pycom07g20770

transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0001433
Physical Location & Seq
Forward (+)
22749344 .. 22751077
1734 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g20770.1

Sequence Viewer

Length: 483 bp
ATGGTTTCTAGGGAGCACAAGAGGGCAGCACTCTATGAAAAGCTGCAACTTCTTCGTTCTATTACTAACTCTCATGCTCTAAACAAAACCTCCATCATAGTAGATGCCTCCAAGTACATTGAAGAGCTAAAACAAAAGGTGGAAAGACTGAATCAAGATATTGTAAATGCACAAACTACTTCATCATCAAGTGACCAAACTTCGTTGCCTGTGCAGGTTACAGTGGAAACCCTAGAAAAAGGATTTCTGATAAATGCGTTTTCTGAGAAGAGTTGCCCAGGTTTGCTTGTGTCCGTTCTGGAAGCTTTCGAAGACTTGGGTCTCAATGTGCTTGAAGCTAGGGTTTCCTGTGCAGACAGTTTCAGGTTACAGGCAGTTGGAGGAGAAAATGAAGAGGAAGGTGAAAGCATTGACGCTCATGCAGTAAAGCAAGCAGTGGCAGTGGCTATAAAAAACTGGAGTGAAAATACCGAAAATGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

17.6

Weight (kDa)

