 1 cut(s) 457
SatI GCNGC 3 cut(s) 27, 60, 375
ScrFI CCNGG 2 cut(s) 266, 267
SduI GDGCHC 3 cut(s) 79, 306, 440
SetI ASST 4 cut(s) 31, 306, 364, 424
SfaNI GCATC 4 cut(s) 94, 116, 313, 379
Sfr303I CCGCGG 1 cut(s) 204
SgrBI CCGCGG 1 cut(s) 204
SmaI CCCGGG 1 cut(s) 267
Sse9I AATT 7 cut(s) 41, 67, 93, 175, 180, 237, 259
SsiI CCGC 5 cut(s) 201, 203, 374, 377, 398
SspMI CTAG 3 cut(s) 221, 327, 356
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 2 cut(s) 264, 265
StyI CCWWGG 1 cut(s) 9
TaaI ACNGT 1 cut(s) 211
TaqI TCGA 3 cut(s) 120, 288, 312
TasI AATT 7 cut(s) 41, 67, 93, 175, 180, 237, 259
TauI GCSGC 1 cut(s) 377
Tru1I TTAA 1 cut(s) 457
Tru9I TTAA 1 cut(s) 457
TscAI CASTG 2 cut(s) 79, 451
TseI GCWGC 2 cut(s) 26, 59
TspDTI ATGAA 1 cut(s) 230
TspMI CCCGGG 1 cut(s) 265
TspRI CASTG 2 cut(s) 79, 451
XagI CCTNNNNNAGG 1 cut(s) 224
XapI RAATTY 2 cut(s) 175, 237
XmaI CCCGGG 1 cut(s) 265
XspI CTAG 3 cut(s) 221, 327, 356
Zsp2I ATGCAT 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.