4.89

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
bHLH-TF_ACT-like_plant PF22754 72 - 151 4.3e-24 Plant bHLH transcription factor, ACT-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 205
AfaI GTAC 1 cut(s) 116
AgsI TTSAA 2 cut(s) 122, 335
AjnI CCWGG 1 cut(s) 277
AluBI AGCT 4 cut(s) 43, 127, 305, 338
AluI AGCT 4 cut(s) 43, 127, 305, 338
Alw21I GWGCWC 1 cut(s) 18
Alw26I GTCTC 1 cut(s) 326
ApeKI GCWGC 2 cut(s) 26, 43
AsuHPI GGTGA 1 cut(s) 413
AsuII TTCGAA 1 cut(s) 309
BbsI GAAGAC 1 cut(s) 318
Bbv12I GWGCWC 1 cut(s) 18
BbvI GCAGC 2 cut(s) 30, 38
BccI CCATC 1 cut(s) 101
BcgI CGANNNNNNTGC 2 cut(s) 35, 69
BciT130I CCWGG 1 cut(s) 279
BcoDI GTCTC 1 cut(s) 326
BfaI CTAG 3 cut(s) 9, 233, 339
BfuAI ACCTGC 1 cut(s) 205
BisI GCNGC 2 cut(s) 27, 44
BlsI GCNGC 2 cut(s) 28, 45
Bme1390I CCNGG 1 cut(s) 279
BmrFI CCNGG 1 cut(s) 279
BmsI GCATC 1 cut(s) 94
BoxI GACNNNNGTC 1 cut(s) 318
BpiI GAAGAC 1 cut(s) 318
BpmI CTGGAG 1 cut(s) 478
Bpu14I TTCGAA 1 cut(s) 309
BsaI GGTCTC 1 cut(s) 326
BsaJI CCNNGG 1 cut(s) 277
BsaXI ACNNNNNCTCC 2 cut(s) 74, 104
Bse1I ACTGG 1 cut(s) 461
BseBI CCWGG 1 cut(s) 279
BseDI CCNNGG 1 cut(s) 277
BseMII CTCAG 1 cut(s) 255
BseNI ACTGG 1 cut(s) 461
BseRI GAGGAG 1 cut(s) 396
BseXI GCAGC 2 cut(s) 30, 38
BsgI GTGCAG 2 cut(s) 233, 372
BsiHKAI GWGCWC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 326
Bso31I GGTCTC 1 cut(s) 326
Bsp119I TTCGAA 1 cut(s) 309
Bsp1286I GDGCHC 1 cut(s) 18
BspCNI CTCAG 1 cut(s) 256
BspMI ACCTGC 1 cut(s) 205
BspQI GCTCTTC 1 cut(s) 117
BspT104I TTCGAA 1 cut(s) 309
BspTNI GGTCTC 1 cut(s) 326
BsrI ACTGG 1 cut(s) 461
BssECI CCNNGG 1 cut(s) 277
Bst2UI CCWGG 1 cut(s) 279
Bst4CI ACNGT 2 cut(s) 223, 359
Bst6I CTCTTC 3 cut(s) 117, 263, 387
BstBI TTCGAA 1 cut(s) 309
BstC8I GCNNGC 1 cut(s) 432
BstDEI CTNAG 1 cut(s) 264
BstMAI GTCTC 1 cut(s) 326
BstNI CCWGG 1 cut(s) 279
BstPAI GACNNNNGTC 1 cut(s) 318
BstSCI CCNGG 1 cut(s) 277
BstV1I GCAGC 2 cut(s) 30, 38
BstV2I GAAGAC 1 cut(s) 318
BtsI GCAGTG 2 cut(s) 441, 447
BtsIMutI CAGTG 3 cut(s) 228, 441, 447
BveI ACCTGC 1 cut(s) 205
Cac8I GCNNGC 1 cut(s) 432
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 115
CviAII CATG 2 cut(s) 74, 419
CviJI RGCY 5 cut(s) 43, 127, 305, 338, 446
CviKI_1 RGCY 5 cut(s) 43, 127, 305, 338, 446
CviQI GTAC 1 cut(s) 115
DdeI CTNAG 1 cut(s) 264
Eam1104I CTCTTC 3 cut(s) 117, 263, 387
EarI CTCTTC 3 cut(s) 117, 263, 387
Eco31I GGTCTC 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 277
FaeI CATG 2 cut(s) 77, 422
FaiI YATR 5 cut(s) 36, 75, 98, 420, 449
FatI CATG 2 cut(s) 73, 418
Fnu4HI GCNGC 2 cut(s) 27, 44
Fsp4HI GCNGC 2 cut(s) 27, 44
FspBI CTAG 3 cut(s) 9, 233, 339
GluI GCNGC 2 cut(s) 27, 44
GsuI CTGGAG 1 cut(s) 478
HgaI GACGC 1 cut(s) 422
Hin1II CATG 2 cut(s) 77, 422
HindIII AAGCTT 1 cut(s) 303
HinfI GANTC 1 cut(s) 151
HphI GGTGA 1 cut(s) 413
Hpy188I TCNGA 2 cut(s) 249, 265
Hpy188III TCNNGA 2 cut(s) 155, 299
HpyAV CCTTC 1 cut(s) 392
HpyCH4III ACNGT 2 cut(s) 223, 359
HpyCH4V TGCA 5 cut(s) 46, 170, 214, 353, 422
HpyF3I CTNAG 1 cut(s) 264
Hsp92II CATG 2 cut(s) 77, 422
LguI GCTCTTC 1 cut(s) 117
LmnI GCTCC 1 cut(s) 13
LpnPI CCDG 9 cut(s) 200, 222, 264, 284, 291, 349, 356, 361, 442
Lsp1109I GCAGC 2 cut(s) 30, 38
LweI GCATC 1 cut(s) 94
MaeI CTAG 3 cut(s) 9, 233, 339
MaeIII GTNAC 3 cut(s) 191, 217, 366
MboII GAAGA 5 cut(s) 44, 134, 280, 323, 404
MhlI GDGCHC 1 cut(s) 18
MmeI TCCRAC 1 cut(s) 358
MnlI CCTC 5 cut(s) 15, 100, 118, 374, 388
MspR9I CCNGG 1 cut(s) 279
MvaI CCWGG 1 cut(s) 279
NlaIII CATG 2 cut(s) 77, 422
NmuCI GTSAC 1 cut(s) 191
NspV TTCGAA 1 cut(s) 309
PciSI GCTCTTC 1 cut(s) 117
PfeI GAWTC 1 cut(s) 151
PkrI GCNGC 2 cut(s) 28, 45
PshAI GACNNNNGTC 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 277
PspGI CCWGG 1 cut(s) 277
RsaI GTAC 1 cut(s) 116
RsaNI GTAC 1 cut(s) 115
SapI GCTCTTC 1 cut(s) 117
SatI GCNGC 2 cut(s) 27, 44
ScrFI CCNGG 1 cut(s) 279
SduI GDGCHC 1 cut(s) 18
SfaNI GCATC 1 cut(s) 94
SfuI TTCGAA 1 cut(s) 309
SspMI CTAG 3 cut(s) 9, 233, 339
StyD4I CCNGG 1 cut(s) 277
TaaI ACNGT 2 cut(s) 223, 359
TaqI TCGA 1 cut(s) 309
TatI WGTACW 1 cut(s) 114
TfiI GAWTC 1 cut(s) 151
TscAI CASTG 3 cut(s) 228, 441, 447
TseFI GTSAC 1 cut(s) 191
TseI GCWGC 2 cut(s) 26, 43
Tsp45I GTSAC 1 cut(s) 191
TspDTI ATGAA 3 cut(s) 51, 171, 405
TspGWI ACGGA 1 cut(s) 283
TspRI CASTG 3 cut(s) 228, 441, 447
XspI CTAG 3 cut(s) 9, 233, 339
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